Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,586,871 G→T 100% intergenic (‑172/+51) hipB ← / ← yneO antitoxin of HipAB toxin‑antitoxin system/pseudogene, AidA homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,586,8710GT99.4% 1096.8 / ‑2.2 333intergenic (‑172/+51)hipB/yneOantitoxin of HipAB toxin‑antitoxin system/pseudogene, AidA homolog
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (184/146);  total (186/147)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 336 total aligned reads displayed.

CCGCTTAAGGGGATATTATAAGTTTTATCCTTTAGTGAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTGAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGCACACCGACATTCCCCCACAGGCTAAGGTTGTT  >  CP009273/1586724‑1587017
                                                                                                                                                   |                                                                                                                                                  
 cGCTTAAGGGGATATTATAAGTTTTATCCTTTAGTGAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAg                                                                                                                                                 >  1:1141696/1‑150 (MQ=255)
 cGCTTAAGGGGATATTATAAGTTTTATCCTTTAGTGAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAg                                                                                                                                                 <  2:4158991/150‑1 (MQ=255)
   cTTAAGGTGATATTATAAGTTTTATCCTTTAGTGAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTc                                                                                                                                               <  2:3541692/150‑1 (MQ=255)
    ttAAGGGGATATTATAAGTTTTATCCTTTAGTGAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCg                                                                                                                                              <  1:4738030/150‑1 (MQ=255)
     tAAGGGGATATTATAAGTTTTATCCTTTAGTGAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCgg                                                                                                                                             >  2:588962/1‑150 (MQ=255)
      aaGGGGATATTATAAGTTTTATCCTTTAGTGAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGc                                                                                                                                            >  2:437055/1‑150 (MQ=255)
           gATATTATAAGTTTTATCCTTTAGTGAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAatat                                                                                                                                       <  1:3112441/150‑1 (MQ=255)
            atatTATAAGTTTTATCCTTTAGTGAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATAtt                                                                                                                                      >  2:793511/1‑150 (MQ=255)
            atatTATAAGTTTTATCCTTTAGTGAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATAtt                                                                                                                                      <  2:2998165/150‑1 (MQ=255)
              attatAAGTTTTATCCTTTAGTGAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTaa                                                                                                                                    >  1:310968/1‑150 (MQ=255)
              attatAAGTTTTATCCTTTAGTGAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTaa                                                                                                                                    >  2:1616775/1‑150 (MQ=255)
                tataAGTTTTATCCTTTAGTGAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGc                                                                                                                                  <  1:1812457/150‑1 (MQ=255)
                 ataAGTTTTATCCTTTAGTGAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGcc                                                                                                                                 >  2:3098133/1‑150 (MQ=255)
                   aaGTTTTATCCTTTAGTGAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCACTATTAAGCCGc                                                                                                                               >  2:1356599/1‑150 (MQ=255)
                        ttATCCTTTAGTGAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGcc                                                                                                                          >  2:1088675/1‑150 (MQ=255)
                         tATCCTTTAGTGAGGATAAGTCAATCTTGAAACACATCTTCTCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCa                                                                                                                         <  2:910942/150‑1 (MQ=255)
                           tCCTTTAGTGAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCAtc                                                                                                                       >  1:3354129/1‑150 (MQ=255)
                               ttAGTGAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGa                                                                                                                   <  2:4479243/150‑1 (MQ=255)
                                tAGTGAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCTCCGTTTTAAGTCGGCAATATTAATCCGCATGCCATATCGAc                                                                                                                  >  2:1515181/1‑150 (MQ=255)
                                 aGTGAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACa                                                                                                                 >  1:3734585/1‑150 (MQ=255)
                                 aGTGAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACa                                                                                                                 >  2:2858812/1‑150 (MQ=255)
                                    gAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAATGCGTTGATGCGCCGATTTAAGTCGGCAATATTAAAACGCATTACATCTAGAAAttc                                                                                                              >  1:4924554/1‑148 (MQ=255)
                                    gAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGc                                                                                                              <  1:4471453/150‑1 (MQ=255)
                                         tAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCtt                                                                                                         >  1:3161244/1‑150 (MQ=255)
                                         tAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCtt                                                                                                         >  1:2642578/1‑150 (MQ=255)
                                         tAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCtt                                                                                                         >  2:695799/1‑150 (MQ=255)
                                           aGTCAATCTGGAAACACATCTTATCCTCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCAGTGTTAATTCGGCAATATTAAGCCGCATGCCATCTCGACAAGCGGCTtat                                                                                                       >  1:1009438/1‑150 (MQ=255)
                                           aGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTtat                                                                                                       <  2:2005065/150‑1 (MQ=255)
                                            gTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTtata                                                                                                      <  1:3986831/150‑1 (MQ=255)
                                            gTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATAGCCGCGCTCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTtata                                                                                                      <  2:1448360/150‑1 (MQ=255)
                                             tCAATCTGGACACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATAc                                                                                                     <  2:1051064/150‑1 (MQ=255)
