BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,512,106	G→T	52.5%	E68* (GAA→TAA)	ydcY →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,512,106	0	G	T	52.5%	66.1 / inf	291	E68* (GAA→TAA)	ydcY	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (76/62); new base T (68/84); total (145/146)
Fisher's exact test for biased strand distribution p-value = 9.96e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01

Only 100 of 294 total aligned reads displayed.

GTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGATATCCAGCATCAG  >  CP009273/1511959‑1512255

 tCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAAc                                                                                                                                                    >  1:3870556/1‑150 (MQ=255)

  cATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACa                                                                                                                                                   >  2:1390914/1‑150 (MQ=255)

   aTTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAg                                                                                                                                                  <  1:4576506/150‑1 (MQ=255)

       cccATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTaa                                                                                                                                              >  1:4810424/1‑150 (MQ=255)

        ccATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAAc                                                                                                                                             <  1:4180151/150‑1 (MQ=255)

           tatGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCaa                                                                                                                                          >  2:3573777/1‑150 (MQ=255)

            atGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCaaa                                                                                                                                         >  1:2638382/1‑150 (MQ=255)

             tGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAg                                                                                                                                        <  1:4692282/150‑1 (MQ=255)

               aaCGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAggc                                                                                                                                      >  1:4429434/1‑150 (MQ=255)

                aCGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAggcg                                                                                                                                     <  2:3366942/150‑1 (MQ=255)

                    attattAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACATCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAAACAAAGGCGGCAc                                                                                                                                 <  2:4085070/150‑1 (MQ=255)

                    attattAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCAc                                                                                                                                 >  2:3241051/1‑150 (MQ=255)

                     ttattaATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCAcc                                                                                                                                >  1:92765/1‑150 (MQ=255)

                       attaATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCAt                                                                                                                              <  1:4227390/150‑1 (MQ=255)

                       attaATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCAt                                                                                                                              >  1:4014634/1‑150 (MQ=255)

                          aagcGCCCTGCGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCct                                                                                                                           <  1:2252218/147‑1 (MQ=255)

                          aaTCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAACGGCGGCACCATCct                                                                                                                           <  1:742553/150‑1 (MQ=255)

                           aTCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCctc                                                                                                                          >  2:1267431/1‑150 (MQ=255)

                           aTCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCctc                                                                                                                          >  1:2585276/1‑150 (MQ=255)

                            tCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCctct                                                                                                                         <  2:2413512/150‑1 (MQ=255)

                             cGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTg                                                                                                                        >  2:2489523/1‑150 (MQ=255)

                               cccTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGAt                                                                                                                      >  2:1432177/1‑150 (MQ=255)

                                ccTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGAtt                                                                                                                     >  2:3712416/1‑150 (MQ=255)

                                   gAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAATATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTaaa                                                                                                                  >  2:1291721/1‑150 (MQ=255)

                                    aGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTaaaa                                                                                                                 <  2:2732440/150‑1 (MQ=255)

                                     gCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAAt                                                                                                                <  2:943351/150‑1 (MQ=255)

                                       gATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGa                                                                                                              >  2:414468/1‑150 (MQ=255)

                                       gATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGa                                                                                                              >  2:640823/1‑150 (MQ=255)

                                            cgcgGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTc                                                                                                         >  2:382561/1‑150 (MQ=255)

                                             gcgGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCt                                                                                                        <  2:4266730/150‑1 (MQ=255)

                                              cgGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTg                                                                                                       >  1:585548/1‑150 (MQ=255)

                                              cgGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTg                                                                                                       <  1:2868786/150‑1 (MQ=255)

                                               ggAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTgg                                                                                                      <  1:3352698/150‑1 (MQ=255)

                                                    tAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTacc                                                                                                 <  2:4900376/150‑1 (MQ=255)

                                                        tCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTaccaacc                                                                                             <  2:5163336/150‑1 (MQ=255)

                                                         cGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTaccaacca                                                                                            <  2:1650727/150‑1 (MQ=255)

