Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,552,629 C→A 100% D216Y (GAT→TAT)  ddpD ← D,D‑dipeptide permease system, ATP‑binding component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,552,6290CA97.3% 1000.1 / 14.8 331D216Y (GAT→TAT) ddpDD,D‑dipeptide permease system, ATP‑binding component
Reads supporting (aligned to +/- strand):  ref base C (6/3);  new base A (182/140);  total (188/143)
Fisher's exact test for biased strand distribution p-value = 7.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 333 total aligned reads displayed.

ACTCCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAA  >  CP009273/1552481‑1552775
                                                                                                                                                    |                                                                                                                                                  
 cTCCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAAt                                                                                                                                                  >  2:1183487/1‑150 (MQ=255)
 cTCCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAAt                                                                                                                                                  <  1:702744/150‑1 (MQ=255)
 cTCCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAAt                                                                                                                                                  <  1:1900132/150‑1 (MQ=255)
  tCCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATg                                                                                                                                                 <  1:3127879/150‑1 (MQ=255)
     aTGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCt                                                                                                                                              >  2:2019833/1‑150 (MQ=255)
      tGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCtg                                                                                                                                             >  1:1356835/1‑150 (MQ=255)
       gTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCtga                                                                                                                                            >  2:2559635/1‑150 (MQ=255)
        ttCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCtgat                                                                                                                                           <  1:1980149/150‑1 (MQ=255)
         tCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCtgatg                                                                                                                                          <  2:2702156/150‑1 (MQ=255)
          cTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCtgatga                                                                                                                                         >  1:1664032/1‑150 (MQ=255)
             gTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACa                                                                                                                                      <  2:4068043/150‑1 (MQ=255)
             gTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACa                                                                                                                                      <  2:389723/150‑1 (MQ=255)
               gcgcATTGCAGCAAACCAATGGTATACTGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCATCTTTCAATTACTCTTCCGGCATACATCACGTAAACGCTATCTCACATTTGCGACACCACGGCCATATAATGGCTGATGAACAGa                                                                                                                                    <  2:826531/150‑1 (MQ=255)
                 gcATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAAc                                                                                                                                  >  2:1560098/1‑150 (MQ=255)
                  cATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAAca                                                                                                                                 >  2:1009333/1‑149 (MQ=255)
                   aTTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCg                                                                                                                                >  1:2575276/1‑150 (MQ=255)
                       cagcaAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGt                                                                                                                            >  1:2979671/1‑150 (MQ=255)
                          caAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTcc                                                                                                                         <  2:4507872/150‑1 (MQ=255)
                          caAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTcc                                                                                                                         >  1:2711143/1‑150 (MQ=255)
                          caAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTcc                                                                                                                         <  1:5070174/150‑1 (MQ=255)
                           aaaCCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCa                                                                                                                        >  1:2495601/1‑150 (MQ=255)
                             aCCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACt                                                                                                                      <  1:463336/150‑1 (MQ=255)
                              ccAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTg                                                                                                                     <  1:1456551/150‑1 (MQ=255)
                                  tGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGgcgc                                                                                                                 >  1:3204241/1‑150 (MQ=255)
                                     tataCGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCggg                                                                                                              >  2:5194760/1‑150 (MQ=255)
                                     tataCGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGAAAACGCTATCGCACAGTTGCGACACCACGGCCAAATAATGGCTGATGAACAGAACAGCAGTTCCACTGTCGCggg                                                                                                              >  2:1426335/1‑150 (MQ=255)
                                      ataCGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGc                                                                                                             >  1:2277950/1‑150 (MQ=255)
                                      ataCGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGc                                                                                                             >  2:3078252/1‑150 (MQ=255)
                                        aCGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCtt                                                                                                           <  1:3711518/150‑1 (MQ=255)
                                         cGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCttt                                                                                                          >  2:2784794/1‑150 (MQ=255)
                                         cGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCttt                                                                                                          <  1:4851050/150‑1 (MQ=255)
                                          tgATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACTCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTa                                                                                                         >  2:4744116/2‑150 (MQ=255)
                                          ggATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTa                                                                                                         >  2:3384003/1‑150 (MQ=255)
                                              gCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGtt                                                                                                     <  1:109922/150‑1 (MQ=255)
                                               ccGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGttt                                                                                                    >  1:4165001/1‑150 (MQ=255)
                                               ccGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGttt                                                                                                    <  2:3106466/150‑1 (MQ=255)
                                               ccGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGttt                                                                                                    <  2:2131045/150‑1 (MQ=255)
                                               ccGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGttt                                                                                                    <  1:4751253/150‑1 (MQ=255)
                                               ccGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGttt                                                                                                    >  1:4760533/1‑150 (MQ=255)
                                               ccGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGttt                                                                                                    >  2:5060441/1‑150 (MQ=255)
                                                cGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCGTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTa                                                                                                   >  1:60265/1‑150 (MQ=255)
                                                  gggATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAg                                                                                                 <  1:2145134/150‑1 (MQ=255)
                                                  gggATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAg                                                                                                 <  1:4927872/150‑1 (MQ=255)
                                                        atgGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACg                                                                                           <  1:4210761/150‑1 (MQ=255)
                                                          ggATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCa                                                                                         <  2:4397586/150‑1 (MQ=255)
                                                           gATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAg                                                                                        >  1:4082544/1‑150 (MQ=255)
                                                                cGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCt                                                                                   <  2:3465791/150‑1 (MQ=255)
                                                                cGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGAGCGGGCTTTATGTTTAAGCAAACGCAGTACCt                                                                                   <  1:5285152/150‑1 (MQ=255)
