Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,928,359 A→C 5.8% F168C (TTT→TGT)  edd ← 6‑phosphogluconate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,928,3590AC 5.8% 813.3 / 6.9 333F168C (TTT→TGT) edd6‑phosphogluconate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (178/127);  new base C (2/26);  total (180/153)
Fisher's exact test for biased strand distribution p-value = 7.62e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 341 total aligned reads displayed.

GCGCATGGTAAGACGCGGCTTCTGACTCCAGTAAGGCCATGCGGTCCACTTTACCTTCGGCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGC  >  CP009273/1928210‑1928508
                                                                                                                                                     |                                                                                                                                                     
gcgcATGGTAAGACGCGGCTTCTGACTCCAGTAAGGCCATGCGGTCCACTTTACCTTCGGCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCaa                                                                                                                                                       <  1:112392/150‑1 (MQ=255)
  gcATGGTAAGACGCGGCTTCTGACTCCAGTAAGGCCATGCGGTCCACTTTACCTTCGGCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAAc                                                                                                                                                     <  1:3737024/150‑1 (MQ=255)
     tGGTAAGACGCGGCTTCTGACTCCAGTAAGGCCATGCGGTCCACTTTACCTTCGGCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGAc                                                                                                                                                  >  2:263073/1‑150 (MQ=255)
     tGGTAAGACGCGGCTTCTGACTCCAGTAAGGCCATGCGGTCCACTTTACCTTCGGCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGAc                                                                                                                                                  >  1:1000753/1‑150 (MQ=255)
               cgGCTTCTGACTCCAGTAAGGCCATGCGGTCCACTTTACCTTCGGCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCa                                                                                                                                        >  2:2610715/1‑150 (MQ=255)
               cgGCTTCTGACTCCAGTAAGGCCATGCGGTCCACTTTACCTTCGGCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCa                                                                                                                                        >  2:4403422/1‑150 (MQ=255)
                  cTTCTGACTCCAGTAAGGCCATGCGGTCCACTTTACCTTCGGCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCg                                                                                                                                     >  1:5309474/1‑150 (MQ=255)
                      tGACTCCAGTAAGGCCATGCGGTCCACTTTACCTTCGGCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAg                                                                                                                                 >  1:4399721/1‑150 (MQ=255)
                          tCCAGTAAGGCCATGCGGTCCACTTTACCTTCGGCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGAccc                                                                                                                             >  2:2567912/1‑150 (MQ=255)
                             aGTAAGGCCCTGCGGTCCACTTTACCTTCGGCATAAAGCTGGCTAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACCCCGCTGGCAAATGACCACACGACAGGGCTGCCATCGTCAGACCCggg                                                                                                                          <  2:2362963/150‑1 (MQ=255)
                             aGTAAGGCCATGCGGTCCACTTTACCTTCGGCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCggg                                                                                                                          >  1:332705/1‑150 (MQ=255)
                                aaGGCCATGCGGTCCACTTTACCTTCGGCATAAAGCTGGCGAATACGCACTTTTTCTATATTTGGCAAACCGCTAGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGACAGACCCCGGACa                                                                                                                       >  2:5251679/1‑150 (MQ=255)
                                 cgcccttGCGGTCCACTTTACCTACGAAATAAAGCTGGCTAATACGCACTTTTTCTTTATTGGCCAACCCGCTTGCAATCGGTCCAGACGGCACAAACACCGCAGCCAAATCACCACACGACAGGGCTGCCATCGTCAGACCCGGGACaa                                                                                                                      <  1:473160/144‑1 (MQ=255)
                                      aTGCGGTCCACTTTACCTTCGGCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTg                                                                                                                 >  1:2747680/1‑150 (MQ=255)
                                          ggTCCACTTTACCTTCGGCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGc                                                                                                             >  2:3321368/1‑150 (MQ=255)
                                            tCCACTTTACCTTCGGCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGcac                                                                                                           >  2:4126689/1‑150 (MQ=255)
                                             ccACTTTACCTTCGGCATAACGCTGGCGAATACGCACTTTTTATTTATTTGGCAAACCGCTTGCCATCGGTCCAGCCGGCACAAACACCGCTGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGcaca                                                                                                          <  1:1207650/150‑1 (MQ=255)
                                                 tttACCTTCGGCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACAcc                                                                                                      >  1:4980077/1‑150 (MQ=255)
                                                    aCCTTCGGCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAACCCGCTTGCCATCGGTCCAGACGGCACACACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAg                                                                                                   <  2:1300729/150‑1 (MQ=255)
                                                    aCCTTCGGCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAg                                                                                                   <  1:787167/150‑1 (MQ=255)
                                                    aCCTTCGGCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAg                                                                                                   <  1:3458079/150‑1 (MQ=255)
                                                       ttCGGCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGaa                                                                                                <  2:3242298/150‑1 (MQ=255)
                                                        tCGGCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAAc                                                                                               <  2:2997954/150‑1 (MQ=255)
                                                         gggCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAACCCGCTTGCCATCGGACCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACa                                                                                              <  2:4563528/149‑1 (MQ=255)
                                                         cGGCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACCCCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACa                                                                                              <  2:109482/150‑1 (MQ=255)
                                                          ggaaTAAAGCTGGCGAATACGAAATTTTTCTTTATTGGCCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCACACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACag                                                                                             <  1:1553093/147‑1 (MQ=255)
                                                          ggCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACag                                                                                             >  1:4033741/1‑150 (MQ=255)
                                                            cATAAAGCTGGAGAATACGAAATTTTTATTTATTTGGAAAACCGCTTGCCATCGGCCCTGCCGGCACTCACACGGCGGGACTTTGCCCACACGACAGGGCTGCCATCGTCAGACCCGGGACTCTCTTGTCGCACACTCCGTGGTACagtg                                                                                           <  2:614690/150‑3 (MQ=255)
                                                            aaTAACGCTGGCTAATACGATGTTTTTTTTTATTGGGCAACCCGCTGGCCTTGGGACCCGCCGCCACAAACACCGCTGTAAATTCACAACACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACagag                                                                                           <  1:4485795/149‑1 (MQ=255)
