Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,248,657 C→A 100% K3N (AAG→AAT)  rihB ← ribonucleoside hydrolase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,248,6570CA99.3% 1031.8 / ‑2.4 324K3N (AAG→AAT) rihBribonucleoside hydrolase 2
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base A (168/152);  total (171/153)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 325 total aligned reads displayed.

ATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAA  >  CP009273/2248510‑2248803
                                                                                                                                                   |                                                                                                                                                  
aTTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTAtt                                                                                                                                                  <  2:1946642/150‑1 (MQ=255)
  tCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTAtttt                                                                                                                                                >  2:5280761/1‑150 (MQ=255)
   cAGGCCATTAATTAATGTTTTATCAAGCGACTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAAACCAGAATAATTTTTCTAtttta                                                                                                                                               >  2:1327043/1‑149 (MQ=255)
    aGGCCATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTcc                                                                                                                                              <  1:5058733/150‑1 (MQ=255)
      gCCATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCAt                                                                                                                                            <  1:3296149/150‑1 (MQ=255)
       ccATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTAttttaaatt                                                                                                                                           >  2:111749/1‑145 (MQ=255)
        cATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCTGATCACAATCAAGAATAATTTTTCTATTTTCCATTg                                                                                                                                          >  2:654301/1‑150 (MQ=255)
         attaattaATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGt                                                                                                                                         >  2:178782/1‑150 (MQ=255)
          ttaattaaTGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGtt                                                                                                                                        <  1:4055824/150‑1 (MQ=255)
           taaataatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCAGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGttt                                                                                                                                       <  2:900622/150‑1 (MQ=255)
            aattactGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTa                                                                                                                                      <  1:3245391/150‑1 (MQ=255)
            aattaatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTa                                                                                                                                      <  1:5136160/150‑1 (MQ=255)
            aattaatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTa                                                                                                                                      <  1:4621514/150‑1 (MQ=255)
            aattaatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTa                                                                                                                                      >  2:1982316/1‑150 (MQ=255)
            aattaatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTa                                                                                                                                      >  1:324305/1‑150 (MQ=255)
            aattaatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTa                                                                                                                                      >  2:581654/1‑150 (MQ=255)
            aattaatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTa                                                                                                                                      <  1:1450580/150‑1 (MQ=255)
             attaatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTAt                                                                                                                                     <  2:2195295/150‑1 (MQ=255)
               taatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTAttt                                                                                                                                   <  1:356642/150‑1 (MQ=255)
               taatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTAttt                                                                                                                                   <  1:91596/150‑1 (MQ=255)
                aatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTc                                                                                                                                  >  2:420540/1‑150 (MQ=255)
                aatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAAACTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTc                                                                                                                                  <  1:1012145/150‑1 (MQ=255)
                   gTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCctc                                                                                                                               <  1:991988/150‑1 (MQ=255)
                   gTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCctc                                                                                                                               <  1:4269608/150‑1 (MQ=255)
                    ttttATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCctct                                                                                                                              <  1:4101335/150‑1 (MQ=255)
                       tATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGtt                                                                                                                           >  1:5280790/1‑150 (MQ=255)
                               gTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCCTTGTTTATTTCCTCTGTTTCCAGTTg                                                                                                                   >  2:3418477/1‑150 (MQ=255)
                                 cTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCg                                                                                                                 >  1:1670425/1‑150 (MQ=255)
                                 cTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCg                                                                                                                 >  2:4928868/1‑150 (MQ=255)
                                   gATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGtt                                                                                                               >  2:695775/1‑150 (MQ=255)
                                   gATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGtt                                                                                                               <  2:1148872/150‑1 (MQ=255)
                                    aTTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTa                                                                                                              >  2:2855421/1‑150 (MQ=255)
