Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,568,720 C→T 100% A33T (GCC→ACC)  eutP ← putative P‑loop NTPase ethanolamine utilization protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,568,7200CT99.7% 915.7 / ‑4.7 280A33T (GCC→ACC) eutPputative P‑loop NTPase ethanolamine utilization protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (132/147);  total (133/147)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 280 total aligned reads displayed.

GCAACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAGCGTATAATTTAATA  >  CP009273/2568573‑2568866
                                                                                                                                                   |                                                                                                                                                  
  aaCCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCtgt                                                                                                                                                >  1:711868/1‑150 (MQ=255)
  aaCCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCtgt                                                                                                                                                >  1:622772/1‑150 (MQ=255)
  aaCCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCtgt                                                                                                                                                >  1:462768/1‑150 (MQ=255)
  aaCCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCtgt                                                                                                                                                >  1:3874099/1‑150 (MQ=255)
  aaCCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCtgt                                                                                                                                                >  2:5084541/1‑150 (MQ=255)
  aaCCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCtgt                                                                                                                                                <  1:3590185/150‑1 (MQ=255)
      gACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGttt                                                                                                                                            <  2:3614503/150‑1 (MQ=255)
      gACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGttt                                                                                                                                            <  2:1345031/150‑1 (MQ=255)
      gACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGttt                                                                                                                                            >  1:19248/1‑150 (MQ=255)
       aCTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGtttt                                                                                                                                           <  1:4184220/150‑1 (MQ=255)
       aCTTTACGGGTCATTTGCTCCATGTACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGAATCAATATCGCCCTTATCATTAAATACCACGGTCTGTGtttt                                                                                                                                           >  2:2399145/1‑150 (MQ=255)
         tttCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTc                                                                                                                                         >  2:5138387/1‑150 (MQ=255)
         tttCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTc                                                                                                                                         <  2:3834291/150‑1 (MQ=255)
          ttCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCt                                                                                                                                        <  1:1188018/150‑1 (MQ=255)
           tCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTg                                                                                                                                       <  2:2843575/150‑1 (MQ=255)
           tCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTg                                                                                                                                       <  2:639125/150‑1 (MQ=255)
            ccGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTgg                                                                                                                                      <  2:4668551/150‑1 (MQ=255)
              gggTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCg                                                                                                                                    <  1:3810752/150‑1 (MQ=255)
              gggTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCg                                                                                                                                    <  1:1620338/150‑1 (MQ=255)
               ggTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCATCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGa                                                                                                                                   >  2:438519/1‑150 (MQ=255)
               ggTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGa                                                                                                                                   >  2:1474515/1‑150 (MQ=255)
               ggTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGa                                                                                                                                   >  2:4494041/1‑150 (MQ=255)
               ggTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGAGATACCAGCGGGGATGGGTAAAATATTCACCCGGCGACTCAATATCGCCATAATCATAAAATTCCACGGTCTGTGTATTTCAGACGa                                                                                                                                   >  2:1384998/1‑150 (MQ=255)
                gTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAg                                                                                                                                  <  1:4442993/150‑1 (MQ=255)
                  cATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAggg                                                                                                                                >  2:4960901/1‑150 (MQ=255)
                     ttGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGtat                                                                                                                             <  1:5215293/150‑1 (MQ=255)
                      tGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGtata                                                                                                                            >  1:2676933/1‑150 (MQ=255)
                        cgcCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAAt                                                                                                                          <  1:4680163/150‑1 (MQ=255)
                           cATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTcc                                                                                                                       <  2:2715168/150‑1 (MQ=255)
                            aTGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTccc                                                                                                                      <  1:2237868/150‑1 (MQ=255)
                              ggACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCt                                                                                                                    <  2:2810785/150‑1 (MQ=255)
