Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,872,783 C→A 23.7% S271* (TCG→TAG)  yeaP → diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,872,7830CA23.7% 889.4 / 208.1 376S271* (TCG→TAG) yeaPdiguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base C (157/130);  new base A (48/41);  total (205/171)
Fisher's exact test for biased strand distribution p-value = 9.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

Only 100 of 387 total aligned reads displayed.

TCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCG  >  CP009273/1872634‑1872931
                                                                                                                                                     |                                                                                                                                                    
  gTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGc                                                                                                                                                    >  1:2368849/1‑150 (MQ=255)
   ttttGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCt                                                                                                                                                   <  2:4330334/150‑1 (MQ=255)
    tttGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTAGCTg                                                                                                                                                  <  2:3884045/150‑1 (MQ=255)
     ttGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGc                                                                                                                                                 >  2:2894030/1‑150 (MQ=255)
       ggTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCga                                                                                                                                               >  1:3515568/1‑150 (MQ=255)
          cATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTAGCTGCGAGaa                                                                                                                                            >  2:4904270/1‑150 (MQ=255)
           ataataGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAAc                                                                                                                                           <  2:2157785/150‑1 (MQ=255)
           ataataGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAAc                                                                                                                                           >  2:1584634/1‑150 (MQ=255)
            taataGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACg                                                                                                                                          >  1:774514/1‑150 (MQ=255)
             aataGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGc                                                                                                                                         >  1:5307230/1‑150 (MQ=255)
              ataGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCa                                                                                                                                        >  2:4014464/1‑150 (MQ=255)
              ataGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCa                                                                                                                                        >  2:5116615/1‑150 (MQ=255)
               taGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCATCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCTCTGCGAGAACGCAt                                                                                                                                       <  1:1129533/150‑1 (MQ=255)
                aGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGATTTTTTAGTTGTTTCACTAAACAACGAGAATTCGGATATTTCGTAGCTTCGAGAACGCAtt                                                                                                                                      >  1:3748068/1‑150 (MQ=255)
                aGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTAGCTGCGAGAACGCAtt                                                                                                                                      <  1:4923830/150‑1 (MQ=255)
                 gTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTc                                                                                                                                     <  1:4590468/150‑1 (MQ=255)
                  tGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTca                                                                                                                                    <  2:683291/150‑1 (MQ=255)
                   ggCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTcag                                                                                                                                   >  2:5269420/1‑150 (MQ=255)
                     cGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTcagca                                                                                                                                 <  2:1880775/150‑1 (MQ=255)
                     cGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTAGCTGCGAGAACGCATTcagca                                                                                                                                 <  1:2577424/150‑1 (MQ=255)
                      gATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTcagcag                                                                                                                                <  1:3356341/150‑1 (MQ=255)
                       aTCTGTTACTCATTCAGGTTGGCGAGCGCCATAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCagcagc                                                                                                                               >  1:1732264/1‑150 (MQ=255)
                          tGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCaaa                                                                                                                            <  2:3512939/150‑1 (MQ=255)
                          tGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTAGCTGCGAGAACGCATTCAGCAGCaaa                                                                                                                            >  1:3772293/1‑150 (MQ=255)
                           gTTTCTCATTCAGTTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAAt                                                                                                                           <  1:4531164/150‑1 (MQ=255)
                           gTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAAt                                                                                                                           >  2:1958591/1‑150 (MQ=255)
                           gTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAAt                                                                                                                           >  2:3316535/1‑150 (MQ=255)
                           gTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTAGCTGCGAGAACGCATTCAGCAGCAAAt                                                                                                                           <  1:3870199/150‑1 (MQ=255)
                             ttCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCTGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATAc                                                                                                                         <  2:1100747/150‑1 (MQ=255)
                             ttCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATAc                                                                                                                         <  2:4587361/150‑1 (MQ=255)
                             ttCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTAGCTGCGAGAACGCATTCAGCAGCAAATAc                                                                                                                         <  1:2205263/150‑1 (MQ=255)
                                tcATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTg                                                                                                                      <  2:3790968/150‑1 (MQ=255)