                                             tCAATCTGGAAACACATCGTCTCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATAc                                                                                                     <  2:4103095/150‑1 (MQ=255)
                                             tCAATCTGGAAACACAACTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATCAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATAc                                                                                                     <  2:4404644/150‑1 (MQ=255)
                                              cAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACg                                                                                                    >  1:4581543/1‑150 (MQ=255)
                                                aTCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGt                                                                                                  >  2:1776387/1‑150 (MQ=255)
                                                aTCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGt                                                                                                  >  2:47539/1‑150 (MQ=255)
                                                  cTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGttt                                                                                                >  2:3015085/1‑150 (MQ=255)
                                                  cTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGttt                                                                                                >  1:127368/1‑150 (MQ=255)
                                                  cTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGttt                                                                                                >  2:2722232/1‑150 (MQ=255)
                                                     gAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTAcc                                                                                             <  1:5314278/150‑1 (MQ=255)
                                                          acaTCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCta                                                                                        <  2:3817508/150‑1 (MQ=255)
                                                           caTCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCtat                                                                                       <  1:1399126/150‑1 (MQ=255)
                                                              cTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATAtt                                                                                    <  1:2312407/150‑1 (MQ=255)
                                                              cTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATAtt                                                                                    <  1:2952139/150‑1 (MQ=255)
                                                                tATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTc                                                                                  >  1:61911/1‑150 (MQ=255)
                                                                  tCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCAc                                                                                <  1:9513/150‑1 (MQ=255)
                                                                    cGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCGTATACGGTTTACCAGCTATATATCACTc                                                                              >  2:3967183/1‑150 (MQ=255)
                                                                       tctACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCa                                                                           <  2:2108284/150‑1 (MQ=255)
                                                                            gggATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATg                                                                      >  1:766331/1‑150 (MQ=255)
                                                                            gggATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATg                                                                      <  1:500141/150‑1 (MQ=255)
                                                                             ggATAAACGCTGCGATCTCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGc                                                                     <  2:3343030/150‑1 (MQ=255)
                                                                             ggATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAATCCGCATGCCATCTCGACATGCGGCGTATACGGTTTACCAGCTATATTTCACTCCCAGCATGc                                                                     >  1:3624626/1‑150 (MQ=255)
                                                                               aTAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGccc                                                                   >  2:4807468/1‑150 (MQ=255)
                                                                               aTAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGccc                                                                   >  1:3226220/1‑150 (MQ=255)
                                                                                tAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCt                                                                  <  1:4154026/150‑1 (MQ=255)
                                                                                tAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCt                                                                  >  2:1957388/1‑150 (MQ=255)
                                                                                  aaCGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGa                                                                >  2:5050322/1‑150 (MQ=255)
                                                                                   aCGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAg                                                               <  2:2498879/150‑1 (MQ=255)
                                                                                   aCGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAg                                                               >  1:3857075/1‑150 (MQ=255)
                                                                                   aCGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAg                                                               <  1:2730276/150‑1 (MQ=255)
                                                                                    cGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGt                                                              <  1:4051103/150‑1 (MQ=255)
                                                                                      cTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTAt                                                            <  1:212528/150‑1 (MQ=255)
                                                                                      cTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTAt                                                            <  1:437055/150‑1 (MQ=255)
                                                                                      cTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGGTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTAt                                                            >  2:2829382/1‑150 (MQ=255)
                                                                                        gCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCg                                                          <  1:2713974/150‑1 (MQ=255)
                                                                                          gATAACCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTAACCAGCTATATTTCACACCCAGCATGCCCTGAGTATCGCt                                                        >  2:3612141/1‑150 (MQ=255)
                                                                                           atatCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGCGTCTCGcga                                                       >  2:5217414/1‑148 (MQ=255)
                                                                                                 gcgATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCt                                                 <  1:1112158/150‑1 (MQ=255)
