Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,665,772 G→T 100% E123* (GAA→TAA)  hcaD → phenylpropionate dioxygenase, ferredoxin reductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,665,7720GT99.8% 731.4 / ‑6.5 238E123* (GAA→TAA) hcaDphenylpropionate dioxygenase, ferredoxin reductase subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (113/123);  minor base A (2/0);  total (115/123)
Fisher's exact test for biased strand distribution p-value = 2.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 239 total aligned reads displayed.

TTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACA  >  CP009273/2665623‑2665918
                                                                                                                                                     |                                                                                                                                                   
  cATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCCGCGCGACCCCTGCCGTTGCTTGTTTCACAGGGATaa                                                                                                                                                   >  1:4726669/1‑150 (MQ=255)
  cATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATaa                                                                                                                                                   <  1:4384526/150‑1 (MQ=255)
   aTCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAAc                                                                                                                                                  <  1:1786147/150‑1 (MQ=255)
      tGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACgct                                                                                                                                               <  1:4412484/150‑1 (MQ=255)
      tGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACgct                                                                                                                                               <  2:4016486/150‑1 (MQ=255)
       gCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACgctg                                                                                                                                              <  2:3034301/150‑1 (MQ=255)
       gCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACGGGGGTAACgctg                                                                                                                                              >  1:2925147/1‑150 (MQ=255)
        cagtCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGGACTGGGATCAGCTTTTTAGAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACgctgc                                                                                                                                             <  2:4327678/147‑1 (MQ=255)
        cATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACgctgc                                                                                                                                             >  2:3454590/1‑150 (MQ=255)
           tCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCttt                                                                                                                                          <  2:567896/150‑1 (MQ=255)
           tCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCttt                                                                                                                                          <  2:5018222/150‑1 (MQ=255)
           tCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCttt                                                                                                                                          <  1:5015291/150‑1 (MQ=255)
            ccGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTa                                                                                                                                         <  1:4666888/150‑1 (MQ=255)
              ggTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCACGACCGCGGCCGTTGCTTGATGCACTGGGGTAAAGCGGCTTTTcc                                                                                                                                       >  1:1703416/1‑150 (MQ=255)
                tgtAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑t                                                                                                                                    <  2:1026906/150‑1 (MQ=255)
                tgtAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑t                                                                                                                                    <  2:3303150/150‑1 (MQ=255)
                 gtAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑Ta                                                                                                                                   <  2:3616484/150‑1 (MQ=255)
                   aaCCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCCGCGCGACCCCAGCCGGTGCGTGATGCACAGGGATAACGCTGCATTACCC‑AAcc                                                                                                                                 >  2:5131388/1‑149 (MQ=255)
                      cATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑AACGCCa                                                                                                                              >  2:2538760/1‑150 (MQ=255)
                      cATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCGGCTTTACCC‑TACGCCa                                                                                                                              >  2:282541/1‑150 (MQ=255)
                       aTCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCCCAGCCGTTGCGTGATGCACTGGGATAACGCTGCGTTACCC‑AACGccca                                                                                                                             >  2:1404696/1‑148 (MQ=255)
                         cAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGc                                                                                                                           >  2:3952810/1‑150 (MQ=255)
                              cGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATACCGGTg                                                                                                                      <  1:2988876/150‑1 (MQ=255)
                               gCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCCTTGCTTGATGCACAGGGATAACGCTGCTTTACCC‑AACGCCATGCCCGTGa                                                                                                                     >  2:5227470/1‑150 (MQ=255)
