Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,287,829 C→A 100% S185S (TCG→TCT)  ccmF ← heme lyase, CcmF subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,287,8290CA99.2% 876.0 / ‑2.2 272S185S (TCG→TCT) ccmFheme lyase, CcmF subunit
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base A (118/151);  total (118/154)
Fisher's exact test for biased strand distribution p-value = 2.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 277 total aligned reads displayed.

GAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGAACAGCAA  >  CP009273/2287682‑2287975
                                                                                                                                                   |                                                                                                                                                  
gAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAga                                                                                                                                                  >  2:538821/1‑150 (MQ=255)
gAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAga                                                                                                                                                  <  2:3638223/150‑1 (MQ=255)
 aGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACagag                                                                                                                                                 >  2:4689831/1‑150 (MQ=255)
 aGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACagag                                                                                                                                                 <  2:1301367/150‑1 (MQ=255)
     cgaaaTAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAAc                                                                                                                                             <  2:1573141/147‑1 (MQ=255)
      gTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAAcc                                                                                                                                            <  2:5052177/150‑1 (MQ=255)
        aaTAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAGTTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCa                                                                                                                                          >  1:4222876/1‑150 (MQ=255)
        aaTAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCa                                                                                                                                          >  2:1805564/1‑150 (MQ=255)
         aTAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCAc                                                                                                                                         <  2:3595765/150‑1 (MQ=255)
           aGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGt                                                                                                                                       <  2:4817647/150‑1 (MQ=255)
              cccATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTAcc                                                                                                                                    >  2:1342291/1‑150 (MQ=255)
              ccaaTGCGTAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGATTAAAACGCGAATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTAcc                                                                                                                                    <  1:5080694/147‑1 (MQ=255)
                cATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTAcccc                                                                                                                                  >  2:249924/1‑150 (MQ=255)
                 aTGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCa                                                                                                                                 <  2:2203998/150‑1 (MQ=255)
                 aTGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCa                                                                                                                                 <  2:1658075/150‑1 (MQ=255)
                  tGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCat                                                                                                                                <  1:4118922/150‑1 (MQ=255)
                    cGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCatat                                                                                                                              >  2:3706638/1‑150 (MQ=255)
                              cACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAgg                                                                                                                    <  1:2043804/150‑1 (MQ=255)
                                cGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCg                                                                                                                  >  2:3354528/1‑150 (MQ=255)
                                 gATGCCGAGAGACAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTCTCCAGACGCCCGCTCAGCAAAGAAGCATTGGCAAAAGCAAACGCCCCAGTGAAACCCACGTACCCCCTCGAACGCAGAGGCgg                                                                                                                 >  2:1987834/1‑150 (MQ=255)
                                  aTGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGa                                                                                                                >  1:3115398/1‑150 (MQ=255)
                                   tGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGAt                                                                                                               <  2:496270/150‑1 (MQ=255)
                                   tGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGAt                                                                                                               <  2:2636595/150‑1 (MQ=255)
                                    gCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATg                                                                                                              >  2:5263545/1‑150 (MQ=255)
                                     ccGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGAAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATgg                                                                                                             <  1:186829/150‑1 (MQ=255)
                                        aGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAg                                                                                                          <  2:2574793/150‑1 (MQ=255)
                                         gCGTCTGGATGATCCACGATGCCAGGGTACACGGACGATTAAAACGCTCATAATTGCTTTCCAGACGCCCGCTAATATATGATTCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGa                                                                                                         <  1:2879842/150‑1 (MQ=255)
                                          cGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGAt                                                                                                        >  1:2350405/1‑150 (MQ=255)
                                           gTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATc                                                                                                       >  1:4822444/1‑150 (MQ=255)
                                            tCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCCCGTACCCCATATACAGCAGAGGCGGATGGAAGATCa                                                                                                      <  2:682205/150‑1 (MQ=255)
                                            tCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGACTATCa                                                                                                      >  2:1904584/1‑150 (MQ=255)
                                            tCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCa                                                                                                      <  2:1778617/150‑1 (MQ=255)
