Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,909,342 T→A 100% E150D (GAA→GAT)  proQ ← RNA chaperone, putative ProP translation regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,909,3420TA100.0% 927.9 / ‑6.7 281E150D (GAA→GAT) proQRNA chaperone, putative ProP translation regulator
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base A (153/126);  minor base C (1/0);  total (155/126)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 290 total aligned reads displayed.

GTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTG  >  CP009273/1909195‑1909488
                                                                                                                                                   |                                                                                                                                                  
gTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCcatc                                                                                                                                                  >  2:1619586/1‑150 (MQ=255)
gTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCcatc                                                                                                                                                  >  1:39734/1‑150 (MQ=255)
 ttttGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCcatct                                                                                                                                                 <  2:2541872/150‑1 (MQ=255)
 ttttGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCcatct                                                                                                                                                 >  2:4143250/1‑150 (MQ=255)
        ccGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTgcggc                                                                                                                                          <  2:3402327/150‑1 (MQ=255)
         cGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTgcggcg                                                                                                                                         <  2:4034170/150‑1 (MQ=255)
          gCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTTCggcgt                                                                                                                                        >  1:1215339/1‑150 (MQ=255)
           cTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGTCTTTTGCTCACGAGGTTTTCGTTCAGCGCCATCTTTGAggcgtg                                                                                                                                       <  2:33247/150‑1 (MQ=255)
              tCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCggcgtggcg                                                                                                                                    >  2:562868/1‑150 (MQ=255)
                aCCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTa                                                                                                                                  <  1:1584854/150‑1 (MQ=255)
                 ccTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAg                                                                                                                                 <  2:4350280/150‑1 (MQ=255)
                  cTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGt                                                                                                                                >  1:1390460/1‑150 (MQ=255)
                  cTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGt                                                                                                                                >  2:4013331/1‑150 (MQ=255)
                     cAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCgg                                                                                                                             >  2:4911705/1‑150 (MQ=255)
                      aGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGa                                                                                                                            >  1:4765342/1‑150 (MQ=255)
                       gggCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGAc                                                                                                                           <  2:5168774/150‑1 (MQ=255)
                         gCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGAACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGt                                                                                                                         >  1:2080624/1‑150 (MQ=255)
                              tCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCtt                                                                                                                    >  2:2860102/1‑150 (MQ=255)
                              tCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCtt                                                                                                                    <  2:2804729/150‑1 (MQ=255)
                               ccGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTg                                                                                                                   <  1:720478/150‑1 (MQ=255)
                                 gACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCg                                                                                                                 >  1:3438277/1‑150 (MQ=255)
                                 gACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCg                                                                                                                 >  2:2041594/1‑150 (MQ=255)
                                    aGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTc                                                                                                              <  1:1466579/150‑1 (MQ=255)
                                     gTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcg                                                                                                             >  1:4873476/1‑150 (MQ=255)
                                     gTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcg                                                                                                             >  1:1067748/1‑150 (MQ=255)
                                      tCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgc                                                                                                            <  1:2697112/150‑1 (MQ=255)
                                      tCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgc                                                                                                            <  1:539582/150‑1 (MQ=255)
                                        agagCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcgg                                                                                                          >  1:2483903/1‑150 (MQ=255)
                                         gagCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggc                                                                                                         <  1:4227382/150‑1 (MQ=255)
                                         gagCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggc                                                                                                         <  2:1011991/150‑1 (MQ=255)
                                          agCTGACATGACAGAAACCGGGGTGTGCTGTTCGTCGCGAGGTGCTTCTACAGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGGTcgcggcg                                                                                                        <  1:4058648/150‑1 (MQ=255)
                                          agCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggcg                                                                                                        >  1:5262277/1‑150 (MQ=255)
                                          agCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTGACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGGTcgcggcg                                                                                                        <  2:155067/150‑1 (MQ=255)
                                            cTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTcgcggcgcg                                                                                                      >  1:3162479/1‑150 (MQ=255)
                                              gAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTACTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGt                                                                                                    >  2:5318280/1‑150 (MQ=255)
                                               aaaTGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCGACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTg                                                                                                   <  1:370120/150‑1 (MQ=255)
                                                aaTGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGc                                                                                                  >  2:2103115/1‑150 (MQ=255)
                                                aaTGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGc                                                                                                  <  1:2217633/150‑1 (MQ=255)
                                                   gTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGtc                                                                                               >  2:3014149/1‑150 (MQ=255)
                                                    tCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGtct                                                                                              <  2:3697114/150‑1 (MQ=255)
                                                     cAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGtctt                                                                                             >  1:5040751/1‑150 (MQ=255)
                                                        aaaCCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTcttctt                                                                                          <  2:5030527/150‑1 (MQ=255)
                                                         aaCCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCttcttt                                                                                         <  1:4260254/150‑1 (MQ=255)
                                                         aaCCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCttcttt                                                                                         <  2:616339/150‑1 (MQ=255)
                                                           ccGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCttctttct                                                                                       <  1:2292183/150‑1 (MQ=255)
                                                           ccGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCttctttct                                                                                       <  1:4027060/150‑1 (MQ=255)
                                                           ccGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCttctttct                                                                                       <  2:735169/150‑1 (MQ=255)
                                                                 tgtgCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACcag                                                                                 >  1:500026/1‑150 (MQ=255)
