Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,499,568 C→A 100% D182Y (GAC→TAC)  ypdF ← Xaa‑Pro aminopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,499,5680CA97.2% 895.5 / 13.2 291D182Y (GAC→TAC) ypdFXaa‑Pro aminopeptidase
Reads supporting (aligned to +/- strand):  ref base C (4/4);  new base A (132/151);  total (136/155)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 296 total aligned reads displayed.

ATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTTCTCC  >  CP009273/2499419‑2499713
                                                                                                                                                     |                                                                                                                                                 
aTTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTc                                                                                                                                                   >  2:1846880/1‑150 (MQ=255)
  tCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACATCGCACCGAAATCGAGAGTGACAAACTCGCACGATGCAACAATCTTGTCGCTGGCTTTGCCGAGCGGCATCTACCCAAGCCAGCCACTGGCGACAATGGTGTaaa                                                                                                                                                 >  1:3855169/1‑150 (MQ=255)
   cACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACACTGGTGTaaaa                                                                                                                                                >  2:1975113/1‑150 (MQ=255)
   cACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTaaaa                                                                                                                                                >  2:4634601/1‑150 (MQ=255)
       aGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAagag                                                                                                                                            <  1:4072573/150‑1 (MQ=255)
       aGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAagag                                                                                                                                            >  2:173031/1‑150 (MQ=255)
       aGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAagag                                                                                                                                            <  2:3282532/150‑1 (MQ=255)
         cAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGc                                                                                                                                          >  2:1636226/1‑150 (MQ=255)
           aGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCtt                                                                                                                                        >  2:3448279/1‑150 (MQ=255)
             gTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCtttt                                                                                                                                      <  2:58760/150‑1 (MQ=255)
              tGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCttttt                                                                                                                                     <  1:4786452/150‑1 (MQ=255)
                cgcgTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCt                                                                                                                                   <  1:1499614/150‑1 (MQ=255)
                   gTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTgcg                                                                                                                                >  2:51787/1‑150 (MQ=255)
                      atatCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGccc                                                                                                                             >  2:292474/1‑150 (MQ=255)
                        atCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCctg                                                                                                                           >  1:887067/1‑150 (MQ=255)
                        atCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCctg                                                                                                                           <  2:3507590/150‑1 (MQ=255)
                        atCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCctg                                                                                                                           <  1:3421861/150‑1 (MQ=255)
                           agagCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCctgctg                                                                                                                        >  1:3134892/1‑150 (MQ=255)
                           agagCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCctgctg                                                                                                                        >  1:3180393/1‑150 (MQ=255)
                             agCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGgc                                                                                                                      >  1:3945083/1‑150 (MQ=255)
                                  tAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATa                                                                                                                 <  1:3263736/150‑1 (MQ=255)
                                     cccTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAAGCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAAc                                                                                                              <  2:487640/150‑1 (MQ=255)
                                      ccTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCGGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAAcc                                                                                                             <  1:2170168/150‑1 (MQ=255)
                                         gATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACt                                                                                                          >  2:3335555/1‑150 (MQ=255)
                                          aTACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTc                                                                                                         <  2:3785780/150‑1 (MQ=255)
                                              aGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGt                                                                                                     <  1:1332499/150‑1 (MQ=255)
                                              aGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGt                                                                                                     >  1:3079651/1‑150 (MQ=255)
                                                cgcACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCCCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTc                                                                                                   >  1:2279561/1‑150 (MQ=255)
                                                 gcACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCa                                                                                                  >  1:3923642/1‑150 (MQ=255)
                                                     cGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCgg                                                                                              >  2:3315195/1‑150 (MQ=255)
                                                     cGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCgg                                                                                              <  2:2611083/150‑1 (MQ=255)
                                                      gAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACCATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACACCAGTTCAGCGGc                                                                                             >  2:4914398/1‑150 (MQ=255)
