Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,476,524 T→A 100% N99Y (AAT→TAT)  emrK ← EmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,476,5240TA99.1% 1115.6 / ‑1.4 349N99Y (AAT→TAT) emrKEmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component
Reads supporting (aligned to +/- strand):  ref base T (1/4);  new base A (190/154);  total (191/158)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 364 total aligned reads displayed.

TAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGA  >  CP009273/2476377‑2476670
                                                                                                                                                   |                                                                                                                                                  
tAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTAtatt                                                                                                                                                  <  1:3883481/150‑1 (MQ=255)
 aaCCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTAtattt                                                                                                                                                 >  1:1570274/1‑150 (MQ=255)
  aCCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTa                                                                                                                                                >  1:294625/1‑150 (MQ=255)
  aCCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTa                                                                                                                                                >  2:2251288/1‑150 (MQ=255)
    ccccTGCTTCGCTAACGGCACTCGACGGTTATAATATTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGCTTATATTTAGc                                                                                                                                              <  1:4905058/150‑1 (MQ=255)
     cccTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCt                                                                                                                                             >  2:4423547/1‑150 (MQ=255)
     cccTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCt                                                                                                                                             <  2:1617988/150‑1 (MQ=255)
          cTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTAtt                                                                                                                                        <  1:648989/150‑1 (MQ=255)
          cTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATAGTTGCCAGAGTATATTTAGCTTTAtt                                                                                                                                        >  2:5231403/1‑150 (MQ=255)
           ttCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTg                                                                                                                                       <  1:3255188/150‑1 (MQ=255)
           ttCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTg                                                                                                                                       >  2:3433831/1‑150 (MQ=255)
           ttCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTg                                                                                                                                       <  1:5264497/150‑1 (MQ=255)
                 aaCGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCg                                                                                                                                 <  2:3178261/150‑1 (MQ=255)
                   cGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGAt                                                                                                                               >  2:83804/1‑150 (MQ=255)
                     gCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAg                                                                                                                             <  1:2026964/150‑1 (MQ=255)
                        cTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTgg                                                                                                                          >  1:2596477/1‑150 (MQ=255)
                         tCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGc                                                                                                                         <  2:5012203/150‑1 (MQ=255)
                                ttATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGtt                                                                                                                  <  1:4989262/150‑1 (MQ=255)
                                  ataATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGtttt                                                                                                                <  1:196651/150‑1 (MQ=255)
                                          ctAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTg                                                                                                        >  2:4811758/1‑150 (MQ=255)
                                                aTTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTa                                                                                                  <  2:3646752/150‑1 (MQ=255)
                                                aTTGTTGATACNGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTa                                                                                                  >  1:2764050/1‑150 (MQ=255)
                                                   gttgATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTaaaa                                                                                               >  1:2186472/1‑150 (MQ=255)
                                                   gttgATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTaaaa                                                                                               <  2:1227689/150‑1 (MQ=255)
                                                     tgATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATg                                                                                             >  2:3862189/1‑150 (MQ=255)
                                                     tgATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATg                                                                                             <  1:3974948/150‑1 (MQ=255)
                                                     tgATACTGAATACGTGCTGAAGCGACTACGGCACTGTATTGTTTATCCTGTAAGTATAGTTAATACGTTTGCCGAACAATATTTGCCAGAGAATATTAAGCTTAATAGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATg                                                                                             >  1:4699219/1‑150 (MQ=255)
                                                        tACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCa                                                                                          >  1:4583613/1‑150 (MQ=255)
                                                        tACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCa                                                                                          <  1:238661/150‑1 (MQ=255)
                                                         aCTGAATACGTGCTGAAGCGACTTCGGCTCTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTGGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCATTGAAACTAAAATGTCAc                                                                                         <  2:2519035/150‑1 (MQ=255)
                                                         aCTGAAGACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCAc                                                                                         <  2:270828/150‑1 (MQ=255)
