Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,552,648 C→A 23.7% A209A (GCG→GCT)  ddpD ← D,D‑dipeptide permease system, ATP‑binding component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,552,6480CA23.7% 829.0 / 182.8 347A209A (GCG→GCT) ddpDD,D‑dipeptide permease system, ATP‑binding component
Reads supporting (aligned to +/- strand):  ref base C (153/111);  new base A (45/38);  total (198/149)
Fisher's exact test for biased strand distribution p-value = 6.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 351 total aligned reads displayed.

CATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAATCATTACCCGCTGGCGCATACC  >  CP009273/1552499‑1552797
                                                                                                                                                     |                                                                                                                                                     
 aTTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCg                                                                                                                                                      >  2:2197210/1‑150 (MQ=255)
     cagcaAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGt                                                                                                                                                  >  1:1098402/1‑150 (MQ=255)
     cagcaAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGt                                                                                                                                                  >  1:2979671/1‑150 (MQ=255)
      agcaAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGtt                                                                                                                                                 >  2:1554931/1‑150 (MQ=255)
       gcaAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTc                                                                                                                                                >  1:4448559/1‑150 (MQ=255)
         aaaCCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCa                                                                                                                                              >  2:5306800/1‑150 (MQ=255)
         aaaCCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCa                                                                                                                                              >  1:2495601/1‑150 (MQ=255)
            ccAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTg                                                                                                                                           >  1:5014873/1‑150 (MQ=255)
              aaTGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGgc                                                                                                                                         >  1:4632639/1‑150 (MQ=255)
               aTGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGgcg                                                                                                                                        >  1:4751003/1‑150 (MQ=255)
                      aCGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCtt                                                                                                                                 <  1:3711166/150‑1 (MQ=255)
                       cGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCttt                                                                                                                                >  2:2784794/1‑150 (MQ=255)
                        ggATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTa                                                                                                                               >  2:3383886/1‑150 (MQ=255)
                             ccGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGttt                                                                                                                          >  1:1488498/1‑150 (MQ=255)
                             ccGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGttt                                                                                                                          >  1:4760242/1‑150 (MQ=255)
                             ccGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGttt                                                                                                                          <  2:3106466/150‑1 (MQ=255)
                             ccGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGttt                                                                                                                          <  1:477556/150‑1 (MQ=255)
                             ccGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCGTTATGgtt                                                                                                                          >  2:5060402/1‑150 (MQ=255)
                              cGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCGTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTa                                                                                                                         >  1:60265/1‑150 (MQ=255)
                               ggggATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTaa                                                                                                                        >  2:3807959/1‑150 (MQ=255)
                               ggggATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTaa                                                                                                                        >  2:1039397/1‑150 (MQ=255)
                                gggATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAg                                                                                                                       >  1:2320640/1‑150 (MQ=255)
                                gggATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAg                                                                                                                       <  1:793405/150‑1 (MQ=255)
                                 ggATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGc                                                                                                                      <  2:3983846/150‑1 (MQ=255)
                                    tgatgGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCaaa                                                                                                                   >  2:4710994/1‑150 (MQ=255)
                                     gatgGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAAc                                                                                                                  >  2:2379334/1‑150 (MQ=255)
                                      atgGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACg                                                                                                                 <  1:2275052/150‑1 (MQ=255)
                                      atgGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACg                                                                                                                 <  2:2907335/150‑1 (MQ=255)
                                       tgGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGc                                                                                                                >  1:3402174/1‑150 (MQ=255)
                                        ggATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCa                                                                                                               <  2:4397586/150‑1 (MQ=255)
                                        ggATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCa                                                                                                               <  2:4568628/150‑1 (MQ=255)
                                              cGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCt                                                                                                         <  2:3465791/150‑1 (MQ=255)
                                              cGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCt                                                                                                         >  2:1076607/1‑150 (MQ=255)
                                                  tGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCaa                                                                                                     <  2:3052701/150‑1 (MQ=255)
                                                    ccGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACt                                                                                                   <  2:3652140/150‑1 (MQ=255)
                                                     cGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTg                                                                                                  >  1:4439034/1‑150 (MQ=255)
                                                              gcTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGAc                                                                                         <  2:139993/150‑1 (MQ=255)
                                                               cTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACg                                                                                        >  1:2333746/1‑150 (MQ=255)
                                                               cTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACATGCAACTGCACCGTTACg                                                                                        >  1:2444692/1‑150 (MQ=255)
                                                                   cAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCa                                                                                    >  1:4196343/1‑150 (MQ=255)
                                                                   cAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCa                                                                                    <  2:245576/150‑1 (MQ=255)
                                                                   cAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCa                                                                                    >  1:3163567/1‑150 (MQ=255)
                                                                     aTTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAgc                                                                                  >  2:761085/1‑150 (MQ=255)
                                                                      ttACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAgcg                                                                                 >  2:3607740/1‑150 (MQ=255)
                                                                       tACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAgcgc                                                                                >  1:4951753/1‑150 (MQ=255)
                                                                        aCGCGTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGcc                                                                               <  1:172627/150‑1 (MQ=255)
                                                                           cTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTa                                                                            <  1:1611752/150‑1 (MQ=255)
                                                                           cTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTa                                                                            >  1:3644080/1‑150 (MQ=255)
                                                                           cTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTa                                                                            >  2:2281904/1‑150 (MQ=255)
