Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,086,004 C→A 100% A42A (GCC→GCA)  hisC → histidinol‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,086,0040CA97.8% 843.2 / 7.9 270A42A (GCC→GCA) hisChistidinol‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (1/5);  new base A (127/136);  total (128/142)
Fisher's exact test for biased strand distribution p-value = 2.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 270 total aligned reads displayed.

TTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTGCT  >  CP009273/2085857‑2086150
                                                                                                                                                   |                                                                                                                                                  
ttAACGCCCTTAAGGAGCAATCATGAGCACCGTGAATATTACCGATTTCGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGt                                                                                                                                                  <  1:915646/150‑1 (MQ=255)
ttAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGt                                                                                                                                                  <  2:1412280/150‑1 (MQ=255)
  aaCGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTgg                                                                                                                                                >  2:758286/1‑150 (MQ=255)
    cGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAg                                                                                                                                              >  1:2685574/1‑150 (MQ=255)
     gCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGt                                                                                                                                             >  1:3147029/1‑150 (MQ=255)
        cTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTc                                                                                                                                          >  2:2821034/1‑150 (MQ=255)
           aaGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGc                                                                                                                                       >  2:2471394/1‑150 (MQ=255)
                gcaagcaTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACt                                                                                                                                  <  2:697319/150‑1 (MQ=255)
                   agcaTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAg                                                                                                                               <  1:2111000/150‑1 (MQ=255)
                   agcaTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAg                                                                                                                               <  1:512439/150‑1 (MQ=255)
                    gcaTGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGc                                                                                                                              <  2:3112028/150‑1 (MQ=255)
                       tGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCaaa                                                                                                                           <  2:4041965/150‑1 (MQ=255)
                        gAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAAc                                                                                                                          <  2:1913209/150‑1 (MQ=255)
                        gAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAAc                                                                                                                          >  2:2279803/1‑150 (MQ=255)
                        gAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAAc                                                                                                                          <  1:4675529/150‑1 (MQ=255)
                           cACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCt                                                                                                                       >  1:2640436/1‑150 (MQ=255)
                            aCCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTc                                                                                                                      >  2:4595140/1‑150 (MQ=255)
                            aCCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTc                                                                                                                      >  2:4942562/1‑150 (MQ=255)
                                tGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAAcc                                                                                                                  >  2:1742790/1‑150 (MQ=255)
                                 gACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCg                                                                                                                 <  2:5123255/150‑1 (MQ=255)
                                 gACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCg                                                                                                                 <  1:3586257/150‑1 (MQ=255)
                                   cTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCt                                                                                                               >  2:4476327/1‑150 (MQ=255)
                                     aTTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTAc                                                                                                             <  1:830044/150‑1 (MQ=255)
                                     aTTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTAc                                                                                                             >  2:4195236/1‑150 (MQ=255)
                                        aCCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCg                                                                                                          >  1:320672/1‑150 (MQ=255)
                                        aCCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCg                                                                                                          >  1:5129690/1‑150 (MQ=255)
                                        aCCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCg                                                                                                          <  1:3638574/150‑1 (MQ=255)
                                        aCCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCg                                                                                                          <  2:2704722/150‑1 (MQ=255)
                                            aTTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAAt                                                                                                      >  1:5281408/1‑150 (MQ=255)
                                            aTTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAAt                                                                                                      >  1:726654/1‑150 (MQ=255)
                                            aTTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAAt                                                                                                      <  1:1417215/150‑1 (MQ=255)
                                              ttAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGc                                                                                                    >  1:1023360/1‑150 (MQ=255)
                                               tAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGcc                                                                                                   >  2:3815040/1‑150 (MQ=255)
                                                aGCGCGTGAAAACGTCCGCACCCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCCCTGCAGTGGAGTTTCAGCTTACTCAGCACACGCTCAACCGCTACCCGGCATGCCa                                                                                                  >  2:3019720/1‑150 (MQ=255)
                                                aGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCa                                                                                                  >  2:4332779/1‑150 (MQ=255)
                                                aGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCa                                                                                                  <  1:4855841/150‑1 (MQ=255)
                                                aGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCa                                                                                                  >  2:502943/1‑150 (MQ=255)
                                                  cgcgTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGc                                                                                                <  2:527110/150‑1 (MQ=255)
                                                   gcgTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGcc                                                                                               >  1:4771595/1‑150 (MQ=255)
                                                     gTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGa                                                                                             >  1:2165401/1‑150 (MQ=255)
                                                       gAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGaaa                                                                                           >  1:1548970/1‑150 (MQ=255)
                                                        aaaaCGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAg                                                                                          >  2:5165151/1‑150 (MQ=255)
                                                        aaaaCGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAg                                                                                          >  2:3179334/1‑150 (MQ=255)
                                                        aaaaCGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAg                                                                                          <  2:1096250/150‑1 (MQ=255)
                                                        aaaaCGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAg                                                                                          >  2:2520956/1‑150 (MQ=255)
                                                         aaaCGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGc                                                                                         <  2:923907/150‑1 (MQ=255)
                                                          aaCGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCg                                                                                        <  1:996619/150‑1 (MQ=255)
                                                            cGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGt                                                                                      <  1:3827912/150‑1 (MQ=255)
                                                            cGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGt                                                                                      <  1:2962544/150‑1 (MQ=255)