                                                         cGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTaccaacca                                                                                            <  1:36867/150‑1 (MQ=255)

                                                               gATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGcc                                                                                      >  1:3012846/1‑150 (MQ=255)

                                                                 tCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCaa                                                                                    >  1:4367490/1‑150 (MQ=255)

                                                                   gCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGACGCGCGCCACGTACAGTTCACCAAAGGCGGCACCATCCTCGGATTACAATGAGAGTCTGGCGACCAACCAGGCGCCAAcc                                                                                  >  2:5050611/1‑150 (MQ=255)

                                                                     tACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAATATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACcac                                                                                >  1:2528643/1‑150 (MQ=255)

                                                                      acacCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACcaca                                                                               <  2:1828690/150‑1 (MQ=255)

                                                                       cacCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACaa                                                                              >  1:702318/1‑150 (MQ=255)

                                                                       cacCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACaa                                                                              <  2:4462365/150‑1 (MQ=255)

                                                                       cacCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACaa                                                                              <  1:211254/150‑1 (MQ=255)

                                                                          ccAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAATATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTa                                                                           <  2:5215008/150‑1 (MQ=255)

                                                                          ccAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTa                                                                           <  1:1158979/150‑1 (MQ=255)

                                                                            agcagcAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTaaa                                                                         >  2:902455/1‑150 (MQ=255)

                                                                              cagcaACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAAt                                                                       <  2:4474418/150‑1 (MQ=255)

                                                                               agcaACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAAtt                                                                      <  2:289345/150‑1 (MQ=255)

                                                                                gcaACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTg                                                                     >  1:2786096/1‑150 (MQ=255)

                                                                                  aaCGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGTGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTgcg                                                                   >  1:4130122/1‑150 (MQ=255)

                                                                                     gTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCca                                                                >  1:1106723/1‑150 (MQ=255)

                                                                                         gcgcACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACaaa                                                            <  2:1601523/150‑1 (MQ=255)

                                                                                          cgcACAGCGATCGCCCATCACGGTCGCAGGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAAt                                                           >  2:1236736/1‑150 (MQ=255)

                                                                                          cgcACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAAt                                                           <  1:3070117/150‑1 (MQ=255)

                                                                                              caGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTggg                                                       >  1:3948189/1‑150 (MQ=255)

                                                                                              caGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTggg                                                       <  1:353277/150‑1 (MQ=255)

                                                                                              caGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTggg                                                       <  2:267402/150‑1 (MQ=255)

                                                                                              caGCGATCGCCAATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTggg                                                       <  1:3141028/150‑1 (MQ=255)

                                                                                                 cGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCg                                                    <  2:3154492/150‑1 (MQ=255)

                                                                                                 cGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCg                                                    <  1:2277963/150‑1 (MQ=255)

                                                                                                    tCGCCCATCACGGTCGCAAGCATAAAGAAGTTATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACATGTAAAATTGCGCCACAAATTGGGCCGaac                                                 <  2:2219533/150‑1 (MQ=255)

                                                                                                    tCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGaac                                                 <  1:3231947/150‑1 (MQ=255)

                                                                                                       cccATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGaacaac                                              <  1:4317508/150‑1 (MQ=255)

                                                                                                        ccATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACg                                             <  2:3662314/150‑1 (MQ=255)

                                                                                                           tCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGccc                                          >  1:1316035/1‑150 (MQ=255)

                                                                                                             aCGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGt                                        >  2:3469106/1‑150 (MQ=255)

                                                                                                              cGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTa                                       >  1:1024720/1‑150 (MQ=255)

                                                                                                                gTCGAAATCATAAATAATATATGGAAGCGCGCCAATAACATTAAACCAACGGCGGCACCATCATCTTATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCAAAATTAAAATGGCGCCACAAATTGGGCCGAACAACGCCCGTAGt                                     <  1:4970707/150‑1 (MQ=255)

                                                                                                                   gCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGt                                  <  1:3844754/150‑1 (MQ=255)

