Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,503,262 G→T 26.4% L20L (CTC→CTA)  yncJ ← uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,503,2620GT26.4% 676.6 / 187.1 309L20L (CTC→CTA) yncJuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (95/131);  new base T (35/47);  total (130/179)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 310 total aligned reads displayed.

TTGCGCGATTTTTGCCAGTAAAAATGCTCGCGCAAACCTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTATTATTCAGTTTTGCAAATTAGCGCAAAGAA  >  CP009273/1503113‑1503407
                                                                                                                                                     |                                                                                                                                                 
   cgcgATTTTTGCCAGTAAAAATGCTCGCGCAAACCTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGt                                                                                                                                                >  2:2869479/1‑150 (MQ=255)
     cgATTTTTGCCAGTAAAAATGCTCGCGCAAACCTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTg                                                                                                                                              >  2:3984721/1‑150 (MQ=255)
     cgATTTTTGCCAGTAAAAATGCTCGCGCAAACCTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTg                                                                                                                                              <  1:339327/150‑1 (MQ=255)
          tttGCCAGTAAAAATGCTCGCGCAAACCTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTg                                                                                                                                         >  1:882726/1‑150 (MQ=255)
          tttGCCAGTAAAAATGCTCGCGCAAACCTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTg                                                                                                                                         <  2:1535375/150‑1 (MQ=255)
             gCCAGTAAAAATGCTCGCGCAAACCTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGaaa                                                                                                                                      <  2:4262095/150‑1 (MQ=255)
               cAGTAAAAATGCTCGCGCAAACCTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAAc                                                                                                                                    <  1:4679757/150‑1 (MQ=255)
                    aaaaTGCTCGCGCAAACCTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGc                                                                                                                               >  1:2887188/1‑150 (MQ=255)
                    aaaaTGCTCGCGCAAACCTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGc                                                                                                                               >  1:2176365/1‑150 (MQ=255)
                     aaaTGCTCGCGCAAACCTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGca                                                                                                                              >  1:1215822/1‑150 (MQ=255)
                       aTGCTCGCGCAAACCTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGcaca                                                                                                                            <  1:3794451/150‑1 (MQ=255)
                            cgcgCAAACCTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTta                                                                                                                       <  1:643064/150‑1 (MQ=255)
                               gcAAACCTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTtaata                                                                                                                    <  1:2728425/150‑1 (MQ=255)
                               gcAAACCTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAATCAGAGCACACGTtaaga                                                                                                                    >  2:2795081/1‑148 (MQ=255)
                                cAAACCTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTtaataa                                                                                                                   <  1:3220557/150‑1 (MQ=255)
                                 aaaCCTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAg                                                                                                                  <  2:1638725/150‑1 (MQ=255)
                                 aaaCCTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAg                                                                                                                  >  2:2370174/1‑150 (MQ=255)
                                   aCCTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGAc                                                                                                                <  2:3086687/150‑1 (MQ=255)
                                     cTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTGACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACaa                                                                                                              >  2:5113692/1‑150 (MQ=255)
                                     cTTCCGCCGACTCTTCCGCCACAGCACGCAAGTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACaa                                                                                                              <  2:3028114/150‑1 (MQ=255)
                                       tCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAAcc                                                                                                            <  1:4318597/150‑1 (MQ=255)
                                          gccgACTCTTCCGCCACAGCACGCAATTCATCGATGTCCGCTTCATGACGCATCTGATGATCCACATTCTTTACCCACACAAAATCATGACCTTAGAGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGAt                                                                                                         >  1:3959403/1‑150 (MQ=255)
                                            cgACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATaa                                                                                                       <  2:3160108/150‑1 (MQ=255)
                                            cgACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATaa                                                                                                       >  2:5107327/1‑150 (MQ=255)
                                              aCTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACg                                                                                                     <  1:1365680/150‑1 (MQ=255)
                                                 ctTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCt                                                                                                  <  1:1840513/150‑1 (MQ=255)
                                                 ctTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCt                                                                                                  >  2:2475355/1‑150 (MQ=255)
                                                 caaacGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGACCTGCCATGAGTAGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGcca                                                                                                  >  2:3577424/5‑149 (MQ=255)
                                                  ttCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACCCAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCtt                                                                                                 <  2:2051876/150‑1 (MQ=255)
                                                  ttCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCtt                                                                                                 >  2:1464483/1‑150 (MQ=255)
                                                  ttCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCtt                                                                                                 <  1:2051217/150‑1 (MQ=255)
