Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 201,962 A→T 100% D117V (GAT→GTT)  dnaE → DNA polymerase III alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273201,9620AT97.4% 964.0 / 9.8 314D117V (GAT→GTT) dnaEDNA polymerase III alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (6/3);  new base T (141/163);  total (147/167)
Fisher's exact test for biased strand distribution p-value = 3.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 324 total aligned reads displayed.

CAGATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCGC  >  CP009273/201815‑202107
                                                                                                                                                   |                                                                                                                                                 
cAGATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCatcgttc                                                                                                                                                 <  1:4502606/150‑1 (MQ=255)
cAGATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCatcgttc                                                                                                                                                 <  1:3609628/150‑1 (MQ=255)
 aGATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCatcgttcg                                                                                                                                                >  2:4304163/1‑150 (MQ=255)
  gATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTcgc                                                                                                                                               >  1:4087969/1‑150 (MQ=255)
       tAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTAACAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTg                                                                                                                                          <  2:5026451/150‑1 (MQ=255)
        aaCGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACttg                                                                                                                                         <  2:252625/150‑3 (MQ=255)
          cGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCt                                                                                                                                       <  1:1492383/150‑1 (MQ=255)
          cGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCt                                                                                                                                       <  1:3721375/150‑1 (MQ=255)
           gTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCtt                                                                                                                                      >  2:2212840/1‑150 (MQ=255)
           gTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCtt                                                                                                                                      >  1:2600418/1‑150 (MQ=255)
            tCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTa                                                                                                                                     <  2:4801510/150‑1 (MQ=255)
             ccAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTAt                                                                                                                                    >  2:4113117/1‑150 (MQ=255)
              cAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATc                                                                                                                                   <  2:864049/150‑1 (MQ=255)
                    gACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTa                                                                                                                             <  2:4273252/150‑1 (MQ=255)
                    gACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTa                                                                                                                             >  1:475589/1‑150 (MQ=255)
                           tgGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAg                                                                                                                      >  1:1190395/1‑150 (MQ=255)
                            gggTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAgg                                                                                                                     >  2:3442639/1‑150 (MQ=255)
                             ggTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAggg                                                                                                                    >  2:2633067/1‑150 (MQ=255)
                              gTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTCCGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGt                                                                                                                   >  2:2389936/1‑150 (MQ=255)
                              gTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGt                                                                                                                   >  2:12586/1‑150 (MQ=255)
                               tgatgaGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGtt                                                                                                                  <  2:2070920/150‑1 (MQ=255)
                                gatgttttAACCCACCTGACGGTACTGGCGGCGAACAATACGGGATATCATAATCTGACGTTGCTTATCTAAAATGCGTATCTGCGCGGTTACGGTGCCGCGGGGCCGATAATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTg                                                                                                                 <  1:4024183/144‑1 (MQ=255)
                                gatgaGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTg                                                                                                                 <  2:1301605/150‑1 (MQ=255)
                                  tgaGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGAt                                                                                                               >  2:4760329/1‑150 (MQ=255)
                                  tgaGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGAt                                                                                                               >  2:5174560/1‑150 (MQ=255)
                                  tgaGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGGTGAt                                                                                                               >  1:1337747/1‑150 (MQ=255)
                                        aaCCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCcttct                                                                                                         <  1:1609316/150‑1 (MQ=255)
                                          cccACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGGCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCttt                                                                                                       <  2:3254157/150‑1 (MQ=255)
                                            cACCTGACGGTGCTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTcc                                                                                                     <  1:2244252/150‑1 (MQ=255)
                                            cACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTcc                                                                                                     <  2:1748247/150‑1 (MQ=255)
                                            cACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTcc                                                                                                     <  1:4254080/150‑1 (MQ=255)
                                             aCCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCcg                                                                                                    <  2:835812/150‑1 (MQ=255)
