Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,209,162 C→A 100% I9I (ATC→ATA)  yohO → putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,209,1620CA99.7% 1183.9 / ‑4.6 354I9I (ATC→ATA) yohOputative membrane protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (186/167);  total (187/167)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 355 total aligned reads displayed.

CCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATCGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCC  >  CP009273/2209013‑2209308
                                                                                                                                                     |                                                                                                                                                  
ccAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATc                                                                                                                                                    <  2:742016/150‑1 (MQ=255)
  aaCAGCAGCAACTCACCAGTTGTCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATTATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTCACATAAGGAGTGCATTATGCGCATAGATAAAATTGGGGTCATAGc                                                                                                                                                  >  2:4916921/1‑150 (MQ=255)
     agcagcAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCcct                                                                                                                                               >  1:3741398/1‑150 (MQ=255)
        agcaACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCcctgtt                                                                                                                                            >  1:445786/1‑150 (MQ=255)
          caACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCcctgttcc                                                                                                                                          >  1:5030372/1‑150 (MQ=255)
                 ccAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTAt                                                                                                                                   <  1:353495/150‑1 (MQ=255)
                  cAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATg                                                                                                                                  >  2:2739046/1‑150 (MQ=255)
                    gTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGc                                                                                                                                <  2:2398494/150‑1 (MQ=255)
                         cAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTCAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAg                                                                                                                           >  2:4746338/1‑150 (MQ=255)
                         cAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGttcg                                                                                                                           >  2:800153/1‑148 (MQ=255)
                         cAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAg                                                                                                                           <  2:1411912/150‑1 (MQ=255)
                         cAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAg                                                                                                                           <  2:3926744/150‑1 (MQ=255)
                          aaGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAgg                                                                                                                          >  1:2127216/1‑150 (MQ=255)
                          aaGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAgg                                                                                                                          >  2:1282308/1‑150 (MQ=255)
                          aaGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAgg                                                                                                                          <  2:2176755/150‑1 (MQ=255)
                           aGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGc                                                                                                                         <  2:2006070/150‑1 (MQ=255)
                             aaCAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCgg                                                                                                                       >  2:2789667/1‑150 (MQ=255)
                                aaGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAAt                                                                                                                    <  2:3048309/150‑1 (MQ=255)
                                aaGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATAGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAAt                                                                                                                    >  2:4592045/1‑150 (MQ=255)
                                 aGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAAtt                                                                                                                   <  2:5045022/150‑1 (MQ=255)
                                  ggCCATGAGATTGTCCCACTCGGCATTTAATGATTCGTTAACAACTGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATtg                                                                                                                  <  1:2549694/150‑1 (MQ=255)
                                   gCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATtgg                                                                                                                 >  2:4272996/1‑150 (MQ=255)
                                    ccATGATATTTTCCCACTCGGCATATAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATtggc                                                                                                                <  2:3314618/150‑2 (MQ=255)
                                           atTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCa                                                                                                         <  1:3934523/150‑1 (MQ=255)
                                           atTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCa                                                                                                         <  1:4696585/150‑1 (MQ=255)
                                             tttCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATg                                                                                                       <  2:4001403/150‑1 (MQ=255)
                                              ttCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGc                                                                                                      >  1:1032033/1‑150 (MQ=255)
                                               tCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCt                                                                                                     >  1:1195341/1‑150 (MQ=255)
                                                 ccACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGTTCg                                                                                                   <  2:1770445/150‑1 (MQ=255)
                                                 ccACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCg                                                                                                   <  1:4013150/150‑1 (MQ=255)
                                                 ccACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCg                                                                                                   <  1:4846340/150‑1 (MQ=255)
                                                  cACTCGGCATTTAATGATGCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGc                                                                                                  >  2:63721/1‑150 (MQ=255)
                                                      cGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGt                                                                                              <  1:970508/150‑1 (MQ=255)
                                                         cATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTtat                                                                                           >  1:1482422/1‑150 (MQ=255)
                                                         cATTTAATGATTCGTTAACAAATGCGCTGTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTtat                                                                                           <  2:85124/150‑1 (MQ=255)
                                                          aTTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTtata                                                                                          <  1:3265203/150‑1 (MQ=255)
                                                          aTTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTtata                                                                                          <  1:1417884/150‑1 (MQ=255)
                                                          aTTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTtata                                                                                          >  2:1247421/1‑150 (MQ=255)
                                                           tttAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATAc                                                                                         <  1:1689214/150‑1 (MQ=255)
                                                            ttAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATAcc                                                                                        <  2:4504467/150‑1 (MQ=255)
                                                            tgaaTGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATAcc                                                                                        <  2:1915521/148‑1 (MQ=255)
                                                              aaTGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCtt                                                                                      <  1:1996564/150‑1 (MQ=255)
                                                              aaTGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCtt                                                                                      <  2:1185295/150‑1 (MQ=255)
                                                              aaTGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCtt                                                                                      <  2:3610826/150‑1 (MQ=255)
                                                                tGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTa                                                                                    >  1:60213/1‑150 (MQ=255)
                                                                 gATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTAt                                                                                   >  2:2551831/1‑150 (MQ=255)
                                                                  aTTCTTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTAtt                                                                                  >  1:305229/1‑150 (MQ=255)
                                                                  aTTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGACTTGGTGGCGTCATGCTCGCAGGTTATACCTTTAtt                                                                                  >  2:1645904/1‑150 (MQ=255)
                                                                   ttCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTAttt                                                                                 <  1:682359/150‑1 (MQ=255)
