Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,821,696 A→T 100% L274Q (CTG→CAG)  astB ← succinylarginine dihydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,821,6960AT98.9% 870.2 / ‑0.9 272L274Q (CTG→CAG) astBsuccinylarginine dihydrolase
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base T (131/138);  total (132/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 272 total aligned reads displayed.

TGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTG  >  CP009273/1821549‑1821841
                                                                                                                                                   |                                                                                                                                                 
   ggCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAc                                                                                                                                              >  2:4689834/1‑150 (MQ=255)
   ggCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAAc                                                                                                                                              >  2:1781431/1‑150 (MQ=255)
      agcaCCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACtgt                                                                                                                                           >  1:5030780/1‑150 (MQ=255)
        caCCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGa                                                                                                                                         >  1:1380650/1‑150 (MQ=255)
          ccAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACt                                                                                                                                       <  2:266962/150‑1 (MQ=255)
          ccAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACt                                                                                                                                       <  2:1361147/150‑1 (MQ=255)
               atcatGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGgcga                                                                                                                                  <  2:3617908/150‑1 (MQ=255)
                    ggAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGaa                                                                                                                             >  1:5078325/1‑150 (MQ=255)
                     gAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAAc                                                                                                                            <  2:4232572/150‑1 (MQ=255)
                     gAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAAc                                                                                                                            <  1:2578470/150‑1 (MQ=255)
                     gAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAAc                                                                                                                            <  2:3135870/150‑1 (MQ=255)
                      aaCCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACg                                                                                                                           <  1:4763224/150‑1 (MQ=255)
                        ccATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGACCGcc                                                                                                                         >  1:3475221/1‑150 (MQ=255)
                         catcatCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCt                                                                                                                        <  2:4172010/150‑1 (MQ=255)
                          atcatcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTg                                                                                                                       >  1:1873975/1‑150 (MQ=255)
                          atcatcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTg                                                                                                                       >  1:2473250/1‑150 (MQ=255)
                          atcatcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTg                                                                                                                       <  1:3647297/150‑1 (MQ=255)
                           tcatcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGt                                                                                                                      <  2:4847312/150‑1 (MQ=255)
                            catcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGtt                                                                                                                     <  2:4911372/150‑1 (MQ=255)
                             atcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTtg                                                                                                                    >  1:2675426/1‑150 (MQ=255)
                             atcGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTtg                                                                                                                    <  2:1961108/150‑1 (MQ=255)
                                gcgGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCAATGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTtggtg                                                                                                                 <  1:1565636/150‑1 (MQ=255)
                                    cTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCaa                                                                                                             >  1:1120874/1‑150 (MQ=255)
                                    cTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCaa                                                                                                             <  1:1252262/150‑1 (MQ=255)
                                       agcagTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAAc                                                                                                          >  2:2132572/1‑150 (MQ=255)
                                         cagTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAg                                                                                                        >  1:3120024/1‑150 (MQ=255)
                                           gTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCa                                                                                                      >  1:4387755/1‑150 (MQ=255)
                                            ttGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCAc                                                                                                     <  2:1530566/150‑1 (MQ=255)
                                            ttGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCAc                                                                                                     <  1:4659939/150‑1 (MQ=255)
                                            ttGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCAc                                                                                                     <  2:512684/150‑1 (MQ=255)
                                             tGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCAcc                                                                                                    <  2:4022262/150‑1 (MQ=255)
                                             tGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCAcc                                                                                                    >  1:4178095/1‑150 (MQ=255)
                                              ggCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCt                                                                                                   >  1:1609065/1‑150 (MQ=255)
                                              ggCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCt                                                                                                   >  2:3009615/1‑150 (MQ=255)
                                              ggCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCt                                                                                                   <  1:2364266/150‑1 (MQ=255)
                                              ggCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCt                                                                                                   <  2:4195203/150‑1 (MQ=255)
                                               gCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTg                                                                                                  <  2:3581306/150‑1 (MQ=255)
                                                cTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTgg                                                                                                 <  1:3586557/150‑1 (MQ=255)
                                                    tAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGt                                                                                             >  1:5101330/1‑150 (MQ=255)
                                                     aaaCAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGtt                                                                                            <  2:1306684/150‑1 (MQ=255)
                                                     aaaCAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGtt                                                                                            <  1:822012/150‑1 (MQ=255)
                                                        cAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACt                                                                                         <  1:1326295/150‑1 (MQ=255)
                                                         aGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTc                                                                                        >  2:4515668/1‑150 (MQ=255)
                                                           aTAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCAc                                                                                      <  2:4791032/150‑1 (MQ=255)
                                                           aTAGGTAGACACCATATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCAc                                                                                      <  1:3836657/150‑1 (MQ=255)
                                                             aGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACgg                                                                                    <  2:1074584/150‑1 (MQ=255)
                                                               gTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGTGGTTACTCACGGCa                                                                                  <  2:2825874/150‑1 (MQ=255)
                                                               gTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCa                                                                                  >  2:2357483/1‑150 (MQ=255)
                                                                 aGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAAt                                                                                <  2:1255320/150‑1 (MQ=255)
                                                                 aGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAAt                                                                                <  2:1662305/150‑1 (MQ=255)
                                                                  gACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATc                                                                               <  1:4708265/150‑1 (MQ=255)