                                                tGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCcggcg                                                                                                 >  2:4865567/1‑150 (MQ=255)
                                                 gACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCcggcgg                                                                                                >  1:602799/1‑150 (MQ=255)
                                                    ggTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACg                                                                                             >  2:3636995/1‑150 (MQ=255)
                                                     gTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGc                                                                                            <  2:3862127/150‑1 (MQ=255)
                                                      tACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCa                                                                                           >  1:1669265/1‑150 (MQ=255)
                                                      tACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCa                                                                                           >  1:2439452/1‑150 (MQ=255)
                                                         tGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATgg                                                                                        <  1:2078913/150‑1 (MQ=255)
                                                         tGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATgg                                                                                        >  1:933102/1‑150 (MQ=255)
                                                         tGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATgg                                                                                        <  1:5262165/150‑1 (MQ=255)
                                                            cggcgAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCg                                                                                     <  2:1060606/150‑1 (MQ=255)
                                                              gctaaCAATACCGGATATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGTGCAGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGAc                                                                                   <  1:3163275/147‑1 (MQ=255)
                                                                 aaCAATACCGGCTATAATAATCTTACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTc                                                                                <  1:4353353/150‑1 (MQ=255)
                                                                    aaTACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCGTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGa                                                                             >  2:1805601/1‑150 (MQ=255)
                                                                        ccGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCa                                                                         <  2:4645511/150‑1 (MQ=255)
                                                                        ccGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACATCGGACGCa                                                                         <  1:3047865/150‑1 (MQ=255)
                                                                            cTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCt                                                                     >  1:470850/1‑150 (MQ=255)
                                                                             tATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCtt                                                                    <  1:748373/150‑1 (MQ=255)
                                                                             tATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCtt                                                                    <  1:2777617/150‑1 (MQ=255)
                                                                             tATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATAATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCtt                                                                    >  2:1476138/1‑150 (MQ=255)
                                                                              aTCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCttt                                                                   >  2:440250/1‑150 (MQ=255)
                                                                              aTCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCttt                                                                   <  2:1226780/150‑1 (MQ=255)
                                                                               tCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCtttt                                                                  <  2:4611436/150‑1 (MQ=255)
                                                                                  gAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCg                                                               <  2:1968838/150‑1 (MQ=255)
                                                                                    aTCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTg                                                             >  2:2642641/1‑150 (MQ=255)
                                                                                    aTCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTGTTGCGTg                                                             >  2:2302831/1‑150 (MQ=255)
                                                                                     tCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTgg                                                            <  2:690632/150‑1 (MQ=255)
                                                                                      cTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGt                                                           <  2:3932621/150‑1 (MQ=255)
                                                                                      cTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGt                                                           >  1:1293714/1‑150 (MQ=255)
                                                                                       tGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGGGGTa                                                          >  1:5027265/1‑150 (MQ=255)
                                                                                       tGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCGTTTGCGTGGTa                                                          >  2:366514/1‑150 (MQ=255)
                                                                                        gACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTaa                                                         >  1:18536/1‑150 (MQ=255)
                                                                                            ttGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAgc                                                     >  1:897347/1‑150 (MQ=255)
                                                                                             tGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAgcg                                                    <  2:1842243/150‑1 (MQ=255)
                                                                                               cTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAgcgcg                                                  >  2:1445652/1‑150 (MQ=255)
                                                                                                 gATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCt                                                >  2:652320/1‑150 (MQ=255)
