BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,043,643	T→A	16.9%	L1742Q (CTG→CAG)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,043,643	0	T	A	16.9%	832.0 / 139.7	332	L1742Q (CTG→CAG)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand): ref base T (129/144); new base A (26/30); total (155/177)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 346 total aligned reads displayed.

TAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGACCAGTTTG  >  CP009273/2043494‑2043792

tAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCctctct                                                                                                                                                       >  1:1569830/1‑150 (MQ=255)

     gCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAg                                                                                                                                                  >  1:400447/1‑150 (MQ=255)

      cAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGa                                                                                                                                                 >  1:4818600/1‑150 (MQ=255)

            ggTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAAcc                                                                                                                                           >  2:485267/1‑150 (MQ=255)

              tAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTa                                                                                                                                         <  2:3265239/150‑1 (MQ=255)

              tAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAAAAGTTTCAGCCTCTCAGGCAGATAAACCTa                                                                                                                                         <  1:4606163/150‑1 (MQ=255)

               aGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTAc                                                                                                                                        >  1:2355827/1‑150 (MQ=255)

               aGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTAc                                                                                                                                        <  2:1439547/150‑1 (MQ=255)

                gAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACt                                                                                                                                       >  1:5077578/1‑150 (MQ=255)

                 aaGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTg                                                                                                                                      >  1:904109/1‑150 (MQ=255)

                   ggCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGaa                                                                                                                                    <  1:2165405/150‑1 (MQ=255)

                    gCATAAAAGTTAATTTCCGCGGAACCTCCGTCTCGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAg                                                                                                                                   <  2:574919/150‑1 (MQ=255)

                     cATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACAGAAGt                                                                                                                                  >  1:3657948/1‑150 (MQ=255)

                     cATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATACACCTACTGAAGt                                                                                                                                  >  2:5077274/1‑150 (MQ=255)

                      aTAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTc                                                                                                                                 >  1:785314/1‑150 (MQ=255)

                       tAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCa                                                                                                                                <  2:1685973/150‑1 (MQ=255)

                         aaaGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCAtc                                                                                                                              >  2:2295441/1‑150 (MQ=255)

                          aaGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCAtct                                                                                                                             <  1:3764798/150‑1 (MQ=255)

                           aGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCAtctc                                                                                                                            >  1:4166455/1‑150 (MQ=255)

                           aGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCAtctc                                                                                                                            <  2:821488/150‑1 (MQ=255)

                                aTTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGAt                                                                                                                       >  2:1373140/1‑150 (MQ=255)

                                  ttCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTa                                                                                                                     <  2:771050/150‑1 (MQ=255)

                                   tCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCTCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTAc                                                                                                                    <  2:4347488/150‑1 (MQ=255)

                                   tCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTAc                                                                                                                    <  1:3620939/150‑1 (MQ=255)

                                     cgcgGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTg                                                                                                                  <  1:342408/150‑1 (MQ=255)

                                       cgGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGaa                                                                                                                <  1:2964278/150‑1 (MQ=255)

                                       cgGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGaa                                                                                                                >  1:210516/1‑150 (MQ=255)

                                        ggAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAAt                                                                                                               <  2:3076967/150‑1 (MQ=255)

                                         gAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATg                                                                                                              >  2:3684709/1‑150 (MQ=255)

                                         gAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATg                                                                                                              <  1:4346797/150‑1 (MQ=255)

                                            ccTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCa                                                                                                           >  1:5255745/1‑150 (MQ=255)

                                             cTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAg                                                                                                          <  1:2674531/150‑1 (MQ=255)

                                              tCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGt                                                                                                         <  2:2481622/150‑1 (MQ=255)

                                              accGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGt                                                                                                         <  2:3970464/149‑1 (MQ=255)

                                                 gTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCa                                                                                                      >  2:1168436/1‑150 (MQ=255)

                                                     cGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATg                                                                                                  <  1:3221925/150‑1 (MQ=255)

                                                      gCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGt                                                                                                 >  2:4803041/1‑150 (MQ=255)

                                                        tAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTa                                                                                               >  1:1325873/1‑150 (MQ=255)

                                                          agcagcACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAAt                                                                                             <  1:617252/150‑1 (MQ=255)

                                                            cagcaCCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTc                                                                                           <  2:4790628/150‑1 (MQ=255)

                                                             agcaCCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCt                                                                                          >  2:2938275/1‑150 (MQ=255)

                                                             agcaCCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCt                                                                                          <  1:4942390/150‑1 (MQ=255)

                                                             agcaCCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCt                                                                                          >  1:4786700/1‑150 (MQ=255)

                                                             agcaCCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCt                                                                                          >  2:252845/1‑150 (MQ=255)

                                                                aCCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGcg                                                                                       >  1:3844613/1‑150 (MQ=255)

                                                                aCCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGcg                                                                                       >  1:3509168/1‑150 (MQ=255)

                                                                   aGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGcgacg                                                                                    >  2:4704106/1‑150 (MQ=255)

                                                                   aGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGcgacg                                                                                    <  2:1787314/150‑1 (MQ=255)

                                                                   aGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGcgacg                                                                                    >  2:2146489/1‑150 (MQ=255)

                                                                    gCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGcgacga                                                                                   <  2:858686/150‑1 (MQ=255)

                                                                    gCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGcgacga                                                                                   <  1:244519/150‑1 (MQ=255)

                                                                      gTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGAtt                                                                                 >  1:3463163/1‑150 (MQ=255)

                                                                        tGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTAc                                                                               <  1:394372/150‑1 (MQ=255)

