Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,334,722 C→A 10.4% R976L (CGA→CTA)  yfaL ← adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,334,7220CA10.4% 888.8 / 47.4 263R976L (CGA→CTA) yfaLadhesin
Reads supporting (aligned to +/- strand):  ref base C (106/129);  new base A (14/14);  total (120/143)
Fisher's exact test for biased strand distribution p-value = 6.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 266 total aligned reads displayed.

TGCCCCAGCGCCCGCTAAACAGATCGCCGCTAAGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCAGGATCCGGCGTGGGATCAGGATCGGGAGTCGGGTCT  >  CP009273/2334573‑2334871
                                                                                                                                                     |                                                                                                                                                     
tGCCCCAGCGCCCGCTAAACAGATCGCCGCTAAGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTc                                                                                                                                                       <  1:1817597/150‑1 (MQ=255)
    ccAGCGCCCGCTAAACAGATCGCCGCTAAGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTc                                                                                                                                                   <  1:3107132/150‑1 (MQ=255)
    ccAGCGCCCGCTAAACAGATCGCCGCTAAGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTc                                                                                                                                                   <  1:3107146/150‑1 (MQ=255)
     cAGCGCCCGCTAAACAGATCGCCGCTAAGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTcc                                                                                                                                                  >  2:5226042/1‑150 (MQ=255)
      aGCGCCCGCTAAACAGATCGCCGCTAAGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCca                                                                                                                                                 >  2:2011462/1‑150 (MQ=255)
          cccGCTAAACAGATCGCCGCTAAGTTGCACCGTAGAAGTGTCTTCATGGTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCAGCGTGATCGCGTAGCTCcagcag                                                                                                                                             >  1:3448451/1‑149 (MQ=255)
            cGCTAAACAGATCGCCGCTAAGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTAGCTCCATCATaa                                                                                                                                           >  2:1728198/1‑150 (MQ=255)
               tAAACAGATCGCCGCTAAGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACg                                                                                                                                        <  2:2209193/150‑1 (MQ=255)
                aaaCAGATCGCCGCTAAGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGc                                                                                                                                       >  2:180970/1‑150 (MQ=255)
                  aCAGATCGCCGCTAAGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCt                                                                                                                                     <  1:4978368/150‑1 (MQ=255)
                  aCAGATCGCCGCTAAGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTAGCTCCATCATAAACGCCt                                                                                                                                     <  2:3608185/150‑1 (MQ=255)
                     gATCGCCGCTAAGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGAt                                                                                                                                  <  1:3281394/150‑1 (MQ=255)
                      aTCGCCGCTAAGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTACGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGAtt                                                                                                                                 >  1:300975/1‑150 (MQ=255)
                       tCGCCGCTAAGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGAttt                                                                                                                                <  1:1688482/150‑1 (MQ=255)
                       tCGCCGCTAAGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGAttt                                                                                                                                <  1:1947497/150‑1 (MQ=255)
                       tCGCCGCTAAGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGAttt                                                                                                                                <  2:1666903/150‑1 (MQ=255)
                        cgccgcTAAGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTg                                                                                                                               >  1:1424111/1‑150 (MQ=255)
                        cgccgcTAAGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTg                                                                                                                               <  1:1216410/150‑1 (MQ=255)
                            gcTAAGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTgccgc                                                                                                                           >  2:2885356/1‑150 (MQ=255)
                            gcTAAGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGAGTTgccgc                                                                                                                           >  1:3510355/1‑150 (MQ=255)
                             cTAAGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTgccgcc                                                                                                                          <  1:255809/150‑1 (MQ=255)
                                aGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGccgcccgc                                                                                                                       <  2:4456663/150‑1 (MQ=255)
                                  ttGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAg                                                                                                                     <  1:3834471/150‑1 (MQ=255)
                                    gCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTAGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGt                                                                                                                   <  1:2936034/150‑1 (MQ=255)
                                      acggTAGATGGGTCTTCTTGTTGAGCCTGTGGCCCCGCTGTTGTGTAATTATAATCTCCGCCGATAACACGTAATTTCAGCGTCTGACCATCGCCACCTGCGTGATCGGGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCTGTtta                                                                                                                 <  2:1515695/147‑1 (MQ=255)
                                      aCCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGtta                                                                                                                 <  2:4986398/150‑1 (MQ=255)
                                        cGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGttatt                                                                                                               <  1:3020127/150‑1 (MQ=255)
                                        cGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGttatt                                                                                                               >  1:4678600/1‑150 (MQ=255)
                                           aGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTAGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAg                                                                                                            >  1:4729220/1‑150 (MQ=255)
                                             aaGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGAt                                                                                                          <  1:2859927/150‑1 (MQ=255)
                                             aaGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGAt                                                                                                          <  2:1831590/150‑1 (MQ=255)
