Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,699,862 T→A 100% L119* (TTG→TAG)  ydgK → DUF2569 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,699,8620TA99.4% 980.5 / ‑4.5 300L119* (TTG→TAG) ydgKDUF2569 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base A (168/129);  total (168/132)
Fisher's exact test for biased strand distribution p-value = 8.41e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 300 total aligned reads displayed.

ATTACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTTT  >  CP009273/1699715‑1700008
                                                                                                                                                   |                                                                                                                                                  
  tACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAGGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTg                                                                                                                                                >  1:4665429/1‑150 (MQ=255)
  tACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAATCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTg                                                                                                                                                >  1:2309254/1‑150 (MQ=255)
  tACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTg                                                                                                                                                <  1:5020357/150‑1 (MQ=255)
  tACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTg                                                                                                                                                <  2:4019502/150‑1 (MQ=255)
   acacacTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGACAGTAGCTGt                                                                                                                                               >  2:2785290/1‑150 (MQ=255)
    cacacTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGtt                                                                                                                                              <  1:3241571/150‑1 (MQ=255)
      cacTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTa                                                                                                                                            <  1:1659949/150‑1 (MQ=255)
         tgtgGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTGATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACtt                                                                                                                                         <  2:2579472/150‑1 (MQ=255)
            ggTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTttgt                                                                                                                                      <  2:3499934/150‑1 (MQ=255)
                   cATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAAc                                                                                                                               >  1:1025859/1‑150 (MQ=255)
                   cATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCCGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAAc                                                                                                                               <  1:478766/150‑1 (MQ=255)
                    aTCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAAcc                                                                                                                              >  1:2548643/1‑150 (MQ=255)
                        ctttctttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCAt                                                                                                                          >  1:1401455/1‑150 (MQ=255)
                         tttctttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCAtt                                                                                                                         <  1:5180254/150‑1 (MQ=255)
                          ttctttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTg                                                                                                                        <  2:4885124/150‑1 (MQ=255)
                             tttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATc                                                                                                                     <  1:4373128/150‑1 (MQ=255)
                             tttAAACGTCGGCGTGGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATc                                                                                                                     >  2:3834736/1‑150 (MQ=255)
                              ttAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCg                                                                                                                    <  1:2298059/150‑1 (MQ=255)
                               tAAACGTCCGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGt                                                                                                                   >  2:3360504/1‑150 (MQ=255)
                                aaaCGTCGGCGTTGTGTTCCTAAACACTATATTATATGTCTGCTTATTTCAGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCAAAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTc                                                                                                                  >  1:1267905/1‑150 (MQ=255)
                                   cGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTcccc                                                                                                               <  2:31888/150‑1 (MQ=255)
                                       ggCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTAtt                                                                                                           <  2:24551/150‑1 (MQ=255)
                                       ggCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTAtt                                                                                                           >  1:652284/1‑150 (MQ=255)
                                          gTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCa                                                                                                        >  2:3402134/1‑150 (MQ=255)
                                                   cTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCtcgt                                                                                               >  2:49402/1‑150 (MQ=255)
                                                   cTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCtcgt                                                                                               <  2:5187261/150‑1 (MQ=255)
                                                    tAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATATACGTAATCCAGTAGCTGTTTACTTATTTTGCAACAGCATTTATCGTCACCTATTTCAAACTCtcgtc                                                                                              >  2:2634344/1‑150 (MQ=255)
                                                     aaaCACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCtcgtcg                                                                                             >  1:4618038/1‑150 (MQ=255)
                                                      aaCACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTcgc                                                                                            >  2:1319575/1‑150 (MQ=255)
                                                           tctattatCTGGCTGCTTATTTCCGTAGTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTa                                                                                       <  2:2825994/148‑1 (MQ=255)
                                                           tataTTATCTGTCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTa                                                                                       >  1:2731497/1‑150 (MQ=255)
                                                                 atCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCg                                                                                 >  1:1256099/1‑150 (MQ=255)
                                                                    tGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACg                                                                              <  1:537443/150‑1 (MQ=255)
                                                                    tGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACg                                                                              >  1:2428926/1‑150 (MQ=255)
                                                                     ggCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGt                                                                             >  1:3832403/1‑150 (MQ=255)
                                                                       ctgctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGttt                                                                           <  2:2382995/150‑1 (MQ=255)
                                                                       ctgctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGttt                                                                           >  1:1330293/1‑150 (MQ=255)
                                                                       ctgctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGttt                                                                           <  2:4618038/150‑1 (MQ=255)
                                                                         gctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTtgt                                                                         >  2:694803/1‑150 (MQ=255)
                                                                          ctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTtgtg                                                                        >  1:3966061/1‑150 (MQ=255)
                                                                          ctTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTtgtg                                                                        >  2:2039984/1‑150 (MQ=255)
                                                                           ttATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGa                                                                       >  1:96182/1‑150 (MQ=255)
                                                                           ttATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGa                                                                       >  2:4767161/1‑150 (MQ=255)
                                                                             aTTTCCGTTTTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCTTTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAAt                                                                     <  1:2676432/150‑1 (MQ=255)
                                                                             aTTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAAt                                                                     >  1:4836138/1‑150 (MQ=255)
                                                                             aTTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAAt                                                                     <  2:4693115/150‑1 (MQ=255)
                                                                             aTTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAAt                                                                     >  2:754607/1‑150 (MQ=255)
                                                                               ttCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATcc                                                                   <  2:4390115/150‑1 (MQ=255)
                                                                                 ccGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGt                                                                 >  1:8750/1‑150 (MQ=255)
