Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 185,393 A→T 100% F201I (TTC→ATC)  map ← methionine aminopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273185,3930AT99.7% 932.1 / ‑4.8 284F201I (TTC→ATC) mapmethionine aminopeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (142/141);  total (142/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 284 total aligned reads displayed.

GCGTCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCG  >  CP009273/185246‑185539
                                                                                                                                                   |                                                                                                                                                  
        agttCGCAGCCGTTATCAGTCACCACAATAGTAGGCTCATATTGTGCAGTCAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGAGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCa                                                                                                                                          <  2:160724/148‑1 (MQ=255)
        aTTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTTCCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCa                                                                                                                                          >  1:3655985/1‑150 (MQ=255)
        aTTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCa                                                                                                                                          <  2:4082723/150‑1 (MQ=255)
         tttCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAg                                                                                                                                         >  1:2242513/1‑150 (MQ=255)
          ttCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTTACCATTTGCTCGATGGTTATCGTCATCCCAgg                                                                                                                                        >  1:938665/1‑150 (MQ=255)
          ttCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAgg                                                                                                                                        <  1:799647/150‑1 (MQ=255)
           tCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGt                                                                                                                                       <  2:980432/150‑1 (MQ=255)
            cGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTATTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATAGTCATCCCAGGtt                                                                                                                                      >  1:4995111/1‑150 (MQ=255)
             gCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGttt                                                                                                                                     <  2:2529719/150‑1 (MQ=255)
               aGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCa                                                                                                                                   >  1:4582253/1‑150 (MQ=255)
                  cGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTa                                                                                                                                >  1:4338181/1‑150 (MQ=255)
                   gTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTac                                                                                                                               <  1:3899822/150‑1 (MQ=255)
                   gTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTac                                                                                                                               <  2:2085306/150‑1 (MQ=255)
                      aTCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTacgac                                                                                                                            <  2:350447/150‑1 (MQ=255)
                      aTCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTacgac                                                                                                                            <  2:2039956/150‑1 (MQ=255)
                              ccacAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGttt                                                                                                                    <  2:3283690/150‑1 (MQ=255)
                               cacaATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTc                                                                                                                   >  2:4700830/1‑150 (MQ=255)
                                  aaTAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGTTGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACg                                                                                                                <  1:2544328/150‑1 (MQ=255)
                                  aaTAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACg                                                                                                                <  2:2007656/150‑1 (MQ=255)
                                     aGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGa                                                                                                             >  1:4589596/1‑150 (MQ=255)
                                          gCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCAt                                                                                                        <  2:2752110/150‑1 (MQ=255)
                                          gCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCAt                                                                                                        <  1:2478753/150‑1 (MQ=255)
                                            tCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAg                                                                                                      <  1:33733/150‑1 (MQ=255)
                                             cATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGt                                                                                                     <  2:2850919/150‑1 (MQ=255)
                                                atTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTgca                                                                                                  <  2:3608167/150‑1 (MQ=255)
                                                atTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTgca                                                                                                  >  1:1658234/1‑150 (MQ=255)
                                                   gtgCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTgcagca                                                                                               >  1:4186075/1‑150 (MQ=255)
                                                      cAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCt                                                                                            <  2:167397/150‑1 (MQ=255)
                                                      cAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCt                                                                                            <  2:1342500/150‑1 (MQ=255)
                                                       aGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTg                                                                                           >  1:5258718/1‑150 (MQ=255)
                                                        gACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGc                                                                                          <  1:3693474/150‑1 (MQ=255)
                                                        gACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGc                                                                                          >  2:2524790/1‑150 (MQ=255)
                                                         aCAAGCTGCGATCTTAGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCg                                                                                         >  2:1791442/1‑150 (MQ=255)
                                                          cAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCgg                                                                                        >  2:705841/1‑150 (MQ=255)
                                                           aaGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGt                                                                                       >  1:4666005/1‑150 (MQ=255)
                                                             gCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGttc                                                                                     >  2:5012680/1‑150 (MQ=255)
                                                               tGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGttctt                                                                                   <  1:2831792/150‑1 (MQ=255)
                                                               tGCGATCGTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGAACTCTTTTTTACCCGCGTTGACCATTGGCTCGAGGGTGATCGTCATCCCAGGTTTCAGTACGACGATGGTTACACGGGAGTCAGAGTGCAGCACCTGCGGttcta                                                                                   >  2:540732/1‑149 (MQ=255)
                                                                gCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCCTGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGttcttc                                                                                  <  2:4408567/150‑1 (MQ=255)
                                                                 cGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCa                                                                                 >  1:1027421/1‑150 (MQ=255)
                                                                 cGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCa                                                                                 <  1:1886277/150‑1 (MQ=255)
                                                                    tCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTAAGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCTTgg                                                                              <  1:5172910/150‑1 (MQ=255)
                                                                     cTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGa                                                                             <  2:3040222/150‑1 (MQ=255)
                                                                      tttGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTAGATGGTGATCGTCATCCCATGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGaa                                                                            >  2:1213392/1‑150 (MQ=255)
                                                                          gTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCcg                                                                        <  2:4149123/150‑1 (MQ=255)
                                                                             tttACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGa                                                                     >  1:4047149/1‑150 (MQ=255)
                                                                               tACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGAcc                                                                   >  2:3739369/1‑150 (MQ=255)
                                                                               tACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGAcc                                                                   >  2:918903/1‑150 (MQ=255)
                                                                                aCCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCa                                                                  >  1:4766672/1‑150 (MQ=255)
