Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,241,222 T→G 100% I264L (ATT→CTT)  lysP ← lysine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,241,2220TG99.0% 1125.0 / ‑2.6 283I264L (ATT→CTT) lysPlysine transporter
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base G (150/130);  total (150/133)
Fisher's exact test for biased strand distribution p-value = 1.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 288 total aligned reads displayed.

ACCCGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGAAGC  >  CP009273/2241076‑2241368
                                                                                                                                                  |                                                                                                                                                  
aCCCGCTGACAGCAGCGCCGTCAGAATAACTGCGTTCATCTCCGCCGGCGCAGAGAGCAGACCCGCGTGCTGGATGACCTGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTTCGGaagaat                                                                                                                                                 <  2:3289288/150‑1 (MQ=255)
   cGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAg                                                                                                                                              <  2:2790599/150‑1 (MQ=255)
   cGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAg                                                                                                                                              <  1:597343/150‑1 (MQ=255)
     cTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGc                                                                                                                                            >  2:286680/1‑150 (MQ=255)
         cAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGAt                                                                                                                                        <  2:81534/150‑1 (MQ=255)
          aGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATa                                                                                                                                       >  2:2252099/1‑150 (MQ=255)
          aGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATa                                                                                                                                       >  2:4696247/1‑150 (MQ=255)
          aGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATa                                                                                                                                       <  1:2212437/150‑1 (MQ=255)
              ccgccgTCAGAAGAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCa                                                                                                                                   <  1:1005461/150‑1 (MQ=255)
                  cgTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGAt                                                                                                                               <  1:2935552/150‑1 (MQ=255)
                   gTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATc                                                                                                                              <  1:4752696/150‑1 (MQ=255)
                     cAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATcgc                                                                                                                            >  2:4138884/1‑150 (MQ=255)
                       gAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGa                                                                                                                          >  1:2426913/1‑150 (MQ=255)
                          tAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAaca                                                                                                                       >  2:314594/1‑150 (MQ=255)
                                cGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGa                                                                                                                 >  2:2193737/1‑150 (MQ=255)
                                cGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGa                                                                                                                 >  1:371832/1‑150 (MQ=255)
                                 gTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGaa                                                                                                                >  2:3460398/1‑150 (MQ=255)
                                 gTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGaa                                                                                                                >  1:58297/1‑150 (MQ=255)
                                  ttCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGaac                                                                                                               <  1:635396/150‑1 (MQ=255)
                                   tcatcaCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGaaca                                                                                                              <  2:3558780/150‑1 (MQ=255)
                                   tcatcaCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGaaca                                                                                                              <  2:4013084/150‑1 (MQ=255)
                                     atcaCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGaacaac                                                                                                            <  1:3141308/150‑1 (MQ=255)
                                     atcaCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGaacaac                                                                                                            >  2:4769031/1‑150 (MQ=255)
                                     atcaCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGaacaac                                                                                                            >  1:3814413/1‑150 (MQ=255)
                                      tcaCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAacaaca                                                                                                           <  2:3026431/150‑1 (MQ=255)
                                      tcaCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAacaaca                                                                                                           >  2:5250122/1‑150 (MQ=255)
                                       caCCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGACGAATCAGGCTGATAATCAGGATCGCGAACACCTAGAACAACAg                                                                                                          >  2:3759924/1‑150 (MQ=255)
                                          cgccgccgcAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGAt                                                                                                       <  1:2610049/150‑1 (MQ=255)
                                                cgcAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCa                                                                                                 <  1:271411/150‑1 (MQ=255)
                                                 gcAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAg                                                                                                <  1:1506346/150‑1 (MQ=255)
                                                   agagagCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGaa                                                                                              >  1:4425758/1‑150 (MQ=255)
                                                   agagagCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGaa                                                                                              >  2:2385047/1‑150 (MQ=255)
                                                    gagagCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAac                                                                                             <  2:3588642/150‑1 (MQ=255)
                                                     agagCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAaca                                                                                            >  2:1070887/1‑150 (MQ=255)
                                                     agagCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAaca                                                                                            <  1:5194389/150‑1 (MQ=255)
                                                      gagCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAacac                                                                                           <  2:44169/150‑1 (MQ=255)
                                                         cAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTg                                                                                        >  2:2638354/1‑150 (MQ=255)
                                                           gACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGAc                                                                                      <  1:4865597/150‑1 (MQ=255)
                                                           gACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGAc                                                                                      <  1:822019/150‑1 (MQ=255)
                                                               cgcgTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTAc                                                                                  >  2:618591/1‑150 (MQ=255)
                                                               cgcgTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTAc                                                                                  <  2:1973481/150‑1 (MQ=255)
                                                                  gTGCTGGAACACCAGGGTGACCGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATCATCAGGATCGCGAACACATCGAACAACAGGATTCGCCAGAACACCTGACGTACcgc                                                                               >  2:1203332/1‑150 (MQ=255)
                                                                  gTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACcgc                                                                               >  2:1330886/1‑150 (MQ=255)
                                                                  gTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACcgc                                                                               >  1:849189/1‑150 (MQ=255)
                                                                      tGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGt                                                                           >  2:4437479/1‑150 (MQ=255)
                                                                       ggAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTATGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTg                                                                          >  1:2027312/1‑150 (MQ=255)
                                                                       ggAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTg                                                                          <  1:1321405/150‑1 (MQ=255)
                                                                            acCAGGGTGAACGGACTTACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTTATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATg                                                                     <  2:5163144/150‑1 (MQ=255)
                                                                            acCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATg                                                                     <  2:1626591/150‑1 (MQ=255)