                                                                                     aTTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGtaata                                                             >  1:4995436/1‑150 (MQ=255)
                                                                                     aTTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGtaata                                                             >  1:2922448/1‑150 (MQ=255)
                                                                                     aTTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGtaata                                                             >  1:3304391/1‑150 (MQ=255)
                                                                                      ttGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGtaataa                                                            >  1:1602055/1‑150 (MQ=255)
                                                                                         cTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCt                                                         >  1:1981554/1‑150 (MQ=255)
                                                                                         cTGGCAGTAAAAGCATTCGCTTTTGCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGGTTGTGAATCCGTAATAACCt                                                         >  1:1480989/1‑150 (MQ=255)
                                                                                               gTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGt                                                   <  1:2374320/150‑1 (MQ=255)
                                                                                               gTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTCACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGt                                                   >  2:50101/1‑150 (MQ=255)
                                                                                                tAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGtt                                                  >  2:3028724/1‑150 (MQ=255)
                                                                                                 aaaaGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTa                                                 <  1:427437/150‑1 (MQ=255)
                                                                                                   aaGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGGGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAAc                                               >  2:123457/1‑150 (MQ=255)
                                                                                                   aaGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAAc                                               <  2:2065686/150‑1 (MQ=255)
                                                                                                      cATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCtg                                            >  1:3430601/1‑150 (MQ=255)
                                                                                                         tCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTCTTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCtgttg                                         >  2:4238771/1‑150 (MQ=255)
                                                                                                         tCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCtgttg                                         <  1:1105861/150‑1 (MQ=255)
                                                                                                           gCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTc                                       <  2:222502/150‑1 (MQ=255)
                                                                                                           gCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTc                                       <  1:4654435/150‑1 (MQ=255)
                                                                                                           gCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGACAGTAGCTGTTTAAGTTGTTGGCAACCGAATAGATCGTCCCCTATATCAAACGCACGTCGCGGGTAAAATCGACGTTTGTGAATCCGAAATAACCTAACAGTTCACCTGATGTc                                       >  1:3544968/1‑150 (MQ=255)
                                                                                                            cTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCg                                      >  2:1918900/1‑150 (MQ=255)
                                                                                                            cTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCg                                      <  1:1788794/150‑1 (MQ=255)
                                                                                                             tttttCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGc                                     >  1:5087984/1‑150 (MQ=255)
                                                                                                              ttttCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGcc                                    <  1:3322759/150‑1 (MQ=255)
                                                                                                               tttCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCt                                   >  2:4154273/1‑150 (MQ=255)
                                                                                                                ttCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTg                                  <  1:4327528/150‑1 (MQ=255)
                                                                                                                  cACCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGct                                >  1:4461727/1‑150 (MQ=255)
                                                                                                                   aCCCGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGctc                               >  2:3784156/1‑150 (MQ=255)
                                                                                                                    cccGTGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGctct                              <  1:2533693/150‑1 (MQ=255)
                                                                                                                        tGGAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGAt                          >  1:5233505/1‑150 (MQ=255)
                                                                                                                         ggAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGT‑AAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTa                        <  1:1643994/150‑1 (MQ=255)
                                                                                                                          gAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTa                        >  1:4217403/1‑150 (MQ=255)
                                                                                                                          gAAGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTa                        <  1:1144981/150‑1 (MQ=255)
                                                                                                                           aaGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTaa                       >  2:4536941/1‑150 (MQ=255)
                                                                                                                           aaGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTaa                       >  2:4080930/1‑150 (MQ=255)
                                                                                                                            aGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGGTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAAc                      >  1:3029476/1‑150 (MQ=255)
                                                                                                                            aGATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTACAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAAc                      <  2:1810084/150‑1 (MQ=255)
                                                                                                                             gATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACg                     <  1:3071303/150‑1 (MQ=255)
                                                                                                                             gATGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACg                     >  2:5300048/1‑150 (MQ=255)
                                                                                                                              aTGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACgg                    >  2:1353158/1‑150 (MQ=255)
                                                                                                                               tGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGa                   >  2:3368089/1‑150 (MQ=255)
                                                                                                                               tGGCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGa                   >  1:752611/1‑150 (MQ=255)
                                                                                                                                ggCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGat                  <  2:917511/150‑1 (MQ=255)
                                                                                                                                ggCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGat                  >  1:3768885/1‑150 (MQ=255)
                                                                                                                                 gCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGata                 >  2:315208/1‑150 (MQ=255)
                                                                                                                                 gCATAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGata                 >  2:5126196/1‑150 (MQ=255)
                                                                                                                                   aTAGCCGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGataat               <  1:3393252/150‑1 (MQ=255)
                                                                                                                                        cGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAggcg          <  2:3955461/150‑1 (MQ=255)
                                                                                                                                        cGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAggcg          <  2:8750/150‑1 (MQ=255)
                                                                                                                                        cGTTCGCCAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAggcg          <  1:247933/150‑1 (MQ=255)
                                                                                                                                              ccAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCtttt    >  2:4326742/1‑150 (MQ=255)
                                                                                                                                              ccAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCtttt    <  1:612007/150‑1 (MQ=255)
                                                                                                                                               cAGTAGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCttttt   <  2:2548643/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATTACACACTGTGGTTGACCATCGCTTTCTTTAAACGTCGGCGTTGTGTTCCTAAACACTATATTATCTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAGCATTCGCTTTTTCACCCGTGGAAGATGGCATAGCCGTTCGCCAGTTGCTGTTTACTTTGTTGGCAACCGCATTGATCGTCCCCTATTTCAAACGCTCGTCGCGGGTAAAAGCGACGTTTGTGAATCCGTAATAACCTTACAGTTAACCTGTTGTCGCCTGCTCTGGATTAACGGATAATAGGCGGCTTTTTT  >  CP009273/1699715‑1700008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