BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,247,093	A→T	6.1%	C178S (TGT→AGT)	nupX ←	nucleoside permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,247,093	0	A	T	6.1%	1203.9 / 49.5	391	C178S (TGT→AGT)	nupX	nucleoside permease
Reads supporting (aligned to +/- strand): ref base A (161/206); new base T (11/13); total (172/219)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

Only 100 of 411 total aligned reads displayed.

CGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCTTTCTGGAAGATACCGCCGA  >  CP009273/2246944‑2247242

 gAAGATTCCGTTGCCTGTCTTAACAGGCGGTCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGTCGTAACCAATCTTTGTCGAACCAGCAATCGAGTCCATTCCACTaaaa                                                                                                                                                      <  2:2878338/150‑1 (MQ=38)

 gAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACaa                                                                                                                                                      >  1:1326094/1‑150 (MQ=255)

 gAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACaa                                                                                                                                                      >  2:2421382/1‑150 (MQ=255)

  aaGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACaaa                                                                                                                                                     >  1:1509730/1‑150 (MQ=255)

     aTTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCg                                                                                                                                                  >  1:4701603/1‑150 (MQ=255)

     aTTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCg                                                                                                                                                  >  2:4682022/1‑150 (MQ=255)

       tCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCt                                                                                                                                                <  2:1719704/150‑1 (MQ=255)

        ccGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTg                                                                                                                                               <  2:2529832/150‑1 (MQ=255)

         cGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGt                                                                                                                                              <  1:2979386/150‑1 (MQ=255)

           ttGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTaa                                                                                                                                            <  2:289211/150‑1 (MQ=255)

             gCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTaaag                                                                                                                                          <  2:2375033/150‑2 (MQ=255)

             gCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAaat                                                                                                                                          <  1:1538480/150‑1 (MQ=255)

                gggCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAaataat                                                                                                                                       <  1:4582125/150‑1 (MQ=255)

                 ggCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAAtt                                                                                                                                      >  2:4498626/1‑150 (MQ=255)

                  gCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTc                                                                                                                                     <  1:2110522/150‑1 (MQ=255)

                   cTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCa                                                                                                                                    <  2:4223724/150‑1 (MQ=255)

                   cTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCa                                                                                                                                    >  2:4000512/1‑150 (MQ=255)

                    ttAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCAt                                                                                                                                   >  1:540144/1‑150 (MQ=255)

                     tAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCAtt                                                                                                                                  <  1:1951605/150‑1 (MQ=255)

                     tAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCAtt                                                                                                                                  <  1:2638182/150‑1 (MQ=255)

                         aGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTACTGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGGAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGa                                                                                                                              <  1:1697227/150‑1 (MQ=255)

                           gCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGAtt                                                                                                                            <  1:518548/150‑1 (MQ=255)

                            cGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTc                                                                                                                           <  2:3878804/150‑1 (MQ=255)

                              ggCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAg                                                                                                                         <  2:1812435/150‑1 (MQ=255)

                              ggCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAg                                                                                                                         >  1:475887/1‑150 (MQ=255)

                                 aaaCAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGAcg                                                                                                                      >  2:2363613/1‑150 (MQ=255)

                                     aaGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGAcgatcg                                                                                                                  <  1:2376242/150‑1 (MQ=255)

                                     aaGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGAcgatcg                                                                                                                  >  1:2356248/1‑150 (MQ=255)

                                        aTCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATa                                                                                                               >  2:4750028/1‑150 (MQ=255)

                                        aTCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATa                                                                                                               >  2:489286/1‑150 (MQ=255)

                                          cccGCCAGGGATCGCCATTAATGATGCCGACAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATAGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATaaa                                                                                                             <  1:3072708/150‑1 (MQ=255)

                                             gCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAggg                                                                                                          <  1:4361654/150‑1 (MQ=255)

                                              ccAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGt                                                                                                         >  2:1594217/1‑150 (MQ=255)

                                               cAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGtt                                                                                                        <  1:4798609/150‑1 (MQ=255)

                                                aGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGttt                                                                                                       <  1:4866493/150‑1 (MQ=255)

