Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,555,735 G→T 8.3% R89L (CGA→CTA)  yffO → CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,555,7350GT 8.3% 1155.5 / 47.1 337R89L (CGA→CTA) yffOCPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (133/175);  new base T (18/11);  total (151/186)
Fisher's exact test for biased strand distribution p-value = 7.72e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

Only 100 of 347 total aligned reads displayed.

ATTGGTAAGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTCACCATTTGGAGATGACAAACAAT  >  CP009273/2555586‑2555884
                                                                                                                                                     |                                                                                                                                                     
aTTGGTAAGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCg                                                                                                                                                       >  1:3702414/1‑150 (MQ=255)
aTTGGTAAGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCg                                                                                                                                                       <  2:4267023/150‑1 (MQ=255)
aTTGGTAAGCGAAGGTTATAGTGTGCGCAAGCTCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCg                                                                                                                                                       <  2:4909679/150‑1 (MQ=255)
   ggTAAGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACg                                                                                                                                                    <  2:530481/150‑1 (MQ=255)
       aGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGcc                                                                                                                                                <  1:2078043/150‑1 (MQ=255)
       aGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGcc                                                                                                                                                <  1:1617880/150‑1 (MQ=255)
          gAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTc                                                                                                                                             >  2:2229204/1‑150 (MQ=255)
           aaGGTTATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCg                                                                                                                                            >  1:3859790/1‑150 (MQ=255)
               ttATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGAt                                                                                                                                        >  2:3388850/1‑150 (MQ=255)
               ttATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGAt                                                                                                                                        <  2:781929/150‑1 (MQ=255)
               ttATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGAt                                                                                                                                        <  1:1718076/150‑1 (MQ=255)
                   aGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATc                                                                                                                                    <  1:2931338/150‑1 (MQ=255)
                    gTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATcg                                                                                                                                   >  1:1558733/1‑150 (MQ=255)
                    gTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATcg                                                                                                                                   <  1:2753755/150‑1 (MQ=255)
                      ttGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATcgac                                                                                                                                 <  2:3641213/150‑1 (MQ=255)
                            aaGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGtt                                                                                                                           >  1:4715276/1‑150 (MQ=255)
                            aaGATCAGCATGAGGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCTTATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGtt                                                                                                                           <  2:4996948/150‑1 (MQ=255)
                             aGATCAGCATGATGCCAGGGTTGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATACTCGACGAGGTTg                                                                                                                          <  2:3707403/150‑1 (MQ=255)
                              taTCAGAATTTTGCCAGGTATGCCTAGCAACCGTAAGATTATGCGCTGGCAACTGGATAATATGGAATTCAGGTATGGGATTGCCTGGATGTGGTGGCTATGGTGCGCTGAAGCTGGCCGAGGTGCCGTCGAGATAATCGACGAGGTTGa                                                                                                                         <  1:1949022/149‑1 (MQ=255)
                              gATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGa                                                                                                                         >  2:1050766/1‑150 (MQ=255)
                               aTCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGat                                                                                                                        >  1:1749552/1‑150 (MQ=255)
                                 cAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGatat                                                                                                                      <  2:2794199/150‑1 (MQ=255)
                                     atgatgCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAAc                                                                                                                  >  1:5216438/1‑150 (MQ=255)
                                      tgatgCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACg                                                                                                                 <  1:5259998/150‑1 (MQ=255)
                                        atgCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCa                                                                                                               >  2:384626/1‑150 (MQ=255)
                                           ccAGGGATGCCGAGCCTCCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCTGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCagag                                                                                                            <  2:4803088/150‑1 (MQ=255)
                                           ccAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCagag                                                                                                            >  2:3908913/1‑150 (MQ=255)
                                                gATGCCGAGCCACCGTCAGATGAGGCGCTGGCAACTGGAGCATATGGACTTCTGGGAAGGGATAGCCTGGATGTCGTGGATATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATgg                                                                                                       <  1:4546378/150‑1 (MQ=255)
                                                 ttGCCGTGCCACCGTCTTTTGCTGCGCTGGCAACTGTAGCATATGTACTTCAGGGAAGGGATAGCGTGTTTGTCGGGGGTTTGGTGCGCTTAAGGTGGCCGACGTGCCGTCGATATACTCGACGAGGTTGATATAAACGCAGAGGATggg                                                                                                      <  1:1836132/149‑1 (MQ=255)
                                                    ccGCGCCACCGTCAGATGATGCGCTGGCAACTGGAGAATATGGACTTCTGGGATGGGATAGCCTGGTTGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCg                                                                                                   <  1:3583531/150‑1 (MQ=255)
                                                    ccGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCg                                                                                                   <  2:2717964/150‑1 (MQ=255)
