Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,330,502 C→A 100% D800Y (GAT→TAT)  gyrA ← DNA gyrase (type II topoisomerase), subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,330,5020CA100.0% 1029.9 / NA 319D800Y (GAT→TAT) gyrADNA gyrase (type II topoisomerase), subunit A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (140/179);  total (140/179)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 319 total aligned reads displayed.

CCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAAC  >  CP009273/2330355‑2330648
                                                                                                                                                   |                                                                                                                                                  
ccAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAgg                                                                                                                                                  <  1:3828258/150‑1 (MQ=255)
  aGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTg                                                                                                                                                >  1:4609369/1‑150 (MQ=255)
  aGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTg                                                                                                                                                >  1:1954773/1‑150 (MQ=255)
    aTCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGat                                                                                                                                              >  1:4572006/1‑150 (MQ=255)
    aTCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGat                                                                                                                                              <  1:4017315/150‑1 (MQ=255)
     tCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGatc                                                                                                                                             <  1:3606900/150‑1 (MQ=255)
          cTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATcatcat                                                                                                                                        <  1:5315737/150‑1 (MQ=255)
           tCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATg                                                                                                                                       <  1:1003938/150‑1 (MQ=255)
             gTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGAt                                                                                                                                     <  2:2432787/150‑1 (MQ=255)
               cAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCt                                                                                                                                   >  2:1618571/1‑150 (MQ=255)
                   cGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTc                                                                                                                               <  1:1781131/150‑1 (MQ=255)
                   cGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTc                                                                                                                               >  1:2051131/1‑150 (MQ=255)
                    ggTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCg                                                                                                                              <  2:2069725/150‑1 (MQ=255)
                     gTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGc                                                                                                                             <  2:3479892/150‑1 (MQ=255)
                         aGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCGCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTc                                                                                                                         >  1:4695957/1‑150 (MQ=255)
                         aGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTc                                                                                                                         >  2:4034266/1‑150 (MQ=255)
                         aGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTc                                                                                                                         >  2:4494942/1‑150 (MQ=255)
                           cAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCAt                                                                                                                       <  2:2301376/150‑1 (MQ=255)
                            aaCACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCCGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGTTCGCAGTCATc                                                                                                                      >  2:2626148/1‑150 (MQ=255)
                             acccGTTGCAGACCCACTCCGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCt                                                                                                                     <  2:2237249/147‑1 (MQ=255)
                             acacGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCt                                                                                                                     <  1:4338680/150‑1 (MQ=255)
                             acacGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCt                                                                                                                     <  2:779248/150‑1 (MQ=255)
                               acGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTAc                                                                                                                   >  2:919630/1‑150 (MQ=255)
                               acGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTAc                                                                                                                   >  1:3602528/1‑150 (MQ=255)
                               acGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTAc                                                                                                                   <  2:3015805/150‑1 (MQ=255)
                                cGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTAcc                                                                                                                  <  1:2571295/150‑1 (MQ=255)
                                   tGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGt                                                                                                               >  2:180694/1‑150 (MQ=255)
                                     cAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCCTCTACCTGTAc                                                                                                             >  2:4256866/1‑150 (MQ=255)
                                      aGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTAcc                                                                                                            >  2:585603/1‑150 (MQ=255)
                                      aGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTAcc                                                                                                            <  2:2172780/150‑1 (MQ=255)
                                      aGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTAcc                                                                                                            <  2:1892354/150‑1 (MQ=255)
                                      aGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTAcc                                                                                                            >  2:3965870/1‑150 (MQ=255)
                                      aGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTAcc                                                                                                            >  2:1390655/1‑150 (MQ=255)
                                            aCTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCca                                                                                                      <  2:5248668/150‑1 (MQ=255)
                                                  ttttCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACt                                                                                                >  1:32082/1‑150 (MQ=255)
                                                    ttCATCTTCCGCAGTACGGATGAGGAGCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTaa                                                                                              >  2:1128454/1‑150 (MQ=255)
                                                     tCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTaaa                                                                                             >  1:2669064/1‑150 (MQ=255)
                                                     tCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTaaa                                                                                             >  2:4470399/1‑150 (MQ=255)
                                                       aTCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATGTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAAcc                                                                                           <  1:5157025/150‑1 (MQ=255)
                                                       aNCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGAGCATCATGATCTGGGCGCAGTCATCTACCTGTACCGCGCCAACAACTAAAcc                                                                                           >  1:3188318/1‑150 (MQ=255)
                                                         cTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGt                                                                                         >  2:153434/1‑150 (MQ=255)
                                                          ttCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGtt                                                                                        >  2:3724563/1‑150 (MQ=255)
                                                            ccGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTAc                                                                                      <  1:724652/150‑1 (MQ=255)
                                                            ccGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTAc                                                                                      <  1:2122798/150‑1 (MQ=255)
                                                            ccGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTAc                                                                                      <  1:4410257/150‑1 (MQ=255)
                                                             cGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACg                                                                                     >  1:3648664/1‑150 (MQ=255)
                                                                aGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTc                                                                                  <  1:5234488/150‑1 (MQ=255)
                                                                aGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTc                                                                                  <  2:4604746/150‑1 (MQ=255)
                                                                aGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTc                                                                                  >  1:3305413/1‑150 (MQ=255)
