Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,040,890 C→A 25.9% G824G (GGC→GGA)  yeeJ → putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,040,8900CA25.9% 783.4 / 221.2 358G824G (GGC→GGA) yeeJputative adhesin
Reads supporting (aligned to +/- strand):  ref base C (143/122);  new base A (52/41);  total (195/163)
Fisher's exact test for biased strand distribution p-value = 8.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.07e-01

Only 100 of 361 total aligned reads displayed.

CATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAA  >  CP009273/2040741‑2041039
                                                                                                                                                     |                                                                                                                                                     
cATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGc                                                                                                                                                       <  1:1205856/150‑1 (MQ=255)
cATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGc                                                                                                                                                       <  1:1044194/150‑1 (MQ=255)
   aCCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTg                                                                                                                                                    >  2:5176739/1‑150 (MQ=255)
     cGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGaa                                                                                                                                                  <  2:3206711/150‑1 (MQ=255)
        cACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGaaaca                                                                                                                                               >  1:2284143/1‑150 (MQ=255)
           gTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGaaacaaac                                                                                                                                            >  1:3333594/1‑150 (MQ=255)
               gCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTa                                                                                                                                        <  2:2906509/150‑1 (MQ=255)
                 ggTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAAGGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAAt                                                                                                                                      <  2:4186136/150‑1 (MQ=255)
                  gTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATc                                                                                                                                     <  2:651378/150‑1 (MQ=255)
                     ttAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTc                                                                                                                                  <  2:1766340/150‑1 (MQ=255)
                       aaGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAg                                                                                                                                <  1:1378939/150‑1 (MQ=255)
                         gCGGATCGGCAACGTCCTTCAACAATCACAACACCGCAAAAACGGTTGTTAATGGTCTGGCGTCTTTTGATCTGATAAGTAGTAAGCAGGAAGACAACAAGGTTGAAGTCACCCTTGTATATGGCGTGAAACAAACGTTAATCGTCAGtt                                                                                                                              <  1:882745/150‑1 (MQ=255)
                          cGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGttt                                                                                                                             >  2:4213389/1‑150 (MQ=255)
                           ggATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGtttt                                                                                                                            >  2:4567865/1‑150 (MQ=255)
                           ggATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGtttt                                                                                                                            >  1:2909995/1‑150 (MQ=255)
                             aTCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGt                                                                                                                          >  1:1831040/1‑150 (MQ=255)
                                 gCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGc                                                                                                                      >  2:1619899/1‑150 (MQ=255)
                                    aCTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGAc                                                                                                                   >  2:2707303/1‑150 (MQ=255)
                                     cttccttcAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACt                                                                                                                  >  2:1318394/1‑150 (MQ=255)
                                        ccttcAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGa                                                                                                               <  2:2362702/150‑1 (MQ=255)
                                             aacaaTCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACt                                                                                                          <  2:3818214/150‑1 (MQ=255)
                                             aacaaTCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTCTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACt                                                                                                          <  1:1388818/150‑1 (MQ=255)
                                              acaaTCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTg                                                                                                         >  1:2332872/1‑150 (MQ=255)
                                               caaTCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTgc                                                                                                        >  1:1500224/1‑150 (MQ=255)
                                                aaTCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGATAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTgcg                                                                                                       >  2:2200198/1‑150 (MQ=255)
                                                aaTCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTgcg                                                                                                       >  1:1619066/1‑150 (MQ=255)
                                                 aTCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTgcgc                                                                                                      >  1:4438220/1‑150 (MQ=255)
                                                   cAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAg                                                                                                    >  1:3745041/1‑150 (MQ=255)
                                                      aaCACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGtt                                                                                                 <  2:3475544/150‑1 (MQ=255)
                                                      aaCACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGtt                                                                                                 >  1:4489889/1‑150 (MQ=255)
                                                      aaCACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGtt                                                                                                 <  1:2433434/150‑1 (MQ=255)
                                                       acacCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTg                                                                                                <  2:435130/150‑1 (MQ=255)
                                                       acacCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTg                                                                                                >  1:3496557/1‑150 (MQ=255)
                                                           cGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCATGTTGATCt                                                                                            <  2:5126834/150‑1 (MQ=255)
                                                           cGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCt                                                                                            <  2:3089589/150‑1 (MQ=255)
                                                              aaaaaCGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCa                                                                                         >  1:5126017/1‑150 (MQ=255)
                                                              aaaaaCGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCa                                                                                         >  2:1013486/1‑150 (MQ=255)
                                                               aaaaCGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAg                                                                                        <  1:2560729/150‑1 (MQ=255)
                                                               aaaaCGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAg                                                                                        <  2:2671503/150‑1 (MQ=255)
                                                                  aCGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAg                                                                                     <  2:3973699/150‑1 (MQ=255)
                                                                   cGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGt                                                                                    <  1:4763816/150‑1 (MQ=255)
                                                                    ggATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGATAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTc                                                                                   <  2:3726442/150‑1 (MQ=255)
                                                                    ggATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTc                                                                                   >  2:1825263/1‑150 (MQ=255)
                                                                    ggATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTc                                                                                   <  2:1201288/150‑1 (MQ=255)
                                                                    ggATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTc                                                                                   >  2:3145052/1‑150 (MQ=255)
                                                                      aTGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGa                                                                                 >  2:4911487/1‑150 (MQ=255)
                                                                        gTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGaaa                                                                               >  2:2811125/1‑150 (MQ=255)
                                                                         ttAATGGTCTGGATACTTTTGATCTGAAAAGTAGTAAGCAGTAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGaaaa                                                                              <  1:2804991/150‑1 (MQ=255)
