Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,093,612 C→A 67.1% E380* (GAA→TAA)  gnd ← 6‑phosphogluconate dehydrogenase, decarboxylating

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,093,6120CA67.1% 258.5 / inf 310E380* (GAA→TAA) gnd6‑phosphogluconate dehydrogenase, decarboxylating
Reads supporting (aligned to +/- strand):  ref base C (57/45);  new base A (108/100);  total (165/145)
Fisher's exact test for biased strand distribution p-value = 5.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 310 total aligned reads displayed.

CGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAA  >  CP009273/2093465‑2093757
                                                                                                                                                   |                                                                                                                                                 
cGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCgg                                                                                                                                                 >  1:1227075/1‑150 (MQ=255)
   aaTAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCAt                                                                                                                                              >  1:1860730/1‑150 (MQ=255)
    aTAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATa                                                                                                                                             <  2:4577628/150‑1 (MQ=255)
     tAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATaa                                                                                                                                            <  1:4261094/150‑1 (MQ=255)
         cAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCAt                                                                                                                                        <  2:1175374/150‑1 (MQ=255)
         cAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCAt                                                                                                                                        >  1:760568/1‑150 (MQ=255)
               cTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGa                                                                                                                                  <  1:328780/150‑1 (MQ=255)
                tGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATACGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGAt                                                                                                                                 <  2:2880044/150‑1 (MQ=255)
                tGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGAt                                                                                                                                 <  1:4938841/150‑1 (MQ=255)
                tGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGAt                                                                                                                                 >  1:533556/1‑150 (MQ=255)
                 gCGGAGAAGGTCGGAACCTGAATACCGTTCTGTACTGCATAAGCATCGACATCACGCAGCGACTTCTGGTAGTCATAGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGAtt                                                                                                                                <  1:404727/150‑1 (MQ=255)
                  cGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGAttt                                                                                                                               >  2:4849361/1‑150 (MQ=255)
                   ggAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGAtttt                                                                                                                              >  2:3949933/1‑150 (MQ=255)
                   ggAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGAtttt                                                                                                                              >  2:2307930/1‑150 (MQ=255)
                   ggAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGAtttt                                                                                                                              <  2:4454449/150‑1 (MQ=255)
                     agaAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCt                                                                                                                            <  2:1290244/150‑1 (MQ=255)
                      gaAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTg                                                                                                                           >  1:196849/1‑150 (MQ=255)
                      gaAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTg                                                                                                                           <  1:3450845/150‑1 (MQ=255)
                        aGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCa                                                                                                                         <  2:1713964/150‑1 (MQ=255)
                              gAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACt                                                                                                                   <  1:726299/150‑1 (MQ=255)
                              gAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACt                                                                                                                   <  2:740059/150‑1 (MQ=255)
                                aCCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTgc                                                                                                                 <  1:3974310/150‑1 (MQ=255)
                                   ggAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCa                                                                                                              >  2:4375047/1‑150 (MQ=255)
                                    gAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCAc                                                                                                             <  1:2912206/150‑1 (MQ=255)
                                      aTACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACgg                                                                                                           >  1:838649/1‑150 (MQ=255)
                                         ccGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGgatg                                                                                                        <  2:2096757/150‑1 (MQ=255)
                                         ccGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGgatg                                                                                                        <  1:1556902/150‑1 (MQ=255)
                                          cGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGgatga                                                                                                       <  2:3057288/150‑1 (MQ=255)
                                          cGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGgatga                                                                                                       >  2:4743728/1‑150 (MQ=255)
                                           gTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGgatgat                                                                                                      <  2:5045972/150‑1 (MQ=255)
                                           gTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGgatgat                                                                                                      <  2:4235114/150‑1 (MQ=255)
                                                gTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGcagc                                                                                                 <  2:262579/150‑1 (MQ=255)
                                                gTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGcagc                                                                                                 >  2:4188160/1‑150 (MQ=255)
                                                  aCTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGcagcca                                                                                               <  1:960952/150‑1 (MQ=255)
                                                   cTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGcagccag                                                                                              <  1:5192491/150‑1 (MQ=255)
                                                     gCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCa                                                                                            >  2:1005404/1‑150 (MQ=255)
                                                       aTAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACg                                                                                          <  1:1579937/150‑1 (MQ=255)
                                                        tAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACgg                                                                                         >  1:4847931/1‑150 (MQ=255)
                                                        tAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACgg                                                                                         >  1:4967569/1‑150 (MQ=255)
                                                         aaGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGa                                                                                        <  2:2559281/150‑1 (MQ=255)
                                                               tgACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTTAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCt                                                                                  >  1:59167/2‑150 (MQ=255)
                                                                gACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCtt                                                                                 >  2:910265/1‑150 (MQ=255)
                                                                 aCATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTc                                                                                <  2:2478887/150‑1 (MQ=255)
                                                                  cATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTcg                                                                               <  2:2009709/150‑1 (MQ=255)
                                                                   aTCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTcgc                                                                              <  2:3228123/150‑1 (MQ=255)
                                                                    tCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTcgcg                                                                             >  1:3468776/1‑150 (MQ=255)
                                                                    tCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTcgcg                                                                             <  2:533556/150‑1 (MQ=255)
                                                                       cGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGAtt                                                                          <  2:561056/150‑1 (MQ=255)
