Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,021,954 C→A 65.9% E298D (GAG→GAT)  yedI ← DUF808 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,021,9540CA65.9% 211.6 / inf 288E298D (GAG→GAT) yedIDUF808 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (52/46);  new base A (104/86);  total (156/132)
Fisher's exact test for biased strand distribution p-value = 8.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 295 total aligned reads displayed.

GCCGCCCTCTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATG  >  CP009273/2021807‑2022100
                                                                                                                                                   |                                                                                                                                                  
 ccgccCTCTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTctct                                                                                                                                                 <  1:1423562/150‑1 (MQ=255)
 ccgccCTCTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTatct                                                                                                                                                 <  2:3406436/150‑1 (MQ=255)
 ccgccCTCTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTatct                                                                                                                                                 <  2:1024583/150‑1 (MQ=255)
          ttAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTc                                                                                                                                        >  1:3200260/1‑150 (MQ=255)
           tAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCa                                                                                                                                       <  1:605683/150‑1 (MQ=255)
            aaCCGTAACTTGCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCAc                                                                                                                                      >  1:2731799/1‑150 (MQ=255)
            aaCCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCAc                                                                                                                                      <  1:3873501/150‑1 (MQ=255)
               cGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTcc                                                                                                                                   >  2:308768/1‑150 (MQ=255)
               cGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTcc                                                                                                                                   <  2:887118/150‑1 (MQ=255)
               cGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCC‑TTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTccc                                                                                                                                  >  1:4500603/1‑150 (MQ=255)
                 tAACGTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCa                                                                                                                                 <  1:2081116/150‑1 (MQ=255)
                    cTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGca                                                                                                                              >  2:1654531/1‑150 (MQ=255)
                     ttCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGcac                                                                                                                             >  2:1738311/1‑150 (MQ=255)
                        cTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGcaccac                                                                                                                          >  1:1448212/1‑150 (MQ=255)
                          cACTAACCATCCCGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGa                                                                                                                        <  1:4213476/150‑1 (MQ=255)
                          cACTAACCATAAAGCTCATTCTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGa                                                                                                                        >  2:241547/1‑150 (MQ=255)
                           aCTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGAt                                                                                                                       >  1:2479234/1‑150 (MQ=255)
                           aCTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGAt                                                                                                                       >  1:4350497/1‑150 (MQ=255)
                            cTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATg                                                                                                                      <  2:4184677/150‑1 (MQ=255)
                              aaCCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATgcc                                                                                                                    >  2:4912533/1‑150 (MQ=255)
                              aaCCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATgcc                                                                                                                    >  2:3020076/1‑150 (MQ=255)
                              aaCCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATgcc                                                                                                                    >  2:3749456/1‑150 (MQ=255)
                               aCCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATgccg                                                                                                                   <  2:2638507/150‑1 (MQ=255)
                                 cATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATgccgcc                                                                                                                 >  2:2526721/1‑150 (MQ=255)
                                   tAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCga                                                                                                               <  2:1382324/150‑1 (MQ=255)
                                   tAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCga                                                                                                               >  1:3276272/1‑150 (MQ=255)
                                    aaaGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCgat                                                                                                              >  2:2867307/1‑150 (MQ=255)
                                    aaaGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCgat                                                                                                              <  1:4163310/150‑1 (MQ=255)
                                       gCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCgatgat                                                                                                           <  1:3521811/150‑1 (MQ=255)
                                       gCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCgatgat                                                                                                           <  1:2539955/150‑1 (MQ=255)
                                       gCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCgatgat                                                                                                           <  1:1321157/150‑1 (MQ=255)
                                         tCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATaa                                                                                                         <  2:2704960/150‑1 (MQ=255)