                                     ttACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTAt                                                                                                             <  1:1790264/150‑1 (MQ=255)
                                      tACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTAtt                                                                                                            <  1:1147386/150‑1 (MQ=255)
                                           gCTACAATAGTGATGCCTAAGAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTc                                                                                                       <  2:5314756/150‑1 (MQ=255)
                                            cTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCt                                                                                                      >  2:4877658/1‑150 (MQ=255)
                                            cTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAAGAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCt                                                                                                      <  1:3561958/150‑1 (MQ=255)
                                              aCAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTAc                                                                                                    <  1:4406573/150‑1 (MQ=255)
                                                aaTAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAg                                                                                                  >  2:4252206/1‑150 (MQ=255)
                                                aaTAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAg                                                                                                  >  1:756402/1‑150 (MQ=255)
                                                   aGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCaa                                                                                               >  2:4109223/1‑150 (MQ=255)
                                                     tGATGCCTAATATATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAg                                                                                             <  1:2989962/150‑1 (MQ=255)
                                                       aTGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGaa                                                                                           >  1:2961479/1‑150 (MQ=255)
                                                       aTGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGaa                                                                                           >  2:1051027/1‑150 (MQ=255)
                                                        tGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGaaa                                                                                          <  2:2373381/150‑1 (MQ=255)
                                                        tGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGaaa                                                                                          >  1:5025095/1‑150 (MQ=255)
                                                          ccTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAAt                                                                                        <  2:4976818/150‑1 (MQ=255)
                                                             aataaATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCg                                                                                     >  1:4367198/1‑150 (MQ=255)
                                                              ataaATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGa                                                                                    >  1:1209322/1‑150 (MQ=255)
                                                               taaATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGAc                                                                                   <  1:4974099/150‑1 (MQ=255)
                                                                    cTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTCTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGc                                                                              >  2:3302856/1‑150 (MQ=255)
                                                                     tagtGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGcc                                                                             <  1:3530602/147‑1 (MQ=255)
                                                                          ccGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCgatga                                                                        >  2:5158745/1‑150 (MQ=255)
                                                                            ggttGTTTCGCCGCCATCTTTATAGAAATAGCATCATCAGGACCCGTATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGAtt                                                                      <  2:277115/147‑1 (MQ=255)
                                                                            ggATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGAtt                                                                      <  1:85926/150‑1 (MQ=255)
                                                                            ggATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGAtt                                                                      >  2:3990364/1‑150 (MQ=255)
                                                                                 tttCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACAGGGCCGATGATTGAATc                                                                 >  2:3533015/1‑150 (MQ=255)
                                                                                  tacgccgcCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCt                                                                <  2:3127866/148‑1 (MQ=255)
                                                                                   tCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGCGCACAATCCAGAATAATTGTTCTATTTTCCATGGTTTCTTTCCTCTGTTTCCCGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCtt                                                               <  2:4523540/150‑1 (MQ=255)
                                                                                   tCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCtt                                                               <  2:3074431/150‑1 (MQ=255)
                                                                                      ccgccATAATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTaac                                                            <  2:4021698/150‑1 (MQ=255)
                                                                                        gccATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTaacaa                                                          >  1:4685526/1‑150 (MQ=255)
                                                                                         ccATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGACAATTCGACCGGGCCGATGATTGAATCTTaacaac                                                         >  2:1535242/1‑150 (MQ=255)
                                                                                         ccATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTaacaac                                                         >  2:1274444/1‑150 (MQ=255)
                                                                                           atcatTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTTAATCTTAACAACAg                                                       >  1:3936977/1‑150 (MQ=255)
                                                                                           atcatTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTATATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAg                                                       <  1:2838640/150‑1 (MQ=255)
                                                                                           atcatTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTCCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAg                                                       <  1:4210684/150‑1 (MQ=255)