                                                               aaaGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCAc                                                                                        >  1:3088697/1‑150 (MQ=255)
                                                                 aGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCa                                                                                      >  2:4649690/1‑150 (MQ=255)
                                                                 aGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCa                                                                                      >  2:1824254/1‑150 (MQ=255)
                                                                   cTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATc                                                                                    >  1:685061/1‑150 (MQ=255)
                                                                       cGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAAc                                                                                >  2:2461991/1‑150 (MQ=255)
                                                                         aaTACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCACATGACCAAACGACAGGGCTGCCATCGTCAGCCCCGGGACAATCTTGTCGCACACACCGAGGCCCAGAGCACCCTCCAACAt                                                                              >  2:188058/1‑150 (MQ=255)
                                                                         aaTACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACAt                                                                              >  2:4470086/1‑150 (MQ=255)
                                                                          aTACGAACTTTTTCTTTATTTGGCAAACCGCTTGCAATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATg                                                                             <  1:4414124/150‑1 (MQ=255)
                                                                           tACGAATTTTTTCTTTATTTGGAAAACCGCTTGCAATGGGTACAGACGGCACAAACACGGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGt                                                                            <  2:1904627/150‑1 (MQ=255)
                                                                               cACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGTCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATg                                                                        <  2:390480/150‑1 (MQ=255)
                                                                                aCTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATgg                                                                       >  2:2861542/1‑150 (MQ=255)
                                                                                 atttttATTTTTTTGTCAAAGCGTTTGCCATCTTTCCAGCCGGCACAAACACGGCAGGCAATTGTCCACACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATggg                                                                      <  1:2595646/149‑1 (MQ=255)
                                                                                  tttttCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGa                                                                     >  2:749392/1‑150 (MQ=255)
                                                                                  tttttCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCACAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGa                                                                     <  2:2108353/150‑1 (MQ=255)
                                                                                   ttttCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGAc                                                                    <  2:2913620/150‑1 (MQ=255)
                                                                                   ttttCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGAc                                                                    <  2:3188394/150‑1 (MQ=255)
                                                                                   ttttCTTTATTTGCAAAACCGCTTGCCATCGGACCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGAc                                                                    <  1:4550701/150‑1 (MQ=255)
                                                                                   ttttCTTTATTGGGCAACCCGCTTGCCATCGGTCCAGACGGCACAAACACCGCCGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGAc                                                                    <  2:1654754/150‑1 (MQ=255)
                                                                                    tttCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACa                                                                   <  2:4301793/150‑1 (MQ=255)
                                                                                       cTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGcc                                                                >  2:2349918/1‑150 (MQ=255)
                                                                                       cTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGcc                                                                >  2:1569730/1‑150 (MQ=255)
                                                                                         ttATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGcccc                                                              >  1:2950196/1‑150 (MQ=255)
                                                                                            tttGGCAAACCGCTTGCCATCGGACCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCAcc                                                           <  1:882913/150‑1 (MQ=255)
                                                                                             ttGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCAccg                                                          >  2:2132341/1‑150 (MQ=255)
                                                                                             ttGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCAccg                                                          >  1:4083089/1‑150 (MQ=255)
                                                                                               ggCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCAccgcc                                                        <  1:4302728/150‑1 (MQ=255)
                                                                                                gCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCAccgccg                                                       >  2:3697596/1‑150 (MQ=255)
                                                                                                  aaaCCGCTTGCCATCGGTCCAGCCGGCAAAAACCCCGCCGGCAAATGCCCACACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCa                                                     <  1:1204515/150‑1 (MQ=255)
                                                                                                  aaaCCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCa                                                     >  2:1790182/1‑150 (MQ=255)
                                                                                                  aaaCCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCa                                                     >  2:4760815/1‑150 (MQ=255)
                                                                                                  aaaCCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCa                                                     >  2:5117660/1‑150 (MQ=255)
                                                                                                   aaacGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGTGACAGCCCCACCGCCGCAg                                                    >  1:1944774/4‑150 (MQ=255)
                                                                                                   aaCCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAg                                                    >  2:1182343/1‑150 (MQ=255)
                                                                                                        cTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATc                                               >  2:2949835/1‑150 (MQ=255)
                                                                                                         ttGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCg                                              >  1:1813526/1‑150 (MQ=255)
                                                                                                         ttGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCg                                              >  2:2509469/1‑150 (MQ=255)
