Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,881,557 C→A 22.6% R231L (CGC→CTC)  rnd ← ribonuclease D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,881,5570CA22.6% 880.9 / 178.9 351R231L (CGC→CTC) rndribonuclease D
Reads supporting (aligned to +/- strand):  ref base C (139/132);  new base A (45/35);  total (184/167)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 373 total aligned reads displayed.

TTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATCACGCCAGGCATCTTCCGGCGCAACGACTTCCTGACGACGCATTTGCATCAGGCGGCATTCA  >  CP009273/1881408‑1881706
                                                                                                                                                     |                                                                                                                                                     
tttCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATcgc                                                                                                                                                       <  1:4019515/150‑1 (MQ=255)
tttCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGATCTTCACGCACGACAAAGTTCACCGCCAGATcgc                                                                                                                                                       <  2:5065198/150‑1 (MQ=255)
 ttCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATcgcg                                                                                                                                                      <  2:276188/150‑1 (MQ=255)
  tCCACCAGCGCTAGCAGAGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCgcgc                                                                                                                                                     <  2:3183755/150‑1 (MQ=255)
              agcagcGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTg                                                                                                                                         >  1:5217104/1‑150 (MQ=255)
                 agcGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTgcgc                                                                                                                                      <  1:4694949/150‑1 (MQ=255)
                    gTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGt                                                                                                                                   >  2:4727509/1‑150 (MQ=255)
                    gTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGt                                                                                                                                   >  2:3260323/1‑150 (MQ=255)
                     ttttACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTc                                                                                                                                  <  2:4083766/150‑1 (MQ=255)
                      tttACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCg                                                                                                                                 >  1:422386/1‑150 (MQ=255)
                      tttACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCg                                                                                                                                 >  1:812247/1‑150 (MQ=255)
                        tACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGAGCTCTCGCGCCTTGCGCAGTCGcc                                                                                                                               <  1:3202074/150‑1 (MQ=255)
                          ccGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAg                                                                                                                             <  1:1189777/150‑1 (MQ=255)
                            gTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGAGCTCTCGCGCCTTGCGCAGTCGCCAGTc                                                                                                                           <  2:970807/150‑1 (MQ=255)
                               aaaGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGc                                                                                                                        >  2:1052477/1‑150 (MQ=255)
                                aaGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCt                                                                                                                       <  2:3870735/150‑1 (MQ=255)
                                    ggATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACa                                                                                                                   >  1:3800785/1‑150 (MQ=255)
                                     gATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAg                                                                                                                  <  1:572992/150‑1 (MQ=255)
                                     gATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAg                                                                                                                  >  2:1390791/1‑150 (MQ=255)
                                       tttCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGtt                                                                                                                <  2:2482691/150‑1 (MQ=255)
                                          cGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGAGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCa                                                                                                             >  2:4512434/1‑150 (MQ=255)
                                           gCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAg                                                                                                            >  2:2000459/1‑150 (MQ=255)
                                           gCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAg                                                                                                            <  1:154976/150‑1 (MQ=255)
                                             tACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGAGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGAc                                                                                                          >  1:3146251/1‑150 (MQ=255)
                                              aCCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACGTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACa                                                                                                         <  1:5040505/150‑1 (MQ=255)
                                                cGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGAGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAgg                                                                                                       <  1:1862096/150‑1 (MQ=255)
                                                 gggtAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGGTCGAGCTCTCGCGCCTGGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGc                                                                                                      >  1:5193518/4‑150 (MQ=255)
                                                 ggATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGc                                                                                                      <  1:980098/150‑1 (MQ=255)
                                                 ggATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGc                                                                                                      >  2:287373/1‑150 (MQ=255)
                                                 ggATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGc                                                                                                      >  1:345133/1‑150 (MQ=255)
                                                 ggATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGc                                                                                                      >  1:3570915/1‑150 (MQ=255)
                                                  gATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGcc                                                                                                     <  1:735197/150‑1 (MQ=255)