                                            tCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCa                                                                                                      <  2:2647173/150‑1 (MQ=255)
                                            tCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCa                                                                                                      <  2:3227961/150‑1 (MQ=255)
                                            tCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCa                                                                                                      <  2:5067434/150‑1 (MQ=255)
                                             cAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAg                                                                                                     <  2:2988755/150‑1 (MQ=255)
                                                gAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGccc                                                                                                  <  1:5304297/150‑1 (MQ=255)
                                                 tagttCCTCGCTGCCTGCGTCCACGTACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGATCATCAAAGAAGCAATGGAAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGcccc                                                                                                 <  1:2198574/146‑1 (MQ=255)
                                                    aTCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGCTGGAAGATCAGCCCCGGa                                                                                              >  2:2970634/1‑150 (MQ=255)
                                                    aTCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGa                                                                                              >  2:882641/1‑150 (MQ=255)
                                                       cACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATcc                                                                                           <  2:1170827/150‑1 (MQ=255)
                                                         cGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTg                                                                                         >  1:476473/1‑150 (MQ=255)
                                                             gCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAAt                                                                                     <  2:492682/150‑1 (MQ=255)
                                                             gCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAAt                                                                                     >  1:4500928/1‑150 (MQ=255)
                                                              ccAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATa                                                                                    <  1:5005919/150‑1 (MQ=255)
                                                              ccAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATa                                                                                    <  2:4898343/150‑1 (MQ=255)
                                                                aGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGc                                                                                  >  2:2093285/1‑150 (MQ=255)
                                                                   gTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCggg                                                                               >  1:2211879/1‑150 (MQ=255)
                                                                    tCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGt                                                                              <  2:4615631/150‑1 (MQ=255)
                                                                       aCGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTaa                                                                           >  1:1046312/1‑150 (MQ=255)
                                                                        cGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCTATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCACCGGATCCTGCAATAGCGGGTTaat                                                                          <  2:1219934/150‑2 (MQ=255)
                                                                        cGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAg                                                                          <  1:1418719/150‑1 (MQ=255)
                                                                        cGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAg                                                                          >  2:1639974/1‑150 (MQ=255)
                                                                          gACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGAt                                                                        <  1:5171129/150‑1 (MQ=255)
                                                                          gACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGAt                                                                        <  2:5282627/150‑1 (MQ=255)
                                                                           aCGAGAAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATc                                                                       <  1:4058861/150‑1 (MQ=255)
                                                                              aGTAAAACGCGCATAATTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACCCCACAGAGAAACCCACTTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCTGGATCCTGCAATAGCGGGATAACATctcg                                                                    >  1:887345/1‑150 (MQ=255)
                                                                              aGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATcgcg                                                                    <  2:3499096/150‑1 (MQ=255)
                                                                              aGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATcgcg                                                                    >  2:1154931/1‑150 (MQ=255)
                                                                               gTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAATGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGa                                                                   >  2:3563845/1‑150 (MQ=255)
                                                                                tAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGAc                                                                  <  1:3803105/150‑1 (MQ=255)
                                                                                 aaaaCGCTCATATGTGCTGTCCATACGCCCGATCAGAAAATAAGAAATGGCAAAAGCAAACGCCACAGAGAAACCCACTTACCCCATATAAAGCAGAGGAGGATGGAAGATCTGCACCGTATCCTGCAATAGCGGGTTNAGATCGCGAcc                                                                 <  1:2767664/150‑1 (MQ=255)
                                                                                 aaaaCGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGAcc                                                                 <  1:3446964/150‑1 (MQ=255)
                                                                                    acgataaTAAGTGATGTCCATACGCCCTCTCAGAAAAGAAGAAATGGAAAAATAAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTc                                                              <  1:3628913/144‑1 (MQ=255)
                                                                                    aCGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTc                                                              <  1:1613496/150‑1 (MQ=255)
                                                                                      gcgcATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCaa                                                            <  1:1258796/150‑1 (MQ=255)
