Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,601,971 C→A 9.3% I42I (ATC→ATA)  hyfG → hydrogenase 4, subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,601,9710CA 9.3% 1050.9 / 48.8 299I42I (ATC→ATA) hyfGhydrogenase 4, subunit
Reads supporting (aligned to +/- strand):  ref base C (129/142);  new base A (12/16);  total (141/158)
Fisher's exact test for biased strand distribution p-value = 6.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 332 total aligned reads displayed.

CGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGT  >  CP009273/2601823‑2602120
                                                                                                                                                    |                                                                                                                                                     
 gCATCTGTTTCGGAGAAACACAGTGAACGTTAAATCAGCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCAATACGGTGAAaatcaa                                                                                                                                                     >  1:727414/1‑150 (MQ=255)
  cATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAaatcaat                                                                                                                                                    <  1:3382124/150‑1 (MQ=255)
  cATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAaatcaat                                                                                                                                                    <  1:1698934/150‑1 (MQ=255)
   aTCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAGGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAaatcaatc                                                                                                                                                   >  2:371585/1‑150 (MQ=255)
   aTCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAaatcaatc                                                                                                                                                   >  2:639994/1‑150 (MQ=255)
      tGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATAAATctgc                                                                                                                                                >  1:128125/1‑150 (MQ=255)
       gTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATctgct                                                                                                                                               <  2:5141853/150‑1 (MQ=255)
       gTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATctgct                                                                                                                                               <  2:2788924/150‑1 (MQ=255)
        tttCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATctgctg                                                                                                                                              <  2:895706/150‑1 (MQ=255)
        tttCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATctgctg                                                                                                                                              >  1:3589663/1‑150 (MQ=255)
            ggAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTg                                                                                                                                          >  2:4535373/1‑150 (MQ=255)
              agaAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGAc                                                                                                                                        <  1:4939547/150‑1 (MQ=255)
                aaaCACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGt                                                                                                                                      <  1:2845737/150‑1 (MQ=255)
                 aaCACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGtt                                                                                                                                     >  1:5224904/1‑150 (MQ=255)
                  acacAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTg                                                                                                                                    >  2:3960247/1‑150 (MQ=255)
                   cacaGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATAAATCTGCTGCCTGACGTTGt                                                                                                                                   >  1:1309575/1‑150 (MQ=255)
                    acaGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTa                                                                                                                                  <  1:2932204/150‑1 (MQ=255)
                     caGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTAc                                                                                                                                 <  1:2420130/150‑1 (MQ=255)
                      aGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACa                                                                                                                                >  1:1609341/1‑150 (MQ=255)
                        tGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGt                                                                                                                              >  2:3473171/1‑150 (MQ=255)
                           aCGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGACCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATc                                                                                                                           >  1:3763121/1‑150 (MQ=255)
                             gTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATAAATCTGCTGCCTGACGTTGTACAGTATCtt                                                                                                                         >  1:4381128/1‑150 (MQ=255)
                               tAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTtta                                                                                                                       <  1:2686930/150‑1 (MQ=255)
                                aaTTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTttat                                                                                                                      <  1:463583/150‑1 (MQ=255)
                                 aTTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTttatt                                                                                                                     >  1:3278325/1‑150 (MQ=255)
                                  ttCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTttatta                                                                                                                    <  2:4965128/150‑1 (MQ=255)
                                   tCATCGTCAACTCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTtattat                                                                                                                   <  2:84658/150‑1 (MQ=255)
                                    cATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATc                                                                                                                  >  2:3248553/1‑150 (MQ=255)
                                     aTCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCa                                                                                                                 >  1:4850494/1‑150 (MQ=255)
                                     aTCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCa                                                                                                                 >  2:2457548/1‑150 (MQ=255)
                                        gTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACa                                                                                                              >  2:1042421/1‑150 (MQ=255)
                                         tCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACat                                                                                                             <  1:3716379/150‑1 (MQ=255)
                                          cAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACatg                                                                                                            <  2:1549658/150‑1 (MQ=255)
                                            aaTCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACatgat                                                                                                          <  2:1241365/150‑1 (MQ=255)
                                               cGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGAtggc                                                                                                       >  2:2913099/1‑150 (MQ=255)
                                                  ggCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGAtggctgg                                                                                                    >  1:2116683/1‑150 (MQ=255)
                                                         cGATTCTCGCCGCCCTGAAAACGCAGTTCACCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACTTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCgg                                                                                             >  2:4327833/1‑150 (MQ=255)
                                                          gATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGt                                                                                            >  2:5287043/1‑150 (MQ=255)
                                                           aTTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTc                                                                                           >  2:3780531/1‑150 (MQ=255)
                                                            ttCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTCTCTTTATTATCAACATGATGGCTGGCTTCCGGTcc                                                                                          >  1:899732/1‑150 (MQ=255)
                                                            ttCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTcc                                                                                          >  1:3438544/1‑150 (MQ=255)
                                                             tctcGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCt                                                                                         <  2:462065/150‑1 (MQ=255)
                                                                cgccgcCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGtt                                                                                      <  1:177000/150‑1 (MQ=255)
                                                                   cgccCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGGTGGCTGGCTTCCGGTCCTGTTTgg                                                                                   <  2:3887939/150‑1 (MQ=255)
                                                                   cgccCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCTGGTCCTGTTTgg                                                                                   >  1:4234151/1‑150 (MQ=255)
                                                                       cTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAac                                                                               >  2:4242412/1‑150 (MQ=255)
                                                                        tGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAact                                                                              >  1:4999691/1‑149 (MQ=255)
                                                                          aaaaCGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATAAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGACAacgac                                                                            <  1:4102666/150‑1 (MQ=255)
                                                                                  gTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGac                                                                    <  1:2436664/150‑1 (MQ=255)
