BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,839,854	C→A	66.8%	R455L (CGC→CTC)	topB ←	DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,839,854	0	C	A	66.8%	201.7 / 296.6	290	R455L (CGC→CTC)	topB	DNA topoisomerase III
Reads supporting (aligned to +/- strand): ref base C (47/49); new base A (75/118); total (123/167)
Fisher's exact test for biased strand distribution p-value = 1.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.34e-01

Only 100 of 298 total aligned reads displayed.

GCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAAT  >  CP009273/1839705‑1840002

gcgcgATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATcgc                                                                                                                                                      >  2:797047/1‑150 (MQ=255)

  gcgATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCgagc                                                                                                                                                    >  2:1464873/1‑150 (MQ=255)

   cgATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGct                                                                                                                                                   <  1:1009588/150‑1 (MQ=255)

   cgATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTGTTCATCGAGct                                                                                                                                                   >  1:4552049/1‑150 (MQ=255)

    gATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTATTCATAGCGctc                                                                                                                                                  >  1:3991500/1‑150 (MQ=255)

     aTCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATAGAGctct                                                                                                                                                 >  1:3416310/1‑150 (MQ=255)

       cccGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCttt                                                                                                                                               <  1:4134749/150‑1 (MQ=255)

           gTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTtgctg                                                                                                                                           >  2:5309042/1‑150 (MQ=255)

             cATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGcc                                                                                                                                         <  1:2804515/150‑1 (MQ=255)

              aTCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCt                                                                                                                                        <  1:5186883/150‑1 (MQ=255)

                  ccGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACa                                                                                                                                    <  2:889283/150‑1 (MQ=255)

                  ccGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTATTAATAGAGCTCTTTGCTGCCTAACa                                                                                                                                    >  1:702219/1‑150 (MQ=255)

                   cGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAg                                                                                                                                   >  2:1238928/1‑150 (MQ=255)

                   cGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAg                                                                                                                                   <  1:4862974/150‑1 (MQ=255)

                    gAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGc                                                                                                                                  >  2:2038480/1‑150 (MQ=255)

                            tgtTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTAATCGAGCTCTTTGCTGCCTAACAGCGTGCGccc                                                                                                                          >  1:3777445/1‑149 (MQ=255)

                              ttGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATAGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGc                                                                                                                        >  2:3758142/1‑150 (MQ=255)

                               tGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGcc                                                                                                                       <  1:4638390/150‑1 (MQ=255)

                                gCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCTACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCt                                                                                                                      <  1:4703708/150‑1 (MQ=255)

                                gCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCt                                                                                                                      <  2:110380/150‑1 (MQ=255)

                                     ggTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTc                                                                                                                 <  2:3524918/150‑1 (MQ=255)

                                      gTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCa                                                                                                                <  1:2938870/150‑1 (MQ=255)

                                        aaaaTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGc                                                                                                              <  1:1612003/150‑1 (MQ=255)

                                        aaaaTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGc                                                                                                              >  2:1690004/1‑150 (MQ=255)

                                        aaaaTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGc                                                                                                              <  2:5033398/150‑1 (MQ=255)

                                         aaaTGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCATTTCTGCCTAACAGCGTGCGCCAGCCAGCTTCAGCa                                                                                                             >  2:3636577/1‑150 (MQ=255)

                                             ggCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTAATAAAAGCCCTCTTTTCTGCCAAAAACCGTGAGCCCCCCTGCTACAGCAAGaa                                                                                                         >  2:1811698/1‑150 (MQ=255)

                                              gcgcGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGaaa                                                                                                        >  2:656469/1‑150 (MQ=255)

                                                 cggcggCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATAGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAcg                                                                                                     >  2:4780486/1‑150 (MQ=255)

                                                  ggcggcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAcgc                                                                                                    <  1:4957598/150‑1 (MQ=255)

                                                  ggcggcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAAcgc                                                                                                    <  1:2311433/150‑1 (MQ=255)

                                                     ggcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCt                                                                                                 <  2:2799201/150‑1 (MQ=255)

                                                     ggcTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTAATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAACAAAACGCGCt                                                                                                 >  2:1138961/1‑150 (MQ=255)

                                                         gggTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAg                                                                                             <  1:179027/150‑1 (MQ=255)

                                                          ggTTTGCCGCTCTACCACTTCACCTTTTTCACAAAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGTCGTGCCGTCGTTTTATTAATATCGCTCTTTTATTCCTAAAAGAGTGAGCCAGCCTGCTTAATCAAGAAAAAGAGCTTTatc                                                                                            >  1:3018482/1‑148 (MQ=255)

                                                           gTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCg                                                                                           <  2:4358178/150‑1 (MQ=255)

