BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,456,705	C→A	100%	S72Y (TCC→TAC)	yfdF →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,456,705	0	C	A	100.0%	990.6 / NA	298	S72Y (TCC→TAC)	yfdF	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (162/136); total (162/136)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 298 total aligned reads displayed.

ATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGAGAAGA  >  CP009273/2456558‑2456851

atgatgAAGTTAATGGATTAGTACAATAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACt                                                                                                                                                  <  1:1016084/150‑1 (MQ=255)

atgatgAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACt                                                                                                                                                  <  2:3010342/150‑1 (MQ=255)

 tgatgaAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTc                                                                                                                                                 >  2:1645275/1‑150 (MQ=255)

  gatgaAGTTAATTGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCa                                                                                                                                                <  2:3752072/150‑1 (MQ=255)

  gatgaAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCa                                                                                                                                                <  2:3998407/150‑1 (MQ=255)

  gaagaAGTTAATGGATTAGAACAAGAGTTCAAAAACCTTATTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATAACTCa                                                                                                                                                >  2:1882717/4‑150 (MQ=255)

   atgaAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAg                                                                                                                                               <  2:4397735/150‑1 (MQ=255)

   atgaAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAg                                                                                                                                               >  2:478787/1‑150 (MQ=255)

      aaGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTa                                                                                                                                            <  2:1503926/150‑1 (MQ=255)

      aaGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTa                                                                                                                                            >  1:2420924/1‑150 (MQ=255)

       aGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTAc                                                                                                                                           >  1:1417095/1‑150 (MQ=255)

        gTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACt                                                                                                                                          <  2:671612/150‑1 (MQ=255)

        gTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACt                                                                                                                                          >  1:1660713/1‑150 (MQ=255)

           aaTGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGTTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTtat                                                                                                                                       <  2:4469009/150‑1 (MQ=255)

           aaTGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTtat                                                                                                                                       >  1:2486469/1‑150 (MQ=255)

             tgtaTTAGTACAAGAGTTCAAAAACCTTTTTAATGTTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatat                                                                                                                                     <  1:81216/147‑1 (MQ=255)

             tGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatat                                                                                                                                     <  2:3340459/150‑1 (MQ=255)

              ggATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTACACGACGATCCTAATAGATTTAATATTAATAATTACTCAGTTACTTatatt                                                                                                                                    >  2:2765817/1‑150 (MQ=255)

              ggATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatatt                                                                                                                                    >  1:4496396/1‑150 (MQ=255)

                 ttAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattgat                                                                                                                                 <  2:3063451/150‑1 (MQ=255)

                 ttAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattgat                                                                                                                                 <  2:2456306/150‑1 (MQ=255)

                  tAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAATGAATAAGCACATGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattgata                                                                                                                                <  2:3372889/150‑1 (MQ=255)

                  tAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattgata                                                                                                                                <  2:2146038/150‑1 (MQ=255)

                  tAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattgata                                                                                                                                <  2:646716/150‑1 (MQ=255)

                  tAGTACAAGAGTTAAAAAACATTTTTATTGGTAAGTAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattgata                                                                                                                                <  2:4915735/150‑1 (MQ=255)

                   aGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattgatat                                                                                                                               <  1:5151536/150‑1 (MQ=255)

                    gTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattgatatt                                                                                                                              <  1:1212470/150‑1 (MQ=255)

                     tACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattgatattg                                                                                                                             >  1:3789366/1‑150 (MQ=255)

                     tACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTatattgatattg                                                                                                                             <  1:5098894/150‑1 (MQ=255)

                        aaGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACt                                                                                                                          <  2:5313710/150‑1 (MQ=255)

                        aaGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGAATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACt                                                                                                                          >  2:4723411/1‑150 (MQ=255)

                         agagTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTc                                                                                                                         >  1:5269581/1‑150 (MQ=255)

                          gagTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTcc                                                                                                                        >  1:3599603/1‑150 (MQ=255)

