BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,524,056	C→A	100%	A179A (GCG→GCT)	zipA ←	FtsZ stabilizer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,524,056	0	C	A	100.0%	990.4 / NA	297	A179A (GCG→GCT)	zipA	FtsZ stabilizer
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (181/116); total (181/116)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 297 total aligned reads displayed.

AATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGC  >  CP009273/2523909‑2524200

  tatTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTa                                                                                                                                              >  1:4456853/1‑150 (MQ=255)

   atTCTTATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTCGCTCGCTACCTTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAc                                                                                                                                             <  2:9237/150‑1 (MQ=255)

   atTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAc                                                                                                                                             >  1:4458005/1‑150 (MQ=255)

    ttCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAca                                                                                                                                            <  2:4431110/150‑1 (MQ=255)

         atCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAcaggctc                                                                                                                                       >  2:745785/1‑150 (MQ=255)

           cGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTAcaggctcag                                                                                                                                     >  2:1344269/1‑150 (MQ=255)

                aaaaTGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCtgt                                                                                                                                >  2:3023324/1‑150 (MQ=255)

                  aaTGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTgtgg                                                                                                                              >  1:4527336/1‑150 (MQ=255)

                      aaGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGcc                                                                                                                          >  2:2103270/1‑150 (MQ=255)

                                 ttgAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTc                                                                                                               >  1:1914208/1‑150 (MQ=255)

                                   gAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTg                                                                                                             >  1:3200339/1‑150 (MQ=255)

                                       gCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAgg                                                                                                         >  1:2575365/1‑150 (MQ=255)

                                       gCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAgg                                                                                                         >  2:217988/1‑150 (MQ=255)

                                        cTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGc                                                                                                        >  2:1908119/1‑150 (MQ=255)

                                         tGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCt                                                                                                       <  2:3531977/150‑1 (MQ=255)

                                         tGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCt                                                                                                       <  2:2870790/150‑1 (MQ=255)

                                         tGTTAAGAAGCAGTTCACCGTTGAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCt                                                                                                       <  1:1361288/150‑1 (MQ=255)

                                          gTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTg                                                                                                      >  1:1772739/1‑150 (MQ=255)

                                           ttAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTgg                                                                                                     <  2:4896737/150‑1 (MQ=255)

                                                  gCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCt                                                                                              <  1:459469/150‑1 (MQ=255)

                                                   cAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCtg                                                                                             >  2:1018076/1‑150 (MQ=255)

                                                    aGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCtgt                                                                                            >  1:3078591/1‑150 (MQ=255)

                                                    aGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCtgt                                                                                            >  1:2779783/1‑150 (MQ=255)

                                                     gTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCtgtt                                                                                           >  1:312007/1‑150 (MQ=255)

                                                     gTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCtgtt                                                                                           >  1:2084523/1‑150 (MQ=255)

                                                     gTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCtgtt                                                                                           >  1:3656267/1‑150 (MQ=255)

                                                     gTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCtgtt                                                                                           >  1:4936293/1‑150 (MQ=255)

                                                      ttCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCtgttg                                                                                          <  2:3420582/150‑1 (MQ=255)

                                                           cGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCg                                                                                     >  1:1672451/1‑150 (MQ=255)

                                                             tttAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGt                                                                                   >  2:1189023/1‑150 (MQ=255)

                                                             tttAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGt                                                                                   <  1:1447348/150‑1 (MQ=255)

                                                                 gCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCg                                                                               <  2:4722964/150‑1 (MQ=255)

                                                                 gCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCg                                                                               <  2:4565374/150‑1 (MQ=255)

                                                                      cTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCt                                                                          >  2:3895129/1‑150 (MQ=255)

                                                                       tACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTg                                                                         >  1:5194880/1‑150 (MQ=255)

                                                                       tACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTg                                                                         >  2:4055154/1‑150 (MQ=255)

                                                                        acagagATGCGCCGCGACGTTCATGATAATCACCGCTTCATTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGa                                                                        >  2:4635331/6‑150 (MQ=255)

                                                                        aCCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGa                                                                        >  2:3715213/1‑150 (MQ=255)

                                                                          cGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAAt                                                                      <  2:4606793/150‑1 (MQ=255)

                                                                          cGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAAt                                                                      <  1:5216355/150‑1 (MQ=255)

                                                                           gTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATg                                                                     <  1:1292973/150‑1 (MQ=255)

                                                                            tGATGCGCCGCGACGTTCCTGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTCCAGGCTCAGGCGGTGGTGCCGCTCCGGGTTCTGCTGGCTGGAAAGCCTGTTGTGCCGGGTGCGGGGCTGACTGc                                                                    >  2:371049/1‑150 (MQ=255)

                                                                            tGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGc                                                                    >  1:1121246/1‑150 (MQ=255)

                                                                                   ccgcGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCt                                                             <  1:745785/150‑1 (MQ=255)

                                                                                    cgcgACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTg                                                            >  1:4689040/1‑150 (MQ=255)

                                                                                     gcgACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTgc                                                           <  2:872000/150‑1 (MQ=255)

                                                                                      cgACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTgcg                                                          <  2:1914208/150‑1 (MQ=255)

                                                                                      cgACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTgcg                                                          >  2:3613486/1‑150 (MQ=255)

                                                                                       gACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTgcgg                                                         >  1:4103374/1‑150 (MQ=255)

                                                                                         cGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTgcggcg                                                       >  1:2903352/1‑150 (MQ=255)

                                                                                          gTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGgcgc                                                      >  2:3277861/1‑150 (MQ=255)