                                                            cGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGt                                                                                      <  1:1446342/150‑1 (MQ=255)
                                                             gTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTg                                                                                     >  2:133351/1‑150 (MQ=255)
                                                              tCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGa                                                                                    >  1:1767218/1‑150 (MQ=255)
                                                                cGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGAtt                                                                                  <  1:255889/150‑1 (MQ=255)
                                                                 gCAACGTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTTACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTg                                                                                 <  2:1953818/150‑1 (MQ=255)
                                                                 gCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTg                                                                                 <  1:4122954/150‑1 (MQ=255)
                                                                 gCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTg                                                                                 <  2:3978244/150‑1 (MQ=255)
                                                                  cAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGa                                                                                >  2:3784611/1‑150 (MQ=255)
                                                                     ccTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGaaaa                                                                             >  2:85029/1‑150 (MQ=255)
                                                                       tGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAATGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAAtt                                                                           >  2:2165286/1‑150 (MQ=255)
                                                                         aCGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTAc                                                                         <  1:2567268/150‑1 (MQ=255)
                                                                              gTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCa                                                                    >  1:4069806/1‑150 (MQ=255)
                                                                                aTCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAat                                                                  >  2:982750/1‑150 (MQ=255)
                                                                                    gTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGc                                                              <  2:2304782/150‑1 (MQ=255)
                                                                                    gTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGc                                                              <  2:5299157/150‑1 (MQ=255)
                                                                                      cGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAg                                                            <  1:4598051/150‑1 (MQ=255)
                                                                                      cGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAg                                                            <  1:4973056/150‑1 (MQ=255)
                                                                                       ggCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAgg                                                           >  1:2424797/1‑150 (MQ=255)
                                                                                       ggCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAgg                                                           <  1:3695294/150‑1 (MQ=255)
                                                                                        gcgcGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGc                                                          <  2:2669135/150‑1 (MQ=255)
                                                                                         cgcgTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCg                                                         <  2:4107823/150‑1 (MQ=255)
                                                                                          gcgTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGt                                                        <  2:2729859/150‑1 (MQ=255)
                                                                                            gtcgtcTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTaa                                                      >  2:2067393/1‑150 (MQ=255)
                                                                                             tcgtcTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTaaa                                                     >  2:324339/1‑150 (MQ=255)
                                                                                               gtcTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAAc                                                   >  1:4230000/1‑150 (MQ=255)
                                                                                                tcTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAAcc                                                  <  1:2921564/150‑1 (MQ=255)
                                                                                                  tGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCgg                                                >  1:4341909/1‑150 (MQ=255)
                                                                                                       ggTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAg                                           >  2:1588316/1‑150 (MQ=255)
                                                                                                          aaCGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTg                                        >  1:1439244/1‑150 (MQ=255)
                                                                                                          aaCGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTg                                        >  1:409634/1‑150 (MQ=255)
                                                                                                           aCGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGc                                       <  1:2497707/150‑1 (MQ=255)
                                                                                                                  tGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAg                                >  2:3439225/1‑150 (MQ=255)
                                                                                                                  tGTCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAg                                >  2:3377330/1‑150 (MQ=255)
                                                                                                                    tCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGcc                              <  2:1537102/150‑1 (MQ=255)
                                                                                                                    tCTGGCTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGcc                              <  2:4642258/150‑1 (MQ=255)
                                                                                                                      tGGCTGAACGCCAACGAATA‑CCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTg                           >  2:497356/1‑150 (MQ=255)
                                                                                                                         cTGAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGgc                         >  1:200342/1‑150 (MQ=255)
                                                                                                                           gAACGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGgcgc                       >  2:1195489/1‑150 (MQ=255)
                                                                                                                              cGCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGa                    >  2:4753120/1‑150 (MQ=255)
                                                                                                                               gCCAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGAc                   <  1:1121880/150‑1 (MQ=255)
                                                                                                                                 cAACGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGa                 >  2:1006478/1‑150 (MQ=255)
                                                                                                                                  aaCGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGaa                <  1:4278665/150‑1 (MQ=255)
                                                                                                                                    cGAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAgg              <  2:4413714/150‑1 (MQ=255)
                                                                                                                                     gAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGt             >  1:3365170/1‑150 (MQ=255)
                                                                                                                                     gAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGt             >  2:1669373/1‑150 (MQ=255)
                                                                                                                                     gAATACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGt             >  1:4885845/1‑150 (MQ=255)
                                                                                                                                      aaTACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTa            >  2:3635840/1‑150 (MQ=255)
                                                                                                                                       aTACCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTAt           <  2:2311968/150‑1 (MQ=255)
                                                                                                                                         aCCCCACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTg         >  1:4557524/1‑150 (MQ=255)
                                                                                                                                             cACTGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAAct     <  1:170054/150‑1 (MQ=255)
                                                                                                                                                tGCAGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAGGGTATTGAActgct  >  1:3122386/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTAACGCCCTTAAGGAGCAAGCATGAGCACCGTGACTATTACCGATTTAGCGCGTGAAAACGTCCGCAACCTGACGCCGTATCAGTCGGCGCGTCGTCTGGGCGGTAACGGCGATGTCTGGCTGAACGCCAACGAATACCCCACTGCCGTGGAGTTTCAGCTTACTCAGCAAACGCTCAACCGCTACCCGGAATGCCAGCCGAAAGCGGTGATTGAAAATTACGCGCAATATGCAGGCGTAAAACCGGAGCAGGTGCTGGTCAGCCGTGGCGCGGACGAAGGTATTGAACTGCT  >  CP009273/2085857‑2086150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