                             ttCAAAAACATTTTTAATGTTAATGAAGTAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAAt                                                                                                                     <  1:2839499/150‑1 (MQ=255)

                             tacAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACTCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAAt                                                                                                                     <  2:3509303/148‑1 (MQ=255)

                                  aaaCCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATATATTTAATATTAATAATTACTCAGTTACTTATATAGATATTGACTCCAATGATaa                                                                                                                >  1:4839115/1‑149 (MQ=255)

                                  aaaCCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATAc                                                                                                                <  1:3065303/150‑1 (MQ=255)

                                     ccTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGa                                                                                                             >  1:1535969/1‑150 (MQ=255)

                                      cTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGAc                                                                                                            <  2:111399/150‑1 (MQ=255)

                                       tttttAATGGTATGGAAGGAATACGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGAcc                                                                                                           <  2:1953540/150‑1 (MQ=255)

                                         tttAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCat                                                                                                         <  1:3276893/150‑1 (MQ=255)

                                            aaTGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATAtt                                                                                                      >  1:5718/1‑150 (MQ=255)

                                                 tAATGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTAt                                                                                                 <  2:947146/150‑1 (MQ=255)

                                                 tAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTAt                                                                                                 >  1:2755440/1‑150 (MQ=255)

                                                  aaggaaggAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTAtt                                                                                                <  1:216322/150‑1 (MQ=255)

                                                  aaggaaggAATAAGCACCTGTAATAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTAATATTGATATTGACTCCAATGATACAGACCATATTACTAtt                                                                                                >  1:3329258/1‑150 (MQ=255)

                                                   agtaaggaaTAAGCACCTGTATAAAAAATCTACTTGAGATTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTg                                                                                               <  1:1846362/147‑1 (MQ=255)

                                                           aTAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGAc                                                                                       <  2:612748/150‑1 (MQ=255)

                                                            tAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACa                                                                                      >  2:5242601/1‑150 (MQ=255)

                                                             aaGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCTACaa                                                                                     >  2:1313660/1‑150 (MQ=255)

                                                               gCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAAcc                                                                                   >  1:4691641/1‑150 (MQ=255)

                                                                 tacTGTATTAAACATCTACTTGAGCTTTTAATAAACGCCATAGGTGTAAACGACGATCCTTATAGATTTATTATTAATAATTACTCTGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCaa                                                                                 <  2:3585151/148‑1 (MQ=255)

                                                                  ccTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAg                                                                                <  2:4158635/150‑1 (MQ=255)

                                                                        ttAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCaa                                                                          >  2:952651/1‑150 (MQ=255)

                                                                         tAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAAt                                                                         <  2:4680421/150‑1 (MQ=255)

                                                                         tAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAAt                                                                         <  2:2667407/150‑1 (MQ=255)

                                                                         tAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAAt                                                                         <  1:4150349/150‑1 (MQ=255)

                                                                             cATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGaa                                                                     <  2:1169432/150‑1 (MQ=255)

                                                                                   cTTGAGATTATAAAAAACGCCATACGATTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCt                                                               <  1:4021512/150‑1 (MQ=255)

                                                                                    ttGATCTTATAAAAAACGCCATACTAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTg                                                              <  2:2333434/150‑1 (MQ=255)

                                                                                      gAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCg                                                            <  1:4374827/150‑1 (MQ=255)

                                                                                           tataAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACta                                                       >  1:1936086/1‑150 (MQ=255)

                                                                                                aaaaCGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAg                                                  <  1:1116852/150‑1 (MQ=255)

                                                                                                aaaaCGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAg                                                  <  1:2675576/150‑1 (MQ=255)

                                                                                                aaaaCGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAg                                                  <  2:2602084/150‑1 (MQ=255)

                                                                                                aaaaCGCAATACGAGTAAACGACGATCCTAATAGATTTAATATTAAAAATTACTCAGTTACTTATATTGATATTGACTCCAAATATACAGACAATATTACTATTTTTATCGACAAACAACAACCAATAGAATTACCTGCGAACTATAAAg                                                  >  2:4078564/1‑150 (MQ=255)