                                                            tttGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGa                                                                                          <  2:570878/150‑1 (MQ=255)

                                                            tttGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGa                                                                                          <  2:5181496/150‑1 (MQ=255)

                                                             ttGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTCTTCATCGAGCTCATTGCTGCCTAACAGCGTGCGCCAGCCAGCTTCAGCAAGAAAACGCGCTTTAGCGAc                                                                                         >  2:1726880/1‑150 (MQ=255)

                                                                ccGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACaaa                                                                                      <  1:555618/150‑1 (MQ=255)

                                                                 cGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTATCGACAAAt                                                                                     >  2:2887924/1‑150 (MQ=255)

                                                                 cGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAAt                                                                                     <  1:2169388/150‑1 (MQ=255)

                                                                  gCTCTACCACTTCACCTTTTTCAAACAGCAACTCATCGCCTTTCGCAACCACAGTCCGTGGCGTGCCGTCGTTTTCTTAATAGCTGTCTTTGCTGCCTAACAGCGTGGGCCCGCCTTCTTCAGCAAGGAAAAGCTCTTTTGCGAcccatt                                                                                    >  2:4289388/1‑145 (MQ=255)

                                                                      tACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGcc                                                                                <  1:834243/150‑1 (MQ=255)

                                                                      tACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGcc                                                                                >  2:4848741/1‑150 (MQ=255)

                                                                      tACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCGTTAGCGACAAATTTGcc                                                                                >  2:322513/1‑150 (MQ=255)

                                                                       aCCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTCATCGAGCTCATTGGTGTCTAACAGCGTGCGCCAGCCAGCTTCAGCAAGAAAAAGCGCATTAGCGACAAAATTGACt                                                                               >  2:1878849/1‑150 (MQ=255)

                                                                        ccACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCtt                                                                              <  1:480601/150‑1 (MQ=255)

                                                                        ccACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCtt                                                                              <  2:717987/150‑1 (MQ=255)

                                                                         cACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCttt                                                                             <  2:4548993/150‑1 (MQ=255)

                                                                          aCTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCGTTGCTGCCTAACAGCGTGCGCCAGCCAGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTTCCTTTg                                                                            >  2:5044212/1‑150 (MQ=255)

                                                                          aCTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTATTAATAGATCTCTTTTCTTCCTAAAAGCGTGCGCCCGCCAGCTTAATCAAGAAAACGCGCGTTAGCGACAAAATTTCCtttt                                                                            >  1:1758780/1‑149 (MQ=255)

                                                                                 cTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCGTCAGCAAGAAAAAGCGCTTTAGCGACAAATTTGCCTTTTGCAATGt                                                                     >  2:88994/1‑150 (MQ=255)

                                                                                  tttttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTGTTCATCGAGCGCGTTTGTGCCAAACAGCGTGCGCCCGCCGGCTTCAGCAAAAAAAACCGCGTTAGCGACAAAATTTCCTTTGGGAAAGTc                                                                    >  1:1976579/1‑150 (MQ=255)

                                                                                   ttttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTcc                                                                   <  1:5049371/150‑1 (MQ=255)

                                                                                   ttttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTcc                                                                   <  2:4184754/150‑1 (MQ=255)

                                                                                    tttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCa                                                                  <  1:4413309/150‑1 (MQ=255)

                                                                                     ttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAg                                                                 <  1:94557/150‑1 (MQ=255)

                                                                                     ttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAg                                                                 <  1:2638662/150‑1 (MQ=255)

                                                                                     ttCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTATTCATCGCGCTCTTTGCTGCCGAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAAATTGCCTTTGGCAATGTCCAg                                                                 >  2:1032936/1‑150 (MQ=255)

                                                                                      tCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGt                                                                <  1:1025409/150‑1 (MQ=255)

                                                                                        acacaGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTc                                                              <  1:2539572/150‑1 (MQ=255)

                                                                                        acacaGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTc                                                              <  2:4032013/150‑1 (MQ=255)

                                                                                          acaGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTATTCATCGCGCTCTTTTCTGCCAAACAGCGTGCGCCCGCCTGCTTAAGCAAGAAAAACCGCGTTAGCGACAAATTTGGCTTTGGCAATGTCCAGTTGGa                                                            >  1:1191668/1‑150 (MQ=255)

                                                                                             gCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATaa                                                         <  2:4423047/150‑1 (MQ=255)

                                                                                                aCTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAacac                                                      <  1:1276820/150‑1 (MQ=255)

                                                                                                aCTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAacac                                                      <  1:2556422/150‑1 (MQ=255)

                                                                                                aCTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAacac                                                      <  1:345689/150‑1 (MQ=255)

                                                                                                   cATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACt                                                   <  1:35356/150‑1 (MQ=255)