                                                                cGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCt                                                                                   <  1:4596151/150‑1 (MQ=255)
                                                                 gTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTg                                                                                  <  2:3219896/150‑1 (MQ=255)
                                                                   cTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCa                                                                                <  2:2919643/150‑1 (MQ=255)
                                                                     gCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAAc                                                                              >  1:748981/1‑150 (MQ=255)
                                                                      ccGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACt                                                                             <  2:3652140/150‑1 (MQ=255)
                                                                       cGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTg                                                                            >  1:4439034/1‑150 (MQ=255)
                                                                       cGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTg                                                                            >  1:3840695/1‑150 (MQ=255)
                                                                           aCGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCAcc                                                                        >  2:4768350/1‑150 (MQ=255)
                                                                              ccgcTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTg                                                                     >  1:4738042/1‑150 (MQ=255)
                                                                                gcTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGAc                                                                   <  1:4520844/150‑1 (MQ=255)
                                                                                    tCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTcc                                                               >  2:3737109/1‑150 (MQ=255)
                                                                                    tCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTcc                                                               <  1:1039397/150‑1 (MQ=255)
                                                                                      aaTTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAg                                                             <  2:2392035/150‑1 (MQ=255)
                                                                                       aTTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAgc                                                            >  2:761085/1‑150 (MQ=255)
                                                                                       aTTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAgc                                                            >  2:415377/1‑150 (MQ=255)
                                                                                          aCGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTGCCTGCAACTGCACCGTGACGTCCAGCGcc                                                         >  2:2224945/1‑150 (MQ=255)
                                                                                          aCGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGcc                                                         >  2:3588108/1‑150 (MQ=255)
                                                                                             cTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTa                                                      >  1:3644080/1‑150 (MQ=255)
                                                                                              ttCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAg                                                     <  2:4431992/150‑1 (MQ=255)
                                                                                               tCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGt                                                    <  1:1551567/150‑1 (MQ=255)
                                                                                               tCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGt                                                    >  2:3179329/1‑150 (MQ=255)
                                                                                                ccGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTc                                                   <  2:4183749/150‑1 (MQ=255)
                                                                                                ccGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTc                                                   <  1:1143269/150‑1 (MQ=255)
                                                                                                    cATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGtt                                               <  1:4982819/150‑1 (MQ=255)
                                                                                                     aTACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtc                                              <  1:2330164/150‑1 (MQ=255)
                                                                                                           cACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGc                                        <  1:3049088/150‑1 (MQ=255)
                                                                                                            aCGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCa                                       >  2:4903000/1‑150 (MQ=255)
                                                                                                             cGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCaa                                      >  2:4642458/1‑150 (MQ=255)
                                                                                                                aaaCGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCaataa                                   >  2:5141924/1‑150 (MQ=255)
                                                                                                                 aaCGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCaataat                                  >  2:1807138/1‑150 (MQ=255)
                                                                                                                      tATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAAtt                             <  2:5217724/150‑1 (MQ=255)
                                                                                                                      tATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAAtt                             <  1:797184/150‑1 (MQ=255)
                                                                                                                       aTCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTg                            >  2:1353016/1‑150 (MQ=255)
                                                                                                                        tCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATAGc                           >  2:3286862/1‑150 (MQ=255)
                                                                                                                        tCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGc                           <  2:5323421/150‑1 (MQ=255)
                                                                                                                        tCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGc                           <  2:5014873/150‑1 (MQ=255)
                                                                                                                        tCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGc                           <  1:3393923/150‑1 (MQ=255)
                                                                                                                         cGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCACTTGCg                          >  2:871347/1‑150 (MQ=255)
                                                                                                                          gCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCgg                         >  1:1283875/1‑150 (MQ=255)
                                                                                                                             caGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTc                      <  1:2352104/150‑1 (MQ=255)
                                                                                                                             caGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTc                      <  2:1864775/150‑1 (MQ=255)
                                                                                                                              aGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCg                     <  1:1196220/150‑1 (MQ=255)
                                                                                                                                  gCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAgg                 <  2:541237/150‑1 (MQ=255)
                                                                                                                                      caccacGGCCATATAATGGCTGATGAACAGAACCGCAGGTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGaa             <  1:1837832/150‑1 (MQ=255)
                                                                                                                                       accacGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAAt            <  1:2216532/150‑1 (MQ=255)
                                                                                                                                       accacGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAAt            <  1:1758084/150‑1 (MQ=255)
                                                                                                                                         cacGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGc          >  2:682012/1‑150 (MQ=255)
                                                                                                                                            ggCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAg       <  1:574473/150‑1 (MQ=255)
                                                                                                                                               cATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAgcgc    >  2:2296532/1‑150 (MQ=255)
                                                                                                                                                atatAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCa   >  1:2438040/1‑150 (MQ=255)
                                                                                                                                                atatAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCa   >  1:515955/1‑150 (MQ=255)
                                                                                                                                                atatAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGACAGCGCa   >  2:1576538/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ACTCCATGTTCTGGTGCGCATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAA  >  CP009273/1552481‑1552775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