BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,499,180	G→T	22.6%	T311K (ACG→AAG)	ypdF ←	Xaa‑Pro aminopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,499,180	0	G	T	22.6%	716.5 / 142.8	282	T311K (ACG→AAG)	ypdF	Xaa‑Pro aminopeptidase
Reads supporting (aligned to +/- strand): ref base G (89/129); new base T (31/33); total (120/162)
Fisher's exact test for biased strand distribution p-value = 3.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 294 total aligned reads displayed.

CCTCTCCCGTGAGCAACACTGTTTTCGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGG  >  CP009273/2499031‑2499328

 ctctCCCGTGAGCAACACTGTTTTCGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGcgt                                                                                                                                                     <  2:1109090/150‑1 (MQ=255)

  tctcCCGTGAGCAACACTGTTTTCGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGcgtc                                                                                                                                                    <  1:5014519/150‑1 (MQ=255)

  tctcCCGTGAGCAACACTGTTTTCGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGcgtc                                                                                                                                                    <  1:5095367/150‑1 (MQ=255)

   ctcCCGTGAGCAACACTGTTTTCGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGcgtcg                                                                                                                                                   >  1:4882949/1‑150 (MQ=255)

   ctcCCGTGAGCAACACTGTTTTCGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGcgtcg                                                                                                                                                   <  1:3144831/150‑1 (MQ=255)

       cGTGAGCAACACTGTTTTCGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCgtggt                                                                                                                                               >  1:535246/1‑150 (MQ=255)

            gCAACACTGTTTTCGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTccc                                                                                                                                          >  2:5168248/1‑150 (MQ=255)

              aaCACTGTTTTCGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCcgc                                                                                                                                        <  2:3581875/150‑1 (MQ=255)

              aaCACTGTTTTCGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCcgc                                                                                                                                        >  1:3643652/1‑150 (MQ=255)

              aaCACTGTTTTCGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCcgc                                                                                                                                        <  1:296271/150‑1 (MQ=255)

              aaCACTGTTTTCGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCcgc                                                                                                                                        >  1:1785987/1‑150 (MQ=255)

                  cTGTTTTCGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAACTCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTg                                                                                                                                    <  2:2456285/150‑1 (MQ=255)

                  cTGTTTTCGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTg                                                                                                                                    >  2:1588532/1‑150 (MQ=255)

                  cTGTTTTCGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTg                                                                                                                                    >  2:2735018/1‑150 (MQ=255)

                        tCGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAAc                                                                                                                              <  2:4110115/150‑1 (MQ=255)

                         cGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAAcg                                                                                                                             <  1:2830182/150‑1 (MQ=255)

                            cATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCgg                                                                                                                          <  1:428593/150‑1 (MQ=255)

                              tGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGAt                                                                                                                        <  1:1955352/150‑1 (MQ=255)

                                gCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCt                                                                                                                      <  2:3172336/150‑1 (MQ=255)

                                gCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCt                                                                                                                      <  2:4186888/150‑1 (MQ=255)

                                 cGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCtt                                                                                                                     <  1:3060029/150‑1 (MQ=255)

                                 cGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCtt                                                                                                                     <  2:1998445/150‑1 (MQ=255)

                                 cGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCtt                                                                                                                     <  2:4652437/150‑1 (MQ=255)

                                  gTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTc                                                                                                                    >  2:4061818/1‑150 (MQ=255)

                                  gTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTc                                                                                                                    >  2:1781559/1‑150 (MQ=255)

                                  gTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTc                                                                                                                    >  1:184038/1‑150 (MQ=255)

                                         aCTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACt                                                                                                             >  1:1476410/1‑150 (MQ=255)

                                         aCTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACt                                                                                                             <  1:874913/150‑1 (MQ=255)

                                         aCTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCGGCATGAACt                                                                                                             >  1:4943774/1‑150 (MQ=255)

                                          cTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACtt                                                                                                            <  2:777821/150‑1 (MQ=255)

                                          cTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGGCCCGCGGTGAAAAACGCGGATCTTCATGAACtt                                                                                                            >  2:2604150/1‑150 (MQ=255)