                                           aTACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAAt                                                                                                       <  2:3369975/150‑1 (MQ=255)
                                           aTACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAAt                                                                                                       <  1:1601009/150‑1 (MQ=255)
                                           aTACTCGCCTCCTGTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAAt                                                                                                       <  1:1023503/150‑1 (MQ=255)
                                             aCTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATcc                                                                                                     <  1:308768/150‑1 (MQ=255)
                                             aCTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATcc                                                                                                     >  2:3694848/1‑150 (MQ=255)
                                             aCTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATcc                                                                                                     <  2:3933199/150‑1 (MQ=255)
                                                cGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAg                                                                                                  <  1:2970302/150‑1 (MQ=255)
                                                   ctcctTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAAt                                                                                               <  1:1945173/150‑1 (MQ=255)
                                                    tcctTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATc                                                                                              >  2:2307946/1‑150 (MQ=255)
                                                      ctTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAg                                                                                            >  1:4858871/1‑150 (MQ=255)
                                                        tttttCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGAt                                                                                          <  1:1200418/150‑1 (MQ=255)
                                                             cGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTaaa                                                                                     <  1:5062813/150‑1 (MQ=255)
                                                             cGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTaaa                                                                                     <  1:694662/150‑1 (MQ=255)
                                                              gtgTGAAACTTGTTCACATTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTaaaa                                                                                    <  2:4444696/148‑1 (MQ=255)
                                                              gAGTGAATCTTGTTCACCTTAGTTGAAGATGGCGTATTTTGCATAATAGCCAGTGCGAGTATTTATGTGCCTGACCGCGCATTTTATCTTCGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTaaaa                                                                                    <  2:5030794/150‑1 (MQ=255)
                                                               aGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAAc                                                                                   >  2:1906229/1‑150 (MQ=255)
                                                               aGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAAc                                                                                   <  1:1660377/150‑1 (MQ=255)
                                                               aGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAAc                                                                                   >  2:5202179/1‑150 (MQ=255)
                                                                gTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTaaaaaa                                                                                  >  1:2147634/1‑148 (MQ=255)
                                                                gTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACa                                                                                  <  2:632476/150‑1 (MQ=255)
                                                                gTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACa                                                                                  >  1:1988995/1‑150 (MQ=255)
                                                                 tGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAg                                                                                 <  1:599877/150‑1 (MQ=255)
                                                                 tGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAg                                                                                 >  1:4408704/1‑150 (MQ=255)
                                                                 tGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAg                                                                                 >  2:809521/1‑150 (MQ=255)
                                                                   aaaCTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAGTCCAAGAATCAGATTTAAAACAGTc                                                                               >  2:1158616/1‑150 (MQ=255)
                                                                     aCTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCgg                                                                             >  2:3855369/1‑150 (MQ=255)
                                                                      cTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGt                                                                            <  2:2051798/150‑1 (MQ=255)
                                                                         gTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAat                                                                         <  1:1546214/150‑1 (MQ=255)
                                                                             aCCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATatca                                                                     >  2:2121882/1‑150 (MQ=255)
                                                                              ccTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATatcat                                                                    <  1:176221/150‑1 (MQ=255)
                                                                               cTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATatcatc                                                                   <  1:3917828/150‑1 (MQ=255)
                                                                               cTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATatcatc                                                                   >  1:3774564/1‑150 (MQ=255)
                                                                               cTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATatcatc                                                                   <  1:289376/150‑1 (MQ=255)
                                                                                ttAGTTGAAGATGGCGATTTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGACTCAGATTTAAAACAGTCGGTAATATCATCg                                                                  <  1:815816/150‑1 (MQ=255)
                                                                                 tAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGTTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGc                                                                 >  2:3827344/1‑150 (MQ=255)
