Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,576,325 C→A 100% E171D (GAG→GAT)  nudK ← GDP‑mannose pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,576,3250CA99.7% 934.3 / ‑4.5 286E171D (GAG→GAT) nudKGDP‑mannose pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (152/132);  total (153/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 291 total aligned reads displayed.

GTATCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCC  >  CP009273/2576178‑2576471
                                                                                                                                                   |                                                                                                                                                  
gTATCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTAtatc                                                                                                                                                  >  1:2440177/1‑150 (MQ=255)
gTATCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTAtatc                                                                                                                                                  >  2:2177537/1‑150 (MQ=255)
         ttGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGtttt                                                                                                                                         >  1:4206375/1‑150 (MQ=255)
                ggTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGatcatc                                                                                                                                  >  1:4321601/1‑150 (MQ=255)
                 gTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCAtct                                                                                                                                 <  2:258715/150‑1 (MQ=255)
                  tAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCAtctc                                                                                                                                <  2:4862545/150‑1 (MQ=255)
                  tAGCGCGGCTAAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCAtctc                                                                                                                                <  1:1181115/150‑1 (MQ=255)
                   aGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTcc                                                                                                                               <  1:3697099/150‑1 (MQ=255)
                       cgGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATAATCTCCAgcg                                                                                                                           >  1:3001956/1‑150 (MQ=255)
                                cGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGa                                                                                                                  >  2:2337400/1‑150 (MQ=255)
                                  gATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAAc                                                                                                                >  2:1127477/1‑150 (MQ=255)
                                  gATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAAc                                                                                                                >  2:2556368/1‑150 (MQ=255)
                                        tCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGc                                                                                                          >  1:2000341/1‑150 (MQ=255)
                                         cGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCCGCGCCTGGCTGAACGGCAGCt                                                                                                         >  1:2339245/1‑150 (MQ=255)
                                          ggATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTc                                                                                                        <  1:4430014/150‑1 (MQ=255)
                                           gATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCg                                                                                                       >  1:3548302/1‑150 (MQ=255)
                                                aGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCAc                                                                                                  >  1:3816966/1‑150 (MQ=255)
                                                 gCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACt                                                                                                 <  2:5239863/150‑1 (MQ=255)
                                                    aataatAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCa                                                                                              <  1:3850402/150‑1 (MQ=255)
                                                    aataatAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCa                                                                                              <  1:2633681/150‑1 (MQ=255)
                                                     ataataAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCaa                                                                                             >  2:4316817/1‑150 (MQ=255)
                                                      taataaACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAat                                                                                            >  2:2835827/1‑150 (MQ=255)
                                                      taataaACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCGCGAGCACTGCAat                                                                                            >  1:2922508/1‑150 (MQ=255)
                                                       aataaACAACGAAATATTATTGTTATTTTTCATTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTAGAGCACTTAAata                                                                                           >  2:766130/1‑150 (MQ=255)
                                                        ataaACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAatat                                                                                          >  1:1070819/1‑150 (MQ=255)
                                                          aaaCAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatct                                                                                        >  2:147521/1‑150 (MQ=255)
                                                          aaaCAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatct                                                                                        <  1:2886363/150‑1 (MQ=255)
                                                          aaaCAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatct                                                                                        >  1:4289175/1‑150 (MQ=255)
                                                           aacaacGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatctt                                                                                       >  1:525202/1‑150 (MQ=255)
                                                            acaacGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttc                                                                                      >  2:4105883/1‑150 (MQ=255)
                                                            acaacGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttc                                                                                      <  1:1677818/150‑1 (MQ=255)
                                                            acaacGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttc                                                                                      >  2:578579/1‑150 (MQ=255)
                                                              aacGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttcat                                                                                    <  2:4929231/150‑1 (MQ=255)
