Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,405,946 T→G 100% Q43P (CAG→CCG)  yfbU ← UPF0304 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,405,9460TG99.7% 1274.9 / ‑5.5 324Q43P (CAG→CCG) yfbUUPF0304 family protein
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base G (163/157);  total (165/159)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 324 total aligned reads displayed.

AAGGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACT  >  CP009273/2405799‑2406092
                                                                                                                                                   |                                                                                                                                                  
aaGGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACAACTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAAATCACGCATCGGt                                                                                                                                                  >  2:3152874/1‑150 (MQ=255)
   gTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAAt                                                                                                                                               <  2:176992/150‑1 (MQ=255)
   gTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAAt                                                                                                                                               <  1:3543662/150‑1 (MQ=255)
     gACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATcc                                                                                                                                             <  1:1324122/150‑1 (MQ=255)
       cacGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGt                                                                                                                                           >  1:3726683/1‑150 (MQ=255)
        acgacgTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTa                                                                                                                                          <  2:3819713/150‑1 (MQ=255)
        acgacgTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTa                                                                                                                                          >  2:3446615/1‑150 (MQ=255)
         agacgTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGAAACGCATGATACATCTACATAATTACGATGAAAGTGCGGCAGTTTTATTCTTTCAGCTCGCCAAACTCGCGATACAATTCAAGCAACGGAAATCCGaaa                                                                                                                                         >  2:425480/2‑150 (MQ=255)
           acgTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAAcc                                                                                                                                       >  1:5161264/1‑150 (MQ=255)
             gTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCAc                                                                                                                                     <  2:239678/150‑1 (MQ=255)
              ttCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGAAATCCGTAACCACg                                                                                                                                    >  2:1625022/1‑150 (MQ=255)
               tCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGc                                                                                                                                   >  2:3072268/1‑150 (MQ=255)
               tCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGc                                                                                                                                   >  2:4352675/1‑150 (MQ=255)
                cATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCt                                                                                                                                  >  2:2739949/1‑150 (MQ=255)
                cATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCt                                                                                                                                  <  2:2007964/150‑1 (MQ=255)
                cATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCAAATTCACGCATCGGTAATCCGTAACCACGCt                                                                                                                                  >  1:4313185/1‑150 (MQ=255)
                   cGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCaa                                                                                                                               >  1:1007428/1‑150 (MQ=255)
                   cGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCaa                                                                                                                               <  1:2161893/150‑1 (MQ=255)
                         aTTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTg                                                                                                                         >  2:3828792/1‑150 (MQ=255)
                            gCTGGTCCTGCATATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCTCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGttt                                                                                                                      <  1:3155001/150‑1 (MQ=255)
                              tGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGc                                                                                                                    >  2:3298495/1‑150 (MQ=255)
                                gTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAg                                                                                                                  <  2:3411497/150‑1 (MQ=255)
                                    tGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAAGAATTGTTTGCAGGCGa                                                                                                              >  1:1272831/1‑150 (MQ=255)
                                      cAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACg                                                                                                            >  2:4810553/1‑150 (MQ=255)
                                      cAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACg                                                                                                            <  1:2793175/150‑1 (MQ=255)
                                          ttAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCCCGCTCAATAATTGTTTGCAGGCGACGGTaa                                                                                                        >  1:2270695/1‑150 (MQ=255)
                                           tAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAAc                                                                                                       >  2:3678127/1‑150 (MQ=255)
                                            aGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACg                                                                                                      >  2:3943618/1‑150 (MQ=255)
                                            aGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACg                                                                                                      <  2:4125894/150‑1 (MQ=255)
                                            aGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACg                                                                                                      >  2:2502258/1‑150 (MQ=255)
                                             gACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGt                                                                                                     >  2:2160107/1‑150 (MQ=255)
                                              aCCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGTCGACGGTAACGtt                                                                                                    >  2:1189231/1‑150 (MQ=255)
                                              aCCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGtt                                                                                                    <  1:1135423/150‑1 (MQ=255)
                                                   gAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCa                                                                                               <  1:3838542/150‑1 (MQ=255)
                                                      aCATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCAttt                                                                                            <  1:3964599/150‑1 (MQ=255)
                                                      aCATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCAttt                                                                                            <  2:3985576/150‑1 (MQ=255)
                                                      aCATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCAttt                                                                                            <  1:2170673/150‑1 (MQ=255)
                                                       cATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTg                                                                                           <  1:2896689/150‑1 (MQ=255)
                                                       cATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGGTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAAATCAAGCATCGGTAATCCGTAACCACGCTCAATAAATGTTTGCAGGCGACGGTAACGTTCAGCATTTg                                                                                           >  1:3620347/1‑150 (MQ=255)
                                                          gCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCg                                                                                        <  1:4749921/150‑1 (MQ=255)
                                                            aaCGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGa                                                                                      >  1:2015518/1‑150 (MQ=255)
                                                            aaCGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGa                                                                                      >  1:2365429/1‑150 (MQ=255)
                                                             aCGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGAt                                                                                     <  1:2282515/150‑1 (MQ=255)
                                                             aCGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGAt                                                                                     <  2:48912/150‑1 (MQ=255)
                                                              cGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATc                                                                                    <  2:4940027/150‑1 (MQ=255)
                                                              cGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATc                                                                                    <  1:852455/150‑1 (MQ=255)
                                                              cGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATc                                                                                    >  1:3067862/1‑150 (MQ=255)
                                                              cGCATGATACATCTCAATAATGTAGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATc                                                                                    <  2:1827767/150‑1 (MQ=255)
                                                              cGCATGATACATCTACATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATc                                                                                    <  2:167951/150‑1 (MQ=255)