                                                                                aCCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCa                                                                  >  2:3454165/1‑150 (MQ=255)
                                                                                  cGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAAt                                                                >  1:216425/1‑150 (MQ=255)
                                                                                       aGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCgt                                                           <  1:3959552/150‑1 (MQ=255)
                                                                                       aGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCgt                                                           >  1:1573160/1‑150 (MQ=255)
                                                                                         ccATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCgtgt                                                         >  2:404492/1‑150 (MQ=255)
                                                                                         ccATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCgtgt                                                         <  1:3569879/150‑1 (MQ=255)
                                                                                          cATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATCGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTc                                                        >  2:3528/1‑150 (MQ=255)
                                                                                          cATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTc                                                        <  2:768999/150‑1 (MQ=255)
                                                                                          cATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTc                                                        <  1:4464897/150‑1 (MQ=255)
                                                                                          cATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTc                                                        <  1:1791442/150‑1 (MQ=255)
                                                                                           aTCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTcc                                                       <  1:2533310/150‑1 (MQ=255)
                                                                                           aTCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTcc                                                       >  1:5318718/1‑150 (MQ=255)
                                                                                            tCTTTCATGGTGCGGATCTATTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCg                                                      <  2:2461953/150‑1 (MQ=255)
                                                                                                  aTGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAatat                                                <  1:3106922/150‑1 (MQ=255)
                                                                                                    ggTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTc                                              <  1:1376956/150‑1 (MQ=255)
                                                                                                        cGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacga                                          >  1:4737977/1‑150 (MQ=255)
                                                                                                         ggATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaa                                         >  2:3813457/1‑150 (MQ=255)
                                                                                                          gATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaac                                        >  1:2203816/1‑150 (MQ=255)
                                                                                                          gATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaac                                        <  2:5145674/150‑1 (MQ=255)
                                                                                                            tctcTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaacga                                      >  2:61329/1‑150 (MQ=255)
                                                                                                            tctcTTTTTTACCCGCGTTGACCATAGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCacgaacga                                      >  2:1472813/1‑150 (MQ=255)
                                                                                                             ctctTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAacgac                                     >  1:2719489/1‑150 (MQ=255)
                                                                                                             ctctTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAacgac                                     <  2:4176879/150‑1 (MQ=255)
                                                                                                             ctctTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAacgac                                     <  2:2215772/150‑1 (MQ=255)
                                                                                                              tctTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAacgacg                                    <  1:2040362/150‑1 (MQ=255)
                                                                                                               ctTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCTCCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACgg                                   >  2:5268533/1‑150 (MQ=255)
                                                                                                               ctTTTTTACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACgg                                   >  2:1076823/1‑150 (MQ=255)
                                                                                                               ctTTATTACCCGCGTTGACCATTGGCTCGATGCTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCACTATTCACGAACGACgg                                   >  1:2276994/1‑150 (MQ=255)
                                                                                                                ttttttACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGga                                  <  2:1913097/150‑1 (MQ=255)
                                                                                                                     tACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGc                             <  2:3214242/150‑1 (MQ=255)
                                                                                                                     tACCCGCGTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGc                             <  1:3006673/150‑1 (MQ=255)
                                                                                                                         cgcgTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTc                         >  1:1606553/1‑150 (MQ=255)
                                                                                                                         cgcgTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTc                         <  2:4582253/150‑1 (MQ=255)
                                                                                                                          gcgTTGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCt                        <  2:2232468/150‑1 (MQ=255)
                                                                                                                              tGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCtt                    <  1:1031233/150‑1 (MQ=255)
                                                                                                                              tGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCtt                    >  2:3953493/1‑150 (MQ=255)
                                                                                                                              tGACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTAGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCtt                    >  2:2809002/1‑150 (MQ=255)
                                                                                                                               gACCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTc                   <  2:4742597/150‑1 (MQ=255)
                                                                                                                                aCCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCg                  >  2:309259/1‑150 (MQ=255)
                                                                                                                                aCCATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCg                  >  1:149658/1‑150 (MQ=255)
                                                                                                                                  cATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGAc                >  2:2310735/1‑150 (MQ=255)
                                                                                                                                  cATTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGAc                <  2:1375677/150‑1 (MQ=255)
                                                                                                                                   aTTGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACa               >  1:537273/1‑150 (MQ=255)
                                                                                                                                     tGGCTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACaaa             <  2:4669155/150‑1 (MQ=255)
                                                                                                                                        cTCGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAAttt          <  2:2375976/150‑1 (MQ=255)
                                                                                                                                          cGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCt        <  2:4899488/150‑1 (MQ=255)
                                                                                                                                          cGATGGTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCt        >  1:1837159/1‑150 (MQ=255)
                                                                                                                                              ggTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTACTGCTTCGACAAATTTATGAAt    >  1:2746286/1‑150 (MQ=255)
                                                                                                                                               gTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATc   <  2:2666307/150‑1 (MQ=255)
                                                                                                                                               gTGATCGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATc   <  1:3538790/150‑1 (MQ=255)
                                                                                                                                               gTGATCGTCATCCAAGGTTTCAGTACGACTTTTGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGACGCGACCAATACCGTGTCCGCAATATACACGAAAGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATc   >  1:508576/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCGTCAGAATTTCGCAGCCGTTATCAGTCACCACAATAGTATGCTCATATTGTGCAGACAAGCTGCGATCTTTGGTTTTTACCGTCCAGCCATCTTTCATGGTGCGGATCTCTTTTTTACCCGCGTTGACCATTGGCTCGATGGTGAACGTCATCCCAGGTTTCAGTACGACGTTGGTTTCACGGGAGTCATAGTGCAGCACCTGCGGTTCTTCATGGAAGCCGCGACCAATACCGTGTCCGCAATATTCACGAACGACGGAGAAGCCTTCTGCTTCGACAAATTTCTGAATCG  >  CP009273/185246‑185539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