                                                                                           ttCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCt                                                     <  1:1021509/150‑1 (MQ=255)

                                                                                           ttCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCt                                                     <  2:1653111/150‑1 (MQ=255)

                                                                                            tCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTg                                                    <  1:2430315/150‑1 (MQ=255)

                                                                                            tCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTg                                                    <  1:4228177/150‑1 (MQ=255)

                                                                                            tCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTg                                                    <  1:3896794/150‑1 (MQ=255)

                                                                                            tCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTg                                                    >  1:5075605/1‑150 (MQ=255)

                                                                                            tCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTg                                                    <  1:890015/150‑1 (MQ=255)

                                                                                                      cACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCtg                                          >  2:2386149/1‑150 (MQ=255)

                                                                                                      cACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCtg                                          <  2:2971831/150‑1 (MQ=255)

                                                                                                       aCCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCtgt                                         <  2:4402517/150‑1 (MQ=255)

                                                                                                       aCCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCtgt                                         <  1:1208133/150‑1 (MQ=255)

                                                                                                        ccGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCtgtg                                        <  1:3277450/150‑1 (MQ=255)

                                                                                                          gCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGc                                      >  1:2643069/1‑150 (MQ=255)

                                                                                                           cttcttTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCg                                     >  2:3685370/1‑150 (MQ=255)

                                                                                                            ttcttTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGTCGCTGGCGCGACCTGTGGCGa                                    >  2:2290119/1‑150 (MQ=255)

                                                                                                              cttTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGaaa                                  <  2:4489970/150‑1 (MQ=255)

                                                                                                               tttGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAAc                                 <  2:2575365/150‑1 (MQ=255)

                                                                                                                ttGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACt                                <  1:1859249/150‑1 (MQ=255)

                                                                                                                   cgcTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgc                             >  1:444138/1‑150 (MQ=255)

                                                                                                                    gcTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgct                            <  2:234241/150‑1 (MQ=255)

                                                                                                                    gcTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgct                            >  1:4148214/1‑150 (MQ=255)

                                                                                                                    gcTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgct                            <  2:1818582/150‑1 (MQ=255)

                                                                                                                     cTTCGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGgctg                           >  1:2849668/1‑150 (MQ=255)

                                                                                                                        cGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGctgctg                        <  2:109641/150‑1 (MQ=255)

                                                                                                                        cGGTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGctgctg                        <  2:3235104/150‑1 (MQ=255)

                                                                                                                         ggTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCtgctgc                       >  2:2110751/1‑150 (MQ=255)

                                                                                                                         ggTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCtgctgc                       >  2:4533824/1‑150 (MQ=255)

                                                                                                                          gTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCa                      >  1:2183785/1‑150 (MQ=255)

                                                                                                                          gTTTATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCAGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCa                      >  1:1023662/1‑150 (MQ=255)

                                                                                                                           tttATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCaa                     <  2:1466033/150‑1 (MQ=255)

                                                                                                                           tttATCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCaa                     <  2:1952664/150‑1 (MQ=255)

                                                                                                                              aTCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACgg                  >  2:311326/1‑150 (MQ=255)

                                                                                                                              aTCCATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACgg                  >  2:310565/1‑150 (MQ=255)

                                                                                                                                ccATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCt                >  1:4014619/1‑150 (MQ=255)

                                                                                                                                 cATAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTg               >  2:4750851/1‑150 (MQ=255)

                                                                                                                                   tAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGtt             <  2:4212356/150‑1 (MQ=255)

                                                                                                                                   tAACTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGtt             >  2:4521936/1‑150 (MQ=255)

                                                                                                                                    aaCTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGTATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTc            <  2:4176979/150‑1 (MQ=255)

                                                                                                                                    aaCTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTc            >  2:821104/1‑150 (MQ=255)

                                                                                                                                     aCTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCa           <  2:4295111/150‑1 (MQ=255)

                                                                                                                                     aCTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCa           >  1:1323497/1‑150 (MQ=255)

                                                                                                                                      cTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAg          >  2:2613589/1‑150 (MQ=255)

                                                                                                                                      cTGGAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAg          <  1:3557013/150‑1 (MQ=255)

                                                                                                                                         gAGCAGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCt       <  1:3339163/150‑1 (MQ=255)

                                                                                                                                          aGCAGGTTCAGCTACAGGCTCAGGCGGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGTAACGGCTGTTCAGGCTg      >  2:307275/1‑150 (MQ=255)

                                                                                                                                             aGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCgg   >  1:3835714/1‑150 (MQ=255)

                                                                                                                                             aGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCgg   >  2:57479/1‑150 (MQ=255)

                                                                                                                                             aGGTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCgg   >  1:1568697/1‑150 (MQ=255)

                                                                                                                                              ggTTCAGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGc  >  2:3012835/1‑150 (MQ=255)

AATATTCATATCGCCAAAAATGAAGCCCGCTTGTTGAATGCTGTTAAGAAGCAGTTCACCGTTTAGCTCGCTACCGTGATGCGCCGCGACGTTCATGATAATCACCGCTTCTTTGCGCTTCGGTTTATCCATAACTGGAGCAGGTTCCGCTACAGGCTCAGGCTGTGGTGCCGCTACGGGTTCTGCAGGCTGGAAAGCCTGTTGTGCCGGTTGCGGTGCTGAATGCACAGGCTGCGGCGCTGGCGCGACCTGTGGCGAAACTGGCTGCTGCAACGGCTGTTCAGGCTGCGGC  >  CP009273/2523909‑2524200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`