                                                                   ttCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTAttt                                                                                 >  1:2336139/1‑150 (MQ=255)
                                                                   ttCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTAttt                                                                                 <  1:4754323/150‑1 (MQ=255)
                                                                     cGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTg                                                                               >  1:1627265/1‑150 (MQ=255)
                                                                      gTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGc                                                                              <  1:4891611/150‑1 (MQ=255)
                                                                        tAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGt                                                                            >  1:3622052/1‑150 (MQ=255)
                                                                         aaCAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTGTGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTg                                                                           <  2:3514439/150‑1 (MQ=255)
                                                                         aaCAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTg                                                                           <  2:1815232/150‑1 (MQ=255)
                                                                         aaCAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTg                                                                           >  2:1541575/1‑150 (MQ=255)
                                                                                  gcTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAg                                                                  <  2:1074522/150‑1 (MQ=255)
                                                                                      tACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACATTTATTTTGCGTGATGGCTAAgcgca                                                              >  1:2090075/1‑149 (MQ=255)
                                                                                        cTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTg                                                            <  2:3193644/150‑1 (MQ=255)
                                                                                        cTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTg                                                            <  1:3572723/150‑1 (MQ=255)
                                                                                        cTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTg                                                            >  1:944239/1‑150 (MQ=255)
                                                                                        cTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTg                                                            >  1:1147971/1‑150 (MQ=255)
                                                                                          gTACACTCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCa                                                          <  1:2639846/150‑1 (MQ=255)
                                                                                           tACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCAc                                                         >  1:3776567/1‑150 (MQ=255)
                                                                                            aCAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATCGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCAcc                                                        >  2:2622984/1‑150 (MQ=255)
                                                                                              aaTCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGAGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAg                                                      >  1:1070225/1‑150 (MQ=255)
                                                                                                   tttCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCttt                                                 <  1:2938681/150‑1 (MQ=255)
                                                                                                   tttCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCttt                                                 >  2:650428/1‑150 (MQ=255)
                                                                                                    ttCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTc                                                >  1:3136445/1‑150 (MQ=255)
                                                                                                     tCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCa                                               <  1:2456534/150‑1 (MQ=255)
                                                                                                       gTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCaaa                                             >  1:3410665/1‑150 (MQ=255)
                                                                                                        ttAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAAc                                            >  2:2318439/1‑150 (MQ=255)
                                                                                                        ttAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAAc                                            >  2:499329/1‑150 (MQ=255)
                                                                                                        ttAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAAc                                            <  2:5202553/150‑1 (MQ=255)
                                                                                                          aaCATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAg                                          <  2:3417065/150‑1 (MQ=255)
                                                                                                          aaCATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAg                                          >  1:4094724/1‑150 (MQ=255)
                                                                                                          aaCATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGAGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAg                                          >  1:4636888/1‑150 (MQ=255)
                                                                                                            cNTAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTCAGCGCCTGCACCAGCCTTTCAAACAGGc                                        >  1:2874284/1‑150 (MQ=255)
                                                                                                            cATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGc                                        <  1:2814007/150‑1 (MQ=255)
                                                                                                             aTAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCg                                       >  1:4218445/1‑150 (MQ=255)
                                                                                                              tAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCgg                                      >  1:4173736/1‑150 (MQ=255)
                                                                                                                aGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGGTAGGCGGAATTGGTGGCGTCATGCTCGCTGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTc                                    <  2:2114317/150‑1 (MQ=255)
                                                                                                                        aTTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCgatga                            >  2:3454247/1‑150 (MQ=255)
                                                                                                                        aTTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCgatga                            >  1:4440341/1‑150 (MQ=255)
                                                                                                                        aTTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCgatga                            >  1:2686401/1‑150 (MQ=255)
                                                                                                                        aTTATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCgatga                            >  1:5188103/1‑150 (MQ=255)
                                                                                                                         ttATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCgatgat                           >  1:2181625/1‑150 (MQ=255)
                                                                                                                         ttATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCgatgat                           <  1:1804729/150‑1 (MQ=255)
                                                                                                                         ttATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCgatgat                           <  2:4834121/150‑1 (MQ=255)
                                                                                                                         ttATGCGCATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCgatgat                           <  2:3739653/150‑1 (MQ=255)
                                                                                                                             gcgcATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATcgcc                       <  2:4448777/150‑1 (MQ=255)
                                                                                                                              cgcATAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATcgccg                      <  2:2656035/150‑1 (MQ=255)
                                                                                                                                  tAGCTAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAg                  >  2:5315268/1‑150 (MQ=255)
                                                                                                                                      tAAAATTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGcc              >  1:3373566/1‑150 (MQ=255)
                                                                                                                                          aTTGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAATGccacca          <  1:2589055/150‑1 (MQ=255)
                                                                                                                                           ttGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAg         <  1:823033/150‑1 (MQ=255)
                                                                                                                                            tGGGGTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGt        <  1:2218479/150‑1 (MQ=255)
                                                                                                                                                gTCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTCACg    >  2:3181602/1‑150 (MQ=255)
                                                                                                                                                 tCATAGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGc   <  1:4272996/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CCAACAGCAGCAACTCACCAGTTGGCAAGAACAAGGCCATGATATTTTCCCACTCGGCATTTAATGATTCGTTAACAAATGCGCTTTACTGTACAATCCTTTCGTTAACATAAGGAGTGCATTATGCGCATAGCTAAAATTGGGGTCATCGCCCTGTTCCTGTTTATGGCGTTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCC  >  CP009273/2209013‑2209308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