                                                aGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGttt                                                                                                       <  2:1463946/150‑1 (MQ=255)

                                                aGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGttt                                                                                                       <  1:292700/150‑1 (MQ=255)

                                                  ggATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGa                                                                                                     >  1:4275051/1‑150 (MQ=255)

                                                  ggATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAAGAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGa                                                                                                     <  1:808711/150‑1 (MQ=255)

                                                          aTTCATGCTGCCGCCAGCAGATATTCCTCAGGCACGCCCAGTGCGGCGTAACCTATCCTTGTCGAACCAGCAATCGAGGCCTTGCCACTACAAATCGCTGTAAAGAATTCAGTGCGATTCAGACGATCGATTAAGGGTTTGACGATTGct                                                                                             <  2:4582330/150‑2 (MQ=255)

                                                          aTTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGcc                                                                                             >  1:3681478/1‑150 (MQ=255)

                                                          aTTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGcc                                                                                             >  1:1075233/1‑150 (MQ=255)

                                                          aTTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAAGAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGcc                                                                                             <  2:1926673/150‑1 (MQ=255)

                                                              atgatgCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGaa                                                                                         >  1:3572048/1‑150 (MQ=255)

                                                               tgatgCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAAt                                                                                        >  1:1010777/1‑150 (MQ=255)

                                                               tgatgCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCAGTGTCGAACCAGCAATCGAGGCCATGCCACTACTAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAAt                                                                                        <  2:4127743/150‑1 (MQ=255)

                                                                      gccAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGttt                                                                                 >  2:3156224/1‑150 (MQ=255)

                                                                       ccAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGtttt                                                                                <  2:2523494/150‑1 (MQ=255)

                                                                       ccAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGtttt                                                                                <  2:2176931/150‑1 (MQ=255)

                                                                          gcagATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCCTGCCACTACAAAGCGCTGTAAATAAATCATTGCGATTCAGACGATCGATACAGGGTTTGACGAGTGCCGGAAGTTCGTTTTGcc                                                                             >  2:868795/1‑150 (MQ=255)

                                                                          gcagATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACTAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGcc                                                                             >  1:3227704/1‑150 (MQ=255)

                                                                           cagATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGccc                                                                            >  2:3524656/1‑150 (MQ=255)

                                                                             gATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGa                                                                          >  1:3498658/1‑150 (MQ=255)

                                                                                atTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGa                                                                       <  1:1252757/150‑1 (MQ=255)

                                                                                   ccACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGaaaa                                                                    <  1:380752/150‑1 (MQ=255)

                                                                                    cacaGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAAt                                                                   <  1:2690755/150‑1 (MQ=255)

                                                                                     acaGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAAtg                                                                  >  2:3048531/1‑150 (MQ=255)

                                                                                      caGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGTCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAAtgg                                                                 >  2:3661569/1‑150 (MQ=255)

                                                                                          cACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAACTCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATggtggt                                                             <  1:5295030/150‑1 (MQ=255)

                                                                                           aCGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACTAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGgtggtg                                                            >  2:4313242/1‑150 (MQ=255)

                                                                                           aCGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGgtggtg                                                            <  2:3693725/150‑1 (MQ=255)

                                                                                             gCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGAc                                                          <  1:1126135/150‑1 (MQ=255)

                                                                                              cccAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGAcc                                                         >  1:2445185/1‑150 (MQ=255)

                                                                                                       gcgTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAAt                                                <  2:3603804/150‑1 (MQ=255)

                                                                                                        cgTAACCAATCATTGTCGAACCAGCAATCGTGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATg                                               <  1:963964/150‑1 (MQ=255)

                                                                                                        cgTAACCAATCATTGTCGAACCAGCAATCGAGTCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATg                                               <  2:2814438/150‑1 (MQ=255)

                                                                                                         gTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGa                                              >  2:4280724/1‑150 (MQ=255)

                                                                                                            aCCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTc                                           <  1:2808572/150‑1 (MQ=255)

                                                                                                             ccAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCg                                          >  1:723970/1‑150 (MQ=255)