                                                                             ccAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGt                                                                    <  1:2000181/150‑1 (MQ=255)
                                                                              cAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGtt                                                                   >  1:928140/1‑150 (MQ=255)
                                                                               aGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGttt                                                                  <  2:621037/150‑1 (MQ=255)
                                                                               aGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGttt                                                                  <  2:3852792/150‑1 (MQ=255)
                                                                                gggTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGACTGtttt                                                                 >  1:1833787/1‑150 (MQ=255)
                                                                                gggTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGtttt                                                                 >  2:44503/1‑150 (MQ=255)
                                                                                gggTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGtttt                                                                 <  2:2129652/150‑1 (MQ=255)
                                                                                 ggTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGttttt                                                                >  2:1481190/1‑150 (MQ=255)
                                                                                  gTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTc                                                               >  1:2304394/1‑150 (MQ=255)
                                                                                     aaCGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGTAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGcc                                                            >  1:1961610/1‑150 (MQ=255)
                                                                                     aaCGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGcc                                                            >  2:2278316/1‑150 (MQ=255)
                                                                                     aaCGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGcc                                                            <  1:2524141/150‑1 (MQ=255)
                                                                                          aCTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATc                                                       >  2:4981165/1‑150 (MQ=255)
                                                                                          aCTAACGCTGANGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATc                                                       >  1:2759560/1‑150 (MQ=255)
                                                                                             aaCGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTc                                                    <  1:5043544/150‑1 (MQ=255)
                                                                                             aaCGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCCGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTc                                                    <  2:1936434/150‑1 (MQ=255)
                                                                                             aaCGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTAGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTc                                                    <  1:1611136/150‑1 (MQ=255)
                                                                                               cGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCgg                                                  >  2:2705229/1‑150 (MQ=255)
                                                                                                  tGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACt                                               <  2:3386441/150‑1 (MQ=255)
                                                                                                   gATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTc                                              <  1:3409821/150‑1 (MQ=255)
                                                                                                    aTGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCg                                             >  1:1255747/1‑150 (MQ=255)
                                                                                                    aTGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCg                                             >  1:3900311/1‑150 (MQ=255)
                                                                                                     tGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGc                                            >  1:4362220/1‑150 (MQ=255)
                                                                                                       tCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCt                                          >  2:3108157/1‑150 (MQ=255)
                                                                                                        cTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTg                                         >  1:4488100/1‑150 (MQ=255)
                                                                                                            aaCATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTgcagc                                     >  1:3498792/1‑150 (MQ=255)
                                                                                                              cATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCaa                                   >  1:2793187/1‑150 (MQ=255)
                                                                                                                 cGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGACTGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATAc                                >  2:977842/1‑150 (MQ=255)
                                                                                                                 cGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATAc                                >  2:2850854/1‑150 (MQ=255)
                                                                                                                 cGTTACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATAc                                >  2:1018086/1‑150 (MQ=255)
                                                                                                                   ttACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCg                              <  2:2576027/150‑1 (MQ=255)
                                                                                                                    tACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGa                             >  2:1749443/1‑150 (MQ=255)
                                                                                                                    tACGCAGCAGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGa                             >  2:1561138/1‑150 (MQ=255)
                                                                                                                       gcagcaGGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCa                          <  2:667736/150‑1 (MQ=255)
                                                                                                                        cagcagGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAg                         <  1:4175570/150‑1 (MQ=255)
                                                                                                                        cagcagGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAg                         >  1:4992680/1‑150 (MQ=255)
                                                                                                                         agcagGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGc                        <  2:4645627/150‑1 (MQ=255)
                                                                                                                         agcagGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGc                        >  1:1784937/1‑150 (MQ=255)
                                                                                                                           cagGCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTc                      <  2:4235877/150‑1 (MQ=255)
                                                                                                                             ggCTCGGATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCgg                    >  2:4861104/1‑150 (MQ=255)
                                                                                                                                  ggATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTccc               <  2:2970395/150‑1 (MQ=255)
                                                                                                                                   gATCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCt              <  1:4328886/150‑1 (MQ=255)
                                                                                                                                    aTCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTg             <  1:5292112/150‑1 (MQ=255)
                                                                                                                                    aTCGGTGTACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTg             <  2:373749/150‑1 (MQ=255)
                                                                                                                                        gtgtACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGaaa         >  1:4279457/1‑150 (MQ=255)
                                                                                                                                        gtgtACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGaaa         >  1:4020797/1‑150 (MQ=255)
                                                                                                                                         tgtACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAag        >  2:5127271/1‑150 (MQ=255)
                                                                                                                                         tgtACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAag        >  1:3300348/1‑150 (MQ=255)
                                                                                                                                          gtACGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAaga       >  2:5017597/1‑150 (MQ=255)
                                                                                                                                             cGGAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGaa    <  1:3968614/150‑1 (MQ=255)
                                                                                                                                              ggAAGAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGAAg   >  1:3628587/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACCCGCTGACAGCACCGCCGTCAGAATAACTGCGTTCATCACCGCCGCCGCAGAGAGCAGACCCGCGTGCTGGAACACCAGGGTGAACGGACTAACGCTGATGTCTTTAACATCGTTACGCAGCAGGCTCGGATCGGTGTACGGAATAATCAGGCTGATAATCAGGATCGCGAACACATAGAACAACAGGATTCGCCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGAAGC  >  CP009273/2241076‑2241368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