BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,955,401	C→A	59.6%	S361S (TCC→TCA)	argS →	arginyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,955,401	0	C	A	59.6%	98.5 / inf	312	S361S (TCC→TCA)	argS	arginyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base C (73/53); new base A (107/78); total (180/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 315 total aligned reads displayed.

CTCTACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCC  >  CP009273/1955252‑1955547

ctctACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATcc                                                                                                                                                    >  1:118142/1‑150 (MQ=255)

   tACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTa                                                                                                                                                 >  1:992300/1‑150 (MQ=255)

   tACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTa                                                                                                                                                 >  2:2515802/1‑150 (MQ=255)

    acacCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTAc                                                                                                                                                >  2:2166288/1‑150 (MQ=255)

     caccaccacTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTAcc                                                                                                                                               <  1:3459821/150‑1 (MQ=255)

     caccaccacTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTAcc                                                                                                                                               >  2:887809/1‑150 (MQ=255)

     caccaccacTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTAcc                                                                                                                                               >  2:4519801/1‑150 (MQ=255)

     caacaccacTGATATCGCCTGTTGTAAATTTCGTTATTAACCTCTGCATGCCGATCGCGTGGTGTATTACATCGACTCCCGTCAGCATCAACACCTTATGCAGGCATGGGAGATCGTCCTTAATGCAGGCTATGTACCGGAATCAGTAcc                                                                                                                                               <  2:2071363/147‑1 (MQ=255)

      accaccacTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCg                                                                                                                                              >  1:1835689/1‑150 (MQ=255)

      accaccacTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCg                                                                                                                                              >  1:1401504/1‑150 (MQ=255)

       ccaccacTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGACTCAGTACCGc                                                                                                                                             >  2:5013996/1‑150 (MQ=255)

            acTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTCACGGACTCAGTACCGCTGGaa                                                                                                                                        >  2:3755781/1‑150 (MQ=255)

            acTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGaa                                                                                                                                        <  1:2142594/150‑1 (MQ=255)

              tGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAaca                                                                                                                                      <  1:228548/150‑1 (MQ=255)

                atatCGCCAGTGCGAAATATCGATATGAAACACTGCATGCCGATCGCGTGCAGTATTACATCGACTCCCGACAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGTCTATGAACCGTAATCCGTACCGCAGTAAcacc                                                                                                                                    >  2:1367449/1‑150 (MQ=255)

                 tatCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAAcacca                                                                                                                                   >  2:1723461/1‑150 (MQ=255)

                 tatCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAAcacca                                                                                                                                   <  2:4592666/150‑1 (MQ=255)

                     gCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATg                                                                                                                               <  2:3154522/150‑1 (MQ=255)

                       cTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGtt                                                                                                                             >  1:1963398/1‑150 (MQ=255)

                        tgtgCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTc                                                                                                                            <  1:3540554/150‑1 (MQ=255)

                        tgtgCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTc                                                                                                                            <  1:3249689/150‑1 (MQ=255)

                             gAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCat                                                                                                                       >  2:5294485/1‑150 (MQ=255)

                             gAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCat                                                                                                                       >  1:2500560/1‑150 (MQ=255)

                                atatCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCatgat                                                                                                                    >  2:7586/1‑150 (MQ=255)

                                atatCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCatgat                                                                                                                    >  2:3649072/1‑150 (MQ=255)

                                atatCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCatgat                                                                                                                    >  2:325839/1‑150 (MQ=255)

                                atatCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAACAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCatgat                                                                                                                    >  1:2894724/1‑150 (MQ=255)

                                 tatCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCatgatg                                                                                                                   <  1:3440296/150‑1 (MQ=255)

                                         tGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTaa                                                                                                           >  2:3484376/1‑150 (MQ=255)

                                         tGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTaa                                                                                                           >  1:1908367/1‑150 (MQ=255)

                                          gAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTaaa                                                                                                          >  2:4963631/1‑150 (MQ=255)

                                            aaCACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGa                                                                                                        >  1:2476579/1‑150 (MQ=255)

                                            aaCACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGa                                                                                                        >  2:2693261/1‑150 (MQ=255)

                                             acacTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGAc                                                                                                       >  2:2930048/1‑150 (MQ=255)

                                              cacTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACg                                                                                                      <  2:4117695/150‑1 (MQ=255)

                                              cacTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACg                                                                                                      >  1:2601201/1‑150 (MQ=255)

                                                   cATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCaaa                                                                                                 <  1:466145/150‑1 (MQ=255)

                                                   cATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCaaa                                                                                                 >  2:1948387/1‑150 (MQ=255)

                                                   cATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCaaa                                                                                                 >  2:1543478/1‑150 (MQ=255)

                                                    aTGCCGATCGCGTGCTTTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAAc                                                                                                <  1:4070574/150‑1 (MQ=255)

                                                    aTGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTCCCGGAATCCGTACCGCTGGCACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAAc                                                                                                >  2:1443815/1‑150 (MQ=255)

                                                    aTGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAAc                                                                                                <  2:4512779/150‑1 (MQ=255)

                                                     tGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAAcc                                                                                               <  1:2274295/150‑1 (MQ=255)

                                                     tGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAAcc                                                                                               <  1:4188310/150‑1 (MQ=255)

                                                        cGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGtt                                                                                            >  1:3046092/1‑150 (MQ=255)

                                                         gATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTc                                                                                           >  1:4391297/1‑150 (MQ=255)

                                                         gATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTc                                                                                           >  1:1350874/1‑150 (MQ=255)

                                                          aTCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCa                                                                                          <  2:4671675/150‑1 (MQ=255)