                                                                                                                   gCAAGCATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGt                                  <  1:1489040/150‑1 (MQ=255)

                                                                                                                     aaGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTAc                                <  2:4014618/150‑1 (MQ=255)

                                                                                                                     aaGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCAAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTAc                                <  2:932023/150‑1 (MQ=255)

                                                                                                                       gCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGc                              >  2:4799903/1‑150 (MQ=255)

                                                                                                                       gCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCGGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGc                              >  1:2966647/1‑150 (MQ=255)

                                                                                                                        cATAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAACGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGcc                             <  1:1963032/150‑1 (MQ=255)

                                                                                                                        cATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGcc                             >  1:3476061/1‑150 (MQ=255)

                                                                                                                        cATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGcc                             >  2:1789053/1‑150 (MQ=255)

                                                                                                                        cATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGcc                             <  2:4103332/150‑1 (MQ=255)

                                                                                                                          tAAAGAAGATATGGAAGCGCGCCACTAACCGTTAACCAACGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCAt                           <  1:242985/150‑1 (MQ=255)

                                                                                                                          tAAAGAAGATATGGAAGCGCGCCACTAACAGTTAACCAACGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCAt                           <  1:2928571/150‑1 (MQ=255)

                                                                                                                            aagaagATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGa                         >  1:4517335/1‑150 (MQ=255)

                                                                                                                              gaagaTATGGAAGCGCGCCACTAACAGTTAACCAAATGCGGCACAATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGAGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCTAACAACGCCCGTAGTGGTACTCCATGACt                       <  2:4682896/150‑1 (MQ=255)

                                                                                                                                agaTATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGc                     >  2:53663/1‑150 (MQ=255)

                                                                                                                                 gaTATGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCg                    <  1:5061356/150‑1 (MQ=255)

                                                                                                                                  atatGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCgg                   >  2:464518/1‑150 (MQ=255)

                                                                                                                                     tGGAAGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAAATGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAg                >  1:5297392/1‑150 (MQ=255)

                                                                                                                                        aaGCGCGCCACNAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGata             >  1:2762874/1‑150 (MQ=255)

                                                                                                                                         aGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGatat            <  2:876568/150‑1 (MQ=255)

                                                                                                                                         aGCGCGCCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGAGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATTACTGCGGAAGatat            >  1:1238958/1‑150 (MQ=255)

                                                                                                                                          gcgcgcCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGATATc           <  2:2075024/150‑1 (MQ=255)

                                                                                                                                             cgcCACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGATATCCAg        <  2:5258979/150‑1 (MQ=255)

                                                                                                                                             cgcCACGAACAGTTAACCAACGGCGGCACCATCCTCTGTTTAAATTGAGCGTCTGGCTACCAACCAGGCGCCAACCACACGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGATATCCAg        <  1:550438/150‑1 (MQ=255)

                                                                                                                                               ccACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGATATCCAGCa      >  2:4608816/1‑150 (MQ=255)

                                                                                                                                                cACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGATATCCAGCAt     >  2:4042162/1‑150 (MQ=255)

                                                                                                                                                cACTAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGATATCCAGCAt     >  2:3192620/1‑150 (MQ=255)

                                                                                                                                                cACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGATATCCAGCAt     >  2:764706/1‑150 (MQ=255)

                                                                                                                                                  cGAACAGTTAACCAACGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGATATCCAGCATCa   <  2:4511325/150‑1 (MQ=255)

                                                                                                                                                  cGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGATATCCAGCATCa   <  2:4952847/150‑1 (MQ=255)

GTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGATCGCCCATCACGGTCGCAAGCATAAAGAAGATATGGAAGCGCGCCACGAACAGTTAACCAAAGGCGGCACCATCCTCTGATTAAAATGAGCGTCTGGCTACCAACCAGGCGCCAACCACAAGTAAAATTGCGCCACAAATTGGGCCGAACAACGCCCGTAGTGGTACGCCATGACTGCGGAAGATATCCAGCATCAG  >  CP009273/1511959‑1512255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`