                                             aaGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGAt                                                                                                          <  2:3384331/150‑1 (MQ=255)
                                              aGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATa                                                                                                         <  2:1206856/150‑1 (MQ=255)
                                              aGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCAAAAACGCCTGATTTGCCGCCCGCAGGTTATAAAGATa                                                                                                         >  2:3638453/1‑150 (MQ=255)
                                                tgtCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAAc                                                                                                       >  2:2343990/1‑150 (MQ=255)
                                                   cTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGc                                                                                                    >  1:5146668/1‑150 (MQ=255)
                                                    ttCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATACACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGcc                                                                                                   <  1:1849594/150‑1 (MQ=255)
                                                       aTGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAAc                                                                                                >  1:1401051/1‑150 (MQ=255)
                                                       aTGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTAGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAAc                                                                                                >  1:816623/1‑150 (MQ=255)
                                                        tGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACt                                                                                               <  2:5147299/150‑1 (MQ=255)
                                                            gAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTgg                                                                                           >  1:3614017/1‑150 (MQ=255)
                                                             aGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGc                                                                                          <  2:862840/150‑1 (MQ=255)
                                                                 aGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTc                                                                                      >  1:3044643/1‑150 (MQ=255)
                                                                     gCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAaca                                                                                  >  2:2249550/1‑150 (MQ=255)
                                                                        ccGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCg                                                                               <  1:1776528/150‑1 (MQ=255)
                                                                        ccGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCg                                                                               <  2:3451547/150‑1 (MQ=255)
                                                                         cGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTAAACACCgg                                                                              >  1:1574003/1‑150 (MQ=255)
                                                                          gctgctGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGc                                                                             >  2:1912826/1‑150 (MQ=255)
                                                                              ctgTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGt                                                                         <  1:2108479/150‑1 (MQ=255)
                                                                              ctgTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGt                                                                         >  1:3396310/1‑150 (MQ=255)
                                                                              ctgTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGt                                                                         >  1:555693/1‑150 (MQ=255)
                                                                              ctgTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTAGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGt                                                                         <  2:3975310/150‑1 (MQ=255)
                                                                               tgtgTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTa                                                                        <  1:1036890/150‑1 (MQ=255)
                                                                               tgtgTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTa                                                                        <  1:4380149/150‑1 (MQ=255)
                                                                                  gtAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCACCAACTTTGGCATTCAACACCGGCTGGTAAGc                                                                     <  2:417898/150‑1 (MQ=255)
                                                                                    aaTGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAg                                                                   <  2:1455719/150‑1 (MQ=255)
                                                                                    aaTGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAg                                                                   <  2:4118947/150‑1 (MQ=255)
                                                                                     aTGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAgg                                                                  <  2:2627550/150‑1 (MQ=255)
                                                                                      tGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGc                                                                 <  1:1123095/150‑1 (MQ=255)
                                                                                        aTAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGt                                                               >  1:526027/1‑150 (MQ=255)
                                                                                        aTAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGt                                                               >  1:501114/1‑150 (MQ=255)
                                                                                        aTAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTAGCTCCATCATAAACGCCTGATTTGCCGCACGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGt                                                               >  1:2615082/1‑150 (MQ=255)
                                                                                          aaTCTCCGCCGATAACACGTAAATTCATAGTCTTACCATCGCCAACTGCGTGATAGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACAACGGCTGGTAAGCAGGCGAAg                                                             >  1:804288/1‑150 (MQ=255)
                                                                                            tctcCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGt                                                           <  1:1314627/150‑1 (MQ=255)
                                                                                              tcCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTc                                                         <  2:3120727/150‑1 (MQ=255)
                                                                                               ccgccgATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTcc                                                        <  2:2901149/150‑1 (MQ=255)
                                                                                               ccgccgATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCACCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTcc                                                        <  2:1028781/150‑1 (MQ=255)
