Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,962,923 C→A 100% M233I (ATG→ATT)  cheR ← chemotaxis regulator, protein‑glutamate methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,962,9230CA99.0% 918.4 / ‑1.3 285M233I (ATG→ATT) cheRchemotaxis regulator, protein‑glutamate methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (130/150);  total (131/154)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 285 total aligned reads displayed.

CGCATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAA  >  CP009273/1962776‑1963069
                                                                                                                                                   |                                                                                                                                                  
cgcATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAAt                                                                                                                                                  <  1:3180736/150‑1 (MQ=255)
cgcATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAAt                                                                                                                                                  <  1:5056170/150‑1 (MQ=255)
    tACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACg                                                                                                                                              >  1:408940/1‑150 (MQ=255)
      cacCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGtt                                                                                                                                            >  1:2899517/1‑150 (MQ=255)
         cGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACg                                                                                                                                         >  2:3123442/1‑150 (MQ=255)
         cGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACg                                                                                                                                         <  2:2327504/150‑1 (MQ=255)
           tCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGAc                                                                                                                                       <  2:2495412/150‑1 (MQ=255)
              gACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACaga                                                                                                                                    >  1:3650485/1‑150 (MQ=255)
              gACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACaga                                                                                                                                    >  2:4427307/1‑150 (MQ=255)
                 cACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACagaaga                                                                                                                                 >  1:554181/1‑150 (MQ=255)
                   cGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATc                                                                                                                               <  2:5130936/150‑1 (MQ=255)
                   cGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATc                                                                                                                               <  1:201352/150‑1 (MQ=255)
                    gCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCg                                                                                                                              >  2:3625870/1‑150 (MQ=255)
                       gCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCAt                                                                                                                           <  2:4024857/150‑1 (MQ=255)
                       gCGTGAAGCGGCGCTCAAGGGGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTTGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCAt                                                                                                                           <  1:2111492/150‑1 (MQ=255)
                        cGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATc                                                                                                                          <  1:3599463/150‑1 (MQ=255)
                         gTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTCGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCa                                                                                                                         >  2:3309861/1‑150 (MQ=255)
                          tGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCaa                                                                                                                        >  1:3067285/1‑150 (MQ=255)
                                ggcgCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGcc                                                                                                                  <  1:2687579/150‑1 (MQ=255)
                                  cgcTCATGGTGGATAATGTTTTCAGTGTGACCCGCAAACTGCAATCCGTGGGGTTTAAGGAGGGTAACAAAGCGGCGCAAAATCTCCTGCTGGGTTGTTTGATCGAAGTAGTTAATGACGTTACGACAGAAGATCGAATCAAACGGcccc                                                                                                                <  1:3333714/150‑1 (MQ=255)
                                      cAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCTGAACAAATCTGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCa                                                                                                            >  1:3736132/1‑150 (MQ=255)
                                      cAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCa                                                                                                            <  1:3869330/150‑1 (MQ=255)
                                       aaGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCAc                                                                                                           <  2:2659301/150‑1 (MQ=255)
                                          gTGGCTAATGTTTTCAGAGTGACCCGCAAACATCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGAAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGgt                                                                                                        <  1:3451223/150‑1 (MQ=255)
                                            ggCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGgtgt                                                                                                      <  2:2856586/150‑1 (MQ=255)
                                              cTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAACGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTAc                                                                                                    <  1:843541/150‑1 (MQ=255)
                                               tAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACt                                                                                                   <  1:2778420/150‑1 (MQ=255)
                                               tAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACt                                                                                                   >  2:2448069/1‑150 (MQ=255)
                                                       tCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGcc                                                                                           >  1:5276938/1‑150 (MQ=255)
                                                         agagTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAg                                                                                         >  1:2484229/1‑150 (MQ=255)
                                                         agagTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAg                                                                                         >  1:3140132/1‑150 (MQ=255)
                                                          gagTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGt                                                                                        <  2:4251324/150‑1 (MQ=255)
                                                          gagTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGt                                                                                        <  1:112268/150‑1 (MQ=255)
                                                            gTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAg                                                                                      <  2:2138658/150‑1 (MQ=255)
                                                             tGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGa                                                                                     >  1:5167287/1‑150 (MQ=255)
                                                              gACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGAt                                                                                    <  1:1937976/150‑1 (MQ=255)
                                                               aCCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGAtt                                                                                   >  2:3038049/1‑150 (MQ=255)
                                                                cccGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTc                                                                                  >  2:1424952/1‑150 (MQ=255)
                                                                 ccGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACACAGCGGCGCAAAATCTCCGGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCa                                                                                 <  2:1988391/150‑1 (MQ=255)
                                                                 ccGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCa                                                                                 <  1:908800/150‑1 (MQ=255)
                                                                   gCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATACTGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGc                                                                               <  2:1881382/150‑1 (MQ=255)
                                                                   gCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGc                                                                               >  2:4401816/1‑150 (MQ=255)
                                                                   gCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGc                                                                               <  2:2553449/150‑1 (MQ=255)
                                                                   gCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGc                                                                               >  1:1851560/1‑150 (MQ=255)
                                                                    cAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCg                                                                              <  2:341225/150‑1 (MQ=255)
                                                                     aaaCAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCgg                                                                             >  1:4422757/1‑150 (MQ=255)
                                                                           cAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCaaa                                                                       >  1:2326801/1‑150 (MQ=255)
                                                                           cAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGAGTACTGTTTCGCCAGTAGATTCAGCGGGGCaaa                                                                       >  2:116743/1‑150 (MQ=255)