                                                     aaaCCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGt                                                                                                  >  2:164587/1‑150 (MQ=255)
                                                      aaCCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACTCACGACTAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGtt                                                                                                 <  2:1174038/150‑1 (MQ=255)
                                                      aaCCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGAGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGtt                                                                                                 <  1:2743280/150‑1 (MQ=255)
                                                       aCCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTg                                                                                                >  1:1119506/1‑150 (MQ=255)
                                                       aCCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTg                                                                                                <  2:3011153/150‑1 (MQ=255)
                                                        cccAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTgg                                                                                               >  2:2843418/1‑150 (MQ=255)
                                                           aGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCgt                                                                                            >  2:4971044/1‑150 (MQ=255)
                                                           aGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCgt                                                                                            <  1:1715581/150‑1 (MQ=255)
                                                              cTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGtgtg                                                                                         <  2:1561327/150‑1 (MQ=255)
                                                              cTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGtgtg                                                                                         <  2:4655785/150‑1 (MQ=255)
                                                              cTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGtgtg                                                                                         >  1:3669901/1‑150 (MQ=255)
                                                               tGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGAGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCCGTTGGCGTGTGc                                                                                        >  2:4444909/1‑150 (MQ=255)
                                                                        cGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGcc                                                                               <  1:3349525/150‑1 (MQ=255)
                                                                          ccTAACCGTCCCGGCAGAGCACGCGCGACCGACCACACCTGCTCGTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACCGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAg                                                                             <  2:2322121/150‑1 (MQ=255)
                                                                          ccTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGAGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAg                                                                             <  1:3888098/150‑1 (MQ=255)
                                                                          caaaaaCTTCCCGGCATATAACGCGCTACCGACCACAAATGCACTTCACGCACTACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTAGGCGTGTGCGTAATTGCCAg                                                                             >  2:2095742/4‑150 (MQ=255)
                                                                            tAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGc                                                                           >  1:3608989/1‑150 (MQ=255)
                                                                             aaaCTTCCCGGCCTATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGAACGAGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTAGCAGACAGGCCAGTTGGCGTGTGCGTCATTGCCAGGCa                                                                          >  2:4267435/1‑150 (MQ=255)
                                                                              aaCTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACTACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCAt                                                                         >  1:1633709/1‑150 (MQ=255)
                                                                              aaCTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCAt                                                                         >  1:4153158/1‑150 (MQ=255)
                                                                              aaCTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGAGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCAt                                                                         <  1:2788618/150‑1 (MQ=255)
                                                                                cTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTg                                                                       >  2:3817630/1‑150 (MQ=255)
                                                                                   cccGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGACGGCTCACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGACAGGCAgtggtg                                                                    >  1:2412133/1‑150 (MQ=255)
                                                                                   cccGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGAGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTg                                                                    >  2:2706610/1‑150 (MQ=255)
                                                                                    ccGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGa                                                                   >  2:3711709/1‑150 (MQ=255)
                                                                                     cGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGag                                                                  >  2:584228/1‑149 (MQ=255)
                                                                                      ggCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGata                                                                 <  1:1025627/150‑1 (MQ=255)
                                                                                       gCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGatat                                                                <  1:3724367/150‑1 (MQ=255)
                                                                                         atatAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGatataa                                                              <  1:4542967/150‑3 (MQ=255)
                                                                                         atatAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGatataa                                                              >  2:1747961/1‑148 (MQ=255)
                                                                                            aaaCGCGCTACCGACCACAAAAGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGAGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGc                                                           >  2:3324482/2‑150 (MQ=255)
                                                                                             aaCGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGcc                                                          >  1:4123214/1‑150 (MQ=255)
                                                                                              aCGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCa                                                         <  1:3008300/150‑1 (MQ=255)
                                                                                               cgcgCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAg                                                        <  2:4896305/150‑1 (MQ=255)