                                                                                                 gATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGACTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCt                                                >  1:5127710/1‑150 (MQ=255)
                                                                                                    ctcAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGt                                             <  2:3477217/150‑1 (MQ=255)
                                                                                                      cAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAACGCGCTGGTAg                                           >  1:3333597/1‑150 (MQ=255)
                                                                                                       aaaaGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGa                                          >  1:2832580/1‑150 (MQ=255)
                                                                                                       aaaaGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGa                                          >  1:1572304/1‑150 (MQ=255)
                                                                                                          aGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTGTTGCGTGGTAACAGCGCGCTGGTAGATGa                                       >  2:1126363/1‑150 (MQ=255)
                                                                                                           gCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAg                                      <  1:1778525/150‑1 (MQ=255)
                                                                                                           gCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGCCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAg                                      <  2:2615188/150‑1 (MQ=255)
                                                                                                            cGTATCAGCGCGGGTACGGTGCCGCCTGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAgt                                     <  2:3139544/150‑1 (MQ=255)
                                                                                                            cGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAgt                                     >  1:3539130/1‑150 (MQ=255)
                                                                                                             gTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAgtg                                    >  2:2745804/1‑150 (MQ=255)
                                                                                                                tCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAgtgtgt                                 <  1:3978800/150‑1 (MQ=255)
                                                                                                                tCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAgtgtgt                                 >  2:298098/1‑150 (MQ=255)
                                                                                                                 cAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTTACAGCGCGCTGGTAGATGAGTGTGTc                                <  2:2426211/150‑1 (MQ=255)
                                                                                                                 cAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTc                                <  2:4960331/150‑1 (MQ=255)
                                                                                                                  aGCGCGGGTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTcg                               <  1:233461/150‑1 (MQ=255)
                                                                                                                        ggTACGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGtttt                         <  1:4438258/150‑1 (MQ=255)
                                                                                                                            cGGTGCCGCCGGGCCGATCATCGTTCGCGACTGGCTGATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTCACAGCGCGCTGGTCGATGAGTGTGTCGCGTTTTATga                     >  2:1707024/1‑150 (MQ=255)
                                                                                                                                  cgccgGGCCGATCATCGTTCGCGACGGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAaca               <  2:4052089/150‑1 (MQ=255)
                                                                                                                                    ccgGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACt             <  1:4831734/150‑1 (MQ=255)
                                                                                                                                     cgGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACtt            <  1:1027365/150‑1 (MQ=255)
                                                                                                                                     cgGGCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACtt            >  1:4961628/1‑150 (MQ=255)
                                                                                                                                     cgGGCCGATCATCGTTCGCGACTGGC‑TATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTc           <  1:387289/150‑1 (MQ=255)
                                                                                                                                      gggCCGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTc           >  2:3380337/1‑150 (MQ=255)
                                                                                                                                         ccGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCg        <  1:5174560/150‑1 (MQ=255)
                                                                                                                                          cGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCgg       <  1:2632987/150‑1 (MQ=255)
                                                                                                                                          cGATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTGTTATGAAGAACACTTCCCgg       >  2:445209/1‑150 (MQ=255)
                                                                                                                                           gATCATCGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGGGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGa      >  1:808975/1‑150 (MQ=255)
                                                                                                                                            atcatcGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGAt     >  1:1885511/1‑150 (MQ=255)
                                                                                                                                            atcatcGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGAt     >  1:4632568/1‑150 (MQ=255)
                                                                                                                                            atcatcGTTCGCGACAGGCTTATCGAATTAAACGAAGGGTTGATCCTACTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAATAACACTTCCCGGAt     >  1:2688027/1‑150 (MQ=255)
                                                                                                                                             tcatcGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATc    <  2:2155599/150‑1 (MQ=255)
                                                                                                                                              catcGTTCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCg   >  2:2287912/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CAGATTTTAACGTCCAGTGCGACCTGCTGGGTGATGAGTTAACCCACCTGACGGTACTGGCGGCGAACAATACCGGCTATCAGAATCTGACGTTGCTGATCTCAAAAGCGTATCAGCGCGGGTACGGTGCCGCCGGGCCGATCATCGATCGCGACTGGCTTATCGAATTAAACGAAGGGTTGATCCTTCTTTCCGGCGGACGCATGGGCGACGTCGGACGCAGTCTTTTGCGTGGTAACAGCGCGCTGGTAGATGAGTGTGTCGCGTTTTATGAAGAACACTTCCCGGATCGC  >  CP009273/201815‑202107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