                                                                   tgCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACcagca                                                                               >  1:3023761/1‑150 (MQ=255)
                                                                       gTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTg                                                                           <  2:383332/150‑1 (MQ=255)
                                                                          cttcGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCgg                                                                        <  1:806875/150‑1 (MQ=255)
                                                                             cgcgAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAg                                                                     >  1:5212830/1‑150 (MQ=255)
                                                                               cgAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCt                                                                   <  1:2391125/150‑1 (MQ=255)
                                                                                gAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCtt                                                                  >  2:3288987/1‑150 (MQ=255)
                                                                                 aGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTc                                                                 >  2:848668/1‑150 (MQ=255)
                                                                                      cTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtt                                                            >  1:593149/1‑150 (MQ=255)
                                                                                      cTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtt                                                            >  1:1555328/1‑150 (MQ=255)
                                                                                      cTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtt                                                            >  2:2293431/1‑150 (MQ=255)
                                                                                       ttttACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGttt                                                           <  2:112400/150‑1 (MQ=255)
                                                                                       ttttACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGttt                                                           <  2:2223204/150‑1 (MQ=255)
                                                                                         ttACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGttttt                                                         >  1:90557/1‑150 (MQ=255)
                                                                                         ttACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGttttt                                                         <  2:3438277/150‑1 (MQ=255)
                                                                                           aCTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTc                                                       >  1:4446189/1‑150 (MQ=255)
                                                                                            cTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCg                                                      <  1:1475251/150‑1 (MQ=255)
                                                                                             tGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGc                                                     >  2:3563717/1‑150 (MQ=255)
                                                                                              gTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCt                                                    >  2:966460/1‑150 (MQ=255)
                                                                                               tttttGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCtt                                                   <  1:3995637/150‑1 (MQ=255)
                                                                                                  ttGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCt                                                <  1:1690908/150‑1 (MQ=255)
                                                                                                      cgcTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTc                                            >  2:1887059/1‑150 (MQ=255)
                                                                                                      cgcTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTc                                            >  2:4437389/1‑150 (MQ=255)
                                                                                                       gcTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCa                                           <  1:957256/150‑1 (MQ=255)
                                                                                                       gcTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCa                                           >  1:3553019/1‑150 (MQ=255)
                                                                                                       gcTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTGTTTTCGCTTGCTGTTCa                                           >  1:1879683/1‑150 (MQ=255)
                                                                                                         tttCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGc                                         >  2:4827854/1‑150 (MQ=255)
                                                                                                           tctcTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCAc                                       >  1:2130047/1‑150 (MQ=255)
                                                                                                              ctACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCt                                    >  2:2972463/1‑150 (MQ=255)
                                                                                                                aCCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCtgt                                  <  1:4731143/150‑1 (MQ=255)
                                                                                                                aCCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCtgt                                  <  2:1703126/150‑1 (MQ=255)
                                                                                                                 ccGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCtgtg                                 >  1:259751/1‑150 (MQ=255)
                                                                                                                 ccGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCtgtg                                 <  2:393510/150‑1 (MQ=255)
                                                                                                                  cGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGc                                >  1:2933871/1‑150 (MQ=255)
                                                                                                                   ggCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGcc                               <  1:1054232/150‑1 (MQ=255)
                                                                                                                   ggCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGcc                               >  1:3870798/1‑150 (MQ=255)
                                                                                                                   ggCTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGACGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGcc                               >  2:2660176/1‑150 (MQ=255)
                                                                                                                     cTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTg                             >  1:4565136/1‑150 (MQ=255)
                                                                                                                     cTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTg                             >  1:2360301/1‑150 (MQ=255)
                                                                                                                     cTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTg                             >  2:2493949/1‑150 (MQ=255)
                                                                                                                     cTTTTGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTg                             >  2:1321133/1‑150 (MQ=255)
                                                                                                                      ttttGCGCACGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGa                            >  1:5109655/1‑150 (MQ=255)
                                                                                                                          gcgcACGAGGTTTACGTTCAGCGCCATCTGTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAacac                        <  2:879425/150‑1 (MQ=255)
                                                                                                                              aCGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACgcgc                    >  1:4652327/1‑150 (MQ=255)
                                                                                                                               cGAGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCt                   <  1:5187431/150‑1 (MQ=255)
                                                                                                                               cGAGGTTGACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCt                   <  2:5183670/150‑1 (MQ=255)
                                                                                                                                 aGGTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCttt                 >  2:456216/1‑150 (MQ=255)
                                                                                                                                  ggTTTACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTc                <  2:39734/150‑1 (MQ=255)
                                                                                                                                    tttACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGc              >  1:4709643/1‑150 (MQ=255)
                                                                                                                                     ttACGTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGct             <  2:941578/150‑1 (MQ=255)
                                                                                                                                         gTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGcttctt         >  1:4346846/1‑150 (MQ=255)
                                                                                                                                         gTTCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGcttctt         >  2:1643805/1‑150 (MQ=255)
                                                                                                                                          ttCAGCGCCATCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCttcttc        <  2:4733416/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTTTTGACCCGCTTTCACCTTCAGGGCTTGTCCGACAGTCAGAGCTGAAATGTCAGAAACCGGGGTGTGCTGTTCTTCGCGAGGTGCTTTTACTGTTTTTGGCGCTTTCTCTACCGGCTTTTGCGCACGAGGTTTACGTTCAGCGCCTTCTTTGCGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTG  >  CP009273/1909195‑1909488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