                                                                                                         ttGCCATCGGTCCAGACGGCAAAAACACCGCANGAAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACTTGTTATGGGACAGCCCCACCGCCGCAGACATcn                                              <  1:1487013/150‑2 (MQ=255)
                                                                                                              aTCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATc                                         >  1:3566826/1‑150 (MQ=255)
                                                                                                              aTCGGCCCAGACGGCACAACCCCCGCCGGCAAATGCCCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATc                                         <  1:3635103/150‑1 (MQ=255)
                                                                                                               tCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCa                                        >  2:3954010/1‑150 (MQ=255)
                                                                                                                  gTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACtt                                     >  2:1359020/1‑150 (MQ=255)
                                                                                                                   tCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTc                                    <  1:2449435/150‑1 (MQ=255)
                                                                                                                     cAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTcgc                                  <  2:1502561/150‑1 (MQ=255)
                                                                                                                      aGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTcgcg                                 >  1:513137/1‑150 (MQ=255)
                                                                                                                      aGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTcgcg                                 >  2:2219110/1‑150 (MQ=255)
                                                                                                                       gACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCgg                                <  1:2115684/150‑1 (MQ=255)
                                                                                                                       gACGGCACAAACACCGCAGGCAAAGGACCACACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCgg                                <  1:4190411/150‑1 (MQ=255)
                                                                                                                        aCGGCACAAACACCGCAGGCAAATTACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACTGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGc                               <  2:2972952/150‑1 (MQ=255)
                                                                                                                         cGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCt                              <  2:4747307/150‑1 (MQ=255)
                                                                                                                          ggCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCtt                             >  2:3593513/1‑150 (MQ=255)
                                                                                                                            cacaAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTag                           <  1:2050241/150‑1 (MQ=255)
                                                                                                                            cacaAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTag                           >  1:38629/1‑150 (MQ=255)
                                                                                                                             acaAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTagc                          >  2:1700456/1‑150 (MQ=255)
                                                                                                                             acaAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTagc                          <  1:1188050/150‑1 (MQ=255)
                                                                                                                              caAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTagca                         >  1:2645958/1‑150 (MQ=255)
                                                                                                                               aaaCACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGACCAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTagcag                        >  2:681397/1‑150 (MQ=255)
                                                                                                                                  cacCTCTGGCAATTTACAAAACGACAGGGCTGCCATCGTCAGACCCGTGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTATCAGCGa                     <  1:2996337/150‑1 (MQ=255)
                                                                                                                                     cGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACaa                  >  1:4867173/1‑150 (MQ=255)
                                                                                                                                      gCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAat                 <  1:5168058/150‑1 (MQ=255)
                                                                                                                                      gCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAat                 <  1:595211/150‑1 (MQ=255)
                                                                                                                                            aaaTGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAattccatt           <  1:1762061/150‑1 (MQ=255)
                                                                                                                                            aaaTGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAattccatt           >  1:3200880/1‑150 (MQ=255)
                                                                                                                                            aaaTGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAattccatt           >  2:2731596/1‑150 (MQ=255)
                                                                                                                                              aTGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAattccattcc         >  1:3655333/1‑150 (MQ=255)
                                                                                                                                               ttaCCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAAttccattcca        <  1:251872/148‑1 (MQ=255)
                                                                                                                                                 aCCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAattccatt           >  2:5059817/1‑145 (MQ=255)
                                                                                                                                                  ccAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATcc     >  2:3777749/1‑150 (MQ=255)
                                                                                                                                                   cAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCt    <  1:4692758/150‑1 (MQ=255)
                                                                                                                                                   cAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCt    <  2:958029/150‑1 (MQ=255)
                                                                                                                                                    aaaCGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTg   >  1:295852/1‑150 (MQ=255)
                                                                                                                                                     aaCGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGc  >  1:1304967/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCGCATGGTAAGACGCGGCTTCTGACTCCAGTAAGGCCATGCGGTCCACTTTACCTTCGGCATAAAGCTGGCGAATACGCACTTTTTCTTTATTTGGCAAACCGCTTGCCATCGGTCCAGACGGCACAAACACCGCAGGCAAATGACCAAACGACAGGGCTGCCATCGTCAGACCCGGGACAATCTTGTCGCACACACCGAGGAACAGAGCACCATCAAACATGTTATGGGACAGCCCCACCGCCGCAGACATCGCTATCACTTCGCGGCTTAGCAGCGACAATTCCATTCCATCCTGC  >  CP009273/1928210‑1928508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