Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,526,607 G→A 100% D179N (GAT→AAT)  ydcC → H repeat‑associated putative transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,526,6070GA99.1% 528.8 / ‑2.8 227D179N (GAT→AAT) ydcCH repeat‑associated putative transposase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (122/102);  total (123/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 228 total aligned reads displayed.

ATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGTTACGCAATGAGTGAA  >  CP009273/1526516‑1526753
                                                                                           |                                                                                                                                                  
aTCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGaaa                                                                                          >  2:186874/1‑150 (MQ=11)
aTCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGaaa                                                                                          >  1:777422/1‑150 (MQ=11)
 tCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAAc                                                                                         <  2:3578943/150‑1 (MQ=14)
 tCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAAc                                                                                         <  2:2178066/150‑1 (MQ=14)
 tCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAAc                                                                                         <  2:311732/150‑1 (MQ=14)
  cTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAAcc                                                                                        <  2:3729716/150‑1 (MQ=17)
   tAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCa                                                                                       <  1:3891601/150‑1 (MQ=17)
   tAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCa                                                                                       >  2:2772970/1‑150 (MQ=17)
    aaTGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAg                                                                                      <  1:4022488/150‑1 (MQ=17)
    aaTGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAg                                                                                      <  1:2618998/150‑1 (MQ=17)
     aTGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAgg                                                                                     <  2:3886077/150‑1 (MQ=17)
     aTGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAgg                                                                                     >  1:794773/1‑150 (MQ=17)
      tGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAggg                                                                                    <  2:2498099/150‑1 (MQ=17)
      tGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAggg                                                                                    <  1:2506567/150‑1 (MQ=17)
      tGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAggg                                                                                    <  2:1427661/150‑1 (MQ=17)
        ataTTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGggc                                                                                  <  1:568074/148‑1 (MQ=17)
        agaTTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGggc                                                                                  >  2:4316266/1‑150 (MQ=17)
           ttACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGggcggc                                                                               <  1:350772/150‑1 (MQ=17)
            tACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCt                                                                              >  2:2212276/1‑150 (MQ=17)
             aCAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCta                                                                             >  2:1601635/1‑150 (MQ=17)
               aGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCtaaa                                                                           >  2:1169/1‑150 (MQ=17)
               aGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCtaaa                                                                           >  1:5228685/1‑150 (MQ=17)
               aGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCtaaa                                                                           >  1:1673038/1‑150 (MQ=17)
                gCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCtaaat                                                                          <  1:1557777/150‑1 (MQ=17)
                 cTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCtaaata                                                                         >  2:4476777/1‑150 (MQ=17)
                  tATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCtaaataa                                                                        <  1:2487905/150‑1 (MQ=17)
                   aTCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCtaaataaa                                                                       >  1:2640650/1‑150 (MQ=17)
                   aTCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCtaaataaa                                                                       >  1:1500576/1‑150 (MQ=17)
                   aTCCCAGAAATTCTTAACATTCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCtaaataaa                                                                       <  2:4551099/150‑1 (MQ=12)
                    tCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAg                                                                      >  1:484278/1‑150 (MQ=17)
                    tCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAg                                                                      >  1:997101/1‑150 (MQ=17)
                     cccAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGc                                                                     <  1:4157152/150‑1 (MQ=17)
                     cccAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGc                                                                     >  1:1903340/1‑150 (MQ=17)
                      ccAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGcc                                                                    >  2:3220744/1‑150 (MQ=17)
                       cAGAACTTCTTAACATGCTGGATATAAAAGGAAAAATCATCACAACAGATGCGATGTGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAAAAGGGAGGTGATAATTTATTCGAGGTAAAAGGACACAAGGGGCGGCTAAATACAGCCt                                                                   >  2:4098759/1‑150 (MQ=11)
                        aGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCtt                                                                  >  2:902598/1‑150 (MQ=17)
                           aCTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGa                                                               >  1:3977818/1‑150 (MQ=17)
                            cttcttAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAg                                                              <  1:4229719/150‑1 (MQ=17)
                             ttcttAACATGCTGGATATTAAATGAAAAATAATCACAACTGATTCGATGGGTTGCCAGAAAAATATTGCAGTGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGTAAACCAGGGGCGGCTAAATAAAGCCTTTGAgg                                                             <  2:4864566/150‑1 (MQ=11)
                                 tAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGaaaa                                                         >  2:1531116/1‑150 (MQ=17)
                                  aaCATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGaaaaa                                                        >  1:267247/1‑150 (MQ=17)
                                   aCATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAAt                                                       >  2:3402092/1‑150 (MQ=17)
                                   aCATGCTGGATATTAAAGGAAAAATAATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAAt                                                       <  1:5047421/150‑1 (MQ=17)
                                    cATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAAtt                                                      <  1:3171830/150‑1 (MQ=17)
                                     aTGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAAttt                                                     >  2:2394178/1‑150 (MQ=17)
                                     aTGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTATGAGGAAAAAttt                                                     >  2:4579088/1‑150 (MQ=17)
                                       gCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGACAAATTTcc                                                   >  2:707513/1‑150 (MQ=17)
                                       gCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTcc                                                   <  1:3670014/150‑1 (MQ=17)
                                       gCTGGAGATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTcc                                                   <  2:1142742/150‑1 (MQ=17)
                                        cTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCg                                                  <  2:1645933/150‑1 (MQ=17)
