BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,710,635	T→A	11.3%	E282V (GAG→GTG)	tyrS ←	tyrosyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,710,635	0	T	A	11.3%	884.3 / 82.9	309	E282V (GAG→GTG)	tyrS	tyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base T (138/136); new base A (20/15); total (158/151)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

Only 100 of 324 total aligned reads displayed.

AAGAACCGCTGAACAGGCATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCC  >  CP009273/1710486‑1710783

aaGAACCGCTGAACAGGCATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGAtct                                                                                                                                                      <  2:3398778/150‑1 (MQ=255)

aaGAACCGCTGAACAGGCATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTGTTATCTTCTTCTTCCAGGGCGTTGAtct                                                                                                                                                      >  2:448408/1‑150 (MQ=255)

  gAACCGCTGAACAGGCATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATctct                                                                                                                                                    >  1:821378/1‑150 (MQ=255)

  gAACCGCTGAACAGGCATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATcact                                                                                                                                                    >  1:2468289/1‑150 (MQ=255)

    aCCGCTGAACAGGCATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTc                                                                                                                                                  >  1:2849472/1‑150 (MQ=255)

     ccGCTGAACAGGCATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCa                                                                                                                                                 <  2:4791302/150‑1 (MQ=255)

       gCTGAACAGGCATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAAt                                                                                                                                               <  1:3310013/150‑1 (MQ=255)

          gAACAGGCATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCt                                                                                                                                            <  2:4531046/150‑1 (MQ=255)

          gAACAGGCATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCt                                                                                                                                            <  1:3876199/150‑1 (MQ=255)

          gAACAGGCATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCt                                                                                                                                            <  1:1708634/150‑1 (MQ=255)

                 cATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAg                                                                                                                                     <  2:2878593/150‑1 (MQ=255)

                 cATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCACTTCAATGCTCATAAAg                                                                                                                                     <  2:4673945/150‑1 (MQ=255)

                  aTTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAgg                                                                                                                                    <  2:1195344/150‑1 (MQ=255)

                   ttCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGt                                                                                                                                   <  1:326350/150‑1 (MQ=255)

                   ttCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGt                                                                                                                                   >  2:863549/1‑150 (MQ=255)

                     cGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTga                                                                                                                                 <  1:5105618/150‑1 (MQ=255)

                     cGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCGTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTga                                                                                                                                 <  2:1739807/150‑1 (MQ=255)

                      ggTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTgaa                                                                                                                                <  2:3738226/150‑1 (MQ=255)

                      ggTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTgaa                                                                                                                                <  2:4996455/150‑1 (MQ=255)

                         aaTACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTgaagaa                                                                                                                             <  1:1084281/150‑1 (MQ=255)

                         aaTACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTAATAAAGGTgaagaa                                                                                                                             >  2:4190379/1‑150 (MQ=255)

                          aTACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAAc                                                                                                                            <  1:3072245/150‑1 (MQ=255)

                          aTACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCACTTCAATGCTCATAAAGGTGAAGAAc                                                                                                                            >  2:4748214/1‑150 (MQ=255)

                           tACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACt                                                                                                                           >  2:1068280/1‑150 (MQ=255)

                            aCGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACtt                                                                                                                          <  2:4020081/150‑1 (MQ=255)

                             cgatttGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGTGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTc                                                                                                                         <  2:1210502/147‑1 (MQ=255)

                             cGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTc                                                                                                                         <  1:2014972/150‑1 (MQ=255)

                             cGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTc                                                                                                                         <  2:2168197/150‑1 (MQ=255)

                              gTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCa                                                                                                                        >  2:3588445/1‑150 (MQ=255)

                                 ttGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGa                                                                                                                     <  1:1444490/150‑1 (MQ=255)

                                     cgccTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCCGTACATACTGGGCGCGCGGTGCTTTACCGCTGTGTTTATCTTCTTCTACCAGGGCGTTGATCTCTTCAATGCTCATCAAGGTGAAGACCTTCAGGAAGCg                                                                                                                 >  2:3157031/1‑150 (MQ=255)