                                 cATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTgg                                                                                                                     >  2:356585/1‑150 (MQ=255)
                                 cATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTgg                                                                                                                     <  2:4094440/150‑1 (MQ=255)
                                   ttCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTAGCTGCGAGAACGCATTCAGCAGCAAATACGTGgag                                                                                                                   >  2:2194860/1‑150 (MQ=255)
                                     cAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGaa                                                                                                                 >  2:4887142/1‑150 (MQ=255)
                                      aGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAat                                                                                                                <  1:813982/150‑1 (MQ=255)
                                      aGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAat                                                                                                                <  1:121214/150‑1 (MQ=255)
                                      aGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGGTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAat                                                                                                                >  2:1335462/1‑150 (MQ=255)
                                       ggTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAata                                                                                                               >  1:1791046/1‑150 (MQ=255)
                                        gTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAatat                                                                                                              <  2:2863231/150‑1 (MQ=255)
                                        gTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAatat                                                                                                              >  1:1597102/1‑150 (MQ=255)
                                           ggCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCAc                                                                                                           >  2:2668242/1‑150 (MQ=255)
                                            gagAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACt                                                                                                          <  1:3306562/148‑1 (MQ=255)
                                            gCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACt                                                                                                          <  1:360959/150‑1 (MQ=255)
                                             cGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTAGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACtt                                                                                                         <  1:1415821/150‑1 (MQ=255)
                                               aGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAg                                                                                                       <  2:3162524/150‑1 (MQ=255)
                                                       aaTACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGtt                                                                                               <  1:1905335/150‑1 (MQ=255)
                                                       aaTACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGtt                                                                                               <  2:4813380/150‑1 (MQ=255)
                                                            gCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTAGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGAttt                                                                                          <  1:3992174/150‑1 (MQ=255)
                                                               ccAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGta                                                                                       >  2:3447717/1‑150 (MQ=255)
                                                                cAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTAGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGtat                                                                                      <  1:2258201/150‑1 (MQ=255)
                                                                 aGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGtatt                                                                                     <  2:4649223/150‑1 (MQ=255)
                                                                    aaaaTGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATc                                                                                  >  2:398575/1‑150 (MQ=255)
                                                                    aaaaTGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTAGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATc                                                                                  <  2:338725/150‑1 (MQ=255)
                                                                            gAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGcc                                                                          <  2:255800/150‑1 (MQ=255)
                                                                             aaGTTAGTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCa                                                                         <  1:5079410/150‑1 (MQ=255)
                                                                                 tATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCt                                                                     >  2:2783925/1‑150 (MQ=255)
                                                                                  aTTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCtt                                                                    <  1:1480158/150‑1 (MQ=255)
                                                                                  aTTGGTAGTCTCGTCGGTGATGATTTTTTAGTTGTTTCACTAAACAACGAGAATGCTGATAATTCGTAGCTGAGAGAACGCATTCAGCAGCAAATACGATGATAATATAAATAATGTGATGTTGATTTGTATTATACAGTTGAAAGTCtt                                                                    >  2:591065/1‑150 (MQ=255)
                                                                                   ttGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTg                                                                   <  2:37702/150‑1 (MQ=255)
                                                                                   ttGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTg                                                                   <  2:4734143/150‑1 (MQ=255)
                                                                                     ggTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGc                                                                 >  2:245455/1‑150 (MQ=255)
                                                                                     ggTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTAGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGc                                                                 <  1:671798/150‑1 (MQ=255)
                                                                                      gtcgtcTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCa                                                                >  1:5036516/1‑150 (MQ=255)
                                                                                        cgtcTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCAta                                                              <  1:2894030/150‑1 (MQ=255)
                                                                                         gtcTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCAtag                                                             >  2:1507638/1‑150 (MQ=255)