                               gCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCGCTGCCGGTTCTTGATGCACAGGGATTAAGCAGCGTTAACC‑AACGCCATTCCGGTGa                                                                                                                     >  2:2410646/1‑150 (MQ=255)
                                cTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGAt                                                                                                                    <  1:2898831/150‑1 (MQ=255)
                                 tGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCACGACCCCAGCCGGTGCTTGTTGCACAGGGATAAAGCTGCTTTTACC‑AACGCCAGGCCGGGGATg                                                                                                                   >  1:1280852/1‑150 (MQ=255)
                                   ggTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCGGCCGTTGCGTGATGCACGGGGATAACGCTGCGGTAGCC‑GAAGCCCTGCCGGGGCTgcc                                                                                                                 >  2:3657319/1‑150 (MQ=255)
                                    gTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATgccg                                                                                                                <  1:4496430/150‑1 (MQ=255)
                                        cgACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAg                                                                                                            <  2:3786093/150‑1 (MQ=255)
                                        cgACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCGGCCGGTGCTTGATTCACTGGGATAACGCTGGTTTAACC‑AACGCCATGCCGGGGAGgccgcccg                                                                                                            >  1:3723801/1‑148 (MQ=255)
                                           cacacGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACt                                                                                                         <  1:4899426/150‑1 (MQ=255)
                                           cacacGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACt                                                                                                         <  1:2623023/150‑1 (MQ=255)
                                           cacacGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACt                                                                                                         <  1:1621692/150‑1 (MQ=255)
                                              acGAGAGTTAGTGTTAACCAACGGCGACAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCg                                                                                                      <  1:963145/150‑1 (MQ=255)
                                              acGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCGGCCGTTGCATGATGCAATGGGATAACGCAGCGTTACCC‑AACGCCAAGCCGGGGATGCCCCCAAACTGCg                                                                                                      >  1:3537155/1‑150 (MQ=255)
                                                  gagTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCAt                                                                                                                             >  2:3113888/1‑123 (MQ=255)
                                                  gagTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCAt                                                                                                                             <  1:3113888/123‑1 (MQ=255)
                                                    gTTAGTGTCAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGt                                                                                                <  2:4259068/150‑1 (MQ=255)
                                                      tAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTc                                                                                              <  2:3873521/150‑1 (MQ=255)
                                                       aGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATCGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCt                                                                                             <  2:1209085/150‑1 (MQ=255)
                                                        gtgtTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCGCTGCCGGTGCGTGATGCACAGGGATAACGCGGCTTTTCCC‑AACGCCATTCCGGGGTTGCCGCCACACTGCGAGAACTTCTg                                                                                            >  2:2696180/1‑150 (MQ=255)
                                                         tgtTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGc                                                                                           <  1:123995/150‑1 (MQ=255)
                                                          gtTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCa                                                                                          <  1:3709558/150‑1 (MQ=255)
                                                          gtTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCACGACCCCTGCCGTTGGGTGATGCACTGGGGTAAAGCAGCGTTACAC‑AACCCCCAGCCGGGGAAGCCGCCCCAAAGCGAGAAGTTCTGCa                                                                                          >  1:613134/1‑150 (MQ=255)
                                                             aaCCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCCCTGCCGGTGCGTGATTCACTGGGATAACGCGGCGGTACCC‑GACGCCATGCCGGGGATGCCGCCCGACAGCGAGAAGTTATGCAGcc                                                                                       >  1:3025500/1‑150 (MQ=255)
                                                              aCCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCCGCTCGACCGCTGCCGTTGCTTGTTTCACTGGGTTAAAGCTGCTTTTCCC‑AACGCCATGCCGGTGGTGCCGCCAGACAGCGAGAAGTTATGCAGccc                                                                                      >  2:1137657/1‑150 (MQ=255)
                                                                cAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGTTAACGCGGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGa                                                                                    >  2:2139534/1‑150 (MQ=255)
                                                                 aaCGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGGCGCGCGACCCCAGCCGTTGCTTGATTGACAGGGTTTAAGCGGCGTTAACC‑AACGCCCAGCCCGTGATGCCGCCCGACAGCCAGAAGTTCAGCAGCCCCaa                                                                                   >  2:5093220/1‑150 (MQ=255)
