Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,358,641 G→T 100% I153I (ATC→ATA)  ais ← putative LPS core heptose(II)‑phosphate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,358,6410GT99.7% 1032.4 / ‑4.6 314I153I (ATC→ATA) aisputative LPS core heptose(II)‑phosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (172/141);  total (172/142)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 319 total aligned reads displayed.

AATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTGGA  >  CP009273/2358494‑2358787
                                                                                                                                                   |                                                                                                                                                  
aaTTTATTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTat                                                                                                                                                  >  2:3946319/1‑150 (MQ=255)
aaTTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTat                                                                                                                                                  <  1:4878748/150‑1 (MQ=255)
 aTTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACtata                                                                                                                                                 <  2:1741516/150‑1 (MQ=255)
 aTTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACtata                                                                                                                                                 <  1:4778268/150‑1 (MQ=255)
 aTTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACtata                                                                                                                                                 <  2:3585771/150‑1 (MQ=255)
   ttAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATAtt                                                                                                                                               <  1:2630443/150‑1 (MQ=255)
     aGTGGTTAACGAAGTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATAtttt                                                                                                                                             <  1:3869631/150‑1 (MQ=255)
      gTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATAttttt                                                                                                                                            <  2:3168020/150‑1 (MQ=255)
      gTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATAttttt                                                                                                                                            <  2:4937350/150‑1 (MQ=255)
        ggTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTAt                                                                                                                                          <  1:5300564/150‑1 (MQ=255)
         gTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATc                                                                                                                                         >  2:1355498/1‑150 (MQ=255)
         gTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATc                                                                                                                                         <  1:2987142/150‑1 (MQ=255)
         gTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATc                                                                                                                                         <  2:5194641/150‑1 (MQ=255)
          ttAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGATTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCa                                                                                                                                        <  2:14411/150‑1 (MQ=255)
           tAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAg                                                                                                                                       <  2:2023362/150‑1 (MQ=255)
            aaCGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAgg                                                                                                                                      >  1:371901/1‑150 (MQ=255)
            aaCGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAgg                                                                                                                                      <  2:2222656/150‑1 (MQ=255)
            aaCGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAgg                                                                                                                                      >  1:3291571/1‑150 (MQ=255)
            aaCGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAgg                                                                                                                                      <  1:2698747/150‑1 (MQ=255)
              cGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGgcg                                                                                                                                    >  1:746350/1‑150 (MQ=255)
               gAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGgcgc                                                                                                                                   >  1:3231907/1‑150 (MQ=255)
                aaTTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCgct                                                                                                                                  >  2:4065664/1‑150 (MQ=255)
                  gtCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCgcttt                                                                                                                                <  2:2379275/149‑1 (MQ=255)
                    ccccATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCgctttgc                                                                                                                              >  1:5104762/1‑150 (MQ=255)
                        aTCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGctttgctttg                                                                                                                          >  2:3783523/1‑150 (MQ=255)
                        aTCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGctttgctttg                                                                                                                          >  1:2874706/1‑150 (MQ=255)
                        aTCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGctttgctttg                                                                                                                          >  1:2030775/1‑150 (MQ=255)
                          ccAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTa                                                                                                                        >  1:4605451/1‑150 (MQ=255)
                           cAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTaa                                                                                                                       >  2:740792/1‑150 (MQ=255)
                            aGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCACTGATTATGGGTGAAAATAACTCTATTTTTATCAGGCGCTTTGCGTTGTAAg                                                                                                                      >  1:1462301/1‑150 (MQ=255)
                            aGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAg                                                                                                                      >  2:1039436/1‑150 (MQ=255)
                            aGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAg                                                                                                                      <  2:1660800/150‑1 (MQ=255)
                              aTAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTc                                                                                                                    >  1:1788953/1‑150 (MQ=255)
                               tAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTcc                                                                                                                   <  1:2512298/150‑1 (MQ=255)
                               tAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTGTAAATGTCGCGTCACGCTTATCTGTAGCAATATATGTCAGGCAATGAGTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTGTGTAAGTcc                                                                                                                   >  2:2747376/1‑150 (MQ=255)
                                aaaCTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGTCGCTTTGCTTTGTAAGTcat                                                                                                                  >  1:4480136/1‑148 (MQ=255)
                                aaaCTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCt                                                                                                                  >  2:822209/1‑150 (MQ=255)
                                aaaCTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCt                                                                                                                  <  1:5034178/150‑1 (MQ=255)
                                    tttGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAAt                                                                                                              <  2:2325088/150‑1 (MQ=255)
                                      tGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTg                                                                                                            <  2:4771832/150‑1 (MQ=255)
                                        ccTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCa                                                                                                          >  2:4201278/1‑150 (MQ=255)
                                        ccTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCa                                                                                                          <  2:1939814/150‑1 (MQ=255)
                                             ttCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACtata                                                                                                     >  2:3515149/1‑150 (MQ=255)
                                                       atgACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTa                                                                                           >  1:3136329/1‑150 (MQ=255)
                                                        tgACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTAc                                                                                          >  1:1623758/1‑150 (MQ=255)
                                                         gACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTAcc                                                                                         <  1:5170698/150‑1 (MQ=255)
                                                          aCTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCg                                                                                        >  2:5037852/1‑150 (MQ=255)
                                                          aCTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCg                                                                                        >  2:3683049/1‑150 (MQ=255)
                                                          aCTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCg                                                                                        >  2:4327823/1‑150 (MQ=255)