                                                            gAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCtt                                                                                      >  1:4127650/1‑150 (MQ=255)
                                                            gAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCtt                                                                                      <  2:369362/150‑1 (MQ=255)
                                                             aaTACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTg                                                                                     <  1:3187137/150‑1 (MQ=255)
                                                                aCGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCg                                                                                  >  1:701264/1‑150 (MQ=255)
                                                                  gTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGaa                                                                                >  1:2856868/1‑150 (MQ=255)
                                                                      tGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTa                                                                            <  2:1761632/150‑1 (MQ=255)
                                                                       gAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAg                                                                           <  2:3914716/150‑1 (MQ=255)
                                                                        aaGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATGTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTAGTCGAACGTAGt                                                                          >  2:4280403/1‑150 (MQ=255)
                                                                           cGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCg                                                                       <  1:2643448/150‑1 (MQ=255)
                                                                           cGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCg                                                                       <  1:3689690/150‑1 (MQ=255)
                                                                           cGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCg                                                                       <  2:431497/150‑1 (MQ=255)
                                                                           cGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCg                                                                       >  1:3358589/1‑150 (MQ=255)
                                                                            gACTTCGTCACTGTAATGTTTATCAGGTAAGTATATTTTATTCGTTTTCCGAACAATATTTTCCAGAAAATATTAAGCTTAATTGAGTGCGATAGTGGACTCAGTTTTATCCAGTGAAACTAAAATGTCACCTATTCGAACTTAGTTCGt                                                                      >  2:1358535/1‑150 (MQ=255)
                                                                             aCTTCGGCACTGTATTGATTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTa                                                                     <  1:2235628/150‑1 (MQ=255)
                                                                              cTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTAt                                                                    >  1:1754277/1‑150 (MQ=255)
                                                                              cTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTAt                                                                    >  2:2177757/1‑150 (MQ=255)
                                                                              cTTCGGCACTGGATTGTTTCTCCTGGAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTAt                                                                    <  2:4377568/150‑1 (MQ=255)
                                                                                 cGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCtt                                                                 >  1:1446335/1‑150 (MQ=255)
                                                                                  ggCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCttt                                                                >  2:4679348/1‑150 (MQ=255)
                                                                                  ggCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCttt                                                                <  2:4206893/150‑1 (MQ=255)
                                                                                   gCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTa                                                               <  1:5211003/150‑1 (MQ=255)
                                                                                    cACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTAt                                                              <  2:684692/150‑1 (MQ=255)
                                                                                        gTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGAtt                                                          >  2:4289216/1‑150 (MQ=255)
                                                                                        gTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGAtt                                                          >  1:1340791/1‑150 (MQ=255)
                                                                                        gTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGAtt                                                          <  2:2431862/150‑1 (MQ=255)
                                                                                        gTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGAtt                                                          >  1:978464/1‑150 (MQ=255)
                                                                                            tGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACg                                                      <  1:1898032/150‑1 (MQ=255)
                                                                                             gTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGa                                                     >  1:4276964/1‑150 (MQ=255)
                                                                                             gTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGa                                                     >  1:351230/1‑150 (MQ=255)
                                                                                              tttATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGAc                                                    >  2:1836765/1‑150 (MQ=255)
                                                                                                 aTCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGt                                                 >  2:3746438/1‑150 (MQ=255)
                                                                                                 aTCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGt                                                 >  1:3626175/1‑150 (MQ=255)
                                                                                                   ccTGTAAGAATAGTTAATTCGTTTGCCGAACAATATAAGCCACATAAAATTTAGCTATATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCATGTCGAACGTAGTTCGTATCTTTATGATAAACGACAgaga                                               >  2:3230631/1‑150 (MQ=255)
                                                                                                    cTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGac                                              >  1:5106101/1‑150 (MQ=255)
                                                                                                    cTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGac                                              >  1:639229/1‑150 (MQ=255)
                                                                                                    cTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGac                                              >  1:3672761/1‑150 (MQ=255)
