Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,784,871 C→A 23.7% D211Y (GAT→TAT)  ydiU ← UPF0061 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,784,8710CA23.7% 726.6 / 171.6 312D211Y (GAT→TAT) ydiUUPF0061 family protein
Reads supporting (aligned to +/- strand):  ref base C (111/127);  new base A (45/29);  total (156/156)
Fisher's exact test for biased strand distribution p-value = 4.54e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

Only 100 of 342 total aligned reads displayed.

AAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCG  >  CP009273/1784722‑1785020
                                                                                                                                                     |                                                                                                                                                     
aaaCGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCAGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCtcatc                                                                                                                                                       >  2:2833451/1‑150 (MQ=255)
  aCGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTTGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTcatcat                                                                                                                                                     <  1:1838367/150‑1 (MQ=255)
  aCGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTcatcat                                                                                                                                                     <  1:381524/150‑1 (MQ=255)
   cGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCatcatc                                                                                                                                                    >  2:3826615/1‑150 (MQ=255)
    ggCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCt                                                                                                                                                   <  2:4436220/150‑1 (MQ=255)
       ccGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCa                                                                                                                                                >  2:1524590/1‑150 (MQ=255)
         gTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAg                                                                                                                                              >  2:4512740/1‑150 (MQ=255)
            aTCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGAtg                                                                                                                                           <  2:1899268/150‑1 (MQ=255)
                aGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGAc                                                                                                                                       >  1:3408676/1‑150 (MQ=255)
                aGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGAc                                                                                                                                       <  1:4292559/150‑1 (MQ=255)
                 gCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGAcc                                                                                                                                      <  1:1019125/150‑1 (MQ=255)
                  cGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCa                                                                                                                                     <  1:2896828/150‑1 (MQ=255)
                  cGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCa                                                                                                                                     <  1:917727/150‑1 (MQ=255)
                     cAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTa                                                                                                                                  <  1:1922778/150‑1 (MQ=255)
                     cAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTa                                                                                                                                  >  2:5284003/1‑150 (MQ=255)
                      aGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTaa                                                                                                                                 >  2:4860592/1‑150 (MQ=255)
                       gCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAAt                                                                                                                                <  1:1739578/150‑1 (MQ=255)
                        ccccAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTTTTTGTCCTCATCATCTGCAAGATGTGACCAGTAATg                                                                                                                               <  2:1461035/150‑1 (MQ=255)
                        ccccAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATg                                                                                                                               <  2:196397/150‑1 (MQ=255)
                          ccAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGAc                                                                                                                             <  1:3931701/150‑1 (MQ=255)
                            agcagcGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGa                                                                                                                           >  2:212346/1‑150 (MQ=255)
                            agcagcGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGa                                                                                                                           >  1:3661183/1‑150 (MQ=255)
                            agcagcGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGa                                                                                                                           >  2:958143/1‑150 (MQ=255)
                             gcagcGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCTTTGGGCTTTTAACGACGCGGTACGTGCTACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGaa                                                                                                                          <  2:116846/150‑1 (MQ=255)
                              cagcGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAAt                                                                                                                         <  1:111927/150‑1 (MQ=255)
                              cagcGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAAt                                                                                                                         <  1:943105/150‑1 (MQ=255)
                              cagcGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAAt                                                                                                                         <  1:2269769/150‑1 (MQ=255)
                               agcGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATa                                                                                                                        <  2:3614996/150‑1 (MQ=255)
                                 cGACATGTTGTCGGTCTTCATCACCCCATGAGCAAAGCCGACCGTCTGCCAGTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGc                                                                                                                      >  2:225577/1‑150 (MQ=255)
                                     aTGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAg                                                                                                                  >  1:1647334/1‑150 (MQ=255)
                                            cGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTa                                                                                                           >  1:3598933/1‑150 (MQ=255)