                                                   tCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTc                                                                                                <  2:3427141/150‑1 (MQ=255)
                                                   tCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTc                                                                                                <  2:2709438/150‑1 (MQ=255)
                                                     cGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGt                                                                                              <  2:262979/150‑1 (MQ=255)
                                                      gCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTa                                                                                             >  2:4816032/1‑150 (MQ=255)
                                                      gCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTa                                                                                             <  2:4427102/150‑1 (MQ=255)
                                                        cacaGCACGCACTTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTaaa                                                                                           <  1:4676467/150‑1 (MQ=255)
                                                         acaGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAAc                                                                                          <  2:5027326/150‑1 (MQ=255)
                                                         acaGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAAc                                                                                          <  1:3098248/150‑1 (MQ=255)
                                                             cACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATcc                                                                                      >  2:4043417/1‑150 (MQ=255)
                                                              aCGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCt                                                                                     <  1:265997/150‑1 (MQ=255)
                                                               cGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTg                                                                                    >  2:4622503/1‑150 (MQ=255)
                                                                gCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGc                                                                                   <  2:48909/150‑1 (MQ=255)
                                                                 cAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGcc                                                                                  >  1:4866865/1‑150 (MQ=255)
                                                                     tCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCt                                                                              <  2:2570558/150‑1 (MQ=255)
                                                                       aTCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTGACCCACACAACTTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCttt                                                                            <  2:2758187/150‑1 (MQ=255)
                                                                         cGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCttttt                                                                          >  1:4771102/1‑150 (MQ=255)
                                                                          gCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTc                                                                         <  1:5049046/150‑1 (MQ=255)
                                                                            tGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCtt                                                                       <  1:5156540/150‑1 (MQ=255)
                                                                               ccGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCTAACGTTAATAAGACAACAGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTAcc                                                                    <  1:4453089/150‑1 (MQ=255)
                                                                                cGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCt                                                                   <  2:529932/150‑1 (MQ=255)
                                                                                cGCTTCATGACGCAGCTGATGATCCACATTCTCTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCt                                                                   <  2:2302299/150‑1 (MQ=255)
                                                                                 gCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCtt                                                                  <  2:1818981/150‑1 (MQ=255)
                                                                                  cTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCttt                                                                 >  1:3642032/1‑150 (MQ=255)
                                                                                   ttCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCtttt                                                                <  1:2872508/150‑1 (MQ=255)
                                                                                     cATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGc                                                              >  2:755474/1‑150 (MQ=255)
                                                                                     cATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATCAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGc                                                              <  1:4221997/150‑1 (MQ=255)
                                                                                      aTGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGcc                                                             <  2:2646077/150‑1 (MQ=255)
                                                                                         aCGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCt                                                          <  1:1594769/150‑1 (MQ=255)
                                                                                         aCGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCt                                                          <  2:1215822/150‑1 (MQ=255)
                                                                                          cGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTa                                                         >  2:4398696/1‑150 (MQ=255)
                                                                                             aGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTatga                                                      <  1:4951204/150‑1 (MQ=255)
                                                                                             aGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTatga                                                      >  1:1597478/1‑150 (MQ=255)
                                                                                              gCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTatgat                                                     <  1:775001/150‑1 (MQ=255)
                                                                                              gCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTatgat                                                     >  2:1018955/1‑150 (MQ=255)
                                                                                                tgatgaTCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACC‑TTTTCTTACCTTTTGCCGCTATGATGcc                                                  <  1:1000736/150‑1 (MQ=255)
                                                                                                 gatgatCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGcc                                                  <  1:1455196/150‑1 (MQ=255)