                                                                   acacCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCa                                                                              <  2:698898/150‑1 (MQ=255)
                                                                   acacCGTATCAGACACGGAAACCTGAGGTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCa                                                                              <  1:459822/150‑1 (MQ=255)
                                                                       cGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTc                                                                          <  2:2559662/150‑1 (MQ=255)
                                                                          aTCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCAtt                                                                       >  2:4088713/1‑150 (MQ=255)
                                                                          aTCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCAtt                                                                       >  1:2933183/1‑150 (MQ=255)
                                                                          aTCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCAtt                                                                       <  1:2057513/150‑1 (MQ=255)
                                                                                   ggAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAAc                                                              <  2:2472880/150‑1 (MQ=255)
                                                                                   ggAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAAc                                                              >  1:720722/1‑150 (MQ=255)
                                                                                   ggAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAAc                                                              <  1:1845164/150‑1 (MQ=255)
                                                                                   ggAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAAc                                                              >  1:2164992/1‑150 (MQ=255)
                                                                                   ggAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAAc                                                              <  1:1004047/150‑1 (MQ=255)
                                                                                   ggAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCATGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAAc                                                              >  1:3635331/1‑150 (MQ=255)
                                                                                     aaaCCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGc                                                            >  1:4211103/1‑150 (MQ=255)
                                                                                         cTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTg                                                        <  1:95768/150‑1 (MQ=255)
                                                                                          tGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTgg                                                       >  2:3871157/1‑150 (MQ=255)
                                                                                          tGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTgg                                                       >  1:3304880/1‑150 (MQ=255)
                                                                                           gAGTTGCCGGAACTTCTATCGCCATAAAACCTTTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCTAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGt                                                      <  1:1214611/150‑1 (MQ=255)
                                                                                            aGTTGACGGAAATTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTc                                                     >  2:1013933/1‑150 (MQ=255)
                                                                                                gCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATa                                                 <  2:2926146/150‑1 (MQ=255)
                                                                                                gCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATa                                                 <  1:2221635/150‑1 (MQ=255)
                                                                                                gCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATa                                                 <  2:4320118/150‑1 (MQ=255)
                                                                                                 ccGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATaa                                                >  1:1998988/1‑150 (MQ=255)
                                                                                                  cgtaaattCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAAc                                               <  1:4077546/144‑1 (MQ=255)
                                                                                                   ggAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACg                                              >  1:3406696/1‑150 (MQ=255)
                                                                                                    gAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGt                                             >  2:3973286/1‑150 (MQ=255)
                                                                                                     aaCTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTc                                            >  1:1827522/1‑150 (MQ=255)
                                                                                                        ttCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCgg                                         <  1:4424177/150‑1 (MQ=255)
                                                                                                        taatATCGCCATAAAACCTTTGACCCGCGCACGCTGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGGGGTTACTCACGGCAATCACGACATTATGAAAAACGCCCTGGTCGATAACGTCCgg                                         <  1:1478491/147‑1 (MQ=255)
                                                                                                             tCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGtttt                                    <  2:760964/150‑1 (MQ=255)
                                                                                                             tCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGtttt                                    <  1:4789719/150‑1 (MQ=255)
                                                                                                                ccATAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCt                                 >  1:2775066/1‑150 (MQ=255)
                                                                                                                  aTAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTgg                               >  2:4403030/1‑150 (MQ=255)
                                                                                                                   tAAAACCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGGGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTggg                              <  2:1934162/150‑1 (MQ=255)
                                                                                                                       aCCATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGaa                          >  2:2233620/1‑150 (MQ=255)
                                                                                                                        ccATTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGaaa                         >  2:3068807/1‑150 (MQ=255)
                                                                                                                         cATTGACCCGCTCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGaaaa                        >  2:3843372/1‑150 (MQ=255)
                                                                                                                          aTTGCCCCGCGCACGCTGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAACAGCACCTGGCGGTTACTCACGGAAATCACTTAATTATCAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAAt                       <  1:59963/150‑1 (MQ=255)
                                                                                                                          aTTGACCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCTAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACTTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAAt                       <  1:59871/150‑1 (MQ=255)
                                                                                                                              aCCCGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCAcc                   <  2:2728923/150‑1 (MQ=255)
                                                                                                                                ccGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTg                 <  2:954291/150‑1 (MQ=255)
                                                                                                                                ccGCGCACGCAGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGAAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTg                 <  1:2982579/150‑1 (MQ=255)
                                                                                                                                   cgcacgcaGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTg              <  2:5067640/150‑1 (MQ=255)
                                                                                                                                     cacgcaGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTggg            >  2:2540506/1‑150 (MQ=255)
                                                                                                                                        gcaGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGAt         <  2:1697425/150‑1 (MQ=255)
                                                                                                                                         caGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGAtt        >  2:3252678/1‑150 (MQ=255)
                                                                                                                                         caGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGAtt        <  2:2614792/150‑1 (MQ=255)
                                                                                                                                          aGGTTTGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGGGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTc       <  2:2000089/150‑1 (MQ=255)
                                                                                                                                             tttGCCTGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCAcc    <  1:5238656/150‑1 (MQ=255)
                                                                                                                                              ttGCCTGTACGTGTGACGGGCGCGCGACCGCCTTTGGGTGCAAAACCAGCCCCGGGCGGTTACAACCGCCAATCACGACATTATTAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCt   <  1:4516537/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TGAGGCAGCACCAACATCATGGAACCATCATCGCGGCTCAGCAGTTGGCTGTTAAACAGATAGGTAGACACCGTATCAGACACGGAAACCTGAGTTGCCGGAACTTCTATCGCCATAAAACCATTGACCCGCGCACGCAGGTTTGCCAGTAACTGTGACTGGCGAGCGAACGCCTGTTGGTGGCAAAACAGCACCTGGCGGTTACTCACGGCAATCACGTCATTATGAAAAACGCCCTGGTCGATAACGTCCGGGTTTTGCTGGGCGAAAATCACCTGTTGGGGATTCACCTG  >  CP009273/1821549‑1821841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