Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,615,694 C→A 14.6% R36S (CGC→AGC)  ydeE → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,615,6940CA14.6% 1043.2 / 100.4 352R36S (CGC→AGC) ydeEputative transporter
Reads supporting (aligned to +/- strand):  ref base C (133/166);  new base A (18/34);  total (152/200)
Fisher's exact test for biased strand distribution p-value = 2.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 358 total aligned reads displayed.

ATCTCTAAAACAAAACATAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGC  >  CP009273/1615545‑1615843
                                                                                                                                                     |                                                                                                                                                     
aTCTCTAAAACAAAACATAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTc                                                                                                                                                       >  1:4660170/1‑150 (MQ=255)
aTCTCTAAAACAAAACATAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCAAAGGAAGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTc                                                                                                                                                       >  2:5021628/1‑150 (MQ=255)
  ctctAAAACAAAACATAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCCACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCAACGGACGCGGCGCTACGCTGCCAGTTATTACCATTTAATTGAGTCGc                                                                                                                                                     >  1:4531661/1‑150 (MQ=255)
     tAAAACAAAACATAACGAAACGCACTGCCGGACAGACAAATGAACTTATCACTACGACGCTCTACAAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTAAGCTGCCATTTATGACAATTTACTTGAGTAGCCAg                                                                                                                                                  >  2:1663028/1‑150 (MQ=255)
        aacaaaaccTAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTAc                                                                                                                                               <  2:5290332/150‑1 (MQ=255)
        aacaaaacaTAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCAGTTATGACCATTTACTTGAGTCGCCAGTAc                                                                                                                                               >  1:2638754/1‑150 (MQ=255)
         acaaaacaTAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTTAGTCGCCAGTACa                                                                                                                                              >  1:2319087/1‑150 (MQ=255)
         acaaaacaTAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACa                                                                                                                                              >  2:3069487/1‑150 (MQ=255)
         acaaaacaTAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACa                                                                                                                                              >  1:5059032/1‑150 (MQ=255)
          caaaacaTAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTAGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCAAAGCAGCCATTTATGACCATTTAATTTAGTCGCCATTACAg                                                                                                                                             >  1:1077675/1‑150 (MQ=255)
            aaacaTAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGcc                                                                                                                                           >  2:4590959/1‑150 (MQ=255)
             aacaTAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACATCCt                                                                                                                                          <  2:439111/150‑1 (MQ=255)
             aacaTAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCt                                                                                                                                          >  2:2606163/1‑150 (MQ=255)
             aacaTAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTAAGCTGCCATTTATGTCCATTTACTTGAGTAGCCCGTACAGcca                                                                                                                                          >  1:2413000/1‑149 (MQ=255)
              acaTAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTg                                                                                                                                         >  2:2649585/1‑150 (MQ=255)
                  aaCGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAAACATCGGACGCGGCGCTACGCTGGCATTTATGACCATTTACTTTTGTGGCCACTACAGCCCGAttg                                                                                                                                     >  1:1822756/1‑150 (MQ=255)
                    cGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTc                                                                                                                                   <  1:4340432/150‑1 (MQ=255)
                       aaCGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGAt                                                                                                                                >  1:2813737/1‑150 (MQ=255)
                        aCGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATc                                                                                                                               <  2:2034363/150‑1 (MQ=255)
                        aCGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATc                                                                                                                               <  1:4930738/150‑1 (MQ=255)
                            aCTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAAt                                                                                                                           >  2:1607184/1‑150 (MQ=255)
                             cTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCCTCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTAGCCAGTACAGCCTGAGTGTCGATCTAATc                                                                                                                          <  2:5149189/150‑1 (MQ=255)
                             cTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATc                                                                                                                          <  2:1888254/150‑1 (MQ=255)
                               gCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTTATCgg                                                                                                                        <  1:1543736/150‑1 (MQ=255)
                                 cGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGtt                                                                                                                      >  2:1726022/1‑150 (MQ=255)
                                 cGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGtt                                                                                                                      <  1:3910352/150‑1 (MQ=255)
                                  ggACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTa                                                                                                                     <  1:4290915/150‑1 (MQ=255)
                                   gacagacaAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTAt                                                                                                                    <  1:3061424/150‑1 (MQ=255)
                                     cagacaAATGAACTTATCCCTCCGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTTTTAACCTTCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTAGCCTGTACAGCCTGAGTGTCGATCTAATCGGTTATGc                                                                                                                  <  1:2681337/150‑1 (MQ=255)
                                         caACTGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATg                                                                                                              <  1:2465668/150‑1 (MQ=255)
                                          aaaTGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGa                                                                                                             >  1:3369604/1‑150 (MQ=255)
