BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,933,029	A→T	6.7%	H375L (CAC→CTC)	pykA →	pyruvate kinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,933,029	0	A	T	6.7%	954.2 / 41.1	309	H375L (CAC→CTC)	pykA	pyruvate kinase II
Reads supporting (aligned to +/- strand): ref base A (160/128); new base T (12/9); total (172/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 323 total aligned reads displayed.

TATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGC  >  CP009273/1932881‑1933178

    cGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGa                                                                                                                                                  >  2:4539788/1‑150 (MQ=255)

       cAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAg                                                                                                                                               <  2:3756173/150‑1 (MQ=255)

         gAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGc                                                                                                                                             <  2:2865229/150‑1 (MQ=255)

          aaaCCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCg                                                                                                                                            <  2:392705/150‑1 (MQ=255)

          aaaCCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCg                                                                                                                                            >  1:1530019/1‑150 (MQ=255)

              cGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTAc                                                                                                                                        <  2:2371647/150‑1 (MQ=255)

                 tGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGc                                                                                                                                     >  1:4562064/1‑150 (MQ=255)

                  gCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCg                                                                                                                                    >  1:3492758/1‑150 (MQ=255)

                   cAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGa                                                                                                                                   >  2:4494451/1‑150 (MQ=255)

                    aGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCTCCTGAAAGGCGTTACGGCGat                                                                                                                                  <  2:381025/150‑1 (MQ=255)

                      ccATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGatca                                                                                                                                >  1:4976524/1‑150 (MQ=255)

                             gcgcgTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCAt                                                                                                                         <  2:2286125/150‑1 (MQ=255)

                             gcgcgTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCAt                                                                                                                         <  1:1489262/150‑1 (MQ=255)

                                cgTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGAc                                                                                                                      <  2:681031/150‑1 (MQ=255)

                                 gTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCTCCTGAAAGGCGTTACGGCGATCATCACCATGAcc                                                                                                                     <  2:1781110/150‑1 (MQ=255)

                                 gTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGAcc                                                                                                                     >  1:544479/1‑150 (MQ=255)

                                  tttGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCg                                                                                                                    <  2:3123971/150‑1 (MQ=255)

                                     gCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCTCCTGAAAGGCGTTACGGCGATCATCACCATGACCGAAt                                                                                                                 <  2:105155/150‑1 (MQ=255)

                                     gCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAAt                                                                                                                 <  1:3709746/150‑1 (MQ=255)

                                      ccTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATc                                                                                                                <  1:4444340/150‑1 (MQ=255)

                                         gggTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACACTGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCggg                                                                                                             <  1:2782907/150‑1 (MQ=255)

                                           gTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTc                                                                                                           >  1:4999270/1‑150 (MQ=255)

                                             gCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGt                                                                                                         <  2:4728558/150‑1 (MQ=255)

                                              cGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTa                                                                                                        >  1:2464499/1‑150 (MQ=255)

                                               ggAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTAc                                                                                                       >  1:4860259/1‑150 (MQ=255)

                                                  aaaaaTCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACcgc                                                                                                    >  1:3583199/1‑150 (MQ=255)

                                                     aaTCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCt                                                                                                 <  1:172903/150‑1 (MQ=255)

                                                      aTCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCtg                                                                                                <  2:5236532/150‑1 (MQ=255)

                                                        cccGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCTCCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGGCGTACCGCGCtgat                                                                                              <  2:2781842/150‑1 (MQ=255)

                                                        cccGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCtgat                                                                                              <  1:2577975/150‑1 (MQ=255)

                                                        cccGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCtgat                                                                                              <  1:124378/150‑1 (MQ=255)

                                                         ccGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCtgatg                                                                                             >  1:4404094/1‑150 (MQ=255)

                                                              cATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTc                                                                                        <  1:676960/150‑1 (MQ=255)

                                                                taaaCGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAATGCGTTAAGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTccc                                                                                      <  2:446310/148‑1 (MQ=255)

                                                                tCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTccc                                                                                      <  1:3399503/150‑1 (MQ=255)

                                                                  aaCGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGt                                                                                    >  1:1841987/1‑150 (MQ=255)

                                                                  aaCGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGt                                                                                    >  1:579804/1‑150 (MQ=255)

                                                                    cGTTTCGAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTAt                                                                                  <  1:4728425/150‑1 (MQ=255)

                                                                      tttCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCa                                                                                <  2:914633/150‑1 (MQ=255)

                                                                         cTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGct                                                                             >  2:4187538/1‑150 (MQ=255)

                                                                           aaaCACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGctct                                                                           <  1:2262557/150‑1 (MQ=255)

                                                                           aaaCACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGctct                                                                           <  1:3200526/150‑1 (MQ=255)

                                                                            aaCACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTAGTGCCATGTCAGCAATGTACGCAGCTACCCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTg                                                                          <  2:3391306/150‑1 (MQ=255)

                                                                               acCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTc                                                                       >  2:1591265/1‑150 (MQ=255)

                                                                               acCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTc                                                                       >  2:1342557/1‑150 (MQ=255)

                                                                                     tGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCTCCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGGCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCaa                                                                 >  1:2888700/1‑150 (MQ=255)

                                                                                      ggACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAAt                                                                >  1:1578777/1‑150 (MQ=255)

                                                                                       gACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAAtt                                                               >  1:100920/1‑150 (MQ=255)

                                                                                        aCGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAAttt                                                              >  2:2128231/1‑150 (MQ=255)