                                                                 aaCGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCGCTGCCGTTTCGTGTTTCACAGGGATAAAGCTGCGTTTACC‑TACCCCCTGCCGGTGATGCCGCCAGACAGCGAGAACTTCAGCAGCCCCaa                                                                                   >  2:1068164/1‑150 (MQ=255)
                                                                   cGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACg                                                                                 <  1:1725665/150‑1 (MQ=255)
                                                                      cGAAAGCAGGCACTGGGATCAGCTTTTTAGAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTc                                                                              <  2:4487817/150‑1 (MQ=255)
                                                                        aaaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAg                                                                            <  2:1618486/150‑1 (MQ=255)
                                                                        aaaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAg                                                                            <  2:222151/150‑1 (MQ=255)
                                                                        aaaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACAGCGAGAAGTTCTGCAGCCCGAACGGTCAg                                                                            >  2:3261290/1‑150 (MQ=255)
                                                                         aaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGt                                                                           <  1:2527783/150‑1 (MQ=255)
                                                                         aaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCAGCCGTTGCTTGATGCACTGGGATAAAGCTGCTTTAAAC‑AACGCCAAGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGt                                                                           >  1:4382745/1‑150 (MQ=255)
                                                                         aaGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCCCAGCCGTTGCGTGATTCACGGGGATAACGCAGCTTTACCC‑TACCCCATGCCGGTGGTGCCGCCAGACAGCGAGAAGTTCATCAGCCCGAAAGGTCAGt                                                                           >  2:2098736/1‑150 (MQ=255)
                                                                           gCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCCGCGCGACCGCGGCCGGTGCGTTATTCACTGGGTTAAAGCGGCGTTTCCC‑AACGCCAAGCCGGTGATTCCGCCAGACAGCGAGAAGTTATGCAGCCCGAACGGTCAGGCg                                                                         >  1:1717872/1‑150 (MQ=255)
                                                                           gCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCg                                                                         <  1:860289/150‑1 (MQ=255)
                                                                              ggCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTAGAACGCTGCCGTTGCTTGATTCACTGGGATAACGCTGCTTTACCC‑TACGCCATTCCGGTGATGCCGCCAGACTGCTAGAAGTTCTGCAGCCCGAACGGTCAGTAGTGa                                                                      >  2:871613/1‑150 (MQ=255)
                                                                                  cTGGGATCATCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTAt                                                                  <  1:676210/150‑1 (MQ=255)
                                                                                  cTGGGATCAGCTTTTTATAGCAACCGGCGCGGCCCCGCGACCCCAGCCCGTTCGTGATTCACAGGGATAACCCAGCATTACCCAAACCCCAGCCCGGGGA‑GCCCCCACACAGCCCGAACATCAGCAGCCCCAACCGTCAGTCGTGAatat                                                                  >  2:1926559/1‑150 (MQ=255)
                                                                                  cTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTAt                                                                  <  1:2290542/150‑1 (MQ=255)
                                                                                  cTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTAt                                                                  <  2:2545936/150‑1 (MQ=255)
                                                                                   tGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATc                                                                 <  1:4044227/150‑1 (MQ=255)
                                                                                       aTCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCCCTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTg                                                             <  1:1060400/150‑1 (MQ=255)
                                                                                       aTCAGCTTTTTATAGCAACCGGCGCGGCAGCGCGACCCCAGCCGTTGCGTGATGCCCAGGGATAACGCGGCTTTTACC‑GACGCCCAGCCCGTGATTCCGCCACAAAGCGAGAACTTCTGCAACCCCAACGGGCAGTCGGGATTATCgggg                                                             >  2:4578361/1‑148 (MQ=255)
                                                                                         cAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGcc                                                           <  2:4101968/150‑1 (MQ=255)
                                                                                         cAGCTTTTTATAGCAACCGGCGCGGCAGCACGAACCCAGCCCTTGAATTATGCACTGGGGTAAACCTGCTTTAAAC‑AAACCCAAGACGGGTATTACGCCCCACAACGAGAACTTCTGCACCCCGCACGGTCAATACTGATTATAGGTggc                                                           >  2:5024555/1‑148 (MQ=255)
                                                                                          aGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTTCCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCAACAACTTCTGCACCCCCAACCGTCAGTCGTGAATATCGGTGCCg                                                          >  2:3917843/1‑150 (MQ=255)
                                                                                               tttATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACt                                                     <  1:1510368/150‑1 (MQ=255)
                                                                                                 tataGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTAt                                                   <  1:1617768/150‑1 (MQ=255)
                                                                                                 tataGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTAt                                                   <  1:2488887/150‑1 (MQ=255)