                                         tGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGc                                                 >  2:4221541/1‑150 (MQ=17)
                                          ggATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCt                                                >  2:1214868/1‑150 (MQ=17)
                                          ggATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCt                                                <  1:5067651/150‑1 (MQ=17)
                                           gATATTACAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTg                                               <  1:4986277/150‑1 (MQ=17)
                                           gATATTAAAGGAAATATCATCACAACTGAAGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAAACAGGGGCGGCTAAATAAAGACTTTGAGGAAAAATTTCCTCTg                                               >  2:2682224/1‑150 (MQ=11)
                                           gATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTg                                               >  1:1167128/1‑150 (MQ=17)
                                            atatTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGa                                              >  1:2897091/1‑150 (MQ=17)
                                            atatTAAAGGAAAAATCATCACAACTGATGAGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGa                                              <  2:1967383/150‑1 (MQ=17)
                                             tatTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGaa                                             >  1:2420188/1‑150 (MQ=17)
                                              atTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGaaa                                            >  1:2404310/1‑150 (MQ=17)
                                                  aaGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAAt                                        <  2:4054918/150‑1 (MQ=17)
                                                  aaGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAAt                                        >  1:4633254/1‑150 (MQ=17)
                                                   aGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAAtt                                       >  1:1034876/1‑150 (MQ=17)
                                                   aGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAAtt                                       <  2:65725/150‑1 (MQ=17)
                                                     gAAAAATCATCTGAACTGATGCGATGGGTTGCCAGAAATATTTTGAAGAGAAGATACAATTACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTATTTAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTaa                                     <  1:3225773/150‑1 (MQ=11)
                                                      aaaaaTCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTaaa                                    >  1:847243/1‑150 (MQ=17)
                                                      aaaaaTCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTaaa                                    >  1:97877/1‑150 (MQ=17)
                                                      aaaaaTCATAACAACTGATGAGATGGGTTACCAGAAAAATATTGCAGAGAAGATACAAAAACAGGTAGTTTATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGTCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTaaa                                    <  1:1169219/150‑1 (MQ=11)
                                                        aaaTCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAaata                                  <  2:2933973/150‑1 (MQ=17)
                                                        aaaTCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAaata                                  <  1:5218834/150‑1 (MQ=17)
                                                         aaTCATCACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAaataa                                 <  2:404830/150‑1 (MQ=17)
                                                          atcatcACAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAaataat                                <  2:4701135/150‑1 (MQ=17)
                                                           tcatcaCAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATc                               <  2:4649206/150‑1 (MQ=17)
                                                            catcaCAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAATAATTTCCGCTGAAAGAATTAAATAATcc                              <  1:3678489/150‑1 (MQ=17)
                                                            catcaCAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATcc                              >  1:1740695/1‑150 (MQ=17)
                                                            catcaCAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATcc                              >  2:3084326/1‑150 (MQ=17)
                                                            catcaCAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATcc                              >  1:860800/1‑150 (MQ=17)
                                                             atcaCAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCa                             <  1:4134261/150‑1 (MQ=17)
                                                             atcaCAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCa                             >  1:1936377/1‑150 (MQ=17)
                                                             atcaCAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCa                             <  1:4577328/150‑1 (MQ=17)
                                                              tcaCAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAg                            <  2:326281/150‑1 (MQ=17)
                                                              tcaCAACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAg                            >  2:1310555/1‑150 (MQ=17)
                                                                 caACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGACTTAAATAATCCAgcgc                         <  2:421792/150‑1 (MQ=21)
                                                                 caACTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAgcgc                         >  1:3830209/1‑150 (MQ=21)
                                                                    cTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATg                      >  2:5207351/1‑150 (MQ=21)
                                                                    cTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATg                      <  2:2924790/150‑1 (MQ=21)
                                                                    cTGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATg                      <  2:245886/150‑1 (MQ=21)
                                                                     tGATGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGa                     <  1:229865/150‑1 (MQ=21)
                                                                       aTGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACa                   <  2:2305163/150‑1 (MQ=21)
                                                                        tGCGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAg                  >  2:338100/1‑150 (MQ=21)
                                                                          cGATGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGtt                >  1:2167147/1‑150 (MQ=21)
                                                                            aTGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGTTAc              >  2:4909278/1‑150 (MQ=21)
                                                                            aTGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGTTAc              >  1:2084930/1‑150 (MQ=21)
                                                                            aTGGGTTGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGTTAc              >  1:1249180/1‑150 (MQ=21)
                                                                                 ttGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGTTACGCAAt         <  2:3987929/150‑1 (MQ=21)
                                                                                 ttGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGTTACGCAAt         >  2:2413707/1‑150 (MQ=21)
                                                                                  tGCCAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGTTACGCAATg        >  2:2030852/1‑150 (MQ=25)
                                                                                    ccAGAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGTTACGCAATGAg      <  1:2840500/150‑1 (MQ=37)
                                                                                       gAAAAATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGTTACGCAATGAGTGa   >  1:646446/1‑150 (MQ=37)
                                                                                        aaaaATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGTTACGCAATGAGTGaa  >  2:3663121/1‑150 (MQ=37)
                                                                                           |                                                                                                                                                  
ATCTAATGAGATTACAGCTATCCCAGAACTTCTTAACATGCTGGATATTAAAGGAAAAATCATCACAACTGATGCGATGGGTTGCCAGAAAGATATTGCAGAGAAGATACAAAAACAGGGAGGTGATTATTTATTCGCGGTAAAAGGAAACCAGGGGCGGCTAAATAAAGCCTTTGAGGAAAAATTTCCGCTGAAAGAATTAAATAATCCAGCGCATGACAGTTACGCAATGAGTGAA  >  CP009273/1526516‑1526753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