                                                                                                      ccATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAg                                            <  2:695071/150‑1 (MQ=255)

                                                                                                      ccATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAg                                            >  2:1003346/1‑150 (MQ=255)

                                                                                                      ccATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAg                                            <  1:2630892/150‑1 (MQ=255)

                                                                                                       cATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGa                                           <  2:1660713/150‑1 (MQ=255)

                                                                                                         tACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAAc                                         <  1:659338/150‑1 (MQ=255)

                                                                                                         tACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAAc                                         <  2:4987672/150‑1 (MQ=255)

                                                                                                          aCGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACt                                        <  1:1484386/150‑1 (MQ=255)

                                                                                                          aCGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACt                                        <  2:326894/150‑1 (MQ=255)

                                                                                                            gAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCg                                      <  1:4256154/150‑1 (MQ=255)

                                                                                                            gAGTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCg                                      >  2:2958231/1‑150 (MQ=255)

                                                                                                              gTAAACGACGATCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTc                                    >  1:3168672/1‑150 (MQ=255)

                                                                                                                    gacgaTCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACt                              >  2:1464579/1‑150 (MQ=255)

                                                                                                                    gacgaTCATTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACt                              >  2:868225/1‑150 (MQ=255)

                                                                                                                      cgaTCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTAt                            >  1:298837/1‑150 (MQ=255)

                                                                                                                         tCCTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCAt                         <  2:3825135/150‑1 (MQ=255)

                                                                                                                           cTTATAGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTa                       >  1:4328256/1‑150 (MQ=255)

                                                                                                                             tataGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAAt                     >  1:4575456/1‑150 (MQ=255)

                                                                                                                              ataGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATg                    <  2:2534146/150‑1 (MQ=255)

                                                                                                                              ataGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATg                    >  1:743225/1‑150 (MQ=255)

                                                                                                                              ataGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATg                    <  2:3611590/150‑1 (MQ=255)

                                                                                                                                aGATTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGac                  >  2:1393433/1‑150 (MQ=255)

                                                                                                                                  aTTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGacaa                >  1:1033990/1‑150 (MQ=255)

                                                                                                                                  aTTTAATATTAATAATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCACTAATGacaa                >  1:5049238/1‑150 (MQ=255)

                                                                                                                                    ttaatattaataATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGacaaca              >  2:2196103/1‑150 (MQ=255)

                                                                                                                                       atattaataATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAg           >  2:3930538/1‑150 (MQ=255)

                                                                                                                                         attaataATTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGtt         >  2:3706291/1‑150 (MQ=255)

                                                                                                                                           taataaTTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTga       >  2:535225/1‑150 (MQ=255)

                                                                                                                                           taataaTTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTga       >  1:1733882/1‑150 (MQ=255)

                                                                                                                                            aataatTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTgag      >  1:3402138/1‑150 (MQ=255)

                                                                                                                                             ataatTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTgaga     >  1:928127/1‑150 (MQ=255)

                                                                                                                                             ataatTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTgaga     >  1:2435950/1‑150 (MQ=255)

                                                                                                                                               aatTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGagaag   >  1:3738494/1‑150 (MQ=255)

                                                                                                                                                atTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGagaaga  >  2:2232425/1‑150 (MQ=255)

                                                                                                                                                atTACTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGagaaga  >  1:2273413/1‑150 (MQ=255)

ATGATGAAGTTAATGGATTAGTACAAGAGTTCAAAAACCTTTTTAATGGTAAGGAAGGAATAAGCACCTGTATTAAACATCTACTTGAGCTTATAAAAAACGCCATACGAGTAAACGACGATCCTTATAGATTTAATATTAATAATTCCTCAGTTACTTATATTGATATTGACTCCAATGATACAGACCATATTACTATTGGTATCGACAACCAAGAACCAATAGAATTACCTGCGAACTATAAAGACAAAGAACTCGTCCGTACTATCATTAATGACAACATAGTTGAGAAGA  >  CP009273/2456558‑2456851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`