                                                                                               gcggTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAg                                                       <  1:5134325/150‑1 (MQ=255)
                                                                                                cggTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGt                                                      >  1:3061521/1‑150 (MQ=255)
                                                                                                 ggTGATGAGTTTTTAGTTGTGTCTCTACACAACGAGAAGGCGGATTTTTCGTCGCTGCGATAACGCATTCAGCAGCAAATACGTGGAGATTCTCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCTtagttttagtc                                                     <  2:263395/150‑8 (MQ=255)
                                                                                                    gatgaGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGAt                                                  <  1:955322/150‑1 (MQ=255)
                                                                                                        aGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTg                                              >  1:4290792/1‑150 (MQ=255)
                                                                                                           ttttAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGaaa                                           <  1:2814795/150‑1 (MQ=255)
                                                                                                             ttAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAaaca                                         <  1:4089506/150‑1 (MQ=255)
                                                                                                               aGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAaacaac                                       <  1:4028429/150‑1 (MQ=255)
                                                                                                               aGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAaacaac                                       <  1:2647773/150‑1 (MQ=255)
                                                                                                               aGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAaacaac                                       <  2:540222/150‑1 (MQ=255)
                                                                                                                gttgttTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAAcc                                      <  2:4479185/150‑1 (MQ=255)
                                                                                                                 ttgttTCACTAAACAACGAGAATGCGGATATTTCGTAGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCg                                     <  2:808342/150‑1 (MQ=255)
                                                                                                                  tgttTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGa                                    >  1:525192/1‑150 (MQ=255)
                                                                                                                  tgttTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGa                                    >  1:1340052/1‑150 (MQ=255)
                                                                                                                     ttCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGc                                 >  1:1308986/1‑150 (MQ=255)
                                                                                                                     ttCAATAAACAAAGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGTATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATTGTAGAAGTCGATCCTGAAACAACCGATGc                                 <  2:5033801/150‑1 (MQ=255)
                                                                                                                       cACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAg                               <  2:3977706/150‑1 (MQ=255)
                                                                                                                        aCTAAACAACGAGAATGCGGATATTTCGTAGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGa                              >  2:1059925/1‑150 (MQ=255)
                                                                                                                         cTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGAc                             <  2:1833561/150‑1 (MQ=255)
                                                                                                                         cTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGAc                             >  1:717923/1‑150 (MQ=255)
                                                                                                                             acaacGAGAATGCGGATATTTCGTAGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTg                         >  1:197186/1‑150 (MQ=255)
                                                                                                                               aacGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGcc                       >  2:206220/1‑150 (MQ=255)
                                                                                                                               aacGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGcc                       >  2:404601/1‑150 (MQ=255)
                                                                                                                                 cGAGAAGGCGGATATTTCGTAGCTGCGAGAACGCCTTCAGCAGCAAATCCGGGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGACACCACCGATGCAGACAGTGCCCt                     <  2:295453/150‑1 (MQ=255)
                                                                                                                                    gaATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCa                  >  1:3133198/1‑150 (MQ=255)
                                                                                                                                      aTGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCAtg                >  2:4279858/1‑150 (MQ=255)
                                                                                                                                        gCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCAtgct              <  1:585865/150‑1 (MQ=255)
                                                                                                                                         cGGATATTTCGTAGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTTGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCAtgctg             >  1:3914821/1‑150 (MQ=255)
                                                                                                                                           gATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGcc           >  2:4555772/1‑150 (MQ=255)
                                                                                                                                              atTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGat        >  2:71655/1‑150 (MQ=255)
                                                                                                                                                ttCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGatat      >  1:4158405/1‑150 (MQ=255)
                                                                                                                                                ttCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGatat      >  2:4146662/1‑150 (MQ=255)
                                                                                                                                                   gtcgCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGc   >  1:4829756/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCGTTTTGGTCATAATAGTGGCGATCTGTTTCTCATTCAGGTTGGCGAGCGCCTTAATACGCTCCAGCAAAATGGCGAAGTTATTGGTCGTCTCGGCGGTGATGAGTTTTTAGTTGTTTCACTAAACAACGAGAATGCGGATATTTCGTCGCTGCGAGAACGCATTCAGCAGCAAATACGTGGAGAATATCACTTAGGTGATGTTGATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCG  >  CP009273/1872634‑1872931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