                                                                         gAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTAcc                                                                              >  1:4379499/1‑150 (MQ=255)

                                                                             cGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTc                                                                          <  1:463315/150‑1 (MQ=255)

                                                                              ggATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCt                                                                         >  2:2894838/1‑150 (MQ=255)

                                                                                      ggggTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATAc                                                                 >  2:3189684/1‑150 (MQ=255)

                                                                                      ggggTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATAc                                                                 >  2:4231041/1‑150 (MQ=255)

                                                                                             cGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAgg                                                          <  1:954683/150‑1 (MQ=255)

                                                                                               cTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTc                                                        <  2:2806336/150‑1 (MQ=255)

                                                                                                tGATATTCTTGTGACAAGCACCGTGGTCGGACTGAATACAGTTTCAGCCTCTCCGGCAGATAAACCTACTGAAGTCATCTCGCGTTTACTGATTGCCAGTGCAGATGTTACTTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCa                                                       <  1:1147572/150‑1 (MQ=255)

                                                                                                  aaaTTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAgg                                                     >  1:4080286/1‑150 (MQ=255)

                                                                                                   aaTTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGt                                                    <  2:503549/150‑1 (MQ=255)

                                                                                                   aaTTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGt                                                    <  1:3111914/150‑1 (MQ=255)

                                                                                                     ttCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTaa                                                  <  2:1751253/150‑1 (MQ=255)

                                                                                                       cTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATg                                                <  1:1777448/150‑1 (MQ=255)

                                                                                                       cTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATg                                                <  1:1873163/150‑1 (MQ=255)

                                                                                                        ttGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATgg                                               <  1:354730/150‑1 (MQ=255)

                                                                                                            gACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGc                                           >  2:3458447/1‑150 (MQ=255)

                                                                                                            gACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGc                                           >  1:4465738/1‑150 (MQ=255)

                                                                                                            gACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGc                                           >  1:3023471/1‑150 (MQ=255)

                                                                                                            gACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGc                                           >  1:1448439/1‑150 (MQ=255)

                                                                                                              cAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGcac                                         <  2:1532026/150‑1 (MQ=255)

                                                                                                               aaGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGcaca                                        >  2:3720613/1‑150 (MQ=255)

                                                                                                                   aCCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGAc                                    >  1:748942/1‑150 (MQ=255)

                                                                                                                       aGGTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAg                                >  2:4107698/1‑150 (MQ=255)

                                                                                                                        ggTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGc                               <  2:4478827/150‑1 (MQ=255)

                                                                                                                        ggTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGACGGTCAGGTAATGGTCGCACAAGACGTAGc                               >  2:405588/1‑150 (MQ=255)

                                                                                                                        ggTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGAGTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGc                               <  1:3721437/150‑1 (MQ=255)

                                                                                                                         gTCGGACTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCa                              <  2:3292318/150‑1 (MQ=255)

                                                                                                                           cGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGTCGATTACAAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCTCAAGACGTAGCAGt                            <  1:102364/150‑1 (MQ=255)

                                                                                                                              aCTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTaa                         <  2:1259047/150‑1 (MQ=255)

                                                                                                                              aCTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTaa                         >  2:1294359/1‑150 (MQ=255)

                                                                                                                              aCTGAAAACAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTaa                         >  1:2028698/1‑150 (MQ=255)

                                                                                                                                tGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAg                       <  1:1845084/150‑1 (MQ=255)

                                                                                                                                tGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAg                       >  1:1710483/1‑150 (MQ=255)

                                                                                                                                 gAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGc                      <  1:5139738/150‑1 (MQ=255)

                                                                                                                                 gAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGc                      >  2:5296964/1‑150 (MQ=255)

                                                                                                                                   aaaCAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTc                    >  1:1957599/1‑150 (MQ=255)

                                                                                                                                    aaCAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCa                   >  1:1210921/1‑150 (MQ=255)

                                                                                                                                     aCAGTTTCAGCCTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCAc                  <  2:2082123/150‑1 (MQ=255)

                                                                                                                                          ttCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTaa             <  2:2751673/150‑1 (MQ=255)

                                                                                                                                            cAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACg           <  2:1569830/150‑1 (MQ=255)

                                                                                                                                            cAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACg           >  2:1908885/1‑150 (MQ=255)

                                                                                                                                            cAGCCTCTCTGGCAGATAAACCAACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACg           <  2:3917911/150‑1 (MQ=255)

                                                                                                                                               ccTCTCAGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGAcc        <  2:1055691/150‑1 (MQ=255)

                                                                                                                                                  ctctGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGACCAGt     >  1:2528773/1‑150 (MQ=255)

                                                                                                                                                   tctGGCATATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCTGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGACCAGtt    <  1:1601753/150‑1 (MQ=255)

                                                                                                                                                     tGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGACCAGTTTg  <  2:705090/150‑1 (MQ=255)

                                                                                                                                                     tGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGACCAGTTTg  <  2:3784060/150‑1 (MQ=255)

TAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGAACCTCCGTCACGCTAAGCAGCACCAGCGTTGAAACGGATGATCGGGGTTTCGCTGAAATTCTTGTGACAAGCACCGAGGTCGGACTGAAAACAGTTTCAGCCTCTCTGGCAGATAAACCTACTGAAGTCATCTCGCGATTACTGAATGCCAGTGCAGATGTTAATTCTGCGACGATTACCAGTCTGGAGATACCGGAAGGTCAGGTAATGGTCGCACAAGACGTAGCAGTTAAAGCTCACGTTAACGACCAGTTTG  >  CP009273/2043494‑2043792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`