                              ggACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCt                                                                                                                    >  1:2585526/1‑150 (MQ=255)
                                  aTAGATCAGCCTTTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGGTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCaa                                                                                                                >  2:2117147/1‑150 (MQ=255)
                                     gATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGc                                                                                                             >  2:2939457/1‑150 (MQ=255)
                                      aTCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCa                                                                                                            >  2:2135661/1‑150 (MQ=255)
                                       tCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCAt                                                                                                           >  2:2211112/1‑150 (MQ=255)
                                            aTGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAAt                                                                                                      >  2:716253/1‑150 (MQ=255)
                                            aTGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAAt                                                                                                      <  2:1997436/150‑1 (MQ=255)
                                             tGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATa                                                                                                     >  2:4831624/1‑150 (MQ=255)
                                              gTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATaa                                                                                                    >  1:505525/1‑150 (MQ=255)
                                               tCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAAc                                                                                                   <  2:3272324/150‑1 (MQ=255)
                                                cTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACg                                                                                                  <  1:1743530/150‑1 (MQ=255)
                                                    aTCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACgttgt                                                                                              >  2:3069987/1‑150 (MQ=255)
                                                      ccTGTAGCTTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACACGGCGTATCAATATCTCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGttt                                                                                            <  2:971355/150‑1 (MQ=255)
                                                      ccTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGttt                                                                                            <  1:429246/150‑1 (MQ=255)
                                                        tGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGttttt                                                                                          <  1:1399955/150‑1 (MQ=255)
                                                          tAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTcc                                                                                        <  2:1216549/150‑1 (MQ=255)
                                                           aGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCt                                                                                       >  1:4470348/1‑150 (MQ=255)
                                                            gCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTg                                                                                      <  1:1659526/150‑1 (MQ=255)
                                                            gCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTg                                                                                      <  2:1551605/150‑1 (MQ=255)
                                                              gTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCTAGGGTATAATTCCCCTGCAACGCATTAAATAAAGTTGTTTTTcattac                                                                                    >  1:3646120/1‑145 (MQ=255)
                                                              gTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGcc                                                                                    <  2:624290/150‑1 (MQ=255)
                                                              gTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGcc                                                                                    <  1:4134123/150‑1 (MQ=255)
                                                                ggTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGcccc                                                                                  <  1:2127383/150‑1 (MQ=255)
                                                                ggTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGcccc                                                                                  <  2:3748233/150‑1 (MQ=255)
                                                                  tAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGa                                                                                >  1:5120555/1‑150 (MQ=255)
                                                                   aaTTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGTGTATAATTCCCCTGCAACGCATTAAATAACTTTGTTTTTCCTGCACCGAc                                                                               >  2:4141169/1‑150 (MQ=255)
                                                                   aaTTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGAc                                                                               <  1:837453/150‑1 (MQ=255)
                                                                   aaTTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGAc                                                                               >  1:3184491/1‑150 (MQ=255)
                                                                   aaTTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGAc                                                                               >  2:4745938/1‑150 (MQ=255)
                                                                          gCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTAccc                                                                        >  2:1933079/1‑150 (MQ=255)
                                                                               aTACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACaaa                                                                   >  2:4090783/1‑150 (MQ=255)
                                                                                tACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAAc                                                                  <  2:3932308/150‑1 (MQ=255)
                                                                                     gCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAAt                                                             <  2:18065/150‑1 (MQ=255)
                                                                                        gggATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCg                                                          >  1:1542419/1‑150 (MQ=255)
                                                                                           aTGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGttt                                                       <  1:58900/150‑1 (MQ=255)