                                                                                       cgcATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAAt                                                           <  1:3030778/150‑1 (MQ=255)
                                                                                       cgcATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAAt                                                           <  1:3569673/150‑1 (MQ=255)
                                                                                       cgcATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAAt                                                           <  2:3853058/150‑1 (MQ=255)
                                                                                       cgcATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAAt                                                           >  2:438615/1‑150 (MQ=255)
                                                                                         cATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCg                                                         >  2:584062/1‑150 (MQ=255)
                                                                                          aTAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCgg                                                        <  1:4626093/150‑1 (MQ=255)
                                                                                          aTAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCgg                                                        >  1:2145413/1‑150 (MQ=255)
                                                                                          aTAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCgg                                                        >  1:1978714/1‑150 (MQ=255)
                                                                                            aaGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGa                                                      <  1:3429567/150‑1 (MQ=255)
                                                                                                 cTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTc                                                 <  2:5046748/150‑1 (MQ=255)
                                                                                                    tCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGc                                              <  2:406453/150‑1 (MQ=255)
                                                                                                       aGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAAc                                           <  1:2902676/150‑1 (MQ=255)
                                                                                                       aGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAAc                                           >  1:2513901/1‑150 (MQ=255)
                                                                                                        gACGCCCGATCAGCTCAGTTGCATTGGCATAGGCACACGCCACAGAGAAACCCACGTACCCCCTATCCAGCCGAGGCGGATGGTAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACg                                          <  1:4528474/150‑1 (MQ=255)
                                                                                                           gCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGACGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGc                                       <  2:3074341/150‑1 (MQ=255)
                                                                                                               gCTCATCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGATGCGGATGGAAGATCAGACCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCgaga                                   <  2:3983712/150‑1 (MQ=255)
                                                                                                               gCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCgaga                                   <  2:1011046/150‑1 (MQ=255)
                                                                                                                   aGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCgagagacc                               >  2:5148011/1‑148 (MQ=255)
                                                                                                                   aGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAAc                               >  1:3496524/1‑150 (MQ=255)
                                                                                                                   aGCAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAAc                               >  1:1553988/1‑150 (MQ=255)
                                                                                                                     cAAAGAAGCAATGGCAATAGCAAACGCCACAGAGAAACCCTCGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGAGGGTTAAGATCGCGACCTTCTATCGGGTAGTTCGGCAACGTGCGAGAGAACgg                             <  1:298551/150‑1 (MQ=255)
                                                                                                                     cAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACgg                             >  2:3203813/1‑150 (MQ=255)
                                                                                                                     cAAAGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACgg                             <  2:3022176/150‑1 (MQ=255)
                                                                                                                      aaaGAAGCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAATCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAATATCGAGACCTTCAATCGGGAAGTTCGGCAACGTGCTAGAGAACggg                            >  1:711031/1‑150 (MQ=255)
                                                                                                                        agaagCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGtt                          <  1:1033797/150‑1 (MQ=255)
                                                                                                                          aagCAATGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTag                        >  2:5118314/1‑150 (MQ=255)
                                                                                                                              aaTGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGt                    <  2:816085/150‑1 (MQ=255)
                                                                                                                                tGGCAAAAGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTaa                  <  1:2635137/150‑1 (MQ=255)
                                                                                                                                    aaaaGCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAagag              >  2:453416/1‑150 (MQ=255)
                                                                                                                                        gCAAACGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATg          <  2:3120962/150‑1 (MQ=255)
                                                                                                                                          aaaCGCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGACCGGGTTAGAGGTAAAGAGAATGaa        >  1:1801811/1‑150 (MQ=255)
                                                                                                                                          aaaCGCCACAGAGAAACCCACGTACACCATATAAAGCATAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGATCGGCAACTAGCGAGAGAACGGGTTAGAGGTACAGAGAATGaa        >  1:1224595/1‑150 (MQ=255)
                                                                                                                                              gCCACAGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGAACAGc    <  1:80656/150‑1 (MQ=255)
                                                                                                                                                cacaGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGAACAGCaa  >  1:1765330/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACGCCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAGATCGCGACCTTCAATCGGGAAGTTCGGCAACGTGCGAGAGAACGGGTTAGAGGTAAAGAGAATGAACAGCAA  >  CP009273/2287682‑2287975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