Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,820,888 C→A 100% C98F (TGC→TTC)  astE ← succinylglutamate desuccinylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,820,8880CA97.8% 871.5 / 7.9 275C98F (TGC→TTC) astEsuccinylglutamate desuccinylase
Reads supporting (aligned to +/- strand):  ref base C (4/2);  new base A (131/138);  total (135/140)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 277 total aligned reads displayed.

TGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCT  >  CP009273/1820741‑1821034
                                                                                                                                                   |                                                                                                                                                  
 gTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAAt                                                                                                                                                 <  2:2177060/150‑1 (MQ=255)
    gATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAAc                                                                                                                                              <  2:4334698/150‑1 (MQ=255)
      tCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGt                                                                                                                                            <  1:4227897/150‑1 (MQ=255)
      tCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGt                                                                                                                                            <  1:784807/150‑1 (MQ=255)
       cAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGtt                                                                                                                                           <  1:5145651/150‑1 (MQ=255)
       cAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGtt                                                                                                                                           <  2:2240390/150‑1 (MQ=255)
        aaGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGttt                                                                                                                                          <  1:3833601/150‑1 (MQ=255)
        aaGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGttt                                                                                                                                          <  1:4800304/150‑1 (MQ=255)
           gTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTccc                                                                                                                                       <  1:1315544/150‑1 (MQ=255)
           gTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTccc                                                                                                                                       <  1:4005501/150‑1 (MQ=255)
            tGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCt                                                                                                                                      <  1:236763/150‑1 (MQ=255)
            tGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCt                                                                                                                                      >  2:4027325/1‑150 (MQ=255)
            tGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCt                                                                                                                                      <  1:825547/150‑1 (MQ=255)
            tGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCt                                                                                                                                      <  2:1813274/150‑1 (MQ=255)
             gCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCtt                                                                                                                                     >  2:1163165/1‑150 (MQ=255)
             gCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGGTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCtt                                                                                                                                     <  2:2539625/150‑1 (MQ=255)
              ccAGCGCACATATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCTTTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGAAATTAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTg                                                                                                                                    <  1:1501054/150‑1 (MQ=255)
                aGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCt                                                                                                                                  <  1:2509938/150‑1 (MQ=255)
                 gcgcACAGTTTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCtt                                                                                                                                 <  1:1006285/150‑1 (MQ=255)
                 gcgcACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCtt                                                                                                                                 <  1:3489901/150‑1 (MQ=255)
                  cgcACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTc                                                                                                                                >  1:4716648/1‑150 (MQ=255)
                       aGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAgcgc                                                                                                                           >  2:3582300/1‑150 (MQ=255)
                            cTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCaggag                                                                                                                      <  2:5122110/150‑1 (MQ=255)
                                gCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACGGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGAtt                                                                                                                  <  2:1007257/150‑1 (MQ=255)
                                gCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGAtt                                                                                                                  <  2:3805785/150‑1 (MQ=255)
                                 cccTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGTTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTc                                                                                                                 <  1:659700/150‑1 (MQ=255)
                                 cccTGGACATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTc                                                                                                                 <  1:1412497/150‑1 (MQ=255)
                                  ccTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTcc                                                                                                                >  2:2783224/1‑150 (MQ=255)
                                  ccTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGAGTCATATCGCTATGGAAATAACCTTTCACTTGCTTCAGCGCAGGAGGATTcc                                                                                                                >  2:5170960/1‑150 (MQ=255)
                                     ggTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGa                                                                                                             <  2:3651425/150‑1 (MQ=255)
                                        cATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATAGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGa                                                                                                          >  1:4405809/1‑150 (MQ=255)
                                         aTAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGAt                                                                                                         >  2:1278504/1‑150 (MQ=255)
                                         aTAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGAt                                                                                                         <  2:1629613/150‑1 (MQ=255)
                                          tAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATc                                                                                                        >  1:3216115/1‑150 (MQ=255)
                                          tAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATc                                                                                                        <  1:4621854/150‑1 (MQ=255)
                                              aaTCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCa                                                                                                    >  2:4196605/1‑150 (MQ=255)
                                              aaTCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCa                                                                                                    >  1:2934056/1‑150 (MQ=255)
                                               aTCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAg                                                                                                   <  2:2144249/150‑1 (MQ=255)
                                                    ccAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAAcc                                                                                              <  2:3417439/150‑1 (MQ=255)
                                                    ccAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAAcc                                                                                              <  2:895375/150‑1 (MQ=255)
                                                    ccAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAAcc                                                                                              <  2:2895166/150‑1 (MQ=255)
                                                    ccAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGAGTCCCGAGGATCACCAGCAccc                                                                                              >  2:5236469/1‑150 (MQ=255)
                                                    ccAGGAACTGTTCCTGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGAAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAAcc                                                                                              <  1:2254342/150‑1 (MQ=255)
                                                      aGGCACTGTTCCAGTTCGCGCGCCCGACCGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGc                                                                                            <  2:2647095/150‑1 (MQ=255)
                                                      aGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGc                                                                                            >  2:4462280/1‑150 (MQ=255)
                                                         cACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCaa                                                                                         <  2:4485775/150‑1 (MQ=255)
                                                          aCTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAAc                                                                                        <  1:2411640/150‑1 (MQ=255)
                                                          aCTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAAc                                                                                        <  2:705931/150‑1 (MQ=255)