                                              gTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAAc                                                                                                         <  2:1194868/150‑1 (MQ=255)
                                              gTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAAc                                                                                                         >  1:2211836/1‑150 (MQ=255)
                                                aTTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCCAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTg                                                                                                       >  2:3271249/1‑150 (MQ=255)
                                                aTTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTg                                                                                                       >  2:5171685/1‑150 (MQ=255)
                                                 ttCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGa                                                                                                      >  2:4297746/1‑150 (MQ=255)
                                                  tcatcaCCCCATGATAAAAGCCGACCGTATGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTTCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGAc                                                                                                     <  1:2993038/150‑1 (MQ=255)
                                                   catcaCCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACg                                                                                                    >  1:1546723/1‑150 (MQ=255)
                                                   catcaCCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACg                                                                                                    <  2:2750125/150‑1 (MQ=255)
                                                    atcaCCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCGTCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGa                                                                                                   >  1:4489033/1‑150 (MQ=255)
                                                     tcaCCCCATGAGCAAAGCCGCCCGTCTGCCAGTGGGCAATTACCGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGaa                                                                                                  <  2:2882888/150‑1 (MQ=255)
                                                       aCCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAAcc                                                                                                <  1:2457495/150‑1 (MQ=255)
                                                         cccATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCtt                                                                                              >  2:3091835/1‑150 (MQ=255)
                                                            aTGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTc                                                                                           <  1:1371305/150‑1 (MQ=255)
                                                                  aaaGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTc                                                                                     >  2:495233/1‑150 (MQ=255)
                                                                   aaGCCGACCGTCTGCCATTGGGCAATTAACGACGAGGTACGTGCGACAAAATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTcg                                                                                    >  1:2190693/1‑150 (MQ=255)
                                                                    aGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTcgc                                                                                   <  2:2817783/150‑1 (MQ=255)
                                                                       cGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCgcggc                                                                                >  1:140576/1‑150 (MQ=255)
                                                                        gACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCgcggcg                                                                               <  2:1179848/150‑1 (MQ=255)
                                                                        gACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCgcggcg                                                                               >  2:3966986/1‑150 (MQ=255)
                                                                         aCCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGcggcgg                                                                              <  1:3312288/150‑1 (MQ=255)
                                                                                gCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTaaa                                                                       >  2:3646275/1‑150 (MQ=255)
                                                                                   aTTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAAt                                                                    <  1:3101416/150‑1 (MQ=255)
                                                                                   aTTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAAt                                                                    <  1:610236/150‑1 (MQ=255)
                                                                                     tGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTGTCCGACTCGCGGCGGTCGTGAAAATGt                                                                  >  2:3155999/1‑150 (MQ=255)
                                                                                       ggCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTc                                                                <  1:3970614/150‑1 (MQ=255)
                                                                                       ggCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTc                                                                <  1:1190116/150‑1 (MQ=255)
                                                                                        gCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCg                                                               >  1:4022093/1‑150 (MQ=255)
                                                                                        gCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCg                                                               <  1:5168039/150‑1 (MQ=255)
                                                                                           aTTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGaaa                                                            >  1:453723/1‑150 (MQ=255)
                                                                                            ttAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAAt                                                           <  1:3247089/150‑1 (MQ=255)
                                                                                            ttAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAAt                                                           <  2:138272/150‑1 (MQ=255)
                                                                                                cgacgCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGAcc                                                       <  2:1807737/150‑1 (MQ=255)
                                                                                                 gacgCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCa                                                      >  2:4629685/1‑150 (MQ=255)
                                                                                                 gacgCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCa                                                      >  1:4885324/1‑150 (MQ=255)
                                                                                                 gacgCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCa                                                      >  2:2731346/1‑150 (MQ=255)