                                                                            ttCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAg                                                                           <  2:4431992/150‑1 (MQ=255)
                                                                             tCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGt                                                                          <  1:1551567/150‑1 (MQ=255)
                                                                              ccGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTc                                                                         <  1:467591/150‑1 (MQ=255)
                                                                              ccGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTc                                                                         <  1:1143269/150‑1 (MQ=255)
                                                                                   aTACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtc                                                                    >  1:4883350/1‑150 (MQ=255)
                                                                                       aTCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtcgtcg                                                                <  2:2628236/150‑1 (MQ=255)
                                                                                       aTCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTtcgtcg                                                                >  1:735305/1‑150 (MQ=255)
                                                                                         cACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGc                                                              <  1:1978465/150‑1 (MQ=255)
                                                                                           cGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCaa                                                            >  1:2579866/1‑150 (MQ=255)
                                                                                             tAAACGCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCaata                                                          >  1:1264550/1‑150 (MQ=255)
                                                                                                  gCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTCGTCGGTTCGTCGGCAATAATCaa                                                     >  2:1327605/1‑150 (MQ=255)
                                                                                                  gCTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCaa                                                     >  2:508309/1‑150 (MQ=255)
                                                                                                   cTATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGNCAATAATCAAt                                                    <  1:2764947/150‑1 (MQ=255)
                                                                                                    tATCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAAtt                                                   <  2:5217724/150‑1 (MQ=255)
                                                                                                      tCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATAGc                                                 >  2:3286862/1‑150 (MQ=255)
                                                                                                      tCGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGc                                                 <  2:5014822/150‑1 (MQ=255)
                                                                                                       cGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCACTTGCg                                                >  2:871347/1‑150 (MQ=255)
                                                                                                       cGCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCg                                                <  2:693563/150‑1 (MQ=255)
                                                                                                        gCACAGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCgg                                               <  1:2124331/150‑1 (MQ=255)
                                                                                                           caGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTc                                            <  2:1864775/150‑1 (MQ=255)
                                                                                                            aGTTGCGACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCg                                           <  1:1196220/150‑1 (MQ=255)
                                                                                                                gCGACACCACGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAgg                                       <  2:541237/150‑1 (MQ=255)
                                                                                                                  gACACCACGGCCATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGgag                                     >  1:806111/1‑150 (MQ=255)
                                                                                                                    caccacGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGaa                                   <  1:1837688/150‑1 (MQ=255)
                                                                                                                    caccacGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGaa                                   >  2:42100/1‑150 (MQ=255)
                                                                                                                     accacGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAAt                                  <  2:476241/150‑1 (MQ=255)
                                                                                                                     accacGGCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAAt                                  <  1:2216532/150‑1 (MQ=255)
                                                                                                                          ggCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAg                             >  1:3096345/1‑150 (MQ=255)
                                                                                                                          ggCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAg                             <  1:574473/150‑1 (MQ=255)
                                                                                                                          ggCCATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAg                             >  2:417100/1‑150 (MQ=255)
                                                                                                                          ggCCATATAATGGCTGATGAACAGAACAGCAGTTCAACTGGAGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCAAATTGACGTCCAGCGCCGTAGTCTGTTCGTCGGCAATAATCAATTGCTGCTCGAAGGAGAATGcaat                             >  1:3831094/1‑147 (MQ=255)
                                                                                                                            ccATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAgcg                           >  2:5161743/1‑150 (MQ=255)
                                                                                                                            ccATATAATGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAgcg                           >  2:179261/1‑150 (MQ=255)
                                                                                                                             cATATAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAgcgc                          <  2:2113812/150‑1 (MQ=255)
                                                                                                                              atatAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCa                         >  2:321405/1‑150 (MQ=255)
                                                                                                                              atatAATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCa                         >  1:515955/1‑150 (MQ=255)
                                                                                                                                ataaTGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAAt                       >  1:1341725/4‑150 (MQ=255)
                                                                                                                                ataaTGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAAt                       >  1:1843277/4‑150 (MQ=255)
                                                                                                                                ataaTGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAAt                       >  1:4252255/4‑150 (MQ=255)
                                                                                                                                  aaTGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAATCa                     >  1:1005975/2‑150 (MQ=255)
                                                                                                                                   aTGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAATCAt                    >  1:4804935/1‑150 (MQ=255)
                                                                                                                                   aTGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAATCAt                    >  2:353498/1‑150 (MQ=255)
                                                                                                                                   aTGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAATCAt                    >  1:3292042/1‑150 (MQ=255)
                                                                                                                                    tGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAATCAtt                   <  2:60265/150‑1 (MQ=255)
                                                                                                                                    tGGCTGATGAACAGAACAGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAATCAtt                   >  1:4104821/1‑150 (MQ=255)
                                                                                                                                        tgatgaACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAATCATTAccc               <  2:2979671/150‑1 (MQ=255)
                                                                                                                                          atgaACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAATCATTACCCGc             >  1:4080997/1‑150 (MQ=255)
                                                                                                                                            gaACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAATCATTACCCGCTg           <  2:4518935/150‑1 (MQ=255)
                                                                                                                                             aaCAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAATCATTACCCGCTgg          <  1:4587385/150‑1 (MQ=255)
                                                                                                                                                aGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAATCATTACCCGCTGgcgc       >  2:3141894/1‑150 (MQ=255)
                                                                                                                                                aGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAATCATTACCCGCTGgcgc       >  1:522331/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CATTGCAGCAAACCAATGGTATACGGATGCCGGGGATGATGGATAACGTCTGCCGTCACGCCGCTTTCAATTACGCTTCCGGCATACATCACGTAAACGCTATCGCACAGTTGCGACACCACGGCCATATCATGGCTGATGAACAGAACCGCAGTTCCACTGGCGCGGGCTTTATGTTTAAGCAAACGCAGTACCTGCAACTGCACCGTGACGTCCAGCGCCGTAGTCGGTTCGTCGGCAATAATCAATTGCGGCTCGCAGGAGAATGCCAGCGCAATCATTACCCGCTGGCGCATACC  >  CP009273/1552499‑1552797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