Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,783,091 G→T 100% E134* (GAG→TAG)  aroH → 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,783,0910GT99.7% 1078.6 / ‑4.6 336E134* (GAG→TAG) aroH3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tryptophan repressible
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (199/136);  total (200/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 336 total aligned reads displayed.

CGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATGAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCTGTCCGGTA  >  CP009273/1782944‑1783237
                                                                                                                                                   |                                                                                                                                                  
 ggCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGc                                                                                                                                                 <  1:2465101/150‑1 (MQ=255)
  gCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCt                                                                                                                                                >  2:3458662/1‑150 (MQ=255)
   cTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTg                                                                                                                                               >  2:790687/1‑150 (MQ=255)
      gAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGc                                                                                                                                            >  1:1652466/1‑150 (MQ=255)
       aaaTCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCg                                                                                                                                           >  1:4333598/1‑150 (MQ=255)
         aTCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTc                                                                                                                                         <  2:47257/150‑1 (MQ=255)
         aTCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTc                                                                                                                                         >  1:1336968/1‑150 (MQ=255)
         aTCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTc                                                                                                                                         >  1:1015543/1‑150 (MQ=255)
           cGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTccc                                                                                                                                       >  2:4005577/1‑150 (MQ=255)
            gTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCa                                                                                                                                      <  2:4923348/150‑1 (MQ=255)
            gTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCa                                                                                                                                      >  2:4705897/1‑150 (MQ=255)
              aaTGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAAc                                                                                                                                    <  2:2027399/150‑1 (MQ=255)
              aaTGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAAc                                                                                                                                    >  1:2893000/1‑150 (MQ=255)
               aTGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAAcc                                                                                                                                   >  2:3319334/1‑150 (MQ=255)
                 gcgcACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACcgc                                                                                                                                 <  2:5033046/150‑1 (MQ=255)
                 gcgcACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACcgc                                                                                                                                 >  2:4404915/1‑150 (MQ=255)
                     aCCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGAcc                                                                                                                             <  2:1843735/150‑1 (MQ=255)
                     aCCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGAcc                                                                                                                             >  1:1189018/1‑150 (MQ=255)
                      ccTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGATATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGATGGGAGTCCCAACCGCGACCg                                                                                                                            >  1:1203196/1‑150 (MQ=255)
                       cTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGa                                                                                                                           >  1:5003279/1‑150 (MQ=255)
                              gAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTc                                                                                                                    <  2:3014308/150‑1 (MQ=255)
                              gAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTc                                                                                                                    >  1:4410821/1‑150 (MQ=255)
                               aaaaaCCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCg                                                                                                                   >  1:343064/1‑150 (MQ=255)
                                  aaCCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGata                                                                                                                >  1:1299232/1‑150 (MQ=255)
                                   aCCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGatat                                                                                                               >  2:2421456/1‑150 (MQ=255)
                                   aCCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGatat                                                                                                               <  2:1744012/150‑1 (MQ=255)
                                   aCCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGatat                                                                                                               >  2:370329/1‑150 (MQ=255)
                                     cACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATgg                                                                                                             >  1:2304748/1‑150 (MQ=255)
                                      aCGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGt                                                                                                            <  1:4765028/150‑1 (MQ=255)
                                      aCGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGt                                                                                                            >  1:681726/1‑150 (MQ=255)
                                        gAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGa                                                                                                          >  2:722038/1‑150 (MQ=255)
                                        gAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGa                                                                                                          >  1:306169/1‑150 (MQ=255)
                                        gAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGa                                                                                                          >  2:1079902/1‑150 (MQ=255)
                                          aCTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATAGGGTGAcc                                                                                                        >  1:2391667/1‑150 (MQ=255)
                                            tgttgtCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCgg                                                                                                      >  1:4490583/1‑150 (MQ=255)
                                                     cTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTAt                                                                                             <  1:1635811/150‑1 (MQ=255)
                                                      tGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTAtt                                                                                            >  2:5261788/1‑150 (MQ=255)
                                                       ggAAAGGACTAATATCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGAGATGGTGACCGGTCAGTTTATTg                                                                                           >  2:1137886/1‑150 (MQ=255)
                                                          aaGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTg                                                                                        >  2:4875206/1‑150 (MQ=255)
                                                          aaGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGGTTATTGCTg                                                                                        >  2:1553182/1‑150 (MQ=255)
                                                           aGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGa                                                                                       <  2:3648121/150‑1 (MQ=255)
                                                            ggACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGAt                                                                                      <  1:4029273/150‑1 (MQ=255)
                                                             gACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGAtt                                                                                     <  1:1499140/150‑1 (MQ=255)
                                                             gACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGAtt                                                                                     >  2:2979101/1‑150 (MQ=255)
                                                              aCTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTCTTGCTGAttt                                                                                    >  2:3302328/1‑150 (MQ=255)
                                                              aCTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGAttt                                                                                    <  1:3146009/150‑1 (MQ=255)
                                                                tAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTaa                                                                                  >  1:3714066/1‑150 (MQ=255)
                                                                 aaTCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCACCCGCGACCGAGTTCCTCGAGATGGTGACCGGTCAGTTTATTGCTGATTTAAt                                                                                 >  2:2751527/1‑150 (MQ=255)
                                                                  aTCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATc                                                                                <  1:3458662/150‑1 (MQ=255)
                                                                  aTCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATc                                                                                >  2:4447463/1‑150 (MQ=255)