                                                                                                  acgCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCaa                                                     <  2:1654424/150‑1 (MQ=255)
                                                                                                    gcgGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGTGGCGGTAGTAAAAATGTTCGAAATGACCAAAg                                                   <  1:5029709/150‑1 (MQ=255)
                                                                                                    gcgGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTTAAAATGTTCGAAATGACCAAAg                                                   <  2:2507439/150‑1 (MQ=255)
                                                                                                      ggTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAgcg                                                 >  1:464318/1‑150 (MQ=255)
                                                                                                      ggTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAgcg                                                 >  1:4981292/1‑150 (MQ=255)
                                                                                                        tACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCa                                               >  1:3516396/1‑150 (MQ=255)
                                                                                                              cGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGAc                                         <  2:453723/150‑1 (MQ=255)
                                                                                                               gACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACt                                        >  1:2805412/1‑150 (MQ=255)
                                                                                                                acaacaTCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACtt                                       >  2:542194/1‑150 (MQ=255)
                                                                                                                   acaTCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGt                                    <  2:5071204/150‑1 (MQ=255)
                                                                                                                   acaTCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGt                                    <  2:3436005/150‑1 (MQ=255)
                                                                                                                    caTCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTg                                   <  2:4189849/150‑1 (MQ=255)
                                                                                                                    caTCGCTAAACCAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTg                                   >  2:2050784/1‑150 (MQ=255)
                                                                                                                      tCGCGAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGcc                                 <  2:4736323/150‑1 (MQ=255)
                                                                                                                       cGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCca                                >  2:3495551/1‑150 (MQ=255)
                                                                                                                        gCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCcac                               >  2:711970/1‑150 (MQ=255)
                                                                                                                         cTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCcaca                              >  2:787441/1‑150 (MQ=255)
                                                                                                                          tAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCacac                             >  2:2345548/1‑150 (MQ=255)
                                                                                                                           aaaCCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACg                            >  2:4372366/1‑150 (MQ=255)
                                                                                                                            aaCCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATTACGAATAGCAAAGTCATCTAACTGACGAACCTTTTACGACTCGCGGAGGTAGTAAAAATGTTCTAAATGACAAAAGAGCAGATGACTTGTTGCCacactc                           >  1:740350/1‑148 (MQ=255)
                                                                                                                             aCCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCa                          <  1:5300273/150‑1 (MQ=255)
                                                                                                                               cAGAGACGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATc                        <  2:4061588/150‑1 (MQ=255)
                                                                                                                                agagACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCa                       <  1:2120798/150‑1 (MQ=255)
                                                                                                                                 gagaCGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAg                      <  1:423757/150‑1 (MQ=255)
                                                                                                                                 gagaCGGTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAg                      >  1:2778372/1‑150 (MQ=255)
                                                                                                                                     cGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATc                  >  2:3010908/1‑150 (MQ=255)
                                                                                                                                     cGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATc                  <  1:4864305/150‑1 (MQ=255)
                                                                                                                                       gTATTTGTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATcgc                >  1:268830/1‑150 (MQ=255)
                                                                                                                                             gTCCTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTgg          >  1:3066180/1‑150 (MQ=255)
                                                                                                                                               ccTCATAATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCt        <  2:1312994/150‑1 (MQ=255)
                                                                                                                                                 tcatcaTCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTcc      <  1:2557026/150‑1 (MQ=255)
                                                                                                                                                 tcataaTCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTcc      <  1:2609159/150‑1 (MQ=255)
                                                                                                                                                  catcatCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTccg     <  1:648133/150‑1 (MQ=255)
                                                                                                                                                    tcatcTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTccgcc   <  2:681195/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAACGGCCCGTAATCAAGCGTCAGCCCCAGCAGCGACATGTTGTCGGTATTCATCACCCCATGAGCAAAGCCGACCGTCTGCCATTGGGCAATTAACGACGCGGTACGTGCGACAACATCGCTAAACCAGAGACGGTATTTGTCCTCATCATCTGCAAGATGTGACCAGTAATGACGAATAGCAAAGTCAGCTAACTGACGAACCTTTTCCGACTCGCGGCGGTAGTAAAAATGTTCGAAATGACCAAAGCGCAGATGACTTGGTGCCACACGCATCAGCATCGCGCCTGGCTCCGCCG  >  CP009273/1784722‑1785020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