                                        cTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTa                                                                                                              >  2:4326545/1‑150 (MQ=255)

                                          gTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTaaa                                                                                                            >  2:435038/1‑150 (MQ=255)

                                              aCCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTc                                                                                                        >  2:5295473/1‑150 (MQ=255)

                                                cttcttCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGACGCGGTAAACGTCgg                                                                                                      >  2:3256079/1‑150 (MQ=255)

                                                 ttcttcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCACTGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGc                                                                                                     <  2:2646702/150‑1 (MQ=255)

                                                 ttcttcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGc                                                                                                     >  2:2808854/1‑150 (MQ=255)

                                                 tacttcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCACTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGc                                                                                                     <  2:1942378/148‑1 (MQ=255)

                                                  tcttcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCa                                                                                                    >  2:4221423/1‑150 (MQ=255)

                                                   cttcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCAt                                                                                                   >  1:4846638/1‑150 (MQ=255)

                                                   cttcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCAt                                                                                                   <  2:1760476/150‑1 (MQ=255)

                                                   cttcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCAt                                                                                                   >  1:3635407/1‑150 (MQ=255)

                                                   cttcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCAt                                                                                                   >  1:203388/1‑150 (MQ=255)

                                                   cttcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCACTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCAt                                                                                                   >  2:2109237/1‑150 (MQ=255)

                                                    ttcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATc                                                                                                  >  1:4978737/1‑150 (MQ=255)

                                                     tcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATcc                                                                                                 >  2:4449490/1‑150 (MQ=255)

                                                     tcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATcc                                                                                                 <  1:2629764/150‑1 (MQ=255)

                                                     tcACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCACTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATcc                                                                                                 <  2:2371782/150‑1 (MQ=255)

                                                      cACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCg                                                                                                <  1:1839937/150‑1 (MQ=255)

                                                           tGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAgtg                                                                                           >  1:249332/1‑150 (MQ=255)

                                                            gAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAgtgt                                                                                          >  1:4214742/1‑150 (MQ=255)

                                                            gAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAgtgt                                                                                          >  2:5134742/1‑150 (MQ=255)

                                                               ccAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGa                                                                                       <  1:4034262/150‑1 (MQ=255)

                                                                cAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCTGTTCTTTACCGCTGTTTTTATCTTATTCTTCCAGGGCGTTGATCTCTTAAATTCTCATAAAGGTGAAGAACTTCTGGATGCGGTAAACGTCGGCATCCGCAGTGTTGAt                                                                                      <  1:4606929/150‑1 (MQ=255)

                                                                    cGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAg                                                                                  >  2:3461879/1‑150 (MQ=255)

                                                                      aGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGaa                                                                                >  2:697886/1‑150 (MQ=255)

                                                                           ccTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGt                                                                           <  1:76589/150‑1 (MQ=255)

                                                                                tCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAAt                                                                      <  1:2166579/150‑1 (MQ=255)

                                                                                tCCGCCAGTACATACTGGGCGCGCGGTGCTGTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAAt                                                                      <  2:1968937/150‑1 (MQ=255)

                                                                                    ccAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGt                                                                  >  1:1866094/1‑150 (MQ=255)

                                                                                      aGTACATACTGGGCGCGCGGTGCTTTACCGATGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTAc                                                                <  2:3083628/150‑1 (MQ=255)

                                                                                       gTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTTTACg                                                               >  1:968373/1‑150 (MQ=255)

                                                                                           aTACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCt                                                           >  2:57777/1‑150 (MQ=255)

                                                                                           aTACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCt                                                           >  1:3688852/1‑150 (MQ=255)

                                                                                             aCTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCACTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTgg                                                         <  1:4385306/150‑1 (MQ=255)

                                                                                              cTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCACTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGt                                                        <  1:718531/150‑1 (MQ=255)

                                                                                               tGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATATCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCCGTGTTGATCCAGAACAGGTAGAATTTGTACGGGCTGGtt                                                       >  1:2709554/1‑150 (MQ=255)