                                                                   tctcCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTCAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGAGTTAATCa                                                                               >  2:4572860/1‑150 (MQ=255)
                                                                      ccGATCCAGATTAAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGtt                                                                            <  1:907695/150‑1 (MQ=255)
                                                                       cGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTg                                                                           <  2:2261045/150‑1 (MQ=255)
                                                                       cGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTg                                                                           >  1:5928/1‑150 (MQ=255)
                                                                        gATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTgg                                                                          >  1:1795852/1‑150 (MQ=255)
                                                                         aTCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATCTGGTGACCGGTCAGTTTATTGCTGATTTAATCAGGTggg                                                                         >  2:156907/1‑150 (MQ=255)
                                                                         aTCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTggg                                                                         >  2:4088117/1‑150 (MQ=255)
                                                                          tCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTgggg                                                                        <  1:2982031/150‑1 (MQ=255)
                                                                           ccAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGTGgc                                                                       >  1:716785/1‑150 (MQ=255)
                                                                           ccAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGgc                                                                       >  1:3542506/1‑150 (MQ=255)
                                                                           ccAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGgc                                                                       >  2:2670621/1‑150 (MQ=255)
                                                                             aGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGgcgc                                                                     >  2:1099111/1‑150 (MQ=255)
                                                                              gATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGcgcg                                                                    >  2:520380/1‑150 (MQ=255)
                                                                               aTTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGa                                                                   >  2:2189106/1‑150 (MQ=255)
                                                                                 ttAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGAtt                                                                 <  1:3587705/150‑1 (MQ=255)
                                                                                   aaaCGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTgg                                                               >  2:2487810/1‑150 (MQ=255)
                                                                                        gaaGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAAATACTTTTAAAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGAGATGGTGACCGGACAGTTAATTGCTGATTTAATCAGTTTGGGCGCTATTGGCGCAc                                                          >  2:2389062/3‑150 (MQ=255)
                                                                                            cTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGtac                                                      >  2:595607/1‑150 (MQ=255)
                                                                                             tATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGtact                                                     <  1:819932/150‑1 (MQ=255)
                                                                                             tATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGtact                                                     >  1:4870691/1‑150 (MQ=255)
                                                                                             tATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATGGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGtact                                                     >  1:3223073/1‑150 (MQ=255)
                                                                                              aTCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGtacta                                                    >  1:208402/1‑150 (MQ=255)
                                                                                               tCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGTTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGtactac                                                   <  2:1268892/150‑1 (MQ=255)
                                                                                                     aaaTCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAg                                             <  2:2888810/150‑1 (MQ=255)
                                                                                                       aTCAAGGTCTGGAGATGGAGATCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAAACGCGACCGAGTTCATCGATATGGTGAACGGTCATTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCTCAAGTAATACAGAAAGTc                                           <  2:213004/150‑1 (MQ=255)
                                                                                                          aCGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGa                                        <  2:244057/150‑1 (MQ=255)
                                                                                                          aCGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGa                                        >  1:5107120/1‑150 (MQ=255)
                                                                                                            ggTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATc                                      <  2:828362/150‑1 (MQ=255)
                                                                                                            ggTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATc                                      >  1:2012256/1‑150 (MQ=255)
                                                                                                                tGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCAcc                                  <  1:3073076/150‑1 (MQ=255)
                                                                                                                 ggAGCTGGCGCGCAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACcg                                 >  2:3217079/1‑150 (MQ=255)
                                                                                                                         cgcgcAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATgg                         >  2:5141719/1‑150 (MQ=255)
                                                                                                                            gcAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCtt                      <  1:937437/150‑1 (MQ=255)
                                                                                                                            gcAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCtt                      <  2:1652466/150‑1 (MQ=255)
                                                                                                                             cAACTTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTc                     <  2:1442303/150‑1 (MQ=255)
                                                                                                                             cAAATTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTc                     >  2:4551406/1‑150 (MQ=255)
                                                                                                                              aaaTTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCg                    >  2:1366257/1‑150 (MQ=255)
                                                                                                                              aaaTTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCg                    >  2:4233401/1‑150 (MQ=255)
                                                                                                                               aaTTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCgg                   <  1:4095905/150‑1 (MQ=255)
                                                                                                                               aaTTACTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCgg                   >  1:1889159/1‑150 (MQ=255)
                                                                                                                                   aCTTTTACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCAct               >  1:4231021/1‑150 (MQ=255)
                                                                                                                                       ttACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTcc           <  2:1015543/150‑1 (MQ=255)
                                                                                                                                       ttACAGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTcc           >  1:2592779/1‑150 (MQ=255)
                                                                                                                                           aGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCTgcc       <  2:3817283/150‑3 (MQ=255)
                                                                                                                                           aGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCTGTc       <  1:3319334/150‑1 (MQ=255)
                                                                                                                                           aGGTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCTGTc       >  2:691592/1‑150 (MQ=255)
                                                                                                                                            ggTTAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGTTTGGCGCACGTACTACCGAAAGTCTGATCCTCCGCGAAATGGCTTCGGCTCTCTCCTGTcc      <  1:50697/150‑1 (MQ=255)
                                                                                                                                            ggTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCTGTcc      <  2:1788164/150‑1 (MQ=255)
                                                                                                                                            ggTAAATTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCTGTcc      <  2:2347144/150‑1 (MQ=255)
                                                                                                                                               aaaTTAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCTGTCCGGt   >  2:4334315/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTTGTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCACGGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATGAGCTGGGCGTCCCAACCGCGACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCGATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCTGTCCGGTA  >  CP009273/1782944‑1783237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