                                                                                               cgcgCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGAGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAg                                                        >  2:516350/1‑150 (MQ=255)
                                                                                                  gatACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCa                                                     <  1:1478636/148‑1 (MQ=255)
                                                                                                     aCCGACCACACATGCTCTTCACGCACGACTAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCTGTCGGCTAACAGTGGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCt                                                  <  2:2614892/150‑1 (MQ=255)
                                                                                                        gACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTcc                                               <  2:3464533/150‑1 (MQ=255)
                                                                                                         aCCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCg                                              <  2:3767484/150‑1 (MQ=255)
                                                                                                          ccACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCgg                                             <  1:924778/150‑1 (MQ=255)
                                                                                                           cacaAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGgc                                            <  1:1594008/150‑1 (MQ=255)
                                                                                                              aaaTGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCa                                         <  1:4466657/150‑1 (MQ=255)
                                                                                                                aTGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAAc                                       >  1:2959951/1‑150 (MQ=255)
                                                                                                                     ctTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACtt                                  <  1:3244958/150‑1 (MQ=255)
                                                                                                                        cacgcacgACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCt                               <  2:4171291/150‑1 (MQ=255)
                                                                                                                         acgcacgACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCTg                              >  2:3969990/1‑150 (MQ=255)
                                                                                                                         acgcacgACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCTg                              <  2:1250868/150‑1 (MQ=255)
                                                                                                                         acgcacgACAAAGTTCACCGCCAGATCGAGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCTg                              <  1:2923547/150‑1 (MQ=255)
                                                                                                                           gcacgACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCTgac                            <  1:1315742/150‑1 (MQ=255)
                                                                                                                            cacgACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCTgacg                           >  2:4599243/1‑150 (MQ=255)
                                                                                                                               gACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCTGacgacg                        >  1:1288420/1‑150 (MQ=255)
                                                                                                                               gACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCTGacgacg                        >  2:2293034/1‑150 (MQ=255)
                                                                                                                               gACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACCTCCTGacgacg                        <  1:4971044/150‑1 (MQ=255)
                                                                                                                                aCAAAGTTCACCGCCAGATCGCGATCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCTGACGACGc                       >  2:525024/1‑150 (MQ=255)
                                                                                                                                 cAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCTGACGACGCa                      >  2:72076/1‑150 (MQ=255)
                                                                                                                                  aaaGTTCACCGCCAGATCGCGCTCTCGCGCCTTGTGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCTGACGACGCAt                     <  2:2212271/150‑1 (MQ=255)
                                                                                                                                  aaaGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCTGACGACGCAt                     >  1:645635/1‑150 (MQ=255)
                                                                                                                                   aaGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCTGACGACGCAtt                    <  1:2824170/150‑1 (MQ=255)
                                                                                                                                    aGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCTGACGACGCAttt                   >  2:5015018/1‑150 (MQ=255)
                                                                                                                                    aGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCTGACGACGCAttt                   <  2:3233011/150‑1 (MQ=255)
                                                                                                                                    aGTTCACCGCCAGATCGAGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCTGACGACGCAttt                   <  1:2622643/150‑1 (MQ=255)
                                                                                                                                       tCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCTGACGACGCATTTGCa                >  2:357663/1‑150 (MQ=255)
                                                                                                                                       tCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCTGACGACGCATTTGCa                <  2:5303576/150‑1 (MQ=255)
                                                                                                                                        cACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCTGACGACGCATTTGCAt               >  1:1783773/1‑150 (MQ=255)
                                                                                                                                             ccAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCGGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCTGACGACGCATTTGCATCAggc          >  1:2115888/1‑150 (MQ=255)
                                                                                                                                              cAGATCGAGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCTGACGACGCATTTGCATCAggcg         >  1:1830709/1‑150 (MQ=255)
                                                                                                                                               aGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCTGACGACGCATTTGCATCAggcgg        >  1:2232439/1‑150 (MQ=255)
                                                                                                                                                  tCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATAACGCCAGGCATCTTCCGGCGCAACGACTTCCTGACGACGCATTTGCATCAGGCGGCAt     >  1:229593/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTAAACTTCCCGGCATATAACGCGCTACCGACCACAAATGCTCTTCACGCACGACAAAGTTCACCGCCAGATCGCGCTCTCGCGCCTTGCGCAGTCGCCAGTCGGCTAACAGTTGCAGACAGGCCAGTTGGCGTGTGCGTAATTGCCAGGCATTGGTGATATCACGCCAGGCATCTTCCGGCGCAACGACTTCCTGACGACGCATTTGCATCAGGCGGCATTCA  >  CP009273/1881408‑1881706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