                                                             aaaCCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCAc                                                                                     >  2:4753779/1‑150 (MQ=255)
                                                              aaCCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACt                                                                                    <  1:4091011/150‑1 (MQ=255)
                                                               aCCATCCAGATAATCAGGTTTAACTGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGACAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTg                                                                                   <  1:1058994/150‑1 (MQ=255)
                                                               aCCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTg                                                                                   <  2:2688753/150‑1 (MQ=255)
                                                                 cATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTgaa                                                                                 <  1:1500965/150‑1 (MQ=255)
                                                                      aGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGt                                                                            >  2:1572794/1‑150 (MQ=255)
                                                                       gATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCTATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTc                                                                           <  2:655870/150‑1 (MQ=255)
                                                                        aTAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCg                                                                          >  2:3902406/1‑150 (MQ=255)
                                                                          aaTCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGtt                                                                        <  1:4441368/150‑1 (MQ=255)
                                                                            tCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTa                                                                      <  2:307757/150‑1 (MQ=255)
                                                                            tCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTa                                                                      <  2:2606008/150‑1 (MQ=255)
                                                                             cAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTAt                                                                     >  1:2853873/1‑150 (MQ=255)
                                                                             cAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTAt                                                                     <  2:3310947/150‑1 (MQ=255)
                                                                             cAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTAt                                                                     >  2:2985138/1‑150 (MQ=255)
                                                                             cAGGTTTAAATGTCGCGTCACGATTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTAt                                                                     <  1:897516/150‑1 (MQ=255)
                                                                               ggTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCTATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCt                                                                   <  1:4308974/150‑1 (MQ=255)
                                                                                gTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTa                                                                  <  2:345426/150‑1 (MQ=255)
                                                                                  ttAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTAcc                                                                >  1:1007505/1‑150 (MQ=255)
                                                                                     aaTGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTc                                                             >  1:2803086/1‑150 (MQ=255)
                                                                                            cgTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAAttttt                                                      <  2:4309416/150‑1 (MQ=255)
                                                                                             gTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAAtttttt                                                     <  2:5087862/150‑1 (MQ=255)
                                                                                             gTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAAtttttt                                                     >  1:5022771/1‑150 (MQ=255)
                                                                                                  gCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCg                                                >  2:3280257/1‑150 (MQ=255)
                                                                                                  gCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCg                                                <  1:2593253/150‑1 (MQ=255)
                                                                                                    ttATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCt                                              >  1:538522/1‑150 (MQ=255)
                                                                                                     tATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTg                                             <  2:2309867/150‑1 (MQ=255)
                                                                                                     tATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTg                                             >  1:2407431/1‑150 (MQ=255)
                                                                                                     tATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTg                                             >  1:1227643/1‑150 (MQ=255)
                                                                                                       tCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGaa                                           <  1:1944726/150‑1 (MQ=255)
                                                                                                              cAATATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAgg                                    >  1:763236/1‑150 (MQ=255)
                                                                                                               aaTATATGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGt                                   >  2:1325467/1‑150 (MQ=255)
                                                                                                                  atatGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGc                                <  2:1870380/150‑1 (MQ=255)
                                                                                                                    atGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCg                              <  2:1788953/150‑1 (MQ=255)
                                                                                                                     tGTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGa                             <  1:3336233/150‑1 (MQ=255)
                                                                                                                      gTCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGAc                            <  2:4478930/150‑1 (MQ=255)
                                                                                                                       tCAGGCAATGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACt                           <  1:970981/150‑1 (MQ=255)
                                                                                                                             aaTGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATgg                     <  2:2438658/150‑1 (MQ=255)
                                                                                                                              aTGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGt                    >  1:3916425/1‑150 (MQ=255)
                                                                                                                              aTGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGt                    >  1:1300813/1‑150 (MQ=255)
                                                                                                                              aTGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGt                    >  2:2473183/1‑150 (MQ=255)
                                                                                                                               tGATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTc                   >  2:4053133/1‑150 (MQ=255)
                                                                                                                                gATTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTcc                  <  1:2750600/150‑1 (MQ=255)
                                                                                                                                 aTTATGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCg                 <  1:4201278/150‑1 (MQ=255)
                                                                                                                                    aTGGGTGAAAATAACTATATTTTTATCTGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGAc              <  2:5279037/150‑1 (MQ=255)
                                                                                                                                    aTGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGAc              >  2:4159513/1‑150 (MQ=255)
                                                                                                                                     tGGGTGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACg             >  2:4587875/1‑150 (MQ=255)
                                                                                                                                         tGAAAATAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTAACCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTAt         >  1:4177018/1‑150 (MQ=255)
                                                                                                                                           aaaaTAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAAATTTTTACCCGCTGAAAACCCGGTAGCCGACTGAAGGGGCCGGACCGTATTa       >  1:1372336/1‑150 (MQ=255)
                                                                                                                                            aaaTAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTAc      >  1:2167167/1‑150 (MQ=255)
                                                                                                                                             aaTAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACt     <  1:2308282/150‑1 (MQ=255)
                                                                                                                                               tAACTATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTgg   <  2:4272762/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAATGATTATGGGTGAAAATAACGATATTTTTATCAGGCGCTTTGCTTTGTAAGTCCTTAATTGCACTATAAATCTCATTACCGCACTGAAGAAGTCGTTTATCTACCGTCAATTTTTTACCCGCTGAAAACCAGGTAGCCGACTGAATGGTCCGGACGGTATTACTGGA  >  CP009273/2358494‑2358787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