                                           aaTGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGAc                                                                                                            <  1:4191026/150‑1 (MQ=255)
                                           aaTGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTAGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGAc                                                                                                            <  1:5178959/150‑1 (MQ=255)
                                              gAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAAt                                                                                                         >  1:1766058/1‑150 (MQ=255)
                                                aCTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCCTTTATGACCATTTACTTGAGTAGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTg                                                                                                       >  1:1011307/1‑150 (MQ=255)
                                                aCTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTAGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTg                                                                                                       <  2:4037880/150‑1 (MQ=255)
                                                  ttATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTgcg                                                                                                     >  2:4216789/1‑150 (MQ=255)
                                                    aTCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCt                                                                                                   >  1:1627031/1‑150 (MQ=255)
                                                       ccTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCAc                                                                                                >  1:2612908/1‑150 (MQ=255)
                                                       ccTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTAGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATTCGATGACAATTTCGCTCAc                                                                                                >  1:4844514/1‑150 (MQ=255)
                                                        cTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTAGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACt                                                                                               <  2:563726/150‑1 (MQ=255)
                                                         tACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTa                                                                                              <  1:423719/150‑1 (MQ=255)
                                                          acgacgCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTAt                                                                                             <  1:409667/150‑1 (MQ=255)
                                                          acgacgCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTAt                                                                                             <  1:5189254/150‑1 (MQ=255)
                                                             acgCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTgg                                                                                          <  2:5305642/150‑1 (MQ=255)
                                                              cgCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGc                                                                                         <  1:819592/150‑1 (MQ=255)
                                                               gCTCTACCAGCTCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGcg                                                                                        <  2:2353305/150‑1 (MQ=255)
                                                               gCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTAGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGcg                                                                                        >  2:2486174/1‑150 (MQ=255)
                                                                ctctACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGcgt                                                                                       >  1:2053549/1‑150 (MQ=255)
                                                                ctctACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGcgt                                                                                       <  1:3519286/150‑1 (MQ=255)
                                                                 tctACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGcgtc                                                                                      <  1:3291210/150‑1 (MQ=255)
                                                                 tctACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGcgtc                                                                                      <  1:403496/150‑1 (MQ=255)
                                                                  ctACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGTCGCGGCGCTACGCTGCCCTTTTTGACCCATTACTTTTGTCGCCCGTACAGCCTGAGTGTGGATCGAATAGGGTATTCGATGACAATTTCGCGCACTATTGGggtcg                                                                                     >  1:1382847/1‑150 (MQ=255)
                                                                  atACCAGCGCCCTTCTTTCCTCGTCGTTGTTATTAACCATCGGACGCGGCGATACGCTGCCATTTATGACCATTTACTTGAGTAGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGcgtcg                                                                                     <  1:924541/149‑1 (MQ=255)
                                                                    aCCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGtt                                                                                   <  1:1150923/150‑1 (MQ=255)
                                                                     ccAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTAGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGttt                                                                                  <  2:4973456/150‑1 (MQ=255)
                                                                        gcgcCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTa                                                                               <  1:2180813/150‑1 (MQ=255)
                                                                          gcCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACAATTGGCGTCGTTTTTAGc                                                                             >  2:892741/1‑150 (MQ=255)
                                                                             cttcttGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTc                                                                          >  2:3395473/1‑150 (MQ=255)
                                                                                cttGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGt                                                                       >  2:697195/1‑150 (MQ=255)
                                                                                   gCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTAGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGtttt                                                                    <  1:5039298/150‑1 (MQ=255)
                                                                                    ccTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTTCGCTCACTATTGGCGTCGTTTTTATCCTCGGTTTTg                                                                   >  2:3182571/1‑150 (MQ=255)
                                                                                    ccTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTg                                                                   <  2:1312501/150‑1 (MQ=255)
                                                                                        gtcgtTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGGTTTGGTAt                                                               >  1:3225951/1‑150 (MQ=255)
                                                                                                tattaACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTTTCCTCGGGTTTGGTATACAGGCgg                                                       >  1:3648607/1‑150 (MQ=255)
                                                                                                   taACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTAGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTATCCAAGGTTTTTGGATCATGGCGGAtt                                                    >  1:4721671/1‑149 (MQ=255)