                                                                cATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGa                                                                                  <  1:7385/150‑1 (MQ=255)
                                                                  tGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAAACCGGAACCACGCTCAATAATTTTTTGCAGGCGACGGTAACCTTCAGCATTTGCCGGATAGAGc                                                                                >  1:3218717/1‑150 (MQ=255)
                                                                   gATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCa                                                                               >  2:3205398/1‑150 (MQ=255)
                                                                    aTACATCTCCATACTGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCAt                                                                              <  2:5077056/150‑1 (MQ=255)
                                                                    aTACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCAt                                                                              >  2:4742612/1‑150 (MQ=255)
                                                                    aTACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCAt                                                                              >  1:5175637/1‑150 (MQ=255)
                                                                     tACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGATCAGCATTAGCCGGATCGAGCATa                                                                             >  1:5308916/1‑150 (MQ=255)
                                                                      aCATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAg                                                                            <  1:3398770/150‑1 (MQ=255)
                                                                       cATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGt                                                                           <  1:1433574/150‑1 (MQ=255)
                                                                       cATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGt                                                                           <  2:1942643/150‑1 (MQ=255)
                                                                        aTCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGtc                                                                          >  2:146093/1‑150 (MQ=255)
                                                                        aTCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGtc                                                                          >  1:2594623/1‑150 (MQ=255)
                                                                            ccATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGtcatca                                                                      <  1:2096364/150‑1 (MQ=255)
                                                                                   gTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCTGATCGAGCATAGTCATCATCTTGTa                                                               >  1:2556934/1‑150 (MQ=255)
                                                                                      gatgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACTCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTg                                                            >  2:2642496/1‑150 (MQ=255)
                                                                                         gatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGAtt                                                         <  2:3742107/150‑1 (MQ=255)
                                                                                          atAGTGCGGCCGGTTTCTTCTTTCAGCTCGCCAATCTCGCGATCCAATTCACGCATCGGTATTCCGTAACCACGCTCTTTAATTGTTTGCAGGCGACGGTTACGTTCAGCATTTGCCGGATCGAGCATTGTCATCATCTTGTACTGATTa                                                        <  2:2127598/150‑1 (MQ=255)
                                                                                          atAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTa                                                        >  2:2839032/1‑150 (MQ=255)
                                                                                           tAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAg                                                       >  1:4123674/1‑150 (MQ=255)
                                                                                            aGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGa                                                      <  2:1297044/150‑1 (MQ=255)
                                                                                            aGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGa                                                      <  2:4497247/150‑1 (MQ=255)
                                                                                                 ggCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACaaaa                                                 <  1:1693493/150‑1 (MQ=255)
                                                                                                 ggCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATACAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACaaaa                                                 >  2:2719170/1‑150 (MQ=255)
                                                                                                   cAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATa                                               >  2:2626004/1‑150 (MQ=255)
                                                                                                     ggTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAg                                             <  1:3359200/150‑1 (MQ=255)
                                                                                                      gTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTATCCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGa                                            >  1:26751/1‑150 (MQ=255)
                                                                                                       tttCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGAc                                           <  1:1965211/150‑1 (MQ=255)
                                                                                                         tcttctTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGt                                         <  1:4568891/150‑1 (MQ=255)
                                                                                                          cttcttTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGtt                                        >  2:5030264/1‑150 (MQ=255)
                                                                                                          cttcttTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGtt                                        >  2:2449606/1‑150 (MQ=255)
                                                                                                           ttcttTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTg                                       <  1:589776/150‑1 (MQ=255)
                                                                                                            tcttTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTgc                                      <  1:2987450/150‑1 (MQ=255)
                                                                                                             cttTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTgcg                                     >  2:4576847/1‑150 (MQ=255)
                                                                                                                  aGCTCGCCAAACTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTg                                >  2:2938246/1‑150 (MQ=255)
                                                                                                                          aaaCTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTc                        >  1:4376605/1‑150 (MQ=255)
                                                                                                                          aaaCTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTc                        >  2:5271252/1‑150 (MQ=255)
                                                                                                                           aaCTCGCGATCCTAGTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTcc                       <  1:2456984/150‑1 (MQ=255)
                                                                                                                           aaCTCGCGATCCAAGTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTcc                       <  2:1033757/150‑1 (MQ=255)
                                                                                                                             cTCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAt                     >  1:1931452/1‑150 (MQ=255)
                                                                                                                              tCGCGATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAtt                    <  1:1603405/150‑1 (MQ=255)
                                                                                                                                gcgATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTGTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGTCGTTGCGCGTTGGTCATTTCCAttgt                  <  2:643664/150‑1 (MQ=255)
                                                                                                                                  gATCCAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAttgttg                >  2:1448657/1‑150 (MQ=255)
                                                                                                                                      cAATTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTc            >  1:1096256/1‑150 (MQ=255)
                                                                                                                                        aTTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCt          >  2:4592465/1‑150 (MQ=255)
                                                                                                                                        aTTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCt          >  2:2252881/1‑150 (MQ=255)
                                                                                                                                        aTTCACGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCt          <  1:4669628/150‑1 (MQ=255)
                                                                                                                                            aCGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTAt      >  1:4516870/1‑150 (MQ=255)
                                                                                                                                             cGCATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATc     <  2:4055322/150‑1 (MQ=255)
                                                                                                                                               cATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAGTGTTGACTCCTGTATCAc   >  1:5115112/1‑150 (MQ=255)
                                                                                                                                               cATCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCAATTGTTGACTCCTGTATCAc   >  1:3604486/1‑150 (MQ=255)
                                                                                                                                                aTCGGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCAct  >  1:4914700/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAGGTGACACGACGTTCATCGATGGATTGCTGGTCCTGCAAATTAGACCAGGAAACATGCAACGCATGATACATCTCCATAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACT  >  CP009273/2405799‑2406092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