                                                          cACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAg                                                                                             <  2:2102118/150‑1 (MQ=255)
                                                           aCCGTCAGATGCTGCGCTGGCAACTGGAGCATATGGACTTCAGGGACGGGATAGCCTGGATGTCGTGGCTATGGTGCGCGGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGt                                                                                            <  2:2876384/150‑1 (MQ=255)
                                                               tCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGACGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTgcg                                                                                        <  1:3351771/150‑1 (MQ=255)
                                                                  gatgatGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCaa                                                                                     >  2:2186450/1‑150 (MQ=255)
                                                                    ttatgCGCTTGCAAATGTATAATTTGTATTTCAGGGATGTGAGCGCTTGTTTGTCTTGGATTTTGTGCTATGAAGCTTGCCGAGGTGACTTGGATATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGAAGTTGCGCTAAg                                                                                   <  2:4471444/148‑1 (MQ=255)
                                                                     gatgCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCTACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGc                                                                                  >  2:133889/1‑150 (MQ=255)
                                                                       tgCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAg                                                                                <  2:1557467/150‑1 (MQ=255)
                                                                          gcTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAg                                                                             <  2:2944898/150‑1 (MQ=255)
                                                                           cTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAgc                                                                            <  1:45803/150‑1 (MQ=255)
                                                                            tGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAgca                                                                           >  2:4505849/1‑150 (MQ=255)
                                                                               cAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAgcaaag                                                                        <  1:2404902/150‑1 (MQ=255)
                                                                               cAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAgcaaag                                                                        <  2:24287/150‑1 (MQ=255)
                                                                               cAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAgcaaag                                                                        >  2:3778663/1‑150 (MQ=255)
                                                                                 aCTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCTACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAgcaaaggc                                                                      <  1:2646974/150‑1 (MQ=255)
                                                                                 aCTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAgcaaaggc                                                                      <  1:4373483/150‑1 (MQ=255)
                                                                                   tGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAgcaaaggcaa                                                                    <  2:4371541/150‑1 (MQ=255)
                                                                                   tGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAgcaaaggcaa                                                                    <  2:3439673/150‑1 (MQ=255)
                                                                                    ggAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAgcaaaggcaaa                                                                   <  1:68983/150‑1 (MQ=255)
                                                                                     gAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAgcaaaggcaaag                                                                  <  1:1271622/150‑1 (MQ=255)
                                                                                             ggACTTCAGGGATGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCactact                                                          <  1:884111/150‑1 (MQ=255)
                                                                                             ggACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGGGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCactact                                                          <  1:2105623/150‑1 (MQ=255)
                                                                                                cTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGgc                                                       <  2:3562997/150‑1 (MQ=255)
                                                                                                   cAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGgcagc                                                    >  1:5316433/1‑150 (MQ=255)
                                                                                                    cgggaagggaTAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGgcagca                                                   >  2:1158504/2‑150 (MQ=255)
                                                                                                    agggaagggaTAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGgcagca                                                   >  2:2646867/1‑150 (MQ=255)
                                                                                                    agggaagggaTAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGgcagca                                                   >  2:834178/1‑150 (MQ=255)
                                                                                                    agggaagggaTAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGgcagca                                                   >  1:4047613/1‑150 (MQ=255)
                                                                                                    agggaagggaTAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGgcagca                                                   >  1:3619986/1‑150 (MQ=255)
                                                                                                    agggaagggaTAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGgcagca                                                   >  1:3251937/1‑150 (MQ=255)
                                                                                                    agggaagggaTAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGgcagca                                                   >  1:2234805/1‑150 (MQ=255)
                                                                                                       gaagggaTAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGcc                                                <  2:2667941/150‑1 (MQ=255)
                                                                                                        aagggaTAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCa                                               >  2:185628/1‑150 (MQ=255)
                                                                                                         agggaTAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCaa                                              >  2:1845296/1‑150 (MQ=255)
                                                                                                           ggaTAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGc                                            >  1:3811974/1‑150 (MQ=255)
                                                                                                            gaTAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCt                                           <  1:1114610/150‑1 (MQ=255)
                                                                                                             aTAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTg                                          >  2:5212401/1‑150 (MQ=255)