                                                      gAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGc                                                                                             >  2:1214219/1‑150 (MQ=255)
                                                        aaTCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTa                                                                                           >  2:2388019/1‑150 (MQ=255)
                                                         aTCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTAt                                                                                          <  2:3935693/150‑1 (MQ=255)
                                                                 tgtctaaCTCGCCCGGTGCAAAAATCTTGTCGCTGGCTTGGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACTATGGTGTAAAATGAGGCTTTTTCTGCTCCCGGCTGGGGCATAAACCTCTCCAGTTCAGCGGCTATCTCGcgct                                                                                  <  1:2425220/145‑1 (MQ=255)
                                                                  gACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgctc                                                                                 <  1:1050636/150‑1 (MQ=255)
                                                                  gACAAACTCGCCCGCTGAAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACATTGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgctc                                                                                 <  1:3585049/150‑1 (MQ=255)
                                                                   aCAACCTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCCGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgctcg                                                                                <  1:1098954/150‑1 (MQ=255)
                                                                     aaaCTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgctcgct                                                                              >  1:3537786/1‑150 (MQ=255)
                                                                     aaaCTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgctcgct                                                                              <  1:841275/150‑1 (MQ=255)
                                                                     aaaCTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGAGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGcgctcgct                                                                              <  1:597841/150‑1 (MQ=255)
                                                                      aaCTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCgctcgctc                                                                             <  1:4262841/150‑1 (MQ=255)
                                                                       aCTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCa                                                                            >  1:4860893/1‑150 (MQ=255)
                                                                        cTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCAt                                                                           <  2:2272500/150‑1 (MQ=255)
                                                                           gCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATccc                                                                        >  1:5078439/1‑150 (MQ=255)
                                                                            cccGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATcccc                                                                       <  2:44186/150‑1 (MQ=255)
                                                                            cccGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATcccc                                                                       <  2:4987215/150‑1 (MQ=255)
                                                                                 tGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTg                                                                  >  1:5168200/1‑150 (MQ=255)
                                                                                 tGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTg                                                                  <  1:1901105/150‑1 (MQ=255)
                                                                                 tGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGAATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTg                                                                  <  1:4557277/150‑1 (MQ=255)
                                                                                 tGCAACAATCTTGTCGCTGGCATTGCCGTGCGGCAGAGCCCCACGCCAGCCACAGTCGACAATGGTGTAAAAAGAGGCTTTTTCTGAGCCCTGCTGGCGCAAAAACCACTCCAGTTCATCGGCAATAACGCGCTCGCTCATCCACGCCTg                                                                  >  1:2910204/1‑150 (MQ=255)
                                                                                  gCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGa                                                                 <  1:5002731/150‑1 (MQ=255)
                                                                                  gCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGa                                                                 >  2:2486380/1‑150 (MQ=255)
                                                                                    aacaaTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAAt                                                               >  1:914232/1‑150 (MQ=255)
                                                                                      caaTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATaa                                                             >  2:1315455/1‑150 (MQ=255)
                                                                                      caaTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCCGAATaa                                                             <  1:3675547/150‑1 (MQ=255)
                                                                                      caaTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCGGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATaa                                                             <  1:1817574/150‑1 (MQ=255)
                                                                                       aaTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATaaa                                                            >  2:482596/1‑150 (MQ=255)
                                                                                        aTCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACACCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAg                                                           >  2:5008501/1‑150 (MQ=255)
                                                                                             gTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAgcggcg                                                      <  2:3081838/150‑1 (MQ=255)
                                                                                                gCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAat                                                   <  1:671939/150‑1 (MQ=255)
                                                                                                  tGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAatat                                                 <  2:3025366/150‑1 (MQ=255)
                                                                                                  tGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGAATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAatat                                                 <  1:1965318/150‑1 (MQ=255)
                                                                                                  gggCTGTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATACACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAatat                                                 <  1:4284231/149‑1 (MQ=255)