                                                                                                     tCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATAGCTATAGCCTTTAt                                             <  2:892634/150‑1 (MQ=255)
                                                                                                      cgcgGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATc                                            >  1:164367/1‑150 (MQ=255)
                                                                                                      cgcgGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATc                                            <  2:4318966/150‑1 (MQ=255)
                                                                                                        cgGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGTGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCAc                                          <  2:2395334/150‑1 (MQ=255)
                                                                                                        cgGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCAc                                          <  1:4658913/150‑1 (MQ=255)
                                                                                                         ggATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATATCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCAcc                                         >  1:819055/1‑150 (MQ=255)
                                                                                                           aTAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTATGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACAAGCTAAATTTCACTCCCAGCATGACATGAGTATCGCTATAGACATTATCACCTa                                       >  2:4713798/1‑150 (MQ=255)
                                                                                                           aTAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTa                                       >  2:230732/1‑150 (MQ=255)
                                                                                                           aTAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTa                                       <  2:4664084/150‑1 (MQ=255)
                                                                                                             aaaaaGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAATTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGt                                     >  2:5106801/1‑150 (MQ=255)
                                                                                                              aaaaGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGtt                                    <  2:2542346/150‑1 (MQ=255)
                                                                                                              aaaaGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGtt                                    >  1:2351991/1‑150 (MQ=255)
                                                                                                                aaGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGc                                  >  1:3982206/1‑150 (MQ=255)
                                                                                                                    aggaTTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGCacac                              <  2:2661067/150‑1 (MQ=255)
                                                                                                                       aTTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGCACACCGa                           <  2:1141696/150‑1 (MQ=255)
                                                                                                                       aTTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGCACACCGa                           >  1:3885461/1‑150 (MQ=255)
                                                                                                                       aTTTAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGCACACCGa                           >  1:1991839/1‑150 (MQ=255)
                                                                                                                          tAAATAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGCACACCGACAt                        >  2:4964965/1‑150 (MQ=255)
                                                                                                                            aaTAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGCACACCGACATTc                      <  1:3407168/150‑1 (MQ=255)
                                                                                                                              tAAGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGCACACCGACATTccc                    >  1:1977374/1‑150 (MQ=255)
                                                                                                                                aGGCGTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGCACACCGACATTccccc                  <  1:1527464/150‑1 (MQ=255)
                                                                                                                                    gTTGATGCGCCGTTTTAAGTCGGCAATATTAAGCCCCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGCACACCGACATTCCCCCACAg              >  1:2447266/1‑150 (MQ=255)
                                                                                                                                      tGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGCACACCGACATTCCCCCACAGGc            >  1:3018680/1‑150 (MQ=255)
                                                                                                                                      tGATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGCACACCGACATTCCCCCACAGGc            <  1:3814775/150‑1 (MQ=255)
                                                                                                                                       gATGCGCCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGCACACCGACATTCCCCCACAGGCt           <  2:3868154/150‑1 (MQ=255)
                                                                                                                                          gcgcCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATAGCTATAGCCTTTATCACCTAGTTGCACACCGACATTCCCCCACAGGCTAAg        <  1:1616775/150‑1 (MQ=255)
                                                                                                                                          gcgcCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTGTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATAGCTATAGCCTTTATCACCTAGTTGCACACCGACATTCCCCCACAGGCTAAg        <  1:4807388/150‑1 (MQ=255)
                                                                                                                                           cgcCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGCACACCGACATTCCCCCACAGGCTAAgg       <  1:883405/150‑1 (MQ=255)
                                                                                                                                            gcCGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGCACACCGACATTCCCCCACAGGCTAAGgt      >  1:1727101/1‑150 (MQ=255)
                                                                                                                                              cGTTTTAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGCACACCGACATTCCCCCACAGGCTAAGgttg    >  1:5179089/1‑150 (MQ=255)
                                                                                                                                                ttttAAGTCGGCAATATTAAGCCGCATGCCATCTCGACCTGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCGTTCTCACCTAGTTGAACACCGACATTCCCCCACAGGCTAAGgttgtt  >  2:2958206/1‑150 (MQ=255)
                                                                                                                                                ttttAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATAGCTATAGCCTTTATCACCTAGTTGCACACCGACATTCCCCCACAGGCTAAGgttgtt  <  2:3206702/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCGCTTAAGGGGATATTATAAGTTTTATCCTTTAGTGAGGATAAGTCAATCTGGAAACACATCTTATCCGCTCTACGGGATAAACGCTGCGATATCCGCGATCGCGGATAAAAAGGAGGATTTAAATAAGGCGTTGATGCGCCGTTTGAAGTCGGCAATATTAAGCCGCATGCCATCTCGACATGCGGCTTATACGGTTTACCAGCTATATTTCACTCCCAGCATGCCCTGAGTATCGCTATAGCCTTTATCACCTAGTTGCACACCGACATTCCCCCACAGGCTAAGGTTGTT  >  CP009273/1586724‑1587017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