                                                                                          gTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTc                                                            >  2:2539275/1‑150 (MQ=255)

                                                                                          gTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTc                                                            >  2:3659879/1‑150 (MQ=255)

                                                                                           ttCAGTTCGACAATGTTGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCg                                                           <  2:3058859/150‑1 (MQ=255)

                                                                                           ttCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCg                                                           <  1:4373141/150‑1 (MQ=255)

                                                                                              aGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCa                                                        >  1:3471417/1‑150 (MQ=255)

                                                                                                ttCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATg                                                      <  1:3911712/150‑1 (MQ=255)

                                                                                                ttCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATg                                                      <  2:3241700/150‑1 (MQ=255)

                                                                                                  cGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTc                                                    <  1:995258/150‑1 (MQ=255)

                                                                                                   gACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTcg                                                   <  2:5313485/150‑1 (MQ=255)

                                                                                                    aCAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTcgc                                                  >  1:3412432/1‑150 (MQ=255)

                                                                                                        tgtgGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCa                                              <  2:4406791/150‑1 (MQ=255)

                                                                                                        tgtgGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCa                                              >  2:2970712/1‑150 (MQ=255)

                                                                                                         gtgGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCAt                                             >  2:2203434/1‑150 (MQ=255)

                                                                                                         gtgGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCAt                                             <  1:196240/150‑1 (MQ=255)

                                                                                                             aagaagCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAAc                                         >  2:2030969/1‑150 (MQ=255)

                                                                                                             aagaagCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAAc                                         >  2:4871879/1‑150 (MQ=255)

                                                                                                               gaagCTATTGCCCTGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGt                                       <  2:3424054/150‑1 (MQ=255)

                                                                                                                aagCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTa                                      <  1:884006/150‑1 (MQ=255)

                                                                                                                 agCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTAc                                     <  2:3492512/150‑1 (MQ=255)

                                                                                                                   cTATTGCCATGTCAGCAATGTACGCAGCTAACCTCCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGc                                   >  2:796045/1‑150 (MQ=255)

                                                                                                                    tATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCt                                  >  1:3347996/1‑150 (MQ=255)

                                                                                                                          cATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCt                            <  2:2077256/150‑1 (MQ=255)

                                                                                                                           aTGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTg                           >  2:4291103/1‑150 (MQ=255)

                                                                                                                            tGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGa                          >  1:2853928/1‑150 (MQ=255)

                                                                                                                             gTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGAc                         <  1:4219748/150‑1 (MQ=255)

                                                                                                                              tCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACt                        <  1:2680805/150‑1 (MQ=255)

                                                                                                                               cAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTg                       <  1:2297054/150‑1 (MQ=255)

                                                                                                                               cAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTg                       <  1:233270/150‑1 (MQ=255)

                                                                                                                                aGCAATGTACGCAGCTAACCTCCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGGCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGc                      >  2:3641770/1‑150 (MQ=255)

                                                                                                                                aGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGc                      <  1:2148713/150‑1 (MQ=255)

                                                                                                                                  cAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGACCGTACGCTGAACCTGACTGctc                    >  1:662361/1‑150 (MQ=255)

                                                                                                                                   aaTGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGctct                   >  2:2647728/1‑150 (MQ=255)

                                                                                                                                    aTGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGctctc                  >  1:240502/1‑150 (MQ=255)

                                                                                                                                    aTGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGctctc                  >  1:480701/1‑150 (MQ=255)

                                                                                                                                    aTGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGctctc                  >  2:561167/1‑150 (MQ=255)

                                                                                                                                          gCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGt            >  2:1524370/1‑150 (MQ=255)

                                                                                                                                          gCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGt            >  1:4041918/1‑150 (MQ=255)

                                                                                                                                           cAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTg           >  1:141978/1‑150 (MQ=255)

                                                                                                                                            aGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTgg          >  2:412918/1‑150 (MQ=255)

                                                                                                                                            aGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTgg          <  2:3529135/150‑1 (MQ=255)

                                                                                                                                              ntAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCg        >  1:542571/2‑150 (MQ=255)

                                                                                                                                                aacaacCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGtt      >  2:4014947/1‑150 (MQ=255)

                                                                                                                                                aaCCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGtt      <  1:1128210/150‑1 (MQ=255)

                                                                                                                                                aaCCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGtt      >  2:3614348/1‑150 (MQ=255)

                                                                                                                                                aaCCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGtt      >  2:2655054/1‑150 (MQ=255)

                                                                                                                                                aaCCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGtt      <  1:5185825/150‑1 (MQ=255)

                                                                                                                                                aaCCACCTGAAAGGAGTTACGGCGATCATCACCAGTACCGTATCGTGTCGTACCGCGATGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCTCGACATGAACGTACGCTGTACCTTTCTGCTCTCTATCGTGGCGtt      <  2:641689/150‑1 (MQ=255)

                                                                                                                                                 acctccTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGGCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTa     >  2:5058469/1‑150 (MQ=255)

                                                                                                                                                 acctccTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGGCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTa     >  2:4374692/1‑150 (MQ=255)

                                                                                                                                                    accTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGc  <  2:4244976/150‑1 (MQ=255)

                                                                                                                                                    accTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGc  <  2:1262568/150‑1 (MQ=255)

TATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCATGTCGCGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGTGGCGTTACGC  >  CP009273/1932881‑1933178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`