                                                                                                              cagtCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGACTGACTCGa                                         >  1:4400395/4‑150 (MQ=255)

                                                                                                               aaTCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGAt                                        >  1:663975/1‑150 (MQ=255)

                                                                                                                aTCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATc                                       <  2:1405293/150‑1 (MQ=255)

                                                                                                                 tCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCt                                      <  2:1509730/150‑1 (MQ=255)

                                                                                                                   aTTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTg                                    <  1:1806833/150‑1 (MQ=255)

                                                                                                                            ccAGCAATCGAGGCCATGCCACTACTAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATAttt                           <  2:5224340/150‑1 (MQ=255)

                                                                                                                            ccAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATAttt                           >  2:5314646/1‑150 (MQ=255)

                                                                                                                            ccAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATAttt                           <  2:1326094/150‑1 (MQ=255)

                                                                                                                               gCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAAt                        <  2:4094157/150‑1 (MQ=255)

                                                                                                                                    cGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCttt                   <  2:696323/150‑1 (MQ=255)

                                                                                                                                    cGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCttt                   <  2:2660601/150‑1 (MQ=255)

                                                                                                                                    cGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCttt                   <  2:1980144/150‑1 (MQ=255)

                                                                                                                                    cGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCttt                   <  2:593493/150‑1 (MQ=255)

                                                                                                                                     gAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCTTTc                  >  1:4164072/1‑150 (MQ=255)

                                                                                                                                     gAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCTTTc                  <  2:2256712/150‑1 (MQ=255)

                                                                                                                                      aGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGTTTTCt                 <  1:1485206/150‑1 (MQ=255)

                                                                                                                                          cATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCTTTCTGGaa             >  2:122139/1‑150 (MQ=255)

                                                                                                                                          cATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCTTTCTGGaa             >  2:5012003/1‑150 (MQ=255)

                                                                                                                                          cATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGGGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCTTTCTGGaa             >  2:1858743/1‑150 (MQ=255)

                                                                                                                                           aTGCCACTTCTAATCTCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCTTTCTGGAAg            <  1:4036165/150‑1 (MQ=255)

                                                                                                                                            tGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCTTTCTGGAAGa           <  1:5080386/150‑1 (MQ=255)

                                                                                                                                            tGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCTTTCTGGAAGa           >  1:31305/1‑150 (MQ=255)

                                                                                                                                              ccACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCTTTCTGGAAGATa         >  1:222218/1‑150 (MQ=255)

                                                                                                                                              ccACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCTTTCTGGAAGATa         >  1:4987077/1‑150 (MQ=255)

                                                                                                                                              ccACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCTTTCTGGAAGATa         >  2:2514941/1‑150 (MQ=255)

                                                                                                                                              ccACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCTTTCTGGAAGATa         >  2:2731280/1‑150 (MQ=255)

                                                                                                                                               cACTACTAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCTTTCTGGAAGATAc        >  2:324304/1‑150 (MQ=255)

                                                                                                                                                actactAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCTTTCTGGAAGATAcc       >  2:3295/1‑150 (MQ=255)

                                                                                                                                                aCTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCTTTCTGGAAGATAcc       <  1:4498626/150‑1 (MQ=255)

                                                                                                                                                    cAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCTTTCTGGAAGATAccgccg   >  1:333656/1‑150 (MQ=255)

                                                                                                                                                     aaaTCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCTTTCTGGAAGATACCGCCga  >  1:4068991/1‑150 (MQ=255)

CGAAGATTCCGTTGCCGGGCTTAACAGGCGGGCAAACAAGATCCCGCCAGGGATCGCCATTAATGATGCCGCCAGCAGATATTCCACAGGCACGCCCAGTGCGGCGTAACCAATCATTGTCGAACCAGCAATCGAGGCCATGCCACTACAAATCGCTGTAAATAATTCATTGCGATTCAGACGATCGATAAAGGGTTTGACGATTGCCGGAATTTCGTTTTGCCCGAGGAAAATGGTGGTGACCGCGACGAATGACTCGATCTTGCTGATATTTAATGCTTTCTGGAAGATACCGCCGA  >  CP009273/2246944‑2247242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`