| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,680,642 | C→A | 100% | E68D (GAG→GAT) | fumC ← | fumarate hydratase (fumarase C),aerobic Class II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,680,642 | 0 | C | A | 98.5% | 1027.4 / 4.7 | 324 | E68D (GAG→GAT) | fumC | fumarate hydratase (fumarase C),aerobic Class II |
| Reads supporting (aligned to +/- strand): ref base C (4/1); new base A (171/148); total (175/149) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 326 total aligned reads displayed.
AATTCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTT > CP009273/1680496‑1680788 | aaTTCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTatct < 1:4457689/150‑1 (MQ=255) aTTCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCtt > 1:2962242/1‑150 (MQ=255) aTTCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCtt < 1:1508284/150‑1 (MQ=255) ttCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTc > 2:1588988/1‑150 (MQ=255) ttCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTc < 1:52654/150‑1 (MQ=255) cACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAg < 1:3789803/150‑1 (MQ=255) cACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAg < 1:1294708/150‑1 (MQ=255) aCTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGa < 1:3044224/150‑1 (MQ=255) tGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGaca > 2:4753991/1‑150 (MQ=255) gCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGacaac > 2:1886170/1‑150 (MQ=255) cGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCTCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGAAAACAAg > 1:2069454/1‑150 (MQ=255) ggTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGc > 2:2422374/1‑150 (MQ=255) ggTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGc > 2:5058525/1‑150 (MQ=255) ggTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGc > 1:3968624/1‑150 (MQ=255) gTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGcc < 1:2252263/150‑1 (MQ=255) gTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGcc > 1:3449684/1‑150 (MQ=255) ttAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCt < 2:298485/150‑1 (MQ=255) ttAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCt < 2:2242783/150‑1 (MQ=255) tAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTa < 2:2513127/150‑1 (MQ=255) tAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTa < 1:3287304/150‑1 (MQ=255) tAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTa > 2:2171566/1‑150 (MQ=255) aGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCTCTCGCTTTATCTTCAGACAACAAGCCTaa > 2:764175/1‑150 (MQ=255) aGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTaa > 2:3651933/1‑150 (MQ=255) aGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCt > 1:3205514/1‑150 (MQ=255) aGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCt > 2:401234/1‑150 (MQ=255) aGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCt < 1:3668884/150‑1 (MQ=255) aGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCt < 2:3471486/150‑1 (MQ=255) gCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCtt > 2:2540864/1‑150 (MQ=255) tCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCTTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTa > 2:4309706/1‑150 (MQ=255) tCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTa < 2:4504880/150‑1 (MQ=255) gttcatgttcatGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGTATTCGTCGTAATGCTTTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCTATCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAAc < 2:3540974/150‑1 (MQ=255) ttcatgttcatgTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACt > 2:678744/1‑150 (MQ=255) catgttcatgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACttt < 2:613533/150‑1 (MQ=255) gttcatgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGc > 2:4200749/1‑150 (MQ=255) gttcatgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGc > 2:4632767/1‑150 (MQ=255) tcatgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCg > 2:1724692/1‑150 (MQ=255) catgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGc > 2:1245803/1‑150 (MQ=255) tgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTg > 2:1174940/1‑150 (MQ=255) tACTTTGTGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCAc < 1:1454065/150‑1 (MQ=255) tACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCAc > 1:1912023/1‑150 (MQ=255) tACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCAc > 2:2030219/1‑150 (MQ=255) tACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCAc < 1:714387/150‑1 (MQ=255) tttGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCt > 2:3678609/1‑150 (MQ=255) cGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGt < 2:2803497/150‑1 (MQ=255) gTGCCGGAGCCAGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGtt < 1:952259/150‑1 (MQ=255) cGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAgcg < 1:5126234/150‑1 (MQ=255) ggAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAgcgc > 2:751228/1‑150 (MQ=255) ggAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAgcgc > 2:1447013/1‑150 (MQ=255) gAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGcc > 2:4949414/1‑150 (MQ=255) aGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCa < 1:874407/150‑1 (MQ=255) gTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCa > 1:4336222/1‑150 (MQ=255) tCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCAt > 1:4686210/1‑150 (MQ=255) cTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATg < 1:332733/150‑1 (MQ=255) ccAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAAt < 1:4193589/150‑1 (MQ=255) cAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATc > 2:705438/1‑150 (MQ=255) cAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATc > 1:1852571/1‑150 (MQ=255) cgATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCAGTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCa > 2:82304/2‑150 (MQ=255) gATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAg > 1:2559623/1‑150 (MQ=255) gCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAg < 1:4547893/150‑1 (MQ=255) gCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAg < 1:2512529/150‑1 (MQ=255) cAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGt > 2:5182315/1‑150 (MQ=255) aGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTg < 2:3723750/150‑1 (MQ=255) gggAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGc > 1:3484618/1‑150 (MQ=255) gggAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCAATAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGc > 2:171209/1‑150 (MQ=255) ggAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCa < 2:2847748/150‑1 (MQ=255) ggAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCa < 1:1976376/150‑1 (MQ=255) aTTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCAttt < 2:2097364/150‑1 (MQ=255) ttCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCAtttt < 2:2720318/150‑1 (MQ=255) ttCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCAtttt < 2:4463254/150‑1 (MQ=255) cgtcATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCg > 1:274094/1‑150 (MQ=255) tcATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTc > 1:3622180/1‑150 (MQ=255) tGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATg < 2:3366113/150‑1 (MQ=255) ccTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCgg > 2:5241789/1‑150 (MQ=255) ccAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAAt < 2:3803621/150‑1 (MQ=255) cAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATg > 1:1584743/1‑150 (MQ=255) aCTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTcc < 2:4976010/150‑1 (MQ=255) aCTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTcc > 2:248831/1‑150 (MQ=255) aCTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTcc > 2:4866489/1‑150 (MQ=255) ttCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCag > 2:1489150/1‑150 (MQ=255) tCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCagc > 2:3192399/1‑150 (MQ=255) tCATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCagc < 1:4898095/150‑1 (MQ=255) cATCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCagcg > 1:5156921/1‑150 (MQ=255) aTCCGCCGCCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCagcga > 1:3821491/1‑150 (MQ=255) cgccgcCTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCagcgagcg > 1:1016669/1‑150 (MQ=255) gccgccTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGt > 1:2701170/1‑150 (MQ=255) ccgccTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGtt < 2:5267750/150‑1 (MQ=255) cgccTGACGAATGGCGCTCGCTTTATCTTCAGACAACACGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTg < 2:1016244/150‑1 (MQ=255) cgccTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTg < 2:3786153/150‑1 (MQ=255) cgccTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTg > 1:1706106/1‑150 (MQ=255) cTGACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGt > 1:571671/1‑150 (MQ=255) gACGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGttt < 1:4200406/150‑1 (MQ=255) aCGAATGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTtg < 1:678744/150‑1 (MQ=255) aaTGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTgc > 1:5073884/1‑150 (MQ=255) aTGGCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTgcg > 1:484205/1‑150 (MQ=255) ggCGCTCGCTTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGcc > 1:3574619/1‑150 (MQ=255) tcgctTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCcaca < 1:1124466/150‑1 (MQ=255) cgctTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAg < 1:2297008/150‑1 (MQ=255) cgctTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGACATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAg > 1:3746400/1‑150 (MQ=255) cgctTTATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGACATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAg > 1:1074336/1‑150 (MQ=255) tttATCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCtt > 2:3934140/1‑150 (MQ=255) | AATTCACTGGCCCGGTTAGCCAGCACTTCGTTCATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTT > CP009273/1680496‑1680788 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |