Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,110,346 G→A 100% T88I (ACC→ATC)  wcaL ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,110,3460GA99.3% 968.0 / ‑3.1 292T88I (ACC→ATC) wcaLputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (138/152);  total (139/153)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 294 total aligned reads displayed.

GGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGTGTGTCGCCT  >  CP009273/2110200‑2110492
                                                                                                                                                  |                                                                                                                                                  
ggAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGatat                                                                                                                                                 >  1:3191403/1‑150 (MQ=255)
ggAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGatat                                                                                                                                                 >  1:849147/1‑150 (MQ=255)
 gAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATAtt                                                                                                                                                <  2:4711765/150‑1 (MQ=255)
  aGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATAttt                                                                                                                                               <  2:1820763/150‑1 (MQ=255)
   gTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATAtttt                                                                                                                                              <  1:420748/150‑1 (MQ=255)
   gTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATAtttt                                                                                                                                              >  2:79017/1‑150 (MQ=255)
   gTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATAtttt                                                                                                                                              <  2:4596075/150‑1 (MQ=255)
    ttttGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGGTAttttt                                                                                                                                             <  1:4418816/150‑1 (MQ=255)
    ttttGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATAttttt                                                                                                                                             <  2:2382657/150‑1 (MQ=255)
         ctgcGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGAt                                                                                                                                        >  2:4776289/1‑150 (MQ=255)
           gaggTAACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGa                                                                                                                                      >  2:63090/3‑150 (MQ=255)
            cGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGaa                                                                                                                                     >  2:1178043/1‑150 (MQ=255)
             tgTTACCCCCGCGGTACCATAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCATTTGGCAGACAAAATCAGGGTCCTCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAAt                                                                                                                                    <  2:3282534/149‑1 (MQ=255)
             ggTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAAt                                                                                                                                    <  2:1312395/150‑1 (MQ=255)
             ggTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAAt                                                                                                                                    <  2:4086677/150‑1 (MQ=255)
             ggTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAAt                                                                                                                                    <  2:4179668/150‑1 (MQ=255)
             ggTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAAt                                                                                                                                    <  2:2493795/150‑1 (MQ=255)
             ggTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATAAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAAt                                                                                                                                    >  2:2180093/1‑150 (MQ=255)
               ttACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGc                                                                                                                                  >  1:4568824/1‑150 (MQ=255)
                   ccccGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCcgcg                                                                                                                              <  1:408905/150‑1 (MQ=255)
                   ccccGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGAAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCcgcg                                                                                                                              >  1:988366/1‑150 (MQ=255)
                         gggACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGt                                                                                                                        >  1:2514857/1‑150 (MQ=255)
                          ggACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTc                                                                                                                       <  2:204230/150‑1 (MQ=255)
                          ggACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGAGCCTGCCAGATATTTTTACTATTAATGCCGCGCAAGGTc                                                                                                                       >  1:1092755/1‑150 (MQ=255)
                           gACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTct                                                                                                                      <  2:1423392/150‑1 (MQ=255)
                           gACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTct                                                                                                                      <  1:2892225/150‑1 (MQ=255)
                           gACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTct                                                                                                                      <  1:2302113/150‑1 (MQ=255)
                             ccAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTctgg                                                                                                                    >  2:3507496/1‑150 (MQ=255)
                               aaaaTGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTctggct                                                                                                                  <  2:2926520/150‑1 (MQ=255)
                                 aaTGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATAGTAACGATGAATACCGCGCCAGGTctggcagg                                                                                                                >  2:3540958/1‑150 (MQ=255)
                                   tGAGCGATGAACACATCGGCGCGAAACGGTGTTGCTACCTGGCCGCAAATGGCAGAAAAAATCAGGTTCCGCGACTCTGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTggctggct                                                                                                              <  1:4983316/150‑1 (MQ=255)
                                     aGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCg                                                                                                            >  1:4355145/1‑150 (MQ=255)
                                       cGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGt                                                                                                          >  1:5207741/1‑150 (MQ=255)
                                       cGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGAAAATGGCAGACAAAATAAGGTTCCGCGACTCGTAACCATATCTTTTGATGTTGAGAGACTGCAAGATATTATTCCGATGAATGACTCGCAAGGTATGTCTTGATCGGt                                                                                                          >  2:4137397/1‑150 (MQ=255)
                                        gATGAACACATGGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTg                                                                                                         >  1:2767712/1‑150 (MQ=255)
                                            aaCACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGgcgc                                                                                                     >  1:2930356/1‑150 (MQ=255)
                                               acaTCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGt                                                                                                  >  2:1366928/1‑150 (MQ=255)
                                               acaTCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTATTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGt                                                                                                  >  1:2413767/1‑150 (MQ=255)
                                                  tCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAACATCAGGGTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTc                                                                                               <  2:2683856/150‑1 (MQ=255)
                                                    ggCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGc                                                                                             >  1:4892600/1‑150 (MQ=255)
                                                         gAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACtt                                                                                        >  1:3562573/1‑150 (MQ=255)
                                                         gAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACtt                                                                                        >  2:5266614/1‑150 (MQ=255)
                                                          aaaCGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCCAGGTCTGGCTGGCTCGGTGGCGCGGGTGCGCCACtgt                                                                                       >  2:4816085/1‑148 (MQ=255)
                                                          aaaCGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACttt                                                                                       <  1:4715671/150‑1 (MQ=255)
                                                          aaaCGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACttt                                                                                       >  1:3613455/1‑150 (MQ=255)
                                                           aaCGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTg                                                                                      >  2:3990865/1‑150 (MQ=255)
                                                           aaCGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTCTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTg                                                                                      >  2:740111/1‑150 (MQ=255)
                                                                  ttGCGACCTGTCAGCAAATGGCAGACAAAATCAGGGTCCGCGACTCGGCACCATTGCGTGTGAGGTTGAGCGCATGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAg                                                                               <  2:2098392/150‑1 (MQ=255)
                                                                   tGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAgg                                                                              <  1:3358827/150‑1 (MQ=255)
                                                                      gACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTc                                                                           <  1:3077016/150‑1 (MQ=255)
                                                                       aCCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTGTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCg                                                                          >  1:3842673/1‑150 (MQ=255)
                                                                          tGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTcc                                                                       >  2:2468151/1‑150 (MQ=255)
                                                                           ggCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCt                                                                      <  1:1178043/150‑1 (MQ=255)
                                                                            gCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTg                                                                     >  2:5086841/1‑150 (MQ=255)
                                                                             ccGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGt                                                                    <  2:80510/150‑1 (MQ=255)
                                                                                   aTGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAg                                                              >  2:4433342/1‑150 (MQ=255)
                                                                                    tGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGc                                                             <  2:4753323/150‑1 (MQ=255)
                                                                                     ggCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCg                                                            <  2:1506396/150‑1 (MQ=255)
                                                                                     ggCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCg                                                            >  1:4523019/1‑150 (MQ=255)
                                                                                      gCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCgg                                                           >  1:3738047/1‑150 (MQ=255)
                                                                                        aGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGt                                                         >  1:1541295/1‑150 (MQ=255)
                                                                                        aGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGt                                                         >  1:2585819/1‑150 (MQ=255)
                                                                                         gACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCACGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGtt                                                        >  2:192597/1‑150 (MQ=255)
                                                                                             aaaTCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTgg                                                    <  2:2032337/150‑1 (MQ=255)
                                                                                             aaaTCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTgg                                                    <  1:2534757/150‑1 (MQ=255)
                                                                                             aaaTCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTgg                                                    <  1:2534794/150‑1 (MQ=255)
                                                                                                   gggtCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCaa                                              <  2:4334423/147‑1 (MQ=255)
                                                                                                   ggTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCaa                                              <  2:1675494/150‑1 (MQ=255)
                                                                                                     ttCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAgg                                            >  2:3656979/1‑150 (MQ=255)
                                                                                                           gACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTAt                                      <  1:677882/150‑1 (MQ=255)
                                                                                                            aCTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTAtt                                     >  2:4906137/1‑150 (MQ=255)
                                                                                                            aCTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTAtt                                     <  1:3499308/150‑1 (MQ=255)
                                                                                                            aCTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCACGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTAtt                                     <  2:2547164/150‑1 (MQ=255)
                                                                                                              tCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTc                                   <  2:3444/150‑1 (MQ=255)
                                                                                                               cGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCg                                  <  1:2079075/150‑1 (MQ=255)
                                                                                                                  cACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTcc                               >  2:851050/1‑150 (MQ=255)
                                                                                                                  cACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTcc                               <  1:1814264/150‑1 (MQ=255)
                                                                                                                  cACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTcc                               >  1:598022/1‑150 (MQ=255)
                                                                                                                  cACCATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTcc                               <  1:3962063/150‑1 (MQ=255)
                                                                                                                    ccATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCAt                             <  2:4523019/150‑1 (MQ=255)
                                                                                                                    ccATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCAt                             <  1:2800092/150‑1 (MQ=255)
                                                                                                                     cATAGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATg                            <  2:1062092/150‑1 (MQ=255)
                                                                                                                        aGCGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCcg                         >  1:1954534/1‑150 (MQ=255)
                                                                                                                          cGTTTGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCcgcg                       <  1:4745290/150‑1 (MQ=255)
                                                                                                                             ttGAGGTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTgg                    <  1:3387956/150‑1 (MQ=255)
                                                                                                                                 ggTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGgtgt                >  2:381407/1‑150 (MQ=255)
                                                                                                                                 ggTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGgtgt                <  1:5097361/150‑1 (MQ=255)
                                                                                                                                  gTTGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGtt               <  1:1252860/150‑1 (MQ=255)
                                                                                                                                   ttGAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGttt              >  2:3110658/1‑150 (MQ=255)
                                                                                                                                     gAGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtg            >  1:4596994/1‑150 (MQ=255)
                                                                                                                                      aGCGCCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgt           >  1:2569103/1‑150 (MQ=255)
                                                                                                                                       gcgcCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCTGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgtg          <  1:4460327/150‑1 (MQ=255)
                                                                                                                                        cgcCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgtgt         <  1:3761875/150‑1 (MQ=255)
                                                                                                                                        cgcCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgtgt         >  2:5205226/1‑150 (MQ=255)
                                                                                                                                         gcCTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTtgtgtg        >  2:156515/1‑150 (MQ=255)
                                                                                                                                          ccTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTgtgtgt       <  1:63090/150‑1 (MQ=255)
                                                                                                                                          ccTGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTgtgtgt       >  2:10885/1‑150 (MQ=255)
                                                                                                                                            tGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGTGTGTCg     <  1:1832561/150‑1 (MQ=255)
                                                                                                                                            tGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGTGTGTCg     >  1:1515959/1‑150 (MQ=255)
                                                                                                                                            tGCCAGATATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGTGTGTCg     >  2:2864801/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAACGGTGTTGCGACCTGGCCGCAAATGGCAGACAAAATCAGGTTCCGCGACTCGGCACCATAGCGTTTGAGGTTGAGCGCCTGCCAGGTATTTTTACGATGAATGCCGCGCAAGGTCTGGCTGGCTCGGTGGCGCAGTTTCGCCACTTTGCCCGTAGGTTCGTCCTGTAACCAGCGGGTTCTGGCAGCAAGGTTGTATTTCGTCCATGCCGCGTGGGTGTTTTGTGTGTCGCCT  >  CP009273/2110200‑2110492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