                                                                           aaTGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAAt                                                                            >  1:3588710/1‑150 (MQ=255)
                                                                           aaTGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAAt                                                                            <  1:5176808/150‑1 (MQ=255)
                                                                                tCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGt                                                                       >  1:3187556/1‑150 (MQ=255)
                                                                                 cTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTg                                                                      >  2:4976734/1‑150 (MQ=255)
                                                                                 cTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTg                                                                      >  2:2573220/1‑150 (MQ=255)
                                                                                     cGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTg                                                                  <  1:3290730/150‑1 (MQ=255)
                                                                                     cGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTg                                                                  <  1:4181065/150‑1 (MQ=255)
                                                                                      gACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGc                                                                 >  2:2140168/1‑150 (MQ=255)
                                                                                       aCTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCt                                                                <  1:45646/150‑1 (MQ=255)
                                                                                       aCTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCt                                                                >  2:2862388/1‑150 (MQ=255)
                                                                                        cTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTg                                                               >  1:3011360/1‑150 (MQ=255)
                                                                                        cTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTg                                                               >  2:803266/1‑150 (MQ=255)
                                                                                           ttGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACg                                                            <  1:146520/150‑1 (MQ=255)
                                                                                            tGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACgg                                                           >  1:2007119/1‑150 (MQ=255)
                                                                                             gATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGc                                                          >  1:2713148/1‑150 (MQ=255)
                                                                                              aTCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCa                                                         <  2:1708441/150‑1 (MQ=255)
                                                                                                        tagtaAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGt                                               >  1:5222631/1‑150 (MQ=255)
                                                                                                         agtaAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTc                                              >  1:2680206/1‑150 (MQ=255)
                                                                                                           taAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTcac                                            <  2:3377866/150‑1 (MQ=255)
                                                                                                              gCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAAt                                         >  2:1136771/1‑150 (MQ=255)
                                                                                                               cAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATg                                        >  1:3162152/1‑150 (MQ=255)
                                                                                                                aGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGACGTCGAAAAATGAAGTGGGTGCTGCCGGCAATGACAGCGTCACAATGa                                       >  2:4656616/1‑150 (MQ=255)
                                                                                                                 ggAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGAc                                      <  2:197194/150‑1 (MQ=255)
                                                                                                                  gAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGAcc                                     <  1:4284136/150‑1 (MQ=255)
                                                                                                                  gAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGAcc                                     >  1:969050/1‑150 (MQ=255)
                                                                                                                  gAAGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGAcc                                     >  1:503881/1‑150 (MQ=255)
                                                                                                                   aaGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACcg                                    >  1:2967737/1‑150 (MQ=255)
                                                                                                                    aGACAACACGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACcgc                                   >  2:4824601/1‑150 (MQ=255)
                                                                                                                       caacaCGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGAc                                <  1:3964047/150‑1 (MQ=255)
                                                                                                                       caacaCGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGAc                                >  2:3111832/1‑150 (MQ=255)
                                                                                                                         acacGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCg                              >  2:3651876/1‑150 (MQ=255)
                                                                                                                         acacGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCg                              >  1:2848716/1‑150 (MQ=255)
                                                                                                                         acacGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTTACGGCAATGACAGCGTCACAATGACCGCTACCg                              <  2:2521691/150‑1 (MQ=255)
                                                                                                                          cacGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGt                             >  1:3452870/1‑150 (MQ=255)
                                                                                                                            cGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTcc                           <  1:492647/150‑1 (MQ=255)
                                                                                                                            cGGTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTcc                           <  1:841362/150‑1 (MQ=255)
                                                                                                                             ggTTGAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCg                          >  1:645821/1‑150 (MQ=255)
                                                                                                                              gTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCgg                         >  1:4187553/1‑150 (MQ=255)
                                                                                                                                 gAAGTCACCCTTGAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGAt                      <  2:3772731/150‑1 (MQ=255)
                                                                                                                                  aaGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATg                     <  1:1691696/150‑1 (MQ=255)
                                                                                                                                        cccTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGTCCGTCCGGGATGCAAAAg               >  2:4154482/1‑150 (MQ=255)
                                                                                                                                         ccTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAgg              <  2:2043079/150‑1 (MQ=255)
                                                                                                                                         ccTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAgg              <  2:3260123/150‑1 (MQ=255)
                                                                                                                                          cTTGAAAATGGCGTGACACAAACGTTTATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGc             <  1:803142/150‑1 (MQ=255)
                                                                                                                                             gAAAATGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAAc          <  1:3817313/150‑1 (MQ=255)
                                                                                                                                              aaaaTGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAAcc         >  1:3096954/1‑150 (MQ=255)
                                                                                                                                               aaaTGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCt        >  2:943049/1‑150 (MQ=255)
                                                                                                                                                aaTGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTg       <  2:3333594/150‑1 (MQ=255)
                                                                                                                                                  tGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCt     <  1:4363917/150‑1 (MQ=255)
                                                                                                                                                  tGGAGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCt     >  1:2234686/1‑150 (MQ=255)
                                                                                                                                                    gCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCa   <  2:2247915/150‑1 (MQ=255)
                                                                                                                                                     cGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCaa  >  1:3639793/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CATACCGTCACGTTTGCGGTATTAAGCGGATCGGCAACTTCCTTCAACAATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAA  >  CP009273/2040741‑2041039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