                                                                                                       ttGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCGGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGctct                                            <  1:142791/150‑1 (MQ=255)
                                                                                                         gCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGc                                          <  2:3465600/150‑1 (MQ=255)
                                                                                                         gCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGc                                          <  2:3614346/150‑1 (MQ=255)
                                                                                                            gTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCAcc                                       >  2:4529007/1‑150 (MQ=255)
                                                                                                                ggCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGa                                   >  1:3488287/1‑150 (MQ=255)
                                                                                                                 gCAGCGCCCCACGCCAGCCACTGGCGAAAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGAt                                  <  2:1899886/150‑1 (MQ=255)
                                                                                                                  cAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATc                                 <  2:2854242/150‑1 (MQ=255)
                                                                                                                  cAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATc                                 >  1:346484/1‑150 (MQ=255)
                                                                                                                   aGCGCCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCa                                <  2:2743342/150‑1 (MQ=255)
                                                                                                                    gcgcCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAg                               <  2:2033460/150‑1 (MQ=255)
                                                                                                                     cgcCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGc                              <  1:2094366/150‑1 (MQ=255)
                                                                                                                     cgcCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGc                              <  2:2523675/150‑1 (MQ=255)
                                                                                                                     cgcCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGc                              <  2:4145696/150‑1 (MQ=255)
                                                                                                                      gcCCCACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCa                             >  1:2527864/1‑150 (MQ=255)
                                                                                                                       ccccACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCaa                            >  1:82124/1‑150 (MQ=255)
                                                                                                                       ccccACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCaa                            >  2:3966290/1‑150 (MQ=255)
                                                                                                                       ccccACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCaa                            <  2:886293/150‑1 (MQ=255)
                                                                                                                        cccACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAAt                           >  1:2809167/1‑150 (MQ=255)
                                                                                                                          cACGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATcc                         >  2:3538387/1‑150 (MQ=255)
                                                                                                                            cGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCca                       <  2:2901642/150‑1 (MQ=255)
                                                                                                                            cGCCAGCCACTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCca                       <  1:2025472/150‑1 (MQ=255)
                                                                                                                             gccagccaCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCcac                      <  2:4241149/150‑1 (MQ=255)
                                                                                                                               cagccaCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACag                    >  2:4597079/1‑150 (MQ=255)
                                                                                                                                agccaCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACagg                   <  2:10169/150‑1 (MQ=255)
                                                                                                                                   caCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACAACTCCAGTTCAGCGGCTATCTCGAGCTCGCTCATCCCCGCCTGAATAAAGAGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACaggcga                >  2:4109724/1‑150 (MQ=255)
                                                                                                                                    aCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACaggcgag               <  1:2183712/150‑1 (MQ=255)
                                                                                                                                    aCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACaggcgag               <  1:173031/150‑1 (MQ=255)
                                                                                                                                    aCTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACaggcgag               <  2:318677/150‑1 (MQ=255)
                                                                                                                                     cTGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACaggcgagg              <  1:4638652/150‑1 (MQ=255)
                                                                                                                                      tGGCGACAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACaggcgaggc             <  1:2218015/150‑1 (MQ=255)
                                                                                                                                           aCAATGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGAtg        >  1:1255319/1‑149 (MQ=255)
                                                                                                                                               tGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTtct    >  1:312210/1‑150 (MQ=255)
                                                                                                                                               tGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTtct    <  2:1112725/150‑1 (MQ=255)
                                                                                                                                               tGGTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTtct    <  1:1143524/150‑1 (MQ=255)
                                                                                                                                                ggTGTAAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTtctc   >  1:1634551/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
ATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTCCAGTTCAGCGGCTATCTCGCGCTCGCTCATCCCCGCCTGAATAAAGCGGCGAATATGCTCTGCACCGCGATCAGCAATCCCACAGGCGAGGCGGATTTTCTCC  >  CP009273/2499419‑2499713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