                                                                                                    aaCCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATaa                                                   >  2:3079183/1‑150 (MQ=255)
                                                                                                    aaCCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTAGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATaa                                                   <  2:1106085/150‑1 (MQ=255)
                                                                                                      ccATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGt                                                 >  1:663099/1‑150 (MQ=255)
                                                                                                      ccATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTAGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGt                                                 <  2:3643764/150‑1 (MQ=255)
                                                                                                                 ggcgCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGaaa                                      <  2:1766115/150‑1 (MQ=255)
                                                                                                                  gcgcTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAAc                                     <  1:1607184/150‑1 (MQ=255)
                                                                                                                     cTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCGCGGGTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCt                                  >  2:1931632/1‑150 (MQ=255)
                                                                                                                       aCGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCtat                                <  2:5059032/150‑1 (MQ=255)
                                                                                                                         gCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTatat                              <  2:2322701/150‑1 (MQ=255)
                                                                                                                         gCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTatat                              <  2:399051/150‑1 (MQ=255)
                                                                                                                         gCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTatat                              >  2:4332263/1‑150 (MQ=255)
                                                                                                                         gCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTatat                              <  2:4531661/150‑1 (MQ=255)
                                                                                                                            gCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGtt                           <  2:2232390/150‑1 (MQ=255)
                                                                                                                                tttATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTg                       <  1:5297115/150‑1 (MQ=255)
                                                                                                                                   aTGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCa                    <  1:1987877/150‑1 (MQ=255)
                                                                                                                                   aTGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCa                    <  2:1373410/150‑1 (MQ=255)
                                                                                                                                    tGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCaa                   <  2:3512488/150‑1 (MQ=255)
                                                                                                                                      aCCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAAtt                 <  2:4725716/150‑1 (MQ=255)
                                                                                                                                        cATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTAc               <  2:1191947/150‑1 (MQ=255)
                                                                                                                                        cATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTAc               <  2:212833/150‑1 (MQ=255)
                                                                                                                                         aTTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTAcc              <  1:1710671/150‑1 (MQ=255)
                                                                                                                                         aTTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTAcc              <  1:2536284/150‑1 (MQ=255)
                                                                                                                                         aTTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGAGGACAATTGCGCTCACTATTGGCGTCGTGTTTAACATCGGGTTTGGTTTCCTGGCGGATAAGTTCGTCAAGGAAAGCTTTATGTTACTGGCAATTagc              >  2:1539626/1‑148 (MQ=255)
                                                                                                                                           ttACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGAAAAGAAACGCTATATGTTACTGGCAAATAACGc            >  1:3819205/1‑150 (MQ=255)
                                                                                                                                           ttACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGGTTTGGTATCCAGGCGGATAAGTTCGACAAGAAAAGCTATATGTTAATGGCAATTACCGc            >  1:3476507/1‑150 (MQ=255)
                                                                                                                                             aCTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGGTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTTCCGCCt          >  2:3865947/1‑150 (MQ=255)
                                                                                                                                              cTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCtt         >  2:2915388/1‑150 (MQ=255)
                                                                                                                                              cTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCtt         <  2:2704038/150‑1 (MQ=255)
                                                                                                                                               ttGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTc        <  2:3808320/150‑1 (MQ=255)
                                                                                                                                                 gAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTATCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCGATATGTTACTGGCAATTTACGCCGTCGc      >  2:2177624/1‑150 (MQ=255)
                                                                                                                                                   gTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCa    <  2:4883836/150‑1 (MQ=255)
                                                                                                                                                   gTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCa    <  1:11410/150‑1 (MQ=255)
                                                                                                                                                    tCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAg   <  2:4660170/150‑1 (MQ=255)
                                                                                                                                                     cGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATACTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCGTCGCCAGc  >  2:2408031/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCTCTAAAACAAAACATAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCTCACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGC  >  CP009273/1615545‑1615843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