                                                          aCTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGAATCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAAc                                                                                        >  2:951481/1‑150 (MQ=255)
                                                              ttCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTaa                                                                                    >  1:540487/1‑150 (MQ=255)
                                                               tCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAAc                                                                                   <  1:2826973/150‑1 (MQ=255)
                                                               tCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAAc                                                                                   <  1:3864486/150‑1 (MQ=255)
                                                                  aGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACggg                                                                                >  1:220188/1‑150 (MQ=255)
                                                                  aGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACggg                                                                                >  1:499089/1‑150 (MQ=255)
                                                                   gTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGa                                                                               <  1:309019/150‑1 (MQ=255)
                                                                     tCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATCGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGAtc                                                                             <  1:273579/150‑1 (MQ=255)
                                                                     tCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGAtc                                                                             <  2:1421872/150‑1 (MQ=255)
                                                                       gcgcgcCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGAtctc                                                                           >  2:4620031/1‑150 (MQ=255)
                                                                        cgcgcCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCg                                                                          <  2:1462524/150‑1 (MQ=255)
                                                                           gcCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCg                                                                       >  1:5097436/1‑150 (MQ=255)
                                                                            cccGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGt                                                                      <  1:754755/150‑1 (MQ=255)
                                                                              cGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTga                                                                    <  1:1135285/150‑1 (MQ=255)
                                                                               gACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTgag                                                                   >  1:3294048/1‑150 (MQ=255)
                                                                                aCAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTgaga                                                                  >  2:568612/1‑150 (MQ=255)
                                                                                 cAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGat                                                                 <  1:4371945/150‑1 (MQ=255)
                                                                                  aGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATAGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGata                                                                >  2:4516170/1‑150 (MQ=255)
                                                                                   ggTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAACCATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGatat                                                               <  2:872906/150‑1 (MQ=255)
                                                                                    gTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATc                                                              <  2:3216115/150‑1 (MQ=255)
                                                                                     tttCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATcg                                                             <  2:2090273/150‑1 (MQ=255)
                                                                                     tttCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATcg                                                             <  1:3558406/150‑1 (MQ=255)
                                                                                       tctcCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATcgcg                                                           >  1:2348191/1‑150 (MQ=255)
                                                                                          ccGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCa                                                        >  2:1862256/1‑150 (MQ=255)
                                                                                           cGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCaa                                                       >  2:3892335/1‑150 (MQ=255)
                                                                                            gCTTTCAGCAAATCGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAg                                                      <  1:3475827/150‑1 (MQ=255)
                                                                                            gCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAg                                                      <  1:3802/150‑1 (MQ=255)
                                                                                             cTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGc                                                     >  2:670047/1‑150 (MQ=255)
                                                                                              tttCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCa                                                    <  1:2032979/150‑1 (MQ=255)
                                                                                               ttCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCaa                                                   >  2:5056337/1‑150 (MQ=255)
                                                                                                   gCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAAcgcg                                               >  2:5305941/1‑150 (MQ=255)
                                                                                                    cAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGt                                              >  2:1978585/1‑150 (MQ=255)
                                                                                                     aaaTAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTc                                             >  1:5192105/1‑150 (MQ=255)
                                                                                                        tAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAg                                          <  1:3007033/150‑1 (MQ=255)
                                                                                                        tAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGGAACGGGCTCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAg                                          >  2:3978883/1‑150 (MQ=255)
                                                                                                           cTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCAt                                       <  1:1549408/150‑1 (MQ=255)
                                                                                                             gCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCAtct                                     <  1:89844/150‑1 (MQ=255)
                                                                                                              ccAACGACCGACAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCAtctc                                    <  1:177924/150‑1 (MQ=255)
                                                                                                                        ccAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGcc                          >  1:2105014/1‑150 (MQ=255)
                                                                                                                         cAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCg                         >  2:15582/1‑150 (MQ=255)
                                                                                                                            aCATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGtct                      <  2:2348191/150‑1 (MQ=255)
                                                                                                                             cATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGtctc                     >  1:2634664/1‑150 (MQ=255)
                                                                                                                              aTTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCa                    >  1:4254047/1‑150 (MQ=255)
                                                                                                                              aTTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCa                    >  2:2018782/1‑150 (MQ=255)
                                                                                                                                 cGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTa                 >  2:3463654/1‑150 (MQ=255)
                                                                                                                                 cGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTa                 >  2:2326762/1‑150 (MQ=255)
                                                                                                                                  gATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTAc                >  1:2477484/1‑150 (MQ=255)
                                                                                                                                   aTTCATATCGCTATGGAAATATCGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTAcc               <  2:443625/150‑1 (MQ=255)
                                                                                                                                       atatCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCgtgt           >  1:1613569/1‑150 (MQ=255)
                                                                                                                                         atCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTAt         <  1:2499622/150‑1 (MQ=255)
                                                                                                                                              tATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCg    <  1:4298359/150‑1 (MQ=255)
                                                                                                                                                tGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCt  <  2:73711/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGTAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCCGCT  >  CP009273/1820741‑1821034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