                                                                                            tGGTTTAAATATTCACCCGGCGTATCAATCTCGCCCTTATCATTATATTCCTCGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCGTGCTACGAATTAATTTACGTTGTTTTTCCTGCCCCGTCGATTCCCACACACGCAATTCGTTTc                                                      <  2:3309646/150‑1 (MQ=255)
                                                                                              gTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCAt                                                    <  2:2341262/150‑1 (MQ=255)
                                                                                              gTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCAt                                                    <  2:818421/150‑1 (MQ=255)
                                                                                                tAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGa                                                  >  2:3385206/1‑150 (MQ=255)
                                                                                                 aaaaTATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGaa                                                 >  1:2491248/1‑150 (MQ=255)
                                                                                                   aaTATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATg                                               >  2:4312101/1‑150 (MQ=255)
                                                                                                   aaTATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATg                                               >  2:367351/1‑150 (MQ=255)
                                                                                                    atatTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGc                                              <  1:2128893/150‑1 (MQ=255)
                                                                                                        tCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCg                                          <  1:145364/150‑1 (MQ=255)
                                                                                                        tCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCg                                          <  1:898718/150‑1 (MQ=255)
                                                                                                         cACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGt                                         >  2:575211/1‑150 (MQ=255)
                                                                                                         cACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGt                                         >  2:4438467/1‑150 (MQ=255)
                                                                                                         cACCCGGCGTATCAATATCGCCCTTATCATTAAATTACACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGt                                         >  2:2608347/1‑150 (MQ=255)
                                                                                                           cccGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTAt                                       >  2:1253219/1‑150 (MQ=255)
                                                                                                                 gTATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACtt                                 <  2:1191083/150‑1 (MQ=255)
                                                                                                                  tATCAATATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACttt                                >  2:4413852/1‑150 (MQ=255)
                                                                                                                      aaTATCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGt                            >  1:4826509/1‑150 (MQ=255)
                                                                                                                        tatCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCa                          <  1:926442/150‑1 (MQ=255)
                                                                                                                        tatCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCa                          <  1:455513/150‑1 (MQ=255)
                                                                                                                        tatCGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCa                          <  1:3440443/150‑1 (MQ=255)
                                                                                                                           cGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCAttt                       <  1:2723421/150‑1 (MQ=255)
                                                                                                                           cGCCCTTATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCAttt                       <  2:5286297/150‑1 (MQ=255)
                                                                                                                                ttATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACaa                  >  1:2648456/1‑150 (MQ=255)
                                                                                                                                 tATCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACaaa                 >  2:595752/1‑150 (MQ=255)
                                                                                                                                  aTCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAg                <  1:2958569/150‑1 (MQ=255)
                                                                                                                                  aTCATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAg                <  1:4751385/150‑1 (MQ=255)
                                                                                                                                    cATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAGCg              <  1:2997147/150‑1 (MQ=255)
                                                                                                                                    cATTAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAGCg              <  1:1254542/150‑1 (MQ=255)
                                                                                                                                      ttAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAGCGta            >  1:1208000/1‑150 (MQ=255)
                                                                                                                                       tAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAGCGtat           >  2:2262038/1‑150 (MQ=255)
                                                                                                                                       tAAATTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAGCGtat           <  1:2826088/150‑1 (MQ=255)
                                                                                                                                          aTTCCACGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAGCGTATAAt        <  2:5233092/150‑1 (MQ=255)
                                                                                                                                                cGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAGCGTATAATTtaata  <  1:4077843/150‑1 (MQ=255)
                                                                                                                                                cGGTCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAGCGTATAATTtaata  >  1:2364653/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCAACCGACTTTCCGGGTCATTTGCGCCATGGACATAGATCAGCATGTCTACATCCTGTAGCGTGGTAATTAAGGCGTGATACCAGCGGGGATGGTTAAAATATTCACCCGGCGTATCAATATCGCCCTTATCATTAAATTCCACGGCCTGTGTTTTTCTGGCGAGGGTATAATTCCCCTGCAACGCATTAAATAACGTTGTTTTTCCTGCCCCGACGCTACCCACAAACGCAATTCGTTTCATGAATGCCTCGTATTAACTTTTGGTCATTTCACAAAGCGTATAATTTAATA  >  CP009273/2568573‑2568866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