                                                                aGTACGGATGAGGATCACGCAATGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCTTACAGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTTTACCGCGCCTACAACTAAACCGTTACGTTc                                                                                  <  1:4370284/150‑1 (MQ=255)
                                                                  tACGGATGAGGATCACGCCCTGGGTGTTTCGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCgg                                                                                <  2:3576214/150‑1 (MQ=255)
                                                                   aCGGATGAGGATCACGCCCTGGGTGTTACGGCCCAAGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGt                                                                               <  2:2360662/150‑1 (MQ=255)
                                                                    cGGAAGAGGATCACGCCCTGGGTGTTACGGCCCACGAAGCAGATTTCCGAAACGCGAGTACGTACCAGCGAACCGGCATAGGAGATCATCAAGAACAGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGAAACGTTCGGTa                                                                              >  2:4805677/1‑150 (MQ=255)
                                                                     ggATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTaa                                                                             <  2:1423277/150‑1 (MQ=255)
                                                                       aTGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAAcc                                                                           >  1:1367095/1‑150 (MQ=255)
                                                                         gAGGATCACGCCCTGGGTGTTACGGCCCACGATGATGATTTCCGAACCGCGAGTACGTACCAGCGTACCGTCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCtt                                                                         <  1:4950787/150‑1 (MQ=255)
                                                                           ggATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGa                                                                       >  1:3551815/1‑150 (MQ=255)
                                                                            gATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGAt                                                                      <  2:269510/150‑1 (MQ=255)
                                                                            gATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGAt                                                                      <  2:5199120/150‑1 (MQ=255)
                                                                                aCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGgag                                                                  <  2:865141/150‑1 (MQ=255)
                                                                                 cGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGgaga                                                                 >  1:4031141/1‑150 (MQ=255)
                                                                                  gCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTTCCAGCGTACCGGCAGAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGAt                                                                <  2:269012/150‑1 (MQ=255)
                                                                                  gCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGAt                                                                <  1:4802204/150‑1 (MQ=255)
                                                                                  gCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGAt                                                                >  1:1833292/1‑150 (MQ=255)
                                                                                          tgtTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCTGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCtt                                                        >  2:3400359/1‑150 (MQ=255)
                                                                                             tACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTtcg                                                     >  2:3935574/1‑150 (MQ=255)
                                                                                               cGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTtcgtc                                                   >  1:4509685/1‑150 (MQ=255)
                                                                                                ggCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTtcgtcg                                                  <  1:2376843/150‑1 (MQ=255)
                                                                                                      cGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAcgcg                                            <  1:149875/150‑1 (MQ=255)
                                                                                                         tGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACt                                         >  1:5214009/1‑150 (MQ=255)
                                                                                                         tGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACt                                         >  1:412840/1‑150 (MQ=255)
                                                                                                          gCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACtt                                        <  2:2417642/150‑1 (MQ=255)
                                                                                                            tGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttgg                                      >  2:694649/1‑150 (MQ=255)
                                                                                                            tGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttgg                                      <  2:4905779/150‑1 (MQ=255)
                                                                                                             gATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttggt                                     >  2:2571244/1‑150 (MQ=255)
                                                                                                               tttCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAACCCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttggttg                                   <  2:3273782/150‑1 (MQ=255)
                                                                                                                ttCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttggttgg                                  <  1:4916572/150‑1 (MQ=255)
                                                                                                                ttCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttggttgg                                  <  1:2717761/150‑1 (MQ=255)
                                                                                                                  ncGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGt                                >  1:2857303/2‑150 (MQ=255)
                                                                                                                  ccGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGt                                >  2:2514331/1‑150 (MQ=255)
                                                                                                                    gAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTAt                              <  1:3384064/150‑1 (MQ=255)
                                                                                                                    gAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTAt                              <  2:40104/150‑1 (MQ=255)
                                                                                                                      aaCGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTc                            <  1:1869482/150‑1 (MQ=255)
                                                                                                                            aGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCAc                      >  2:2901835/1‑150 (MQ=255)
                                                                                                                            aGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCAc                      >  1:1605465/1‑150 (MQ=255)
                                                                                                                             gtacgtacCAGCGTACCGGCATAGGTGATCAGCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAACCCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACt                     <  2:4838314/150‑1 (MQ=255)
                                                                                                                              tacgtacCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTg                    >  2:3770750/1‑150 (MQ=255)
                                                                                                                              tacgtacCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTg                    <  2:3461427/150‑1 (MQ=255)
                                                                                                                              tacgtacCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTg                    >  1:3070747/1‑150 (MQ=255)
                                                                                                                                 gtacCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCgg                 <  2:2001559/150‑1 (MQ=255)
                                                                                                                                 gtacCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCgg                 >  1:594498/1‑150 (MQ=255)
                                                                                                                                 gtacCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCgg                 >  1:2239179/1‑150 (MQ=255)
                                                                                                                                 gtacCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCgg                 >  1:4664503/1‑150 (MQ=255)
                                                                                                                                  tacCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGt                <  1:418905/150‑1 (MQ=255)
                                                                                                                                     cAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACg             >  1:636169/1‑150 (MQ=255)
                                                                                                                                     cAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACg             <  1:3724638/150‑1 (MQ=255)
                                                                                                                                      aGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGt            >  2:1230873/1‑150 (MQ=255)
                                                                                                                                           aCCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTAcc       <  2:4885946/150‑1 (MQ=255)
                                                                                                                                           aCCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTAcc       <  1:1340844/150‑1 (MQ=255)
                                                                                                                                                cATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAAc  <  1:5065404/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAAC  >  CP009273/2330355‑2330648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