                                                                        gCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCTGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGAttt                                                                         <  2:3611738/150‑1 (MQ=255)
                                                                        gCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGAttt                                                                         <  1:2708890/150‑1 (MQ=255)
                                                                         cAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTc                                                                        <  2:3330711/150‑1 (MQ=255)
                                                                         cAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTc                                                                        <  1:2477501/150‑1 (MQ=255)
                                                                         cAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTc                                                                        >  1:4620466/1‑150 (MQ=255)
                                                                         cAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGAATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTc                                                                        >  2:3223716/1‑150 (MQ=255)
                                                                            cgcCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGcc                                                                     <  2:1400985/150‑1 (MQ=255)
                                                                              ccTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGt                                                                   >  2:583659/1‑150 (MQ=255)
                                                                                 gctgGTAGTCATCGGCATTTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTGTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGt                                                                <  2:3440756/150‑1 (MQ=255)
                                                                                 gctgGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGt                                                                <  1:1657588/150‑1 (MQ=255)
                                                                                 gctgGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGt                                                                <  1:1509458/150‑1 (MQ=255)
                                                                                    ggTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCa                                                             >  2:4912769/1‑150 (MQ=255)
                                                                                         tCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATcc                                                        <  2:1227075/150‑1 (MQ=255)
                                                                                         tCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATcc                                                        <  1:3172395/150‑1 (MQ=255)
                                                                                          cATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATccc                                                       >  1:3622297/1‑150 (MQ=255)
                                                                                            tCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAg                                                     <  1:1526785/150‑1 (MQ=255)
                                                                                            tCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAg                                                     >  2:4793910/1‑150 (MQ=255)
                                                                                             cGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGt                                                    >  1:2051142/1‑150 (MQ=255)
                                                                                              ggCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGtt                                                   >  1:5250580/1‑150 (MQ=255)
                                                                                               gCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTg                                                  <  2:1528584/150‑1 (MQ=255)
                                                                                                cAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGt                                                 >  2:2270222/1‑150 (MQ=255)
                                                                                                   tttGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTAct                                              <  1:3492500/150‑1 (MQ=255)
                                                                                                        ttGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCa                                         <  1:3862995/150‑1 (MQ=255)
                                                                                                            aGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacg                                     <  1:3949933/150‑1 (MQ=255)
                                                                                                            aGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacg                                     >  1:2117298/1‑150 (MQ=255)
                                                                                                               aCGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacgcag                                  <  1:3229216/150‑1 (MQ=255)
                                                                                                                cGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacgcagc                                 >  2:1093463/1‑150 (MQ=255)
                                                                                                                cGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacgcagc                                 <  1:444189/150‑1 (MQ=255)
                                                                                                                cGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacgcagc                                 >  2:4196164/1‑150 (MQ=255)
                                                                                                                  gAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacgcagcac                               <  1:645827/150‑1 (MQ=255)
                                                                                                                     ccAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacgcagcacgca                            >  1:1463391/1‑150 (MQ=255)
                                                                                                                      cAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacgcagcacgcag                           <  1:1767383/150‑1 (MQ=255)
                                                                                                                      cAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacgcagcacgcag                           >  1:991684/1‑150 (MQ=255)
                                                                                                                       aGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacgcagcacgcagc                          >  1:382501/1‑150 (MQ=255)
                                                                                                                       aGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacgcagcacgcagc                          >  2:4128510/1‑150 (MQ=255)
                                                                                                                        gCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCt                         <  2:181535/150‑1 (MQ=255)
                                                                                                                        gCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCt                         <  2:654805/150‑1 (MQ=255)
                                                                                                                         cAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTg                        >  1:4232002/1‑150 (MQ=255)
                                                                                                                         cAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTg                        >  1:4141079/1‑150 (MQ=255)
                                                                                                                          aaCAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTga                       >  1:2412389/1‑150 (MQ=255)
                                                                                                                          aaCAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTga                       >  2:2104641/1‑150 (MQ=255)
                                                                                                                            cAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTgaga                     >  1:1050180/1‑150 (MQ=255)
                                                                                                                               gTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGaa                  <  2:4069626/150‑1 (MQ=255)
                                                                                                                                ttAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAg                 >  1:2594897/1‑150 (MQ=255)
                                                                                                                                  aGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGcc               <  1:1008720/150‑1 (MQ=255)
                                                                                                                                    cGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCt             >  2:2219332/1‑150 (MQ=255)
                                                                                                                                     gATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTg            <  2:5206692/150‑1 (MQ=255)
                                                                                                                                      aTCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTgg           >  2:3940318/1‑150 (MQ=255)
                                                                                                                                      aTCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTgg           >  1:4520647/1‑150 (MQ=255)
                                                                                                                                         tgtgGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCg        >  2:4295492/1‑150 (MQ=255)
                                                                                                                                          gtgGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGt       >  1:3119033/1‑150 (MQ=255)
                                                                                                                                              aTTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGa   >  1:3731775/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAA  >  CP009273/2093465‑2093757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