                                                                                                                 ccTGGATGTCGTGGCTTTGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATa                                      <  1:2178131/150‑1 (MQ=255)
                                                                                                                   tGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGt                                    >  1:4680098/1‑150 (MQ=255)
                                                                                                                   tGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGt                                    >  1:1853368/1‑150 (MQ=255)
                                                                                                                    ggATGTCGTGGCTATGGTGCGCTGTAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGtt                                   <  2:3105662/150‑1 (MQ=255)
                                                                                                                     gctGTCGTGGCTTTGGTGCGCTGAAGCGGGCCGACGTGCCGTCGAGATACTCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGttt                                  <  1:1449564/148‑1 (MQ=255)
                                                                                                                     gATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGttt                                  <  2:1768621/150‑1 (MQ=255)
                                                                                                                          cGTGGCTTTGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAAc                             <  2:100973/150‑1 (MQ=255)
                                                                                                                          cGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAAc                             >  1:5057802/1‑150 (MQ=255)
                                                                                                                           gTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACa                            <  2:1968440/150‑1 (MQ=255)
                                                                                                                           gTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACa                            >  1:1017159/1‑150 (MQ=255)
                                                                                                                           gTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACa                            >  2:720487/1‑150 (MQ=255)
                                                                                                                             ggCTATGGTGCGCTGAAGCTGGCCTACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACAtt                          >  1:2662888/1‑150 (MQ=255)
                                                                                                                              gCTATGGTGCGCTGAAGCTGGCCTACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTc                         <  1:1087250/150‑1 (MQ=255)
                                                                                                                              gCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTc                         <  2:4727738/150‑1 (MQ=255)
                                                                                                                                ttgggTGCGCTGAAGCGGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTCAc                       <  1:4907065/147‑1 (MQ=255)
                                                                                                                                tATGGTGCGCTGAAGCTGGCCTACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTCAc                       >  2:3261404/1‑150 (MQ=255)
                                                                                                                                tATGGTGCGCTGAAGCTGGCCTACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTCAc                       >  1:397418/1‑150 (MQ=255)
                                                                                                                                tATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTCAc                       >  1:4253262/1‑150 (MQ=255)
                                                                                                                                tATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTCAc                       >  2:2323720/1‑150 (MQ=255)
                                                                                                                                 aTGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTCAcc                      >  1:5166161/1‑150 (MQ=255)
                                                                                                                                     tGCGCTGAAGCTGGCCTACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTCACCAttt                  <  1:1314666/150‑1 (MQ=255)
                                                                                                                                        gcTGATGCTGGCCGAGGTGCGGTCGAGATTCTGGACGAGGTTGATATATACGGAGTGGTTGGGCCGATGCAGTTGCGCATAGCAGAAGCATAGGCAAAGGCTCTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTCACCATTTGga               <  2:2440864/150‑1 (MQ=255)
                                                                                                                                        gcTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGACGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTCACCATTTGga               >  2:5116589/1‑150 (MQ=255)
                                                                                                                                          tGAAGCTGGCCTACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTCACCATTTGgaga             >  2:4095720/1‑150 (MQ=255)
                                                                                                                                          tGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTCACCATTTGgaga             >  1:2471770/1‑150 (MQ=255)
                                                                                                                                            aaGCTGGCCTACGTGCCGTCGAGATACTCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTCACCATTTGGAGATg           <  1:3480357/150‑1 (MQ=255)
                                                                                                                                            aaGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTCACCATTTGGAGATg           >  2:2585955/1‑150 (MQ=255)
                                                                                                                                             aGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTCACCATTTGGAGATGa          <  1:4799155/150‑1 (MQ=255)
                                                                                                                                               cTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTCACCATTTGGAGATGaca        >  2:2218776/1‑150 (MQ=255)
                                                                                                                                                tGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTCACCATTTGGAGATGacaa       >  2:650409/1‑150 (MQ=255)
                                                                                                                                                  gCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTCACCATTTGGAGATGacaaac     <  2:4645818/150‑1 (MQ=255)
                                                                                                                                                    cGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTCACCATTTGGAGATGacaaacaa   >  1:4146639/1‑150 (MQ=255)
                                                                                                                                                     gACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTCACCATTTGGAGATGACAAACAAt  <  1:2018105/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATTGGTAAGCGAAGGTTATAGTTTGCGCAAGATCAGCATGATGCCAGGGATGCCGAGCCACCGTCAGATGATGCGCTGGCAACTGGAGCATATGGACTTCAGGGAAGGGATAGCCTGGATGTCGTGGCTATGGTGCGCTGAAGCTGGCCGACGTGCCGTCGAGATAATCGACGAGGTTGATATAAACGCAGAGGATGGGCCGAAGCAGTTGCGCAAAGCAGAAGCAAAGGCAAAGGCACTACTGGCAGCAGCCAAGCTGAATAGTTTGAAACATTCACCATTTGGAGATGACAAACAAT  >  CP009273/2555586‑2555884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