Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,953,876 C→A 100% P76P (CCG→CCT)  yecM ← putative metal‑binding enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,953,8760CA98.8% 1081.0 / 2.1 340P76P (CCG→CCT) yecMputative metal‑binding enzyme
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base A (172/163);  total (175/165)
Fisher's exact test for biased strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 340 total aligned reads displayed.

CAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAACCCCAGACGGCGGGA  >  CP009273/1953729‑1954022
                                                                                                                                                   |                                                                                                                                                  
cAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAgg                                                                                                                                                  <  2:4825203/150‑1 (MQ=255)
  gCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAGTAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTc                                                                                                                                                <  2:2581597/150‑1 (MQ=255)
   cGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCt                                                                                                                                               >  2:2164568/1‑150 (MQ=255)
    gTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTg                                                                                                                                              >  2:3781062/1‑150 (MQ=255)
     tttCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGc                                                                                                                                             >  1:3237666/1‑150 (MQ=255)
         tGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCAtt                                                                                                                                         <  2:607514/150‑1 (MQ=255)
         tGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCAtt                                                                                                                                         <  2:2703424/150‑1 (MQ=255)
         tGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCAtt                                                                                                                                         >  2:3587284/1‑150 (MQ=255)
          ggATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTg                                                                                                                                        >  1:3461785/1‑150 (MQ=255)
          ggATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTg                                                                                                                                        >  1:4974051/1‑150 (MQ=255)
          ggATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTg                                                                                                                                        <  2:4138440/150‑1 (MQ=255)
          ggATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTg                                                                                                                                        <  1:1331239/150‑1 (MQ=255)
           gATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGa                                                                                                                                       >  2:1747241/1‑150 (MQ=255)
           gATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGa                                                                                                                                       >  1:4144782/1‑150 (MQ=255)
            aTCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGAt                                                                                                                                      <  2:1711172/150‑1 (MQ=255)
            aTCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGAt                                                                                                                                      <  2:1347052/150‑1 (MQ=255)
            aTCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGAt                                                                                                                                      <  2:279160/150‑1 (MQ=255)
            aTCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGAt                                                                                                                                      >  2:3093774/1‑150 (MQ=255)
              cACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCa                                                                                                                                    <  1:4156770/150‑1 (MQ=255)
               aCCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCat                                                                                                                                   <  1:1644442/150‑1 (MQ=255)
               aCCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCat                                                                                                                                   <  1:3650180/150‑1 (MQ=255)
                 ccGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCatat                                                                                                                                 <  2:1900261/150‑1 (MQ=255)
                  cGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATAtt                                                                                                                                <  2:278893/150‑1 (MQ=255)
                  cGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATAtt                                                                                                                                <  2:864400/150‑1 (MQ=255)
                  cGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATAtt                                                                                                                                >  1:2542676/1‑150 (MQ=255)
                   ggCAGAACGATTTCAATATTTTCCCAACCTTCGTGTGGGTTAAGTTTTTCCCCGGGCCACGTTATCTCCACAATAGAAAACTGCCAGTGCTCAACCTGTACCGGTTCTTGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATAttt                                                                                                                               <  1:1423186/150‑1 (MQ=255)
                   ggCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCTGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATAttt                                                                                                                               >  1:3847755/1‑150 (MQ=255)
                      aGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCt                                                                                                                            >  2:1028817/1‑150 (MQ=255)
                       gAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGAGCATATTTTCTg                                                                                                                           >  2:1151292/1‑150 (MQ=255)
                          cGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACa                                                                                                                        <  2:1280656/150‑1 (MQ=255)
                            aTTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACaaa                                                                                                                      <  1:825047/150‑1 (MQ=255)
                            aTTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACaaa                                                                                                                      >  1:1003049/1‑150 (MQ=255)
                             tttCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACaaaa                                                                                                                     >  1:921722/1‑150 (MQ=255)
                                cAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCt                                                                                                                  >  2:81118/1‑150 (MQ=255)
                                cAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCt                                                                                                                  <  1:1861700/150‑1 (MQ=255)
                                 aaTATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTc                                                                                                                 >  1:4420390/1‑150 (MQ=255)
                                 aaTATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTc                                                                                                                 >  1:2690228/1‑150 (MQ=255)
                                      gTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCcac                                                                                                            >  1:457134/1‑150 (MQ=255)
                                      gTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCcac                                                                                                            >  1:2263984/1‑150 (MQ=255)
                                      gTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCcac                                                                                                            <  1:5266728/150‑1 (MQ=255)
                                         cccAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACt                                                                                                         <  1:2108306/150‑1 (MQ=255)
                                              ccTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCa                                                                                                    >  2:2352474/1‑150 (MQ=255)
                                               cTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCaa                                                                                                   <  2:1522952/150‑1 (MQ=255)
                                               cTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCaa                                                                                                   >  1:2927840/1‑150 (MQ=255)
                                                 tCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAAc                                                                                                 <  2:3939694/150‑1 (MQ=255)
                                                  cGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAAcc                                                                                                <  1:4506785/150‑1 (MQ=255)
                                                    tgtgGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCcg                                                                                              <  1:1094480/150‑1 (MQ=255)
                                                        ggTAACGTTTTTCGCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGAc                                                                                          <  2:834370/150‑1 (MQ=255)
                                                          tAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGc                                                                                        >  1:7924/1‑150 (MQ=255)