                                                              aacGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttcat                                                                                    <  1:3211350/150‑1 (MQ=255)
                                                              aacGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttcat                                                                                    <  2:3085242/150‑1 (MQ=255)
                                                               acGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttcatc                                                                                   >  2:388166/1‑150 (MQ=255)
                                                               acGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttcatc                                                                                   >  1:2131550/1‑150 (MQ=255)
                                                                cGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttcatct                                                                                  >  2:511676/1‑150 (MQ=255)
                                                                 gAAATATTATTGTTATTTTTCAGTCGATTTAATGTGACGTTTGCTAATAGTTAAGCAATTACTGCGTCTTAGCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCGGGCTGAACGGCAGCTCGAGCACTTCAATatcttcatctt                                                                                 <  2:3620007/150‑1 (MQ=255)
                                                                 gAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttcatctt                                                                                 <  2:4386318/150‑1 (MQ=255)
                                                                 gAAATATTAGTGTTATTTTTCTGTCGATTAAATGTGACGTTTGCAAAGAGTTAAGCAATAACACCGTCTTACCGTCACGTATTTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttcatctt                                                                                 <  1:1456304/150‑1 (MQ=255)
                                                                  aaaTATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttcatcttc                                                                                <  1:2553225/150‑1 (MQ=255)
                                                                  aaaTATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATatcttcatcttc                                                                                <  1:2206032/150‑1 (MQ=255)
                                                                   aaTATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCg                                                                               >  1:319031/1‑150 (MQ=255)
                                                                      attattGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCATCTCGAGCACTTCAATATCTTCATCTTAGaca                                                                            >  2:3462813/1‑150 (MQ=255)
                                                                        tattGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACAcc                                                                          <  1:141916/150‑1 (MQ=255)
                                                                        tattGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACAcc                                                                          <  1:726563/150‑1 (MQ=255)
                                                                        tattGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACAcc                                                                          >  2:4978918/1‑150 (MQ=255)
                                                                        tattGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGAAATAACACAGTCTTACCGTCACGTATATCGAAGTTTTTGATCATCTACATCGACTGGCTGAACGGCAGCTCGAGAACTTCAATATCTTCATCTTCGacaac                                                                          >  2:2717348/1‑148 (MQ=255)
                                                                         attGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCg                                                                         >  1:2561147/1‑150 (MQ=255)
                                                                           tGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGcc                                                                       >  1:4699792/1‑150 (MQ=255)
                                                                           tGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGcc                                                                       >  1:35089/1‑150 (MQ=255)
                                                                                tttttCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCgg                                                                  >  1:1001852/1‑150 (MQ=255)
                                                                                   ttCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTTAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGt                                                               >  2:3738269/1‑150 (MQ=255)
                                                                                    tCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGtt                                                              <  1:1204478/150‑1 (MQ=255)
                                                                                     cAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTa                                                             <  2:3532362/150‑1 (MQ=255)
                                                                                      aGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAg                                                            >  1:3354006/1‑150 (MQ=255)
                                                                                       gTCCATTATATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAgc                                                           <  1:4807203/150‑1 (MQ=255)
                                                                                       gTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAgc                                                           <  2:2406728/150‑1 (MQ=255)
                                                                                       gTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGGTAgc                                                           >  1:4632674/1‑150 (MQ=255)
                                                                                        tCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAgcg                                                          <  1:2009892/150‑1 (MQ=255)
                                                                                        tCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAgcg                                                          >  1:853500/1‑150 (MQ=255)
                                                                                          cATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAgcgcg                                                        >  2:4654910/1‑150 (MQ=255)
                                                                                             tAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCTCCGGTTTTTAACATCACCATCGCCTGGCAGAAAGGCAGCACGAGCACATCAATATCTTCATCTTCGACACCGCCACCGGCTTTATCGCGCTg                                                     >  1:2954778/1‑150 (MQ=255)
                                                                                             tAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTg                                                     <  1:1771652/150‑1 (MQ=255)
                                                                                             tAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTg                                                     <  2:222645/150‑1 (MQ=255)
                                                                                              aaaTGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGa                                                    >  2:166542/1‑150 (MQ=255)