                                                                                                     gCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGt                                               <  1:5208675/150‑1 (MQ=255)
                                                                                                       aaCCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCt                                             <  2:2999106/150‑1 (MQ=255)
                                                                                                        aCCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTg                                            <  1:1732229/150‑1 (MQ=255)
                                                                                                         ccGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTgg                                           <  2:2423128/150‑1 (MQ=255)
                                                                                                               cgGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTgg                                     <  1:2668483/150‑1 (MQ=255)
                                                                                                                ggCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGc                                    <  2:1224565/150‑1 (MQ=255)
                                                                                                                 gCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCt                                   >  1:5270813/1‑150 (MQ=255)
                                                                                                                          ccGCTGCCGGTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGcc                          <  2:4291797/150‑1 (MQ=255)
                                                                                                                            gCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TCCGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAc                        <  2:3379915/150‑1 (MQ=255)
                                                                                                                            gCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAc                        <  1:3493819/150‑1 (MQ=255)
                                                                                                                            gCTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAc                        >  1:4612396/1‑150 (MQ=255)
                                                                                                                             cTGCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcg                       <  1:3789047/150‑1 (MQ=255)
                                                                                                                               gCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgca                     >  2:1694029/1‑150 (MQ=255)
                                                                                                                               gCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgca                     <  1:2242831/150‑1 (MQ=255)
                                                                                                                               gCCGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGAATATCGGTGCCGGAACTATAGGTCTGGAACTGGCTGCAAGCGCCAcgca                     >  1:4788201/1‑150 (MQ=255)
                                                                                                                                ccGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgcag                    >  2:4733439/1‑150 (MQ=255)
                                                                                                                                ccGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgcag                    >  2:1502230/1‑150 (MQ=255)
                                                                                                                                 cGTTGCTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCAcgcagc                   <  2:1514724/150‑1 (MQ=255)
                                                                                                                                      cTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGACCTGGCTGCCAGCGCCACGCAGCGCAGa              >  1:3182225/1‑150 (MQ=255)
                                                                                                                                      cTTGATGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGa              >  2:2901493/1‑150 (MQ=255)
                                                                                                                                          aTGCACTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTa          >  1:2507184/1‑150 (MQ=255)
                                                                                                                                             cACTGGGAAAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTCTTTGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAgg       >  2:1498605/1‑150 (MQ=255)
                                                                                                                                               cTGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTg     <  1:5131388/150‑1 (MQ=255)
                                                                                                                                                tGGGATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGa    >  2:70130/1‑150 (MQ=255)
                                                                                                                                                tGGGATAACGCTGATTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACAGCGAGAAGTTCTGCAGCCCGAACTGTCAGTCGAGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGgaga    >  2:828014/1‑150 (MQ=255)
                                                                                                                                                  ggATAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACa  >  1:4065141/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTCATCTGCATTCCGGTGTAACCATCAAAACGCTGGGTCGCGACACACGAGAGTTAGTGTTAACCAACGGCGAAAGCTGGCACTGGGATCAGCTTTTTATAGCAACCGGCGCGGCAGCTCGACCGCTGCCGTTGCTTGATGCACTGGGAGAACGCTGCTTTACCC‑TACGCCATGCCGGTGATGCCGCCAGACTGCGAGAAGTTCTGCAGCCCGAACGGTCAGTCGTGATTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACA  >  CP009273/2665623‑2665918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