                                                                                                   cATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACt                                                   <  1:3305276/150‑1 (MQ=255)

                                                                                                    aTCGCCTTTCGCCACCACTGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACtt                                                  <  2:4836068/150‑1 (MQ=255)

                                                                                                    aTCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACtt                                                  <  1:3863392/150‑1 (MQ=255)

                                                                                                    aTCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTGTTGTTCATCGAGCTCGTTGCTGCCGAACAGCGTGCGCCCGCCGGCGTCAGCAAGAAAACGCGCGGTAGCGACAAAATTGCCGTTGGGAATGTCCCGGTCGAGAacacacgt                                                  >  1:432409/1‑148 (MQ=255)

                                                                                                     tCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTg                                                 <  1:2399542/150‑1 (MQ=255)

                                                                                                      cGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGc                                                <  1:184479/150‑1 (MQ=255)

                                                                                                      cGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGc                                                >  2:677314/1‑150 (MQ=255)

                                                                                                         cTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGa                                             >  1:3559697/1‑150 (MQ=255)

                                                                                                         cTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTAATAGCGCTCTTTGCTGCCGAACAGCGTGGGCCAGCCGGCTTCAGCAAGAAAACGCGCGTTTTCGACAAATTTTCCTTTGGCCAAGTCCAGTTCGATAAAACACATGCGGa                                             >  2:4933780/1‑150 (MQ=255)

                                                                                                            tCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTGTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAaca                                          <  2:4001800/150‑1 (MQ=255)

                                                                                                              gCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACAcc                                        <  2:246505/150‑1 (MQ=255)

                                                                                                                caccacAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGc                                      <  1:1904828/150‑1 (MQ=255)

                                                                                                                     caGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCg                                 <  1:4765843/150‑1 (MQ=255)

                                                                                                                      aGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCgg                                <  2:1365252/150‑1 (MQ=255)

                                                                                                                      aGGCAGTGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCgg                                >  2:3881565/1‑150 (MQ=255)

                                                                                                                            tGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGGCTAACAGCGTGCGCCAGCCTGCTTAAGCAAGAAAACGCGCGTTAGCGACAAATTTTCCTTTGGCAATTTCCAGTTCGATAACACACTTTCGGAACACCGCATCCGGGCAGaa                          >  2:2140454/1‑150 (MQ=255)

                                                                                                                            tGGCGTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTGTGGCAATGTCCCGTTCGATAACACACATGCGGAACACCGCATCCGGGCAGaa                          >  2:3952664/1‑150 (MQ=255)

                                                                                                                             ggCGTGCCGTCGTGTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAAt                         >  2:3301588/1‑150 (MQ=255)

                                                                                                                              gCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAAtt                        <  1:1690012/150‑1 (MQ=255)

                                                                                                                                gTGCCGTCGTTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGc                      <  2:4582516/150‑1 (MQ=255)

                                                                                                                                      tcgtTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGTATCAGa                <  2:3825505/150‑1 (MQ=255)

                                                                                                                                      tcgtTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGTACCAGa                <  2:944513/150‑1 (MQ=255)

                                                                                                                                       cgtTTTCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGAt               >  1:1679521/1‑150 (MQ=255)

                                                                                                                                         ttttCTTCATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATAc             <  1:2083471/150‑1 (MQ=255)

                                                                                                                                          tttCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACt            <  2:2225322/150‑1 (MQ=255)

                                                                                                                                           ttcttcATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTg           <  2:3416310/150‑1 (MQ=255)

                                                                                                                                            tcttcATCGCGCTCATTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGa          <  2:2395666/150‑1 (MQ=255)

                                                                                                                                            tcttcATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGa          <  1:4369844/150‑1 (MQ=255)

                                                                                                                                            tcttcATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGa          <  2:1286093/150‑1 (MQ=255)

                                                                                                                                              ttcATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACg        <  1:2968116/150‑1 (MQ=255)

                                                                                                                                               tcATCGAGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACgg       >  1:1477243/1‑150 (MQ=255)

GCGCGATCCCGGTCATCGCCGAAAGCAGTGTTGCATCGGTAAAATGGCGCGGCGGCTGGGTTTGCCGCTCTACCACTTCACCTTTTTCACACAGCAACTCATCGCCTTTCGCCACCACAGGCAGTGGCGTGCCGTCGTTTTCTTCATCGCGCTCTTTGCTGCCTAACAGCGTGCGCCAGCCTGCTTCAGCAAGAAAACGCGCTTTAGCGACAAATTTGCCTTTGGCAATGTCCAGTTCGATAACACACTTGCGGAACACCGCATCCGGGCAGAATTGCATCAGATACTGACGGGCAAT  >  CP009273/1839705‑1840002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`