                                                                                  aGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGcc                                                                <  2:4358772/150‑1 (MQ=255)
                                                                                    ttGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCac                                                              >  1:1694468/1‑150 (MQ=255)
                                                                                     tGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCact                                                             >  1:2861088/1‑150 (MQ=255)
                                                                                      gAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCacta                                                            >  1:3173791/1‑150 (MQ=255)
                                                                                       aaGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCactac                                                           >  2:1940822/1‑150 (MQ=255)
                                                                                       aaGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCactac                                                           <  2:4527867/150‑1 (MQ=255)
                                                                                        aGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCactact                                                          <  2:3607540/150‑1 (MQ=255)
                                                                                          aTGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGc                                                        <  1:2662022/150‑1 (MQ=255)
                                                                                                 aTTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTtgc                                                 >  1:3720448/1‑150 (MQ=255)
                                                                                                 aTTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTtgc                                                 <  2:2797134/150‑1 (MQ=255)
                                                                                                      gCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGccc                                            >  2:3390903/1‑150 (MQ=255)
                                                                                                       cATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCg                                           >  1:3663984/1‑150 (MQ=255)
                                                                                                       cATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCg                                           >  2:2411730/1‑150 (MQ=255)
                                                                                                          aataGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCg                                        >  1:5209071/1‑150 (MQ=255)
                                                                                                            taGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGaa                                      >  2:3527156/1‑150 (MQ=255)
                                                                                                            taGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTTCTGCCCGGCGaa                                      >  1:4577790/1‑150 (MQ=255)
                                                                                                             aGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGaaa                                     >  2:1305889/1‑150 (MQ=255)
                                                                                                              gCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAAt                                    >  1:259045/1‑150 (MQ=255)
                                                                                                              gCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAAt                                    <  1:4675568/150‑1 (MQ=255)
                                                                                                                   tGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCa                               <  2:5329500/150‑1 (MQ=255)
                                                                                                                      gAGTATTAATGTGCCTGACCTCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCTGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATg                            >  1:4459189/1‑150 (MQ=255)
                                                                                                                          aTTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGt                        >  1:1981214/1‑150 (MQ=255)
                                                                                                                          aTTAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGt                        >  1:1815501/1‑150 (MQ=255)
                                                                                                                           ttAATGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTg                       >  2:3135526/1‑150 (MQ=255)
                                                                                                                             aaTGTTCCTGAGCGCGCATTTTATCTACGGCTTTCACTCACTTCACAACTATGCCGCCGATTCTAAATCAAATAATCAGATTTAAAACCGTCTTTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGAt                     <  1:95135/150‑1 (MQ=255)
                                                                                                                             aaTGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGAt                     <  1:2291700/150‑1 (MQ=255)
                                                                                                                             aaTGTGCCTGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGAt                     <  2:2163169/150‑1 (MQ=255)
                                                                                                                               tgtgCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGc                   >  2:672720/1‑150 (MQ=255)
                                                                                                                                gtgCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCa                  <  1:508367/150‑1 (MQ=255)
                                                                                                                                   ccTCACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTAAATGGCGTGAAGCAgcg               >  2:1488543/1‑150 (MQ=255)
                                                                                                                                     tGACCGCGCATTTTATCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAgcggc             >  2:3060692/1‑150 (MQ=255)
                                                                                                                                       aCCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGgcgc           >  1:4595542/1‑150 (MQ=255)
                                                                                                                                               ttttATCTTCGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATATATCCAAGAATCAGTTTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCAt   <  2:4826079/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCCGCCCTCTTTAACCGTAACTTCCTCACTAACCATAAAGCTCATACTCGCCTCCTTTTTTCGAGTGAAACTTGTTCACCTTAGTTGAAGATGGCGAATTTTGCATAATAGCCAGTGCGAGTATTAATGTGCCTGACCGCGCATTTTCTCTACGGCTTTCACTCCCAGCACCACGATGCCGCCGATGATAAATCCAAGAATCAGATTTAAAACAGTCGGTAATATCATCGCCACTACTGCACTTTGCTGCCCGGCGAAATGTTCAATGGCGTGATGCAGCGGCGCAATTCCATG  >  CP009273/2021807‑2022100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