                                                                             aTCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAAt                                                                     >  2:1587488/1‑150 (MQ=255)
                                                                                 gTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAAc                                                                 <  1:1325027/150‑1 (MQ=255)
                                                                                   cGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACAt                                                               <  1:4299726/150‑1 (MQ=255)
                                                                                   cGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACAt                                                               >  2:1143797/1‑150 (MQ=255)
                                                                                     ggTTTAAGGAGCGGAACAACGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAg                                                             <  1:3736961/150‑1 (MQ=255)
                                                                                     ggTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAg                                                             >  1:4596841/1‑150 (MQ=255)
                                                                                       tttAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGtt                                                           >  1:4974214/1‑150 (MQ=255)
                                                                                        ttAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTc                                                          <  2:1564284/150‑1 (MQ=255)
                                                                                         tAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGGTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCg                                                         >  2:4631592/1‑150 (MQ=255)
                                                                                          aaGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGc                                                        >  1:1805091/1‑150 (MQ=255)
                                                                                          aaGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGc                                                        >  1:77404/1‑150 (MQ=255)
                                                                                          aaGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGc                                                        >  1:1794186/1‑150 (MQ=255)
                                                                                          aaGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGc                                                        >  2:3876237/1‑150 (MQ=255)
                                                                                           aGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGcc                                                       >  2:868024/1‑150 (MQ=255)
                                                                                             gAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAg                                                     <  2:1022666/150‑1 (MQ=255)
                                                                                                   aaCAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTg                                               <  1:3531224/150‑1 (MQ=255)
                                                                                                     cAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGac                                             <  2:1349341/150‑1 (MQ=255)
                                                                                                      aaaGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGacg                                            <  1:3773751/150‑1 (MQ=255)
                                                                                                          cggcgCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGacgcacg                                        <  2:3470897/150‑1 (MQ=255)
                                                                                                               cAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTAc                                   <  1:2956495/150‑1 (MQ=255)
                                                                                                                aaaaTCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTAcc                                  >  2:5003265/1‑150 (MQ=255)
                                                                                                                 aaaTCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCa                                 <  1:2224488/150‑1 (MQ=255)
                                                                                                                 aaaTCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCa                                 <  2:57416/150‑1 (MQ=255)
                                                                                                                  aaTCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAg                                >  1:1158591/1‑150 (MQ=255)
                                                                                                                  aaTCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAg                                >  1:4779200/1‑150 (MQ=255)
                                                                                                                   aTCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGc                               >  2:3943519/1‑150 (MQ=255)
                                                                                                                   aTCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGc                               <  1:4251180/150‑1 (MQ=255)
                                                                                                                   aTCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGc                               <  1:4379588/150‑1 (MQ=255)
                                                                                                                     ctcCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGccc                             >  2:5275009/1‑150 (MQ=255)
                                                                                                                       ccTGCTGGGTAGTTTGATCGAAGTAGATTATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCtt                           <  1:633240/150‑1 (MQ=255)
                                                                                                                        ctgctgGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTc                          >  2:489791/1‑150 (MQ=255)
                                                                                                                          gctgGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCAt                        <  2:2318664/150‑1 (MQ=255)
                                                                                                                           ctgGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATg                       <  1:1913129/150‑1 (MQ=255)
                                                                                                                            tgGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGc                      >  2:2946538/1‑150 (MQ=255)
                                                                                                                               gTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGc                   <  1:3119008/150‑1 (MQ=255)
                                                                                                                                 aGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGccc                 >  2:734456/1‑150 (MQ=255)
                                                                                                                                   tttGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCg               <  1:2163674/150‑1 (MQ=255)
                                                                                                                                    ttGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGt              <  2:3194443/150‑1 (MQ=255)
                                                                                                                                     tGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTc             >  1:2725373/1‑150 (MQ=255)
                                                                                                                                      gATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTcc            <  1:4015352/150‑1 (MQ=255)
                                                                                                                                      gATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTcc            <  2:474132/150‑1 (MQ=255)
                                                                                                                                       aTCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTccc           >  1:2611307/1‑150 (MQ=255)
                                                                                                                                        tCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCt          <  1:440053/150‑1 (MQ=255)
                                                                                                                                        tCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCt          <  2:3736481/150‑1 (MQ=255)
                                                                                                                                          gAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCg        <  2:1666991/150‑1 (MQ=255)
                                                                                                                                          gAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCg        >  2:2835559/1‑150 (MQ=255)
                                                                                                                                          gAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCg        <  2:2799024/150‑1 (MQ=255)
                                                                                                                                            aGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCa      <  2:4828592/150‑1 (MQ=255)
                                                                                                                                            aGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCa      >  1:2535631/1‑150 (MQ=255)
                                                                                                                                             gTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCAt     <  1:1056865/150‑1 (MQ=255)
                                                                                                                                               aGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGa   >  1:1549934/1‑150 (MQ=255)
                                                                                                                                               aGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGa   >  1:1728353/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGCATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATGAA  >  CP009273/1962776‑1963069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