                                                                                                cgccgATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCcg                                                       >  2:2781309/1‑150 (MQ=255)
                                                                                                 gccgATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCcgg                                                      >  2:2246967/1‑150 (MQ=255)
                                                                                                   cgATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCcgggt                                                    >  2:5210684/1‑150 (MQ=255)
                                                                                                    gATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCcgggtc                                                   >  1:2383012/1‑150 (MQ=255)
                                                                                                    gATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTAGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCcgggtc                                                   <  2:4407474/150‑1 (MQ=255)
                                                                                                     aTAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCcgggtcg                                                  >  2:1157631/1‑150 (MQ=255)
                                                                                                      tAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCcgggtcgg                                                 >  1:355034/1‑150 (MQ=255)
                                                                                                         cacGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGgtg                                              >  1:3934060/1‑150 (MQ=255)
                                                                                                         cacGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCACGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACAGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGgtg                                              >  1:117743/1‑150 (MQ=255)
                                                                                                           cGTAAATACAGCGACTGACCCTCGCCACCTCCGTGATCGCGTCGCTCCATCAGAAACGCCTGATTTGCCGCCCGCAGGTGATTCAGATAACCGCCACCTTTGGCATTCAACACCCGCTGGTAAGCAGGCGTCGGTTCCGGGTCGGGTGtt                                            >  2:322693/1‑150 (MQ=255)
                                                                                                             tAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTgg                                          <  1:482621/150‑1 (MQ=255)
                                                                                                              aaaTTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGa                                         >  2:4872650/1‑150 (MQ=255)
                                                                                                                 ttCAGCTTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCAACATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCa                                      <  1:2303707/150‑1 (MQ=255)
                                                                                                                 ttCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCa                                      <  2:4559734/150‑1 (MQ=255)
                                                                                                                 ttCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCa                                      <  2:4102099/150‑1 (MQ=255)
                                                                                                                      cGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCAGGATc                                 <  1:4768481/150‑1 (MQ=255)
                                                                                                                      cGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCAGGATc                                 <  1:4087793/150‑1 (MQ=255)
                                                                                                                          tGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCAGGATCCGGc                             >  2:3062251/1‑150 (MQ=255)
                                                                                                                           gACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATATACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCAGGATCCGGCg                            >  1:5280793/1‑150 (MQ=255)
                                                                                                                           gACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCAGGATCCGGCg                            >  2:1575364/1‑150 (MQ=255)
                                                                                                                                tCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCAGGATCCGGCGTGGGa                       <  1:243982/150‑1 (MQ=255)
                                                                                                                                  gCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCAGGATCCGGCGTGGGATc                     <  2:1003292/150‑1 (MQ=255)
                                                                                                                                     aCCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCAGGATCCGGCGTGGGATCAgg                  <  2:3565709/150‑1 (MQ=255)
                                                                                                                                       cTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATGTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCAGGATCCGGCGTGGGATCAGGAt                <  2:3866559/150‑1 (MQ=255)
                                                                                                                                         gCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCAGGATCCGGCGTGGGATCAGGATCg              >  2:3889940/1‑150 (MQ=255)
                                                                                                                                          cGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCAGGATCCGGCGTGGGATCAGGATCgg             <  1:2011462/150‑1 (MQ=255)
                                                                                                                                          cGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCAGGATCCGGCGTGGGATCAGGATCgg             >  1:4643375/1‑150 (MQ=255)
                                                                                                                                           gTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCAGGATCCGGCGTGGGATCAGGATCggg            >  1:1077083/1‑150 (MQ=255)
                                                                                                                                            tGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCAGGATCCGGCGTGGGATCAGGATCGGGa           <  2:1171874/150‑1 (MQ=255)
                                                                                                                                             gATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCAGGATCCGGCGTGGGATCAGGATCGGGAg          >  1:4500819/1‑150 (MQ=255)
                                                                                                                                             gATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCAGGATCCGGCGTGGGATCAGGATCGGGAg          >  2:2437637/1‑150 (MQ=255)
                                                                                                                                               tCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCAGGATCCGGCGTGGGATCAGGATCGGGAGTc        <  2:3697720/150‑1 (MQ=255)
                                                                                                                                                  cgTCGCACCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCAGGATCCGGCGTGGGATCAGGATCGGGAGTCggg     >  2:2585942/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCCCCAGCGCCCGCTAAACAGATCGCCGCTAAGTTGCACCGTAGAAGTGTCTTCATGTTGAGCCAGTTGCCCCGCTGCTGTGTAATGATAATCTCCGCCGATAACACGTAAATTCAGCGTCTGACCATCGCCACCTGCGTGATCGCGTCGCTCCATCATAAACGCCTGATTTGCCGCCCGCAGGTTATTAAGATAACCGCCAACTTTGGCATTCAACACCGGCTGGTAAGCAGGCGTAGGTTCCGGGTCGGGTGTTGGATCAGGATCCGGCGTGGGATCAGGATCGGGAGTCGGGTCT  >  CP009273/2334573‑2334871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