                                            tCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCa                                                                                                          <  1:2708836/150‑1 (MQ=255)

                                              tGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAAt                                                                                                        <  2:5055975/150‑1 (MQ=255)

                                                 gcCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGcc                                                                                                     >  1:1318492/1‑150 (MQ=255)

                                                   cTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGa                                                                                                   <  2:4764482/150‑1 (MQ=255)

                                                     tGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATa                                                                                                 <  2:1351093/150‑1 (MQ=255)

                                                          ggTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTg                                                                                            <  1:1092650/150‑1 (MQ=255)

                                                            tAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGAc                                                                                          >  1:3917949/1‑150 (MQ=255)

                                                            tAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGAc                                                                                          >  1:1614437/1‑150 (MQ=255)

                                                             aaCCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCTTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGAcc                                                                                         <  1:4071773/150‑1 (MQ=255)

                                                             aaCCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCTTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGAcc                                                                                         <  1:3705004/150‑1 (MQ=255)

                                                             aaCCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCTTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGAcc                                                                                         <  2:1473976/150‑1 (MQ=255)

                                                                  gCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGgtgt                                                                                    <  1:1223583/150‑1 (MQ=255)

                                                                  gCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAAGGCCGATAGCGTGACCGgtgt                                                                                    >  1:2287647/1‑150 (MQ=255)

                                                                   cacaACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGtt                                                                                   >  1:898137/1‑150 (MQ=255)

                                                                     caacaTCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTAt                                                                                 <  2:4968935/150‑1 (MQ=255)

                                                                     caacaTCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTAt                                                                                 >  2:1930514/1‑150 (MQ=255)

                                                                      aacaTCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAACTCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATg                                                                                <  2:688845/150‑1 (MQ=255)

                                                                      aacaTCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATg                                                                                >  2:2051483/1‑150 (MQ=255)

                                                                      aacaTCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATg                                                                                <  1:2123394/150‑1 (MQ=255)

                                                                      aacaTCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATg                                                                                >  1:421324/1‑150 (MQ=255)

                                                                         aTCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGTcc                                                                             >  2:3996192/1‑150 (MQ=255)

                                                                            ttCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCTTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGaa                                                                          <  2:4299787/150‑1 (MQ=255)

                                                                            ttCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGaa                                                                          >  1:3241309/1‑150 (MQ=255)

                                                                              cGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCTTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAAt                                                                        <  1:2246174/150‑1 (MQ=255)

                                                                               gATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATa                                                                       <  2:901755/150‑1 (MQ=255)

                                                                                aTGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCTTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAg                                                                      >  2:4170588/1‑150 (MQ=255)

                                                                                aTGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAg                                                                      <  2:2188370/150‑1 (MQ=255)

                                                                                  gcgcACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTc                                                                    <  1:1344467/150‑1 (MQ=255)

                                                                                   cgcACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCg                                                                   <  1:5322808/150‑1 (MQ=255)

                                                                                    gcACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCTTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGc                                                                  <  2:1614201/150‑1 (MQ=255)

                                                                                     cACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGcc                                                                 <  1:1638848/150‑1 (MQ=255)

                                                                                     cACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGcc                                                                 >  1:2648535/1‑150 (MQ=255)

                                                                                      aCGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCa                                                                >  2:3660538/1‑150 (MQ=255)

                                                                                      aCGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCa                                                                >  2:3494469/1‑150 (MQ=255)

                                                                                       cGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCTTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCag                                                               >  1:4521858/1‑149 (MQ=255)

                                                                                       cGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCAt                                                               <  1:353455/150‑1 (MQ=255)

                                                                                       cGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCAt                                                               <  1:515288/150‑1 (MQ=255)

                                                                                        gCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATa                                                              <  2:365211/150‑1 (MQ=255)

                                                                                             cTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTg                                                         <  1:795416/150‑1 (MQ=255)

                                                                                              ttGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCTTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGc                                                        >  1:2439884/1‑150 (MQ=255)

                                                                                                  ccTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCt                                                    >  1:3395780/1‑150 (MQ=255)

                                                                                                   cTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCTTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTg                                                   >  2:2512084/1‑150 (MQ=255)

                                                                                                   cTGGCAAATAAATCACCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCTTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTg                                                   >  1:3609307/1‑150 (MQ=255)

                                                                                                       cAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAAt                                               <  2:3368956/150‑1 (MQ=255)

                                                                                                       cAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAAt                                               <  2:2330577/150‑1 (MQ=255)

                                                                                                        aaataaatCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATg                                              <  2:4268329/150‑1 (MQ=255)

                                                                                                            aaatCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGAccc                                          <  2:3860441/150‑1 (MQ=255)

                                                                                                             aatCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCTTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCg                                         <  2:1541870/150‑1 (MQ=255)

                                                                                                             aatCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCg                                         <  1:4639855/150‑1 (MQ=255)

                                                                                                             aatCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCg                                         <  1:3931398/150‑1 (MQ=255)

                                                                                                                ccccGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGgcg                                      >  2:3937650/1‑150 (MQ=255)

                                                                                                                      cTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGc                                >  2:3014265/1‑150 (MQ=255)

                                                                                                                        ccACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGCAt                              >  1:5253568/1‑150 (MQ=255)

                                                                                                                           cGGTCAGTAACATGCCTGGCTGTAGCTTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGCATCGt                           >  1:4558575/1‑150 (MQ=255)

                                                                                                                            ggTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGTTCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGCATCGTc                          <  2:1287021/150‑1 (MQ=255)

                                                                                                                               cAGTAACATGCCTGGCTGTAGCTTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGCATCGTCAAc                       >  1:859656/1‑150 (MQ=255)

                                                                                                                                aGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGNCATCGTCAAcc                      <  1:2765198/150‑1 (MQ=255)

                                                                                                                                aGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGCATCGTCAAcc                      >  2:3723336/1‑150 (MQ=255)

                                                                                                                                aGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGCATCGTCAAcc                      <  1:622107/150‑1 (MQ=255)

                                                                                                                                 gTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGCATCGTCAACct                     >  1:1005486/1‑150 (MQ=255)

                                                                                                                                 gTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGCATCGTCAACct                     <  1:4338554/150‑1 (MQ=255)

                                                                                                                                 gTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCCCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCAGATAGCGTGACCGGTGTTATGACCGAAATAGAAGCCATAACCTGCTTCTGTAAAGACCCGGCGCGCGGCATCGTCAACct                     >  1:328850/1‑150 (MQ=255)

                                                                                                                                  tAACATGCCTGGCTGTAGCTTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGCATCGTCAACctg                    <  2:5270367/150‑1 (MQ=255)

                                                                                                                                   aaCATGCCTGGCTGTAGCTTCGTGGAGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACACGGCGCGCGGCATCGTCAACctgc                   >  2:2112462/1‑150 (MQ=255)

                                                                                                                                    aCATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGCATCGTCAACctgct                  <  1:5283614/150‑1 (MQ=255)

                                                                                                                                      aTGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGCATCGTCAACCTGCTgg                >  2:33283/1‑150 (MQ=255)

                                                                                                                                        gCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGCATCGTCAACCTGCTGGCa              <  2:1021625/150‑1 (MQ=255)

                                                                                                                                           tGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGCATCGTCAACCTGCTGGCAgcg           >  2:1663070/1‑150 (MQ=255)

                                                                                                                                               tGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTACAAGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCAAAACCTGCGTCTGTAATGACCCGGCGCGCGGCATCGTCAACCTGCTGGCAGCGCACg       >  1:2500733/1‑150 (MQ=255)

CCTCTCCCGTGAGCAACACTGTTTTCGGCATGGCGTAGAGCACTTCTGCGCCTTGCGGGGTAACCAGCACAACATCTTCGATGCGCACGCCCCCTTGCCCTGGCAAATAAATCCCCGGCTCCACGGTCAGTAACATGCCTGGCTGTAGCGTCGTGGTGTCCCGCGGTGAAAAACGCGGATCTTCATGAACTTCAATGCCGATAGCGTGACCGGTGTTATGACCGAAATAGTCGCCATAACCTGCTTCTGTAATGACCCGGCGCGCGGCATCGTCAACCTGCTGGCAGCGCACGCCGGG  >  CP009273/2499031‑2499328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`