                                                                                                   cgcgcgGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTttct                                                   <  1:5280201/150‑1 (MQ=255)

                                                                                                    gcgcgGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCACTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTttctt                                                  <  1:1578396/150‑1 (MQ=255)

                                                                                                    gcgcgGTGCTTTACCGCTGTTTTTATCTTCTTATTCCAGGGCGTTGTTATCTTCAATGCTCATAAATGTGAAGAACTTCAGGAAGCGGTAAACCTCGGCATCCGCAGTGTTGTTCCAGAAATGGTAGAAATTGTACGGGCTGGTTttatt                                                  >  1:2260180/1‑150 (MQ=255)

                                                                                                      gcgGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCg                                                <  1:3021974/150‑1 (MQ=255)

                                                                                                       cgGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCgg                                               >  1:874140/1‑150 (MQ=255)

                                                                                                        ggTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGa                                              >  2:3905969/1‑150 (MQ=255)

                                                                                                        ggTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCACTTCAATGCTCATAAAGGTGAATAACATCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTAAGGGCTGGTTTTCTTCGGa                                              >  1:896277/1‑150 (MQ=255)

                                                                                                         gTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGAt                                             >  2:2301625/1‑150 (MQ=255)

                                                                                                           gCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATcc                                           >  2:2156278/1‑150 (MQ=255)

                                                                                                            cTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCa                                          >  1:689607/1‑150 (MQ=255)

                                                                                                              ttcccGCTGTTTTTATCTTCTTCTTCCCGGGCGTTTATCTCTACAATGCTCATAAATGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAAc                                        <  1:4084406/147‑1 (MQ=255)

                                                                                                              ttACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAAc                                        <  1:2237119/150‑1 (MQ=255)

                                                                                                              ttACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAAc                                        <  1:1541054/150‑1 (MQ=255)

                                                                                                                aCCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCACTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCa                                      <  1:4748214/150‑1 (MQ=255)

                                                                                                                    cTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACt                                  <  1:478156/150‑1 (MQ=255)

                                                                                                                    cTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACt                                  <  2:2849472/150‑1 (MQ=255)

                                                                                                                              cttcttcttCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCtt                        <  1:3961137/150‑1 (MQ=255)

                                                                                                                               ttcttcttcCAGGGCGTTGATCTCTTCAATGCTCATAAATGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTc                       <  2:1602444/150‑1 (MQ=255)

                                                                                                                                tcttcttcCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCa                      >  1:340905/1‑150 (MQ=255)

                                                                                                                                 cttcttcCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAg                     >  1:5211768/1‑150 (MQ=255)

                                                                                                                                  ttcttcCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGt                    <  2:5091919/150‑1 (MQ=255)

                                                                                                                                   tcttcCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGtt                   <  2:315013/150‑1 (MQ=255)

                                                                                                                                    cttcCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGttt                  >  1:59249/1‑150 (MQ=255)

                                                                                                                                     ttcCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGtttt                 <  1:1179676/150‑1 (MQ=255)

                                                                                                                                     ttcCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTACAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGtttt                 >  1:1005823/1‑150 (MQ=255)

                                                                                                                                       ccAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTAc               <  2:2968161/150‑1 (MQ=255)

                                                                                                                                         aGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCa             >  1:1245394/1‑150 (MQ=255)

                                                                                                                                              gTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAAttt        >  1:1337765/1‑150 (MQ=255)

                                                                                                                                              gTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAAttt        >  1:2367793/1‑150 (MQ=255)

                                                                                                                                                 gATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGt     >  2:542766/1‑150 (MQ=255)

                                                                                                                                                   tctcTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGc   <  2:1866094/150‑1 (MQ=255)

                                                                                                                                                   tctcTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGc   >  2:1448589/1‑150 (MQ=255)

                                                                                                                                                    ctctTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGcc  >  1:4700901/1‑150 (MQ=255)

AAGAACCGCTGAACAGGCATTCGGTAATACGTTTTGCCGCCTGTAAACCTTCTTCACCGTGAACCAGACGAGTCACCTGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCC  >  CP009273/1710486‑1710783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`