                                                                                                  atgatCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCg                                                 >  1:3873691/1‑150 (MQ=255)
                                                                                                  atgatCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCg                                                 >  2:1116133/1‑150 (MQ=255)
                                                                                                     atCCACATTCTTTACCCAGACTAATTCATTTCCTTTGTGCCCTGCCTTGAGTTGTGCTGAAAACTGAGCAGACGTTTATAAGACTTCCGTTTACGCCTTCGTAAACATCCTGCCTCATTTTTCTTTCCTTGTGCCGCTATGATGCCGATc                                              <  2:327593/150‑1 (MQ=255)
                                                                                                     atCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATc                                              <  1:1709962/150‑1 (MQ=255)
                                                                                                     atCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATc                                              <  2:5272036/150‑1 (MQ=255)
                                                                                                       ccACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGt                                            >  1:5222858/1‑150 (MQ=255)
                                                                                                       ccACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGt                                            >  1:3268510/1‑150 (MQ=255)
                                                                                                       ccACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGACGCTATGATGCCGAGCGt                                            >  1:2773144/1‑150 (MQ=255)
                                                                                                        cacaTTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGtt                                           <  1:2546910/150‑1 (MQ=255)
                                                                                                          caTTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTc                                         <  1:1776854/150‑1 (MQ=255)
                                                                                                           aTTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCt                                        <  2:694515/150‑1 (MQ=255)
                                                                                                           aTTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCt                                        <  1:1561273/150‑1 (MQ=255)
                                                                                                           aTTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCt                                        <  2:4769003/150‑1 (MQ=255)
                                                                                                             tCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTg                                      <  1:3577340/150‑1 (MQ=255)
                                                                                                               tttACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCAGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAg                                    <  1:5318648/150‑1 (MQ=255)
                                                                                                                    ccACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGttat                               <  2:4183218/150‑1 (MQ=255)
                                                                                                                      acacaAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGttatta                             <  2:4907444/150‑1 (MQ=255)
                                                                                                                         caAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCAATTTCTTACCATTTGCCGCTATGATGCCGATCGTTTCTTGAGGTAATTATTc                          >  2:2397746/1‑150 (MQ=255)
                                                                                                                          aaaTTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTATTATTCa                         <  1:2033402/150‑1 (MQ=255)
                                                                                                                          aaaTTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTATTATTCa                         >  1:2245079/1‑150 (MQ=255)
                                                                                                                           aaTTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTATTATTCAg                        >  1:709010/1‑150 (MQ=255)
                                                                                                                            aTTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGATGTTATTATTCAGt                       >  1:1922718/1‑150 (MQ=255)
                                                                                                                            aTTCATGTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATAGTTTCTTGAGGGTATTATTCAGt                       >  2:877308/1‑150 (MQ=255)
                                                                                                                              tCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTATTATTCAGttt                     >  1:3721442/1‑150 (MQ=255)
                                                                                                                               cATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTATTATTCAGtttt                    <  2:378873/150‑1 (MQ=255)
                                                                                                                               cATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTATTATTCAGtttt                    <  1:2948271/150‑1 (MQ=255)
                                                                                                                                  gTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTCTTATTCAGTTTTGCa                 >  2:1729829/1‑150 (MQ=255)
                                                                                                                                  gTCCTTTGTGCCCTGCCATTAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTATTATTCAGTTTTGCa                 <  1:2371053/150‑1 (MQ=255)
                                                                                                                                       ttGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAAGAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTATTATTCAGTTTTGCAAATTa            <  2:2682999/150‑1 (MQ=255)
                                                                                                                                        tgtgCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTATTATTCAGTTTTGCAAATTAg           <  2:3301009/150‑1 (MQ=255)
                                                                                                                                           gCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTATTATTCAGTTTTGCAAATTAgcgc        <  2:3905296/150‑1 (MQ=255)
                                                                                                                                               tGCCATGAGTTGTCCTGATAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTATTATTCAGTTTTGCAAATTAGCGCAAAg    <  1:35609/150‑1 (MQ=255)
                                                                                                                                                 ccATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTATTATTCAGTTTTGCAAATTAGCGCAAAGaa  <  1:4268292/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TTGCGCGATTTTTGCCAGTAAAAATGCTCGCGCAAACCTTCCGCCGACTCTTCCGCCACAGCACGCAATTCATCGCTGTCCGCTTCATGACGCAGCTGATGATCCACATTCTTTACCCACACAAATTCATGTCCTTTGTGCCCTGCCATGAGTTGTCCTGAAAACAGAGCACACGTTAATAAGACAACCGATAACGCCTTCGTAAACATCCTGCCACCTTTTTCTTACCTTTTGCCGCTATGATGCCGATCGTTTCTTGAGGTTATTATTCAGTTTTGCAAATTAGCGCAAAGAA  >  CP009273/1503113‑1503407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