                                                            cgcgTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCaaa                                                                                        >  2:657010/1‑150 (MQ=255)

                                                                tGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAAccc                                                                                    >  1:923110/1‑150 (MQ=255)

                                                                    gTATAACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGAATGATGCTGGGTAAAGACGGCAAACCGATCAAAACCcgcgc                                                                                >  2:2398053/1‑150 (MQ=255)

                                                                      aTTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCgg                                                                              >  2:2578904/1‑150 (MQ=255)

                                                                       ttACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCggg                                                                             >  2:3577523/1‑150 (MQ=255)

                                                                              gACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACa                                                                      >  2:1374489/1‑150 (MQ=255)

                                                                                 tCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCACACCGTTCAAAACCCGCGCGGGTGGTACAGTg                                                                   >  1:2395695/1‑150 (MQ=255)

                                                                                  cccGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGa                                                                  >  1:1315169/1‑150 (MQ=255)

                                                                                    cGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGaaa                                                                <  1:1381453/150‑1 (MQ=255)

                                                                                      tCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACt                                                              <  2:44885/150‑1 (MQ=255)

                                                                                       cAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTg                                                             <  2:1613057/150‑1 (MQ=255)

                                                                                       cAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTg                                                             >  1:3698222/1‑150 (MQ=255)

                                                                                          cATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACACTCGCGGGTGGTACAGTGAAACTGGcc                                                          >  2:2463207/1‑150 (MQ=255)

                                                                                             cAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGAt                                                       >  2:4785126/1‑150 (MQ=255)

                                                                                               acacCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATct                                                     <  2:4814349/150‑1 (MQ=255)

                                                                                               acacCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTAACGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATct                                                     <  1:2794740/150‑1 (MQ=255)

                                                                                                cacCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATctg                                                    <  1:4519828/150‑1 (MQ=255)

                                                                                                cacCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATctg                                                    <  1:5227388/150‑1 (MQ=255)

                                                                                                 acCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATctgc                                                   >  2:183739/1‑150 (MQ=255)

                                                                                                  ccTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATctgct                                                  <  2:4219261/150‑1 (MQ=255)

                                                                                                   cTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATctgctg                                                 <  1:401648/150‑1 (MQ=255)

                                                                                                    tGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTgg                                                >  2:2270923/1‑150 (MQ=255)

                                                                                                    tGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGAGCGGGTGGTACAGTGAAACTGGCCGATCTGCTgg                                                >  1:1113804/1‑150 (MQ=255)

                                                                                                    tGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTgg                                                <  2:977487/150‑1 (MQ=255)

                                                                                                     gATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGa                                               >  2:1489972/1‑150 (MQ=255)

                                                                                                           ttcATTGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGTATGAAGc                                         >  2:3613352/3‑150 (MQ=255)

                                                                                                           ggCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGc                                         >  2:781906/1‑150 (MQ=255)

                                                                                                            gCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGcc                                        >  2:3328073/1‑150 (MQ=255)

                                                                                                              aTGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCt                                      <  1:1650610/150‑1 (MQ=255)

                                                                                                               tGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTg                                     <  2:1307330/150‑1 (MQ=255)

                                                                                                                 ggCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGGTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCAGGa                                   >  1:2740867/1‑150 (MQ=255)

                                                                                                                    gATCGTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACg                                >  1:4976866/1‑150 (MQ=255)

                                                                                                                        gTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACCTGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCa                            >  2:511419/1‑150 (MQ=255)

                                                                                                                        gTCCGTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGACCGTGCa                            >  2:3189162/1‑150 (MQ=255)

                                                                                                                            gTAAAGCAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGcc                        <  2:1908367/150‑1 (MQ=255)

                                                                                                                              aaaGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGt                      >  1:2112924/1‑150 (MQ=255)

                                                                                                                              aaaGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGt                      >  2:2843634/1‑150 (MQ=255)

                                                                                                                               aaGCAGGCTATGGACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTc                     <  2:2986064/150‑1 (MQ=255)

                                                                                                                                  cAGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCtgg                  >  1:4824879/1‑150 (MQ=255)

                                                                                                                                   aGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCtggt                 >  1:2683164/1‑150 (MQ=255)

                                                                                                                                   aGGCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCtggt                 <  1:14730/150‑1 (MQ=255)

                                                                                                                                    ggCTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCtggtg                <  1:4901082/150‑1 (MQ=255)

                                                                                                                                      cTATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGc              >  2:3957377/1‑150 (MQ=255)

                                                                                                                                       tATGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCa             <  2:2368373/150‑1 (MQ=255)

                                                                                                                                         tGTACCGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGa           >  1:70517/1‑150 (MQ=255)

                                                                                                                                          gTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGaa          <  2:118142/150‑1 (MQ=255)

                                                                                                                                          gTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGaa          >  1:549113/1‑150 (MQ=255)

                                                                                                                                           tACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGaaa         >  1:1006426/1‑150 (MQ=255)

                                                                                                                                             ccGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAg       <  1:4285661/150‑1 (MQ=255)

                                                                                                                                             ccGGAATCAGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGACGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAg       >  2:3121724/1‑150 (MQ=255)

                                                                                                                                                gAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAAc    >  2:4730066/1‑150 (MQ=255)

                                                                                                                                                  aTCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAAccc  >  1:867396/1‑150 (MQ=255)

CTCTACACCACCACTGATATCGCCTGTGCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGTCAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAATCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTCAAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGTGCACGCCGTCTGGTGGCAGAAAAGAACCC  >  CP009273/1955252‑1955547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`