                                                          tAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGc                                                                                        >  2:5075526/1‑150 (MQ=255)
                                                             cGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAg                                                                                     >  2:1975243/1‑150 (MQ=255)
                                                                 tttCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGtt                                                                                 >  2:4997091/1‑150 (MQ=255)
                                                                   tCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTca                                                                               >  1:3347513/1‑150 (MQ=255)
                                                                    ccccGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTcag                                                                              >  2:3195992/1‑150 (MQ=255)
                                                                         ggCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTg                                                                         >  2:1798083/1‑150 (MQ=255)
                                                                          gCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTgg                                                                        <  2:4768355/150‑1 (MQ=255)
                                                                           ccACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGc                                                                       <  1:4068202/150‑1 (MQ=255)
                                                                            cccGGTAGCTCCACACTAGAAAACTGCCAGTGCGCACCCTGTCCCGGTTCATGCAATTTAAACAGACACATAGGTCTGCCATTGATCCGCTTTTCTGACAACAGCTCGCCACACGGTTCAAACCCGCGACGCCAGCGTTCAGCAGGGGCg                                                                      >  2:511642/3‑150 (MQ=255)
                                                                                   gCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGAt                                                               >  2:4682469/1‑150 (MQ=255)
                                                                                    cTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTGCAGCAGTGGCGTTTTGATg                                                              >  2:3885646/1‑150 (MQ=255)
                                                                                     tCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATgg                                                             >  2:677015/1‑150 (MQ=255)
                                                                                     tCCACAATAGAAAACTGCCAGAGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTGTGATgg                                                             >  2:1260876/1‑150 (MQ=255)
                                                                                     tCCACAAGAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATgg                                                             <  2:3673409/150‑1 (MQ=255)
                                                                                      ccACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGc                                                            >  2:5272226/1‑150 (MQ=255)
                                                                                      ccACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGc                                                            >  1:4337497/1‑150 (MQ=255)
                                                                                       cacaATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCa                                                           >  1:2048002/1‑150 (MQ=255)
                                                                                       cacaATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCa                                                           >  1:1552646/1‑150 (MQ=255)
                                                                                       cacaATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCa                                                           >  2:3266479/1‑150 (MQ=255)
                                                                                         caATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTATACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAAc                                                         <  2:1843078/150‑1 (MQ=255)
                                                                                         caATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAAc                                                         >  1:1144095/1‑150 (MQ=255)
                                                                                         caATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAAc                                                         <  1:2053799/150‑1 (MQ=255)
                                                                                         caATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAAc                                                         <  1:3782047/150‑1 (MQ=255)
                                                                                          aaTAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACg                                                        >  1:2988511/1‑150 (MQ=255)
                                                                                                       cAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGa                                           >  2:822788/1‑150 (MQ=255)
                                                                                                       cAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGa                                           <  1:3923629/150‑1 (MQ=255)
                                                                                                         gTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCTGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATc                                         <  2:3376958/150‑1 (MQ=255)
                                                                                                         gTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATc                                         <  1:2815333/150‑1 (MQ=255)
                                                                                                          tGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCg                                        <  2:2500184/150‑1 (MQ=255)
                                                                                                           gcgcAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCgg                                       >  2:4902164/1‑150 (MQ=255)
                                                                                                           gcgcAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCgg                                       >  2:2256290/1‑150 (MQ=255)
                                                                                                              cAACCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAg                                    <  1:548050/150‑1 (MQ=255)
                                                                                                                aCCTGTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTc                                  >  2:1639472/1‑150 (MQ=255)
                                                                                                                    gTACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCg                              <  2:3540808/150‑1 (MQ=255)
                                                                                                                     tACCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCgg                             <  2:4420390/150‑1 (MQ=255)
                                                                                                                      aCCGGTTCATGCAATTTAAACAGACAACTAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGa                            <  1:3587787/150‑1 (MQ=255)
                                                                                                                      aCCGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGa                            <  1:4707319/150‑1 (MQ=255)
                                                                                                                       ccGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGACATGTGATCGGCAGTCAGCGGAg                           >  2:2296026/1‑150 (MQ=255)
                                                                                                                       ccGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAg                           >  2:4284575/1‑150 (MQ=255)
                                                                                                                        cGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGt                          >  2:1464570/1‑150 (MQ=255)
                                                                                                                        cGGTTCATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGt                          <  2:742488/150‑1 (MQ=255)
                                                                                                                             cATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGatat                     <  1:636444/150‑1 (MQ=255)
                                                                                                                             cATGCAATTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGatat                     <  1:2722657/150‑1 (MQ=255)
                                                                                                                                  aaTTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAAc                <  2:2228866/150‑1 (MQ=255)
                                                                                                                                   aTTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCTGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAAcc               <  1:1959318/150‑1 (MQ=255)
                                                                                                                                   aTTTAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAAcc               <  1:2352474/150‑1 (MQ=255)
                                                                                                                                      tAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAACCCCa            >  2:3460023/1‑150 (MQ=255)
                                                                                                                                      tAAACAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAACCCCa            <  1:46272/150‑1 (MQ=255)
                                                                                                                                        aaCAGACAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAACCCCAGa          >  1:3024783/1‑150 (MQ=255)
                                                                                                                                              cAAATAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAACCCCAGAcggcgg    >  1:1603441/1‑150 (MQ=255)
                                                                                                                                               aaaTAGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAACCCCAGACGGCggg   >  2:2121282/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCCAACCTTCGTGTGGGTAACGTTTTTCCCCGGGCCACGGTAGCTCCACAATAGAAAACTGCCAGTGCGCAACCTGTACCGGTTCATGCAATTTAAACAGACAAATCGGTCTGCCATTGATCATATTTTCTGACAAAAGCTCGCCACACTGTTCAAACCCGCGACGCCAGCGTTCAGCAGTGGCGTTTTGATGGCAACGCAAAGAAATGTGATCGGCAGTCAGCGGAGTGATATTCAACCCCAGACGGCGGGA  >  CP009273/1953729‑1954022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