                                                                                                      gTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGacac                                            >  2:2107450/1‑150 (MQ=255)
                                                                                                       tAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACt                                           >  1:2510770/1‑150 (MQ=255)
                                                                                                       tAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACt                                           <  2:2945047/150‑1 (MQ=255)
                                                                                                         aGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTAc                                         >  1:3068117/1‑150 (MQ=255)
                                                                                                           tataGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCt                                       >  2:4469219/1‑150 (MQ=255)
                                                                                                            ataGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTg                                      >  1:3927086/1‑150 (MQ=255)
                                                                                                              aGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTgag                                    <  2:281300/150‑1 (MQ=255)
                                                                                                               gTTTATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTgaga                                   >  1:1290585/1‑150 (MQ=255)
                                                                                                                  tATTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACtt                                >  1:4407701/1‑150 (MQ=255)
                                                                                                                   aTTCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTtg                               >  2:1894415/1‑150 (MQ=255)
                                                                                                                    ttCGTTTGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTtgt                              <  1:3784091/150‑1 (MQ=255)
                                                                                                                         ttGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGACATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGa                         >  1:794799/1‑150 (MQ=255)
                                                                                                                          tGCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGaa                        >  1:288284/1‑150 (MQ=255)
                                                                                                                           gCCGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGaaa                       >  1:3464019/1‑150 (MQ=255)
                                                                                                                             cGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAAtt                     >  1:1784422/1‑150 (MQ=255)
                                                                                                                             cGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAAtt                     <  2:1467368/150‑1 (MQ=255)
                                                                                                                             cGAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAAtt                     <  2:4538455/150‑1 (MQ=255)
                                                                                                                              gAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTg                    >  1:1522302/1‑150 (MQ=255)
                                                                                                                              gAACAATATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTg                    <  2:727134/150‑1 (MQ=255)
                                                                                                                                  aaTATTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATc                >  2:537388/1‑150 (MQ=255)
                                                                                                                                   atatTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCATTTTTATCCAGTTAAACTAAATTGTCACCTTGTCGAACGTAGTTCGTATCTTTTTGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCt               <  1:4551712/150‑1 (MQ=255)
                                                                                                                                   atatTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCt               <  2:617041/150‑1 (MQ=255)
                                                                                                                                   atatTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCt               >  1:1854076/1‑150 (MQ=255)
                                                                                                                                   atatTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCt               >  2:460393/1‑150 (MQ=255)
                                                                                                                                    tatTTGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTg              >  2:3530846/1‑150 (MQ=255)
                                                                                                                                        tGCCAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCAtt          <  2:1570274/150‑1 (MQ=255)
                                                                                                                                           cAGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTccc       <  2:860504/150‑1 (MQ=255)
                                                                                                                                            aGATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTcccc      >  2:4077893/1‑150 (MQ=255)
                                                                                                                                             gATTATATTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCg     >  2:3104608/1‑150 (MQ=255)
                                                                                                                                              attataTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATTATTAACGACAGTGACACTACCTGAGACATGTGCAGAAATTGGATCTGCATTCCCCGt    >  1:1062726/1‑150 (MQ=255)
                                                                                                                                              attataTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGt    >  2:504181/1‑150 (MQ=255)
                                                                                                                                              attataTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGt    >  1:4840565/1‑150 (MQ=255)
                                                                                                                                               ttataTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAAAGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTg   >  2:2301976/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TAACCCCCTGCTTCGCTAACGGCACTCGACGGTTATAATCTTCTAAAGATTGTTGATACTGAATACGTGCTGAAGCGACTTCGGCACTGTATTGTTTATCCTGTAAGTATAGTTTATTCGTTTGCCGAACAATATTTGCCAGATTATTTTTAGCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGA  >  CP009273/2476377‑2476670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