                                                                                             catTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGcg                                                     >  2:3635874/1‑150 (MQ=255)
                                                                                             catTATAGCAATAGCATCATCATGAACCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTACTCTGTTTCCAGTTGCGTTATTTTTTCTACATCAAAAAAAATTAGACCTGGCCGATGATTGATTCATAAAAAAAGcc                                                     >  1:4981077/1‑149 (MQ=255)
                                                                                              atTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGcgt                                                    >  1:2452939/1‑150 (MQ=255)
                                                                                                tataGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGcgtac                                                  <  1:259426/150‑1 (MQ=255)
                                                                                                   agcaatagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAAATCGACCGGGCCGATGATTGAATCTTAACAACAGcgtacgta                                               >  1:2408969/1‑150 (MQ=255)
                                                                                                         agcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTaa                                         >  2:3290127/1‑150 (MQ=255)
                                                                                                           catcatcatGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAat                                       >  1:549345/1‑150 (MQ=255)
                                                                                                              catcatTACCCGGATCACAATCCATAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATg                                    <  2:1866716/150‑1 (MQ=255)
                                                                                                              catcatGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCAATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATg                                    <  1:2332155/150‑1 (MQ=255)
                                                                                                              catcatGACCCGGATCACAATCCAGAATAATGTTTCTATTTTCCAGTGTTTATTTCCTCTGTTTCCAGTTGCGTTATATTTTCTACAGCAAAGAAAATACGACCGGGCCCATGATTGAAACTTAACAACCGCCTACGGATGCGAAATATg                                    >  1:4520940/1‑150 (MQ=255)
                                                                                                                tcatGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTTCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATgag                                  <  1:3847260/150‑1 (MQ=255)
                                                                                                                tcatGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATgag                                  <  1:1765914/150‑1 (MQ=255)
                                                                                                                 catGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATgaga                                 >  1:3441259/1‑150 (MQ=255)
                                                                                                                 catGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATgaga                                 >  2:5234458/1‑150 (MQ=255)
                                                                                                                  atGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGaa                                <  2:4840662/150‑1 (MQ=255)
                                                                                                                   tGACCCGGATCACAATCCATAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGaaa                               >  2:2148440/1‑150 (MQ=255)
                                                                                                                        cGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCa                          <  1:1095969/150‑1 (MQ=255)
                                                                                                                           aTCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAg                       >  1:1978370/1‑150 (MQ=255)
                                                                                                                            tCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAATAAAATTCGACCGGGCCGATGATTGTATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGc                      >  1:1373802/1‑150 (MQ=255)
                                                                                                                            tCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCTACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGc                      >  2:3825330/1‑150 (MQ=255)
                                                                                                                              acaATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCgg                    >  2:2068804/1‑150 (MQ=255)
                                                                                                                               caATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGa                   >  1:3376555/1‑150 (MQ=255)
                                                                                                                                aaTCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGAt                  <  2:4711417/150‑1 (MQ=255)
                                                                                                                                     aGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACa             >  1:3080126/1‑150 (MQ=255)
                                                                                                                                     aGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAAACTTAACAACAGCGTACGAATGCTAAAAATGAGAAAACACAAACCGGATAAACa             >  1:4885657/1‑150 (MQ=255)
                                                                                                                                      gAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACAt            >  2:2936384/1‑150 (MQ=255)
                                                                                                                                       aataatTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATATCATAGCGGATAAACAta           >  1:205764/1‑149 (MQ=255)
                                                                                                                                         taatTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATTAGAAATCTCATAGCGGATAAACATCGt         >  1:647863/1‑150 (MQ=255)
                                                                                                                                         taatTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGt         <  2:4435462/150‑1 (MQ=255)
                                                                                                                                             ttttCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTgaaa     <  1:4877658/150‑1 (MQ=255)
                                                                                                                                               ttCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTgaaaga   >  1:4747465/1‑150 (MQ=255)
                                                                                                                                               ttCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTgaaaga   >  1:1566666/1‑150 (MQ=255)
                                                                                                                                                tctaTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTgaaagaa  >  2:2567230/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAA  >  CP009273/2248510‑2248803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