                                                                                                 tgtgACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTg                                                 <  2:4248473/150‑1 (MQ=255)
                                                                                                  gtgACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGt                                                <  1:4055725/150‑1 (MQ=255)
                                                                                                      cGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCCCGTATATCGCCGGTTTTGATCATCTCCAGCGCCAGGCTGAACGGCAGCTCGAGCACTTCAATCTCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATGGTCACt                                            >  2:1746344/1‑150 (MQ=255)
                                                                                                      cGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACt                                            <  2:4131/150‑1 (MQ=255)
                                                                                                       gTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTg                                           >  1:3046116/1‑150 (MQ=255)
                                                                                                        tttGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGt                                          <  2:4367851/150‑1 (MQ=255)
                                                                                                          tGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTAt                                        >  1:587541/1‑150 (MQ=255)
                                                                                                           gCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTAtt                                       <  1:5190215/150‑1 (MQ=255)
                                                                                                            cAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTc                                      <  2:683432/150‑1 (MQ=255)
                                                                                                            cAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTc                                      <  2:2027967/150‑1 (MQ=255)
                                                                                                             aaaTAGTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCg                                     >  1:2755241/1‑150 (MQ=255)
                                                                                                                  gTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGAt                                <  1:452222/150‑1 (MQ=255)
                                                                                                                  gTTAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGAt                                >  2:470174/1‑150 (MQ=255)
                                                                                                                   ttAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATa                               <  1:1861388/150‑1 (MQ=255)
                                                                                                                   ttAAGCAATAACACCGTCGTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATa                               <  2:4550800/150‑1 (MQ=255)
                                                                                                                    tAAGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATaa                              >  2:1611178/1‑150 (MQ=255)
                                                                                                                     aaGCAATAACACCGTCTTACCGTCACGTATATCGCCGGGTTATGTCATCTCCAGCGCCAGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCAAATTCGACACCGCCCCCCGCGGTAACGCGCAGATATTCACTGTATTCGGCGATaaa                             >  1:5128947/1‑150 (MQ=255)
                                                                                                                      aGCAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACGTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGAGTGTCTCTGTATTCGGCGATaaaa                            >  2:3301422/1‑150 (MQ=255)
                                                                                                                        cAATAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATaaaaaa                          >  2:2655226/1‑150 (MQ=255)
                                                                                                                         aaTAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAg                         >  2:3835500/1‑150 (MQ=255)
                                                                                                                         aaTAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAg                         <  1:1526926/150‑1 (MQ=255)
                                                                                                                          aTAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGt                        >  2:5022347/1‑150 (MQ=255)
                                                                                                                          aTAACACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCGGAATGTCACTGTATTCGGCGATAAAAAAGt                        >  1:1347597/1‑150 (MQ=255)
                                                                                                                            aaCACCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTgg                      <  1:4123888/150‑1 (MQ=255)
                                                                                                                            aaCACCGACTTACCGTCACGTATATCGCCGGTTTAGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCCCCGGCGTTAGCGCGCAGATTGTCACTGTATTCGGCGAGACAAAAGTgg                      >  2:5323397/1‑150 (MQ=255)
                                                                                                                             acacCGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGa                     <  1:3096018/150‑1 (MQ=255)
                                                                                                                                ccGTCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTa                  <  2:3206938/150‑1 (MQ=255)
                                                                                                                                   tCTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTATGCGATAAAAAATTGGATTAGCt               >  1:3761788/1‑150 (MQ=255)
                                                                                                                                    cTTACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTc              <  2:3762450/150‑1 (MQ=255)
                                                                                                                                     ttACCGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCa             >  2:1023910/1‑150 (MQ=255)
                                                                                                                                        acGTCACGTATATCGCCGGTTTTGATCAGCTCCAGCGCCTGGCTGTAAGGCAGGTCGAGCACTTCTATTTGTTCATCTTCGACACCGCCACCGGCGTTAGCGCGATGTTTGTCTCTGTTTTCGGCGATAAATAAGTGGATTAGCTCAGTc          <  2:1405786/149‑1 (MQ=255)
                                                                                                                                         cGTCACGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTca         >  1:646123/1‑150 (MQ=255)
                                                                                                                                             aCGTATATCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACAcc     <  2:2000341/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTATCTTCGTTGCGCAGGTAGCGCGGCTCAATCGGATTTGTCGGATTGAGCCAATAATAAACAACGAAATATTATTGTTATTTTTCAGTCCATTAAATGTGACGTTTGCAAATAGTTAAGCAATAACACCGTCTTACCGTCACGTATCTCGCCGGTTTTGATCATCTCCAGCGCCTGGCTGAACGGCAGCTCGAGCACTTCAATATCTTCATCTTCGACACCGCCACCGGCGTTAGCGCGCTGATTGTCACTGTATTCGGCGATAAAAAAGTGGATTAGCTCAGTCACACCGCC  >  CP009273/2576178‑2576471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