                                                                                  gTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGac                                                                    >  1:8816/1‑150 (MQ=255)
                                                                                    tCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGacac                                                                  >  2:1323892/1‑150 (MQ=255)
                                                                                     ccccGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACt                                                                 <  1:5057659/150‑1 (MQ=255)
                                                                                     ccccGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACt                                                                 <  2:1464649/150‑1 (MQ=255)
                                                                                        cGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTaa                                                              >  2:2409166/1‑150 (MQ=255)
                                                                                         ggCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAAc                                                             >  2:1868736/1‑150 (MQ=255)
                                                                                             cgGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTc                                                         <  2:1141563/150‑1 (MQ=255)
                                                                                                 gCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATAAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTa                                                     <  2:1801125/150‑1 (MQ=255)
                                                                                                   tGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTAcg                                                   >  2:476132/1‑150 (MQ=255)
                                                                                                    ggATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATAAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTAcgc                                                  >  1:1016372/1‑150 (MQ=255)
                                                                                                        gAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGtt                                              <  2:4907281/150‑1 (MQ=255)
                                                                                                          agagCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTtta                                            <  1:1381028/150‑1 (MQ=255)
                                                                                                          agagCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTtta                                            <  1:1909599/150‑1 (MQ=255)
                                                                                                          agagCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTtta                                            <  2:5058528/150‑1 (MQ=255)
                                                                                                           gagCGACAAACGCCTGAACGGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTttat                                           <  2:2074661/150‑1 (MQ=255)
                                                                                                           gagCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTttat                                           >  1:1829816/1‑150 (MQ=255)
                                                                                                           gagCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTttat                                           <  2:2225762/150‑1 (MQ=255)
                                                                                                           gagCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATAAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTttat                                           <  1:838985/150‑1 (MQ=255)
                                                                                                            agCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTttatt                                          <  2:4002857/150‑1 (MQ=255)
                                                                                                            agCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTttatt                                          <  1:691379/150‑1 (MQ=255)
                                                                                                            agCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATAAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTttatt                                          <  1:2987670/150‑1 (MQ=255)
                                                                                                               gACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATg                                       <  1:288567/150‑1 (MQ=255)
                                                                                                               gACAAACGCCTGAACAGGTCACCAGTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATg                                       <  2:121569/150‑1 (MQ=255)
                                                                                                                 cAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGcc                                     <  2:1657812/150‑1 (MQ=255)
                                                                                                                 cAAACGCCTGAACAGGTCACCATTACGGTGAAAATAAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGcc                                     >  1:237522/1‑150 (MQ=255)
                                                                                                                   caCGCCTGCACAGGACACCACTACGGTGAAAATCAATCTGCGGCCAGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCt                                   <  1:3097654/149‑1 (MQ=255)
                                                                                                                    aCGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCtt                                  <  1:3490583/150‑1 (MQ=255)
                                                                                                                    aCGCCTGAACAGGTCACCATTACGGTGAAAATAAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCtt                                  <  1:1062904/150‑1 (MQ=255)
                                                                                                                     cGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCttt                                 <  1:2588397/150‑1 (MQ=255)
                                                                                                                     cGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGGACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGATTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCttt                                 >  2:3120731/1‑150 (MQ=255)
                                                                                                                      gCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTc                                >  2:4144068/1‑150 (MQ=255)
                                                                                                                         tGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAAt                             >  2:2697092/1‑150 (MQ=255)
                                                                                                                         tGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAAt                             <  1:3225706/150‑1 (MQ=255)
                                                                                                                            aCAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGa                          <  1:3960247/150‑1 (MQ=255)
                                                                                                                             cAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGaa                         <  2:609745/150‑1 (MQ=255)
                                                                                                                              aGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAg                        >  1:4510297/1‑150 (MQ=255)
                                                                                                                               ggTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAgg                       <  1:2285721/150‑1 (MQ=255)
                                                                                                                               ggTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAgg                       >  1:2543164/1‑150 (MQ=255)
                                                                                                                                gTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAggg                      <  1:1978983/150‑1 (MQ=255)
                                                                                                                                  cACCATTACGGTGAAAATAAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGc                    >  1:4692158/1‑150 (MQ=255)
                                                                                                                                    ccATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCg                  <  1:1179723/150‑1 (MQ=255)
                                                                                                                                     cATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGa                 >  2:763934/1‑150 (MQ=255)
                                                                                                                                       ttACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGaaa               >  1:604317/1‑150 (MQ=255)
                                                                                                                                        tACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGaaaa              >  1:366115/1‑150 (MQ=255)
                                                                                                                                          cGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAAt            <  1:693082/150‑1 (MQ=255)
                                                                                                                                           ggTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATg           <  1:1120055/150‑1 (MQ=255)
                                                                                                                                            gTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGc          <  2:1093047/150‑1 (MQ=255)
                                                                                                                                            gTGAAAATAAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGc          >  1:4186700/1‑150 (MQ=255)
                                                                                                                                              gAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTg        <  2:3010754/150‑1 (MQ=255)
                                                                                                                                               aaaaTCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTgg       >  1:3619619/1‑150 (MQ=255)
                                                                                                                                                  atcaatcTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGAtt    >  1:2497411/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGCATCTGTTTCGGAGAAACACAGTGAACGTTAATTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGACAAACGCCTGAACAGGTCACCATTACGGTGAAAATCAATCTGCTGCCTGACGTTGTACAGTATCTTTATTATCAACATGATGGCTGGCTTCCGGTCCTGTTTGGCAACGACGAGCGGACACTTAACGGTCATTACGCGGTTTATTATGCCCTTTCAATGGAAGGGGCCGAAAAATGCTGGATTGT  >  CP009273/2601823‑2602120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